This page has only limited features, please log in for full access.
Accumulation of arsenic in plant tissues poses a substantial threat to global crop yields. The use of plant growth-promoting bacterial strains to mitigate heavy metal toxicity has been illustrated before. However, its potential to reduce plant arsenic uptake and toxicity has not been investigated to date. Here, we describe the identification and characterization of a Nocardiopsis lucentensis strain isolated from heavy metal contaminated soil. Inoculation with this bioactive actinomycete strain decreased arsenic root and shoot bioaccumulation in both C3 and C4 crop species namely barley and maize. Upon arsenate treatment, N. lucentensis S5 stimulated root citric acid production and the plant’s innate detoxification capacity in a species-specific manner. In addition, this specific strain promoted biomass gain, despite substantial tissue arsenic levels. Detoxification (metallothionein, phytochelatin, glutathione-S-transferase levels) was upregulated in arsenate-exposed shoot and roots, and this response was further enhanced upon S5 supplementation, particularly in barley and maize roots. Compared to barley, maize plants were more tolerant to arsenate-induced oxidative stress (less H2O2 and lipid peroxidation levels). However, barley plants invested more in antioxidative capacity induction (ascorbate-glutathione turnover) to mitigate arsenic oxidative stress, which was strongly enhanced by S5. We quantify and mechanistically discuss the physiological and biochemical basis of N. lucentensis-mediated plant biomass recovery on arsenate polluted soils. Our findings substantiate the potential applicability of a bactoremediation strategy to mitigate arsenic-induced yield loss in crops.
Hamada AbdElgawad; Gaurav Zinta; Walid Abuelsoud; Yasser M. Hassan; Dalal Hussien M. Alkhalifah; Wael N. Hozzein; Rafat Zrieq; Gerrit Ts Beemster; Sébastjen Schoenaers. An actinomycete strain of Nocardiopsis lucentensis reduces arsenic toxicity in barley and maize. Journal of Hazardous Materials 2021, 417, 126055 .
AMA StyleHamada AbdElgawad, Gaurav Zinta, Walid Abuelsoud, Yasser M. Hassan, Dalal Hussien M. Alkhalifah, Wael N. Hozzein, Rafat Zrieq, Gerrit Ts Beemster, Sébastjen Schoenaers. An actinomycete strain of Nocardiopsis lucentensis reduces arsenic toxicity in barley and maize. Journal of Hazardous Materials. 2021; 417 ():126055.
Chicago/Turabian StyleHamada AbdElgawad; Gaurav Zinta; Walid Abuelsoud; Yasser M. Hassan; Dalal Hussien M. Alkhalifah; Wael N. Hozzein; Rafat Zrieq; Gerrit Ts Beemster; Sébastjen Schoenaers. 2021. "An actinomycete strain of Nocardiopsis lucentensis reduces arsenic toxicity in barley and maize." Journal of Hazardous Materials 417, no. : 126055.
Fungal endophytes are a major source of anti-infective agents and other medically relevant compounds. However, their classical blinded-chemical investigation is a challenging process due to their highly complex chemical makeup. Thus, utilizing cheminformatics tools such as metabolomics and computer-aided modelling is of great help deal with such complexity and select the most probable bioactive candidates. In the present study, we have explored the fungal endophytes associated with the well-known antimalarial medicinal plant Artemisia annua for their production of further antimalarial agents. Based on the preliminary antimalarial screening of these endophytes and using LC-HRMS-based metabolomics and multivariate analyses, we suggested different potentially active metabolites (compounds 1–8). Further in silico investigation using the neural-network-based prediction software PASS led to the selection of a group of quinone derivatives (compounds 1–5) as the most possible active hits. Subsequent in vitro validation revealed emodin (1) and physcion (2) to be potent antimalarial candidates with IC50 values of 0.9 and 1.9 µM, respectively. Our approach in the present investigation therefore can be applied as a preliminary evaluation step in the natural products drug discovery, which in turn can facilitate the isolation of selected metabolites notably the biologically active ones.
Hani A. Alhadrami; Ahmed M. Sayed; Ahmed O. El-Gendy; Yara I. Shamikh; Yasser Gaber; Walid Bakeer; Noheir H. Sheirf; Eman Z. Attia; Gehan M. Shaban; Basma A. Khalifa; Che J. Ngwa; Gabriele Pradel; Mostafa E. Rateb; Hossam M. Hassan; Dalal H. M. Alkhalifah; Usama Ramadan Abdelmohsen; Wael N. Hozzein. A metabolomic approach to target antimalarial metabolites in the Artemisia annua fungal endophytes. Scientific Reports 2021, 11, 1 -11.
AMA StyleHani A. Alhadrami, Ahmed M. Sayed, Ahmed O. El-Gendy, Yara I. Shamikh, Yasser Gaber, Walid Bakeer, Noheir H. Sheirf, Eman Z. Attia, Gehan M. Shaban, Basma A. Khalifa, Che J. Ngwa, Gabriele Pradel, Mostafa E. Rateb, Hossam M. Hassan, Dalal H. M. Alkhalifah, Usama Ramadan Abdelmohsen, Wael N. Hozzein. A metabolomic approach to target antimalarial metabolites in the Artemisia annua fungal endophytes. Scientific Reports. 2021; 11 (1):1-11.
Chicago/Turabian StyleHani A. Alhadrami; Ahmed M. Sayed; Ahmed O. El-Gendy; Yara I. Shamikh; Yasser Gaber; Walid Bakeer; Noheir H. Sheirf; Eman Z. Attia; Gehan M. Shaban; Basma A. Khalifa; Che J. Ngwa; Gabriele Pradel; Mostafa E. Rateb; Hossam M. Hassan; Dalal H. M. Alkhalifah; Usama Ramadan Abdelmohsen; Wael N. Hozzein. 2021. "A metabolomic approach to target antimalarial metabolites in the Artemisia annua fungal endophytes." Scientific Reports 11, no. 1: 1-11.
Camellia sinensis (L.) O. Kuntze, commonly known as tea, is widely cultivated around the world in tropical and subtropical areas. Tea is mainly manufactured using young shoots of tea plants. Therefore, it is essential to control foliar diseases. Gray blight disease is caused by pestalotiopsis-like taxa and is known as one of the most destructive tea diseases. Although several studies have provided the groundwork for the fungal diseases associated with C. sinensis in Taiwan, gray blight disease has not been characterized based on diversity, molecular systematics, or pathogenicity. The goal of this study was to identify and characterize the causative agents of tea gray blight disease. A total of 98 pestalotiopsis-like isolates associated with symptomatic leaves of C. sinensis from major tea fields in Taiwan were investigated. Based on phylogenies of single and concatenated DNA sequences (internal transcribed spacer, β-tubulin, translation elongation factor 1-α) together with morphology, we resolved most of the pestalotiopsis-like species in this study. The study revealed seven well-classified taxa and seven tentative clades in three genera: Pestalotiopsis, Pseudopestalotiopsis, and Neopestalotiopsis. One novel species, Pseudopestalotiopsis annellata, was introduced. Five new records, Pseudopestalotiopsis chinensis, Pseudopestalotiopsis camelliae-sinensis, Pestalotiopsis camelliae, Pestalotiopsis yanglingensis, and Pestalotiopsis trachicarpicola, were introduced for the first time in Taiwan. Pseudopestalotiopsis chinensis was the taxon most frequently isolated from C. sinensis in this study. Furthermore, results of pathogenicity assessments exhibited that, with wound inoculation, all assayed isolates in this study were pathogenic on tea leaves. Pseudopestalotiopsis chinensis and Pseudopestalotiopsis camelliae-sinensis were identified as the major pathogens associated with gray blight disease of tea in Taiwan. To our knowledge, this is the first study of the diversity, pathogenicity, and characterization of pestalotiopsis-like fungi causing tea gray blight disease in Taiwan.
Miss Ichen Tsai; Chia-Lin Chung; Ms. Shiou-Ruei Lin; Ting-Hsuan Hung; Tang-Long Shen; Chih-Yi Hu; Wael N. Hozzein; Hiran Anjana Ariyawansa. Cryptic Diversity, Molecular Systematics, and Pathogenicity of Genus Pestalotiopsis and Allied Genera Causing Gray Blight Disease of Tea in Taiwan, With a Description of a New Pseudopestalotiopsis Species. Plant Disease 2021, 105, 425 -443.
AMA StyleMiss Ichen Tsai, Chia-Lin Chung, Ms. Shiou-Ruei Lin, Ting-Hsuan Hung, Tang-Long Shen, Chih-Yi Hu, Wael N. Hozzein, Hiran Anjana Ariyawansa. Cryptic Diversity, Molecular Systematics, and Pathogenicity of Genus Pestalotiopsis and Allied Genera Causing Gray Blight Disease of Tea in Taiwan, With a Description of a New Pseudopestalotiopsis Species. Plant Disease. 2021; 105 (2):425-443.
Chicago/Turabian StyleMiss Ichen Tsai; Chia-Lin Chung; Ms. Shiou-Ruei Lin; Ting-Hsuan Hung; Tang-Long Shen; Chih-Yi Hu; Wael N. Hozzein; Hiran Anjana Ariyawansa. 2021. "Cryptic Diversity, Molecular Systematics, and Pathogenicity of Genus Pestalotiopsis and Allied Genera Causing Gray Blight Disease of Tea in Taiwan, With a Description of a New Pseudopestalotiopsis Species." Plant Disease 105, no. 2: 425-443.
The arbuscular mycorrhizal (AM) fungi are a globally‐distributed group of soil organisms that play critical roles in ecosystem function. However, the ecological niches of individual AM fungal taxa are poorly understood. We collected >300 soil samples from natural ecosystems worldwide and modelled the realized niches of AM fungal virtual taxa (VT; approximately species‐level phylogroups). We found that environmental and spatial variables jointly explained VT distribution worldwide, with temperature and pH being the most important abiotic drivers, and spatial effects generally occurring at local‐ to regional‐scales. While dispersal limitation could explain some variation in VT distribution, VT relative abundance was almost exclusively driven by environmental variables. Several environmental and spatial effects on VT distribution and relative abundance were correlated with phylogeny, indicating that closely related VT exhibit similar niche optima and widths. Major clades within Glomeraceae exhibited distinct niche optima, Acaulosporaceae generally had niche optima in low pH and low temperature conditions, and Gigasporaceae generally had niche optima in high precipitation conditions. Identifying the realized niche space occupied by individual and phylogenetic groups of soil microbial taxa provides a basis for building detailed hypotheses about how soil communities respond to gradients and manipulation in ecosystems worldwide.
John Davison; Mari Moora; Marina Semchenko; Sakeenah Binte Adenan; Talaat Ahmed; Asem A. Akhmetzhanova; Juha M. Alatalo; Saleh Al‐Quraishy; Elena Andriyanova; Sten Anslan; Mohammad Bahram; Amgaa Batbaatar; Charlotte Brown; C. Guillermo Bueno; James Cahill; Juan José Cantero; Brenda B. Casper; Mikhail Cherosov; Saida Chideh; Ana P. Coelho; Matthew Coghill; Guillaume Decocq; Sergey Dudov; Ezequiel Chimbioputo Fabiano; Vladimir E. Fedosov; Lauchlan Fraser; Sydney I. Glassman; Aveliina Helm; Hugh A. L. Henry; Bruno Hérault; Indrek Hiiesalu; Inga Hiiesalu; Wael N. Hozzein; Petr Kohout; Urmas Kõljalg; Kadri Koorem; Lauri Laanisto; Ülo Mander; Ladislav Mucina; Jean‐Pierre Munyampundu; Lena Neuenkamp; Ülo Niinemets; Casper Nyamukondiwa; Jane Oja; Vladimir Onipchenko; Meelis Pärtel; Cherdchai Phosri; Sergei Põlme; Kersti Püssa; Argo Ronk; Alessandro Saitta; Olivia Semboli; Siim‐Kaarel Sepp; Alexey Seregin; Surya Sudheer; Clara P. Peña‐Venegas; Claudia Paz; Tanel Vahter; Martti Vasar; Annelies J. Veraart; Leho Tedersoo; Martin Zobel; Maarja Öpik. Temperature and pH define the realised niche space of arbuscular mycorrhizal fungi. New Phytologist 2021, 231, 763 -776.
AMA StyleJohn Davison, Mari Moora, Marina Semchenko, Sakeenah Binte Adenan, Talaat Ahmed, Asem A. Akhmetzhanova, Juha M. Alatalo, Saleh Al‐Quraishy, Elena Andriyanova, Sten Anslan, Mohammad Bahram, Amgaa Batbaatar, Charlotte Brown, C. Guillermo Bueno, James Cahill, Juan José Cantero, Brenda B. Casper, Mikhail Cherosov, Saida Chideh, Ana P. Coelho, Matthew Coghill, Guillaume Decocq, Sergey Dudov, Ezequiel Chimbioputo Fabiano, Vladimir E. Fedosov, Lauchlan Fraser, Sydney I. Glassman, Aveliina Helm, Hugh A. L. Henry, Bruno Hérault, Indrek Hiiesalu, Inga Hiiesalu, Wael N. Hozzein, Petr Kohout, Urmas Kõljalg, Kadri Koorem, Lauri Laanisto, Ülo Mander, Ladislav Mucina, Jean‐Pierre Munyampundu, Lena Neuenkamp, Ülo Niinemets, Casper Nyamukondiwa, Jane Oja, Vladimir Onipchenko, Meelis Pärtel, Cherdchai Phosri, Sergei Põlme, Kersti Püssa, Argo Ronk, Alessandro Saitta, Olivia Semboli, Siim‐Kaarel Sepp, Alexey Seregin, Surya Sudheer, Clara P. Peña‐Venegas, Claudia Paz, Tanel Vahter, Martti Vasar, Annelies J. Veraart, Leho Tedersoo, Martin Zobel, Maarja Öpik. Temperature and pH define the realised niche space of arbuscular mycorrhizal fungi. New Phytologist. 2021; 231 (2):763-776.
Chicago/Turabian StyleJohn Davison; Mari Moora; Marina Semchenko; Sakeenah Binte Adenan; Talaat Ahmed; Asem A. Akhmetzhanova; Juha M. Alatalo; Saleh Al‐Quraishy; Elena Andriyanova; Sten Anslan; Mohammad Bahram; Amgaa Batbaatar; Charlotte Brown; C. Guillermo Bueno; James Cahill; Juan José Cantero; Brenda B. Casper; Mikhail Cherosov; Saida Chideh; Ana P. Coelho; Matthew Coghill; Guillaume Decocq; Sergey Dudov; Ezequiel Chimbioputo Fabiano; Vladimir E. Fedosov; Lauchlan Fraser; Sydney I. Glassman; Aveliina Helm; Hugh A. L. Henry; Bruno Hérault; Indrek Hiiesalu; Inga Hiiesalu; Wael N. Hozzein; Petr Kohout; Urmas Kõljalg; Kadri Koorem; Lauri Laanisto; Ülo Mander; Ladislav Mucina; Jean‐Pierre Munyampundu; Lena Neuenkamp; Ülo Niinemets; Casper Nyamukondiwa; Jane Oja; Vladimir Onipchenko; Meelis Pärtel; Cherdchai Phosri; Sergei Põlme; Kersti Püssa; Argo Ronk; Alessandro Saitta; Olivia Semboli; Siim‐Kaarel Sepp; Alexey Seregin; Surya Sudheer; Clara P. Peña‐Venegas; Claudia Paz; Tanel Vahter; Martti Vasar; Annelies J. Veraart; Leho Tedersoo; Martin Zobel; Maarja Öpik. 2021. "Temperature and pH define the realised niche space of arbuscular mycorrhizal fungi." New Phytologist 231, no. 2: 763-776.
Deserts cover a significant proportion of the Earth’s surface and continue to expand as a consequence of climate change. Mutualistic arbuscular mycorrhizal (AM) fungi are functionally important plant root symbionts, and may be particularly important in drought stressed systems such as deserts. Here we provide a first molecular characterization of the AM fungi occurring in several desert ecosystems worldwide. We sequenced AM fungal DNA from soil samples collected from deserts in six different regions of the globe using the primer pair WANDA-AML2 with Illumina MiSeq. We recorded altogether 50 AM fungal phylotypes. Glomeraceae was the most common family, while Claroideoglomeraceae, Diversisporaceae and Acaulosporaceae were represented with lower frequency and abundance. The most diverse site, with 35 virtual taxa (VT), was in the Israeli Negev desert. Sites representing harsh conditions yielded relatively few reads and low richness estimates, for example, a Saudi Arabian desert site where only three Diversispora VT were recorded. The AM fungal taxa recorded in the desert soils are mostly geographically and ecologically widespread. However, in four sites out of six, communities comprised more desert-affiliated taxa (according to the MaarjAM database) than expected at random. AM fungal VT present in samples were phylogenetically clustered compared with the global taxon pool, suggesting that nonrandom assembly processes, notably habitat filtering, may have shaped desert fungal assemblages.
Martti Vasar; John Davison; Siim-Kaarel Sepp; Maarja Öpik; Mari Moora; Kadri Koorem; Yiming Meng; Jane Oja; Asem Akhmetzhanova; Saleh Al-Quraishy; Vladimir Onipchenko; Juan Cantero; Sydney Glassman; Wael Hozzein; Martin Zobel. Arbuscular Mycorrhizal Fungal Communities in the Soils of Desert Habitats. Microorganisms 2021, 9, 229 .
AMA StyleMartti Vasar, John Davison, Siim-Kaarel Sepp, Maarja Öpik, Mari Moora, Kadri Koorem, Yiming Meng, Jane Oja, Asem Akhmetzhanova, Saleh Al-Quraishy, Vladimir Onipchenko, Juan Cantero, Sydney Glassman, Wael Hozzein, Martin Zobel. Arbuscular Mycorrhizal Fungal Communities in the Soils of Desert Habitats. Microorganisms. 2021; 9 (2):229.
Chicago/Turabian StyleMartti Vasar; John Davison; Siim-Kaarel Sepp; Maarja Öpik; Mari Moora; Kadri Koorem; Yiming Meng; Jane Oja; Asem Akhmetzhanova; Saleh Al-Quraishy; Vladimir Onipchenko; Juan Cantero; Sydney Glassman; Wael Hozzein; Martin Zobel. 2021. "Arbuscular Mycorrhizal Fungal Communities in the Soils of Desert Habitats." Microorganisms 9, no. 2: 229.
The use of actinomycetes for improving soil fertility and plant production is an attractive strategy for developing sustainable agricultural systems due to their effectiveness, eco-friendliness, and low production cost. Out of 17 species isolated from the soil rhizosphere of legume crops, 4 bioactive isolates were selected and their impact on 5 legumes: soybean, kidney bean, chickpea, lentil, and pea were evaluated. According to the morphological and molecular identification, these isolates belong to the genus Streptomyces. Here, we showed that these isolates increased soil nutrients and organic matter content and improved soil microbial populations. At the plant level, soil enrichment with actinomycetes increased photosynthetic reactions and eventually increased legume yield. Actinomycetes also increased nitrogen availability in soil and legume tissue and seeds, which induced the activity of key nitrogen metabolizing enzymes, e.g., glutamine synthetase, glutamate synthase, and nitrate reductase. In addition to increased nitrogen-containing amino acids levels, we also report high sugar, organic acids, and fatty acids as well as antioxidant phenolics, mineral, and vitamins levels in actinomycete treated legume seeds, which in turn improved their seed quality. Overall, this study shed the light on the impact of actinomycetes on enhancing the quality and productivity of legume crops by boosting the bioactive primary and secondary metabolites. Moreover, our findings emphasize the positive role of actinomycetes in improving the soil by enriching its microbial population. Therefore, our data reinforce the usage of actinomycetes as biofertilizers to provide sustainable food production and achieve biosafety.
Hamada AbdelGawad; Walid Abuelsoud; Mahmoud M. Y. Madany; Samy Selim; Gaurav Zinta; Ahmed S. M. Mousa; Wael N. Hozzein. Actinomycetes Enrich Soil Rhizosphere and Improve Seed Quality as well as Productivity of Legumes by Boosting Nitrogen Availability and Metabolism. Biomolecules 2020, 10, 1675 .
AMA StyleHamada AbdelGawad, Walid Abuelsoud, Mahmoud M. Y. Madany, Samy Selim, Gaurav Zinta, Ahmed S. M. Mousa, Wael N. Hozzein. Actinomycetes Enrich Soil Rhizosphere and Improve Seed Quality as well as Productivity of Legumes by Boosting Nitrogen Availability and Metabolism. Biomolecules. 2020; 10 (12):1675.
Chicago/Turabian StyleHamada AbdelGawad; Walid Abuelsoud; Mahmoud M. Y. Madany; Samy Selim; Gaurav Zinta; Ahmed S. M. Mousa; Wael N. Hozzein. 2020. "Actinomycetes Enrich Soil Rhizosphere and Improve Seed Quality as well as Productivity of Legumes by Boosting Nitrogen Availability and Metabolism." Biomolecules 10, no. 12: 1675.
The aim of the present study was to evaluate mannan oligosaccharides (MOS) or glycerol (GLY) as a carbon source on biofloc systems of Nile tilapia (O. niloticus) juveniles. Fish (n = 750) were reared in open flow (Controls) or biofloc systems (B-GLY and B-MOS) fed with a plant or fish protein source over a period of twelve weeks. Total ammonia nitrogen and nitrate decreased in the biofloc groups, while biofloc volume increased in B-MOS. Compared to the controls, B-MOS and B-GLY exhibited higher weight gain and improved feed conversion, irrespectively of the diet. Serum level of C-reactive protein was reduced, while IgM and lysozyme activity was higher in the B-MOS fish, compared to other groups. Intestinal Bacillus spp. count was increased, whereas Vibrio, Aeromonas and Pseudomonas spp. counts decreased in B-MOS reared groups, compared to the other groups. The proinflammatory cytokine (IL-8 and IFN-γ) transcript expression was upregulated in B-MOS more than B-GLY reared groups. Compared to the controls, the virulence of Aeromonas hydrophila was decreased in the B-MOS and B-GLY groups. The results indicate several benefits of using MOS as a carbon source in a biofloc Nile tilapia system; a cost benefit analysis is required to assess the economic viability of this.
Asmaa T.Y. Kishawy; Alaa H. Sewid; Hend S. Nada; Mohamed A. Kamel; Shefaa A.M. El-Mandrawy; Taghrid M.N. Abdelhakim; Abd Elhakeem I. El-Murr; Nihal El Nahhas; Wael N. Hozzein; Doaa Ibrahim. Mannanoligosaccharides as a Carbon Source in Biofloc Boost Dietary Plant Protein and Water Quality, Growth, Immunity and Aeromonas hydrophila Resistance in Nile Tilapia (Oreochromis niloticus). Animals 2020, 10, 1724 .
AMA StyleAsmaa T.Y. Kishawy, Alaa H. Sewid, Hend S. Nada, Mohamed A. Kamel, Shefaa A.M. El-Mandrawy, Taghrid M.N. Abdelhakim, Abd Elhakeem I. El-Murr, Nihal El Nahhas, Wael N. Hozzein, Doaa Ibrahim. Mannanoligosaccharides as a Carbon Source in Biofloc Boost Dietary Plant Protein and Water Quality, Growth, Immunity and Aeromonas hydrophila Resistance in Nile Tilapia (Oreochromis niloticus). Animals. 2020; 10 (10):1724.
Chicago/Turabian StyleAsmaa T.Y. Kishawy; Alaa H. Sewid; Hend S. Nada; Mohamed A. Kamel; Shefaa A.M. El-Mandrawy; Taghrid M.N. Abdelhakim; Abd Elhakeem I. El-Murr; Nihal El Nahhas; Wael N. Hozzein; Doaa Ibrahim. 2020. "Mannanoligosaccharides as a Carbon Source in Biofloc Boost Dietary Plant Protein and Water Quality, Growth, Immunity and Aeromonas hydrophila Resistance in Nile Tilapia (Oreochromis niloticus)." Animals 10, no. 10: 1724.
In the present study, we investigated the actinomycetes associated with the Red Sea-derived soft coral Sarcophyton glaucum in terms of biological and chemical diversity. Three strains were cultivated and identified to be members of genera Micromonospora, Streptomyces, and Nocardiopsis; out of them, Micromonospora sp. UR17 was putatively characterized as a new species. In order to explore the chemical diversity of these actinobacteria as far as possible, they were subjected to a series of fermentation experiments under altering conditions, that is, solid and liquid fermentation along with co-fermentation with a mycolic acid-containing strain, namely Nocardia sp. UR23. Each treatment was found to affect these actinomycetes differently in terms of biological activity (i.e., antitrypanosomal activity) and chemical profiles evidenced by LC-HRES-MS-based metabolomics and multivariate analysis. Thereafter, orthogonal projections to latent structures discriminant analysis (OPLS-DA) suggested a number of metabolites to be associated with the antitrypanosomal activity of the active extracts. The subsequent in silico screenings (neural networking-based and docking-based) further supported the OPLS-DA results and prioritized desferrioxamine B (3), bafilomycin D (10), and bafilomycin A1 (11) as possible antitrypanosomal agents. Our approach in this study can be applied as a primary step in the exploration of bioactive natural products, particularly those from actinomycetes.
Noha M. Gamaleldin; Walid Bakeer; Ahmed M. Sayed; Yara I. Shamikh; Ahmed O. El-Gendy; Hossam M. Hassan; Hannes Horn; Usama Ramadan Abdelmohsen; Wael N. Hozzein. Exploration of Chemical Diversity and Antitrypanosomal Activity of Some Red Sea-Derived Actinomycetes Using the OSMAC Approach Supported by LC-MS-Based Metabolomics and Molecular Modelling. Antibiotics 2020, 9, 629 .
AMA StyleNoha M. Gamaleldin, Walid Bakeer, Ahmed M. Sayed, Yara I. Shamikh, Ahmed O. El-Gendy, Hossam M. Hassan, Hannes Horn, Usama Ramadan Abdelmohsen, Wael N. Hozzein. Exploration of Chemical Diversity and Antitrypanosomal Activity of Some Red Sea-Derived Actinomycetes Using the OSMAC Approach Supported by LC-MS-Based Metabolomics and Molecular Modelling. Antibiotics. 2020; 9 (9):629.
Chicago/Turabian StyleNoha M. Gamaleldin; Walid Bakeer; Ahmed M. Sayed; Yara I. Shamikh; Ahmed O. El-Gendy; Hossam M. Hassan; Hannes Horn; Usama Ramadan Abdelmohsen; Wael N. Hozzein. 2020. "Exploration of Chemical Diversity and Antitrypanosomal Activity of Some Red Sea-Derived Actinomycetes Using the OSMAC Approach Supported by LC-MS-Based Metabolomics and Molecular Modelling." Antibiotics 9, no. 9: 629.
Methicillin-resistant Staphylococcus aureus (MRSA) is a major human pathogen and a historically emergent zoonotic pathogen with public health and veterinary importance. In humans, MRSA commonly causes severe infectious diseases, including food poisoning, pyogenic endocarditis, suppurative pneumonia, otitis media, osteomyelitis, and pyogenic infections of the skin, soft tissues. In the horse, MRSA could cause a localized purulent infection and botryomycosis; in cattle and ewe, localized pyogenic infection and severe acute mastitis with marked toxemia; in sheep, abscess disease resembles caseous lymphadenitis caused by anaerobic strains; in dogs and cats, pustular dermatitis and food poisoning; in pig, exudative epidermatitis “greasy pig disease; in birds, MRSA causes bumble-foot. The methicillin resistance could be determined by PCR-based detection of the mecA gene as well as resistance to cefoxitin. In Egypt, MRSA is one of the important occasions of subclinical and clinical bovine mastitis, and the prevalence of MRSA varies by geographical region. In this review, we are trying to illustrate variable data about the host susceptibility, diseases, epidemiology, virulence factors, antibiotic resistance, treatment, and control of MRSA infection.
Abdelazeem M Algammal; Helal F Hetta; Amr Elkelish; Dalal Hussien H Alkhalifah; Wael N. Hozzein; Gaber El-Saber Batiha; Nihal El Nahhas; Mahmoud A Mabrok. Methicillin-Resistant Staphylococcus aureus (MRSA): One Health Perspective Approach to the Bacterium Epidemiology, Virulence Factors, Antibiotic-Resistance, and Zoonotic Impact. Infection and Drug Resistance 2020, ume 13, 3255 -3265.
AMA StyleAbdelazeem M Algammal, Helal F Hetta, Amr Elkelish, Dalal Hussien H Alkhalifah, Wael N. Hozzein, Gaber El-Saber Batiha, Nihal El Nahhas, Mahmoud A Mabrok. Methicillin-Resistant Staphylococcus aureus (MRSA): One Health Perspective Approach to the Bacterium Epidemiology, Virulence Factors, Antibiotic-Resistance, and Zoonotic Impact. Infection and Drug Resistance. 2020; ume 13 ():3255-3265.
Chicago/Turabian StyleAbdelazeem M Algammal; Helal F Hetta; Amr Elkelish; Dalal Hussien H Alkhalifah; Wael N. Hozzein; Gaber El-Saber Batiha; Nihal El Nahhas; Mahmoud A Mabrok. 2020. "Methicillin-Resistant Staphylococcus aureus (MRSA): One Health Perspective Approach to the Bacterium Epidemiology, Virulence Factors, Antibiotic-Resistance, and Zoonotic Impact." Infection and Drug Resistance ume 13, no. : 3255-3265.
Understanding the link between the protective role of potassium silicate (K2SiO3) against water shortage and the eventual grain yield of maize plants is still limited under semiarid conditions. Therefore, in this study, we provide insights into the underlying metabolic responses, mineral nutrients uptake and some nonenzymatic and enzymatic antioxidants that may differ in maize plants as influenced by the foliar application of K2SiO3 (0, 1 and 2 mM) under three drip irrigation regimes (100, 75 and 50% of water requirements). Our results indicated that, generally, plants were affected by both moderate and severe deficit irrigation levels. Deficit irrigation decreased shoot dry weight, root dry weight, leaf area index (LAI), relative water content (RWC), N, P, K, Ca, Fe, Zn, carotenoids, grain yield and its parameters, while root/shoot ratio, malondialdehyde (MDA), proline, soluble sugars, ascorbic acid, soluble phenols, peroxidase (POD), catalase (CAT), polyphenol oxidase (PPO), and ascorbate peroxidase (APX) were improved. The foliar applications of K2SiO3 relatively alleviated water stress-induced damage. In this respect, the treatment of 2 mM K2SiO3 was more effective than others and could be recommended to mitigate the effect of deficit irrigation on maize plants. Moreover, correlation analysis revealed a close link between yield and the most studied traits.
Mohamed F. M. Ibrahim; Gomaa Abd El-Samad; Hatem Ashour; Ahmed M. El-Sawy; Mohamed Hikal; Amr Elkelish; Hany Abd El-Gawad; Ahmed Abou El-Yazied; Wael N. Hozzein; Reham Farag. Regulation of Agronomic Traits, Nutrient Uptake, Osmolytes and Antioxidants of Maize as Influenced by Exogenous Potassium Silicate under Deficit Irrigation and Semiarid Conditions. Agronomy 2020, 10, 1212 .
AMA StyleMohamed F. M. Ibrahim, Gomaa Abd El-Samad, Hatem Ashour, Ahmed M. El-Sawy, Mohamed Hikal, Amr Elkelish, Hany Abd El-Gawad, Ahmed Abou El-Yazied, Wael N. Hozzein, Reham Farag. Regulation of Agronomic Traits, Nutrient Uptake, Osmolytes and Antioxidants of Maize as Influenced by Exogenous Potassium Silicate under Deficit Irrigation and Semiarid Conditions. Agronomy. 2020; 10 (8):1212.
Chicago/Turabian StyleMohamed F. M. Ibrahim; Gomaa Abd El-Samad; Hatem Ashour; Ahmed M. El-Sawy; Mohamed Hikal; Amr Elkelish; Hany Abd El-Gawad; Ahmed Abou El-Yazied; Wael N. Hozzein; Reham Farag. 2020. "Regulation of Agronomic Traits, Nutrient Uptake, Osmolytes and Antioxidants of Maize as Influenced by Exogenous Potassium Silicate under Deficit Irrigation and Semiarid Conditions." Agronomy 10, no. 8: 1212.
Most endemic plant species have limited altitudinal ranges. At higher altitudes, they are subjected to various environmental stresses. However, these plants use unique defense mechanisms at high altitudes as a convenient survival strategy. The changes in antioxidant defense system and accumulation of different secondary metabolites (SMs) were investigated as depending on altitude in five endemic endangered species (Nepeta septemcrenata, Origanum syriacum subsp. Sinaicum, Phlomis aurea, Rosa arabica, and Silene schimperiana) naturally growing in Saint Katherine protectorate (SKP). Leaves were collected from different sites between 1600 and 2200 m above sea level to assess the biochemical and physiological variations in response to high altitudes. At higher altitudes, the soil pH and micronutrient soil content decreased, which can be attributed to lower mineralization processes at lower pH. Total phenols, ascorbic acid, proline, flavonoids, and tannins increased in response to different altitudes. SMs progressively increased in the studied species, associated with a significant decrease in the levels of antioxidant enzyme activity. R. arabica, as the most threatened plant, showed the maximum response compared with other species. There was an increase in photosynthetic pigments, which was attained via the increase in chlorophyll a, chlorophyll b, and carotenoid contents. There was a significant increase in total soluble sugars and total soluble protein content in response to different altitudes. SDS-PAGE of leaf proteins showed alteration in the protein profile between different species and the same species grown at a different altitude. These five species can adapt to high-altitude habitats by various physiological mechanisms, which can provide a theoretical basis for the future conservation of these endangered endemic species in SKP.
Ahmed Hashim; Basmah Alharbi; Awatif Abdulmajeed; Amr Elkelish; Wael Hozzein; Heba Hassan. Oxidative Stress Responses of Some Endemic Plants to High Altitudes by Intensifying Antioxidants and Secondary Metabolites Content. Plants 2020, 9, 869 .
AMA StyleAhmed Hashim, Basmah Alharbi, Awatif Abdulmajeed, Amr Elkelish, Wael Hozzein, Heba Hassan. Oxidative Stress Responses of Some Endemic Plants to High Altitudes by Intensifying Antioxidants and Secondary Metabolites Content. Plants. 2020; 9 (7):869.
Chicago/Turabian StyleAhmed Hashim; Basmah Alharbi; Awatif Abdulmajeed; Amr Elkelish; Wael Hozzein; Heba Hassan. 2020. "Oxidative Stress Responses of Some Endemic Plants to High Altitudes by Intensifying Antioxidants and Secondary Metabolites Content." Plants 9, no. 7: 869.
Calf diarrhea is one of the considerable infectious diseases in calves, which results in tremendous economic losses globally. To determine the prevalence of Shiga-toxigenic E. coli (STEC) and Enterotoxigenic E. coli (ETEC) incriminated in calf diarrhea, with special reference to Shiga- toxins genes (stx1 and stx2) and enterotoxins genes (lt and sta) that govern their pathogenesis, as well as the virulence genes; eaeA (intimin) and f41(fimbrial adhesion), and the screening of their antibiogram and antimicrobial resistance genes; aadB, sul1, and bla-TEM, a total of 274 fecal samples were collected (April 2018–Feb 2019) from diarrheic calves at different farms in El-Sharqia Governorate, Egypt. The bacteriological examination revealed that the prevalence of E. coli in diarrheic calves was 28.8%. The serotyping of the isolated E. coli revealed 7 serogroups; O26, O128, O111, O125, O45, O119 and O91. Furthermore, the Congo red binding test was carried out, where 89.8% of the examined strains (n = 71) were positive. The antibiogram of the isolated strains was investigated; the majority of E. coli serotypes exhibit multidrug resistance (MDR) to four antimicrobial agents; neomycin, gentamycin, streptomycin, and amikacin. Polymerase chain reaction (PCR) was used to detect the prevalence of the virulence genes; stx1, stx2 lt, sta, f41 and eaeA, as well as the antimicrobial resistance genes; aadB, sul1, and bla-TEM. The prevalence of STEC was 20.2% (n = 16), while the prevalence of ETEC was 30.4% (n = 24). Briefly, the Shiga toxins genes; stx1 and stx2, are the most prevalent virulence genes associated with STEC, which are responsible for the pathogenesis of the disease and helped by the intimin gene (eaeA). In addition, the lt gene is the most prevalent enterotoxin gene accompanied by the ETEC strains, either alone or in combination with sta and/or f41 genes. The majority of pathogenic E. coli incriminated in calf diarrhea possesses the aadB resistance gene, followed by the sul1 gene. Enrofloxacin, florfenicol, amoxicillin-clavulanic acid, and ampicillin-sulbactam, are the most effective antimicrobial agents against the isolated STEC and ETEC strains.
Abdelazeem M. Algammal; Ali W. El-Kholy; Emad M. Riad; Hossam E. Mohamed; Mahmoud M. Elhaig; Sulaiman A. Al Yousef; Wael N. Hozzein; Madeha O. I. Ghobashy. Genes Encoding the Virulence and the Antimicrobial Resistance in Enterotoxigenic and Shiga-Toxigenic E. coli Isolated from Diarrheic Calves. Toxins 2020, 12, 383 .
AMA StyleAbdelazeem M. Algammal, Ali W. El-Kholy, Emad M. Riad, Hossam E. Mohamed, Mahmoud M. Elhaig, Sulaiman A. Al Yousef, Wael N. Hozzein, Madeha O. I. Ghobashy. Genes Encoding the Virulence and the Antimicrobial Resistance in Enterotoxigenic and Shiga-Toxigenic E. coli Isolated from Diarrheic Calves. Toxins. 2020; 12 (6):383.
Chicago/Turabian StyleAbdelazeem M. Algammal; Ali W. El-Kholy; Emad M. Riad; Hossam E. Mohamed; Mahmoud M. Elhaig; Sulaiman A. Al Yousef; Wael N. Hozzein; Madeha O. I. Ghobashy. 2020. "Genes Encoding the Virulence and the Antimicrobial Resistance in Enterotoxigenic and Shiga-Toxigenic E. coli Isolated from Diarrheic Calves." Toxins 12, no. 6: 383.
Two novel strains, designated SYSU L10167T and SYSU L10180T, were isolated from sediment sampled at Dabancheng saline lake in Xinjiang, PR China. A polyphasic approach was used to clarify the taxonomic positions of the two strains. Cells of the isolates were curved ring-like, horseshoe-shaped or rod-shaped, non-motile and non-spore-forming. Cells were Gram-stain-negative, aerobic, heterotrophic and rose-pigmented. The phylogenetic trees based on 16S rRNA gene sequences showed that strains SYSU L10167T and SYSU L10180T formed a distinct lineage within the genus Cyclobacterium . Strains SYSU L10167T and SYSU L10180T showed highest similarities to Cyclobacterium jeungdonense KCTC 23150T (98.0 and 97.4%, respectively). Results of genomic analyses (including average nucleotide identity, digital DNA–DNA hybridization and the marker gene tree) and pan-genome analysis further confirmed that strains SYSU L10167T and SYSU L10180T were separate from each other and other species of the genus Cyclobacterium . The draft genomes of the isolates had sizes of 5.5–5.7 Mb and reflected their major physiological capabilities. Based on phenotypic, physiological, chemotaxonomic and genotypic characterization, we propose that the isolates represent two novel species, for which the names Cyclobacterium salsum sp. nov. and Cyclobacterium roseum sp. nov. are proposed. The type strains of the species are SYSU L10167T (=KCTC 72390T=CGMCC 1.17521T) and SYSU L10180T (=KCTC 72391T=CGMCC 1.17278T).
Yong-Hong Liu; Yuan-Guo Xie; Li Li; Hong-Chen Jiang; Osama Abdalla Abdelshafy Mohamad; Wael Hozzein; Bao-Zhu Fang; Wen-Jun Li. Cyclobacterium salsum sp. nov. and Cyclobacterium roseum sp. nov., isolated from a saline lake. International Journal of Systematic and Evolutionary Microbiology 2020, 70, 3785 -3793.
AMA StyleYong-Hong Liu, Yuan-Guo Xie, Li Li, Hong-Chen Jiang, Osama Abdalla Abdelshafy Mohamad, Wael Hozzein, Bao-Zhu Fang, Wen-Jun Li. Cyclobacterium salsum sp. nov. and Cyclobacterium roseum sp. nov., isolated from a saline lake. International Journal of Systematic and Evolutionary Microbiology. 2020; 70 (6):3785-3793.
Chicago/Turabian StyleYong-Hong Liu; Yuan-Guo Xie; Li Li; Hong-Chen Jiang; Osama Abdalla Abdelshafy Mohamad; Wael Hozzein; Bao-Zhu Fang; Wen-Jun Li. 2020. "Cyclobacterium salsum sp. nov. and Cyclobacterium roseum sp. nov., isolated from a saline lake." International Journal of Systematic and Evolutionary Microbiology 70, no. 6: 3785-3793.
The diversity of actinomycetes associated with the marine sponge Coscinoderma mathewsi collected from Hurghada (Egypt) was studied. Twenty-three actinomycetes were separated and identified based on the 16S rDNA gene sequence analysis. Out of them, three isolates were classified as novel species of the genera Micromonospora, Nocardia, and Gordonia. Genome sequencing of actinomycete strains has revealed many silent biosynthetic gene clusters and has shown their exceptional capacity for the production of secondary metabolites, not observed under classical cultivation conditions. Therefore, the effect of mycolic-acid-containing bacteria or mycolic acid on the biosynthesis of cryptic natural products was investigated. Sponge-derived actinomycete Micromonospora sp. UA17 was co-cultured using liquid fermentation with two mycolic acid-containing actinomycetes (Gordonia sp. UA19 and Nocardia sp. UA 23), or supplemented with pure mycolic acid. LC-HRESIMS data were analyzed to compare natural production across all crude extracts. Micromonospora sp. UA17 was rich with isotetracenone, indolocarbazole, and anthracycline analogs. Some co-culture extracts showed metabolites such as a chlorocardicin, neocopiamycin A, and chicamycin B that were not found in the respective monocultures, suggesting a mycolic acid effect on the induction of cryptic natural product biosynthetic pathways. The antibacterial, antifungal, and antiparasitic activities for the different cultures extracts were also tested.
Yara I. Shamikh; Aliaa A. El Shamy; Yasser Gaber; Usama Ramadan Abdelmohsen; Hashem A. Madkour; Hannes Horn; Hossam M. Hassan; Abeer H. Elmaidomy; Dalal Hussien M. Alkhalifah; Wael N. Hozzein. Actinomycetes from the Red Sea Sponge Coscinoderma mathewsi: Isolation, Diversity, and Potential for Bioactive Compounds Discovery. Microorganisms 2020, 8, 783 .
AMA StyleYara I. Shamikh, Aliaa A. El Shamy, Yasser Gaber, Usama Ramadan Abdelmohsen, Hashem A. Madkour, Hannes Horn, Hossam M. Hassan, Abeer H. Elmaidomy, Dalal Hussien M. Alkhalifah, Wael N. Hozzein. Actinomycetes from the Red Sea Sponge Coscinoderma mathewsi: Isolation, Diversity, and Potential for Bioactive Compounds Discovery. Microorganisms. 2020; 8 (5):783.
Chicago/Turabian StyleYara I. Shamikh; Aliaa A. El Shamy; Yasser Gaber; Usama Ramadan Abdelmohsen; Hashem A. Madkour; Hannes Horn; Hossam M. Hassan; Abeer H. Elmaidomy; Dalal Hussien M. Alkhalifah; Wael N. Hozzein. 2020. "Actinomycetes from the Red Sea Sponge Coscinoderma mathewsi: Isolation, Diversity, and Potential for Bioactive Compounds Discovery." Microorganisms 8, no. 5: 783.
Background: In this era, worldwide interest has been directed towards using natural antioxidants to guard against drug side effects. Saussurea lappa is a famous medicinal plant with many biologically active compounds. Triamcinolone acetonide (TA) is an extensively used glucocorticoid. Hence, this study explored, for the first time, the possible beneficial effects of S. lappa ethanolic extract on TA-induced oxidative damage in the lung and spleen of rats. Methods: Five experimental groups were used: control group, S. lappa-treated group (600 mg/kg/day, orally), TA-treated group (40 mg/kg/twice/week I/P), S. lappa + TA co-treated group, and S. lappa/TA prophylactic group. Results: TA exposure significantly induced leukocytosis and neutrophilia. In addition, TA significantly reduced the levels of C-reactive protein, interleukin-12, tumor necrosis factor α, and immunoglobulins. Lung Caspase-3 overexpression and splenic CD8+ downregulation were also noted in the TA group. TA treatment significantly increased malondialdehyde concentration but reduced superoxide dismutase and glutathione peroxidase activities. S. lappa counteracted the TA oxidative and apoptotic effects. The best results were recorded in the prophylactic group. Conclusions: S. lappa has a remarkable protective effect via its anti-inflammatory, anti-apoptotic, and antioxidant capacity. Thus, it could be a candidate as a natural antioxidant to face glucocorticoid’s harmful side effects.
Ghada I. Abd El-Rahman; Amany Behairy; Nora M. Elseddawy; Gaber El-Saber Batiha; Wael N. Hozzein; Dina M. Khodeer; Yasmina M. Abd-Elhakim. Saussurea lappa Ethanolic Extract Attenuates Triamcinolone Acetonide-Induced Pulmonary and Splenic Tissue Damage in Rats via Modulation of Oxidative Stress, Inflammation, and Apoptosis. Antioxidants 2020, 9, 396 .
AMA StyleGhada I. Abd El-Rahman, Amany Behairy, Nora M. Elseddawy, Gaber El-Saber Batiha, Wael N. Hozzein, Dina M. Khodeer, Yasmina M. Abd-Elhakim. Saussurea lappa Ethanolic Extract Attenuates Triamcinolone Acetonide-Induced Pulmonary and Splenic Tissue Damage in Rats via Modulation of Oxidative Stress, Inflammation, and Apoptosis. Antioxidants. 2020; 9 (5):396.
Chicago/Turabian StyleGhada I. Abd El-Rahman; Amany Behairy; Nora M. Elseddawy; Gaber El-Saber Batiha; Wael N. Hozzein; Dina M. Khodeer; Yasmina M. Abd-Elhakim. 2020. "Saussurea lappa Ethanolic Extract Attenuates Triamcinolone Acetonide-Induced Pulmonary and Splenic Tissue Damage in Rats via Modulation of Oxidative Stress, Inflammation, and Apoptosis." Antioxidants 9, no. 5: 396.
Introduction: Copper oxide nanoparticles (CuO-NPs) are widely used as feed additives for livestock and poultry and implicated in many biomedical applications; however, overload of copper NPs induces various toxicological changes and dysfunction of animal’s organs. Thus, this study was designed to evaluate the comparative toxicological effects of biologically and chemically synthesized CuO-NPs on mice. Methods: Transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FT-IR) were used to characterize the sizes, shapes and functional groups of CuO-NPs. Forty-five mice were randomly allocated into three groups. Control group received distilled water. The second group was administered a single dose of biologically synthesized CuO-NPs (500 mg/kg bw) orally. The third group was administered a single dose of chemically synthesized CuO-NPs (500 mg/kg bw) orally. Results: TEM revealed that biologically synthesized NPs were spherical in shape, whereas chemically synthesized NPs were spherical or elongated in shape. XRD showed that the size of biologically synthesized NPs ranged from 4.14 to 12.82 nm and that of chemically synthesized NPs ranged from 4.06 to 26.82 nm. FT-IR spectroscopy indicated that the peaks appeared between 779 cm− 1 and 425 cm− 1 in biologically synthesized NPs and between 858 cm− 1 and 524 cm− 1 in chemically synthesized NPs were for Cu-O nanostructure. Four mice died due to administration of biologically synthesized CuO-NPs. Both biologically and chemically synthesized CuO-NPs induced leukocytosis, elevated serum activities of alanine aminotransferase and aspartate aminotransferase and serum levels of urea and creatinine and increased P53 mRNA and caspase-3 protein expressions in hepatic tissues. Moreover, CuO-NPs induced degenerative and necrotized changes in hepatic, renal and splenic tissues. Biochemical, apoptotic and pathological changes were more serious in mice administered with biologically synthesized CuO-NPs. Conclusion: This study indicated that a high dose of biologically and chemically synthesized CuO-NPs induced adverse effects on hepatic, renal and splenic tissues. At the same dose level, the biologically synthesized CuO-NPs evoked more potent toxic effects than the chemically synthesized CuO-NPs.
Badr E. El Bialy; Ragaa A Hamouda; Mabrouk A Abd Eldaim; Salah S El Ballal; HaniM S Heikal; Hanem K Khalifa; Wael N Hozzein. Comparative Toxicological Effects of Biologically and Chemically Synthesized Copper Oxide Nanoparticles on Mice. International Journal of Nanomedicine 2020, ume 15, 3827 -3842.
AMA StyleBadr E. El Bialy, Ragaa A Hamouda, Mabrouk A Abd Eldaim, Salah S El Ballal, HaniM S Heikal, Hanem K Khalifa, Wael N Hozzein. Comparative Toxicological Effects of Biologically and Chemically Synthesized Copper Oxide Nanoparticles on Mice. International Journal of Nanomedicine. 2020; ume 15 ():3827-3842.
Chicago/Turabian StyleBadr E. El Bialy; Ragaa A Hamouda; Mabrouk A Abd Eldaim; Salah S El Ballal; HaniM S Heikal; Hanem K Khalifa; Wael N Hozzein. 2020. "Comparative Toxicological Effects of Biologically and Chemically Synthesized Copper Oxide Nanoparticles on Mice." International Journal of Nanomedicine ume 15, no. : 3827-3842.
New generations of hyperbranched aramids were synthesized from diarylamine and methyl acrylate using an AB2 monomer approach in a straightforward one-pot preparation. The chemical structure of hyperbranched Phenylenediamine/Methyl Acrylate HB(PDMA was confirmed by Fourier Transform Infrared (FTIR) and Nuclear Magnetic Resonance (1HNMR) spectroscopy. In addition, the particle’s size and distribution were recorded using Dynamic Light Scattering (DLS). Moreover, the synthesized HB(PDMA)s displayed broad-spectrum antimicrobial activities against Gram-positive and Gram-negative bacteria as well as yeast strains and anti-biofilm activity where the highest activity was attributed to HB(PDMA)G4 at the lowest Minimum Inhibitory, Minimum Bactericidal, and Fungicidal Concentrations (MIC, MBC, and MFC, respectively). Furthermore, the HB(PDMA)s expressed anti-bacterial activity against isolated Pseudomonas sp. (R301) at a salinity of 35,000 ppm (NaCl). In addition, they revealed different corrosion inhibition efficiencies at the cultivated medium salinity at the estimated minimum bactericidal concentrations. The highest metal corrosion inhibition efficiencies were 59.5 and 94.3% for HB(PDMA)G4 at the Minimum Bactericidal Concentrations (MBCs) and two times Minimum Bactericidal Concentrations (2XMBCs), respectively, in comparison to both negative and positive controls.
Khalid I. Kabel; Ahmed Labena; Mohamed Keshawy; Wael N. Hozzein. Progressive Applications of Hyperbranched Polymer Based on Diarylamine: Antimicrobial, Anti-Biofilm and Anti-Aerobic Corrosion. Materials 2020, 13, 2076 .
AMA StyleKhalid I. Kabel, Ahmed Labena, Mohamed Keshawy, Wael N. Hozzein. Progressive Applications of Hyperbranched Polymer Based on Diarylamine: Antimicrobial, Anti-Biofilm and Anti-Aerobic Corrosion. Materials. 2020; 13 (9):2076.
Chicago/Turabian StyleKhalid I. Kabel; Ahmed Labena; Mohamed Keshawy; Wael N. Hozzein. 2020. "Progressive Applications of Hyperbranched Polymer Based on Diarylamine: Antimicrobial, Anti-Biofilm and Anti-Aerobic Corrosion." Materials 13, no. 9: 2076.
There is scarce information on cationic surfactants’ biocidal and corrosion inhbibition effects on Slime-Forming Bacteria (SFB) isolated from oil field formation water. Therefore, this work focused on the the synthesis of a cationic surfactant (CS) to increase its features by capping different metal nanoparticles (zinc, ZnNPs-C-CS; manganese, MnNPs-C-CS and tin, SnNPs-C-CS) and used them as biocides and corrosion inhibitors. The cationic surfactant was synthesized and characterized by Fourier-Transform Infrared (FTIR) and Nuclear Magnetic Resonance (NMR) spectroscopy. Afterwards, different nanoparticles were synthesized, characterized, and exploited to cap by the CS. The CS and the different nanoparticles capped by the CS were tested for their antimicrobial susceptibility against standard bacterial and yeast strains. The synthesized compounds were further evaluated as anti-biofilms agents against positively-developed bacterial biofilms. Moreover, the CS and the ZnNPs-C-CS, MnNPs-C-CS, and SnNPs-C-CS were assessed as potential biocides against SFB, particularly Pseudomonas sp. (isolated from contaminated formation water), and as corrosion inhibitors against cultivated salinity. The results revealed the great effect of the different CS-capped NPs as broad-spectrum antimicrobial and anti-biofilm agents at lower Minimum Inhibitory Concentrations (MICs), Minimum Bactericidal Concentrations (MBCs), Minimum Fungicidal Concentrations (MFCs) and Minimum Biofilm Inhibitory Concentrations (MBICs), and the activities were reported in order of SnNPs-C-CS > MnNPs-C-CS > ZnNPs-C-CS > CS. Furthermore, the ZnNPs-C-CS, MnNPs-C-CS, and SnNPs-C-CS demonstrated biocidal and corrosion inhibition effects against Pseudomonas sp. at a salinity of 3.5% NaCl, with metal corrosion inhibition efficiencies of 88.6, 94.0 and 96.9%, in comparison to a CS efficiency of 85.7%. In conclusion, the present work provides a newly synthesized cationic surfactant and has enhanced its antimicrobial and its metal corrosion inhibition effects by capping different nanoparticles, and it has been successfully applied against slime-forming bacteria at a salinity of 3.5% NaCl.
A. Labena; M. A. Hegazy; W. M. Kamel; Amr Elkelish; Wael N. Hozzein. Enhancement of A Cationic Surfactant by Capping Nanoparticles: Synthesis, Characterization and Multiple Applications. Molecules 2020, 25, 2007 .
AMA StyleA. Labena, M. A. Hegazy, W. M. Kamel, Amr Elkelish, Wael N. Hozzein. Enhancement of A Cationic Surfactant by Capping Nanoparticles: Synthesis, Characterization and Multiple Applications. Molecules. 2020; 25 (9):2007.
Chicago/Turabian StyleA. Labena; M. A. Hegazy; W. M. Kamel; Amr Elkelish; Wael N. Hozzein. 2020. "Enhancement of A Cationic Surfactant by Capping Nanoparticles: Synthesis, Characterization and Multiple Applications." Molecules 25, no. 9: 2007.
Balanites aegyptiaca L. is a multipurpose tree distributed in Africa and Middle East. Several parts of B. aegyptiaca have been suggested to have medicinal uses. So far the effect of ecological origin on the nutritional values and biological activities of B. aegyptiaca genotypes is rarely investigated. Further, metabolic profiling and assessment of the functional food value of B. aegyptiaca leaves are far from complete. In this study, biological activities and profiling of primary and secondary metabolites were investigated in the leaves of five B. aegyptiaca provenances collected from Egypt, Sudan, Saudi Arabia, and Yemen. Interestingly, all provenances showed notable antidiabetic, antioxidant, antiprotozoal, antibacterial, antifungal, and anticancer activities. Hierarchical clustering analysis revealed significant variability in the concentrations of individual sugars, organic acids, amino acids, fatty acids, vitamins, phenolics, and minerals among the provenances and these variations were provenance dependent. Medina provenance showed the heights diphenylpicrylhydrazyl (DPPH) scavenging and antifungal activities and was the most powerful against embryonic kidney adenocarcinoma and urinary bladder carcinoma cells. The highest inhibition against Escherichia coli and colon carcinoma cells was observed by Sudan and Cairo provenances. El-Kharga and Yemen provenances showed the greatest activity against Trypanosoma cruzi and hepatocellular and urinary bladder carcinoma. Therefore, leaves of B. aegyptiaca possess good nutritive and biological capacities and might have potential applications in the food and medical industries. However, the strength of such activities is significantly affected by the provenance. PRACTICAL APPLICATIONS: According to the national Research Council (NRC) of United States, Balanites aegyptiaca L. is recognized among the 24 priority lost crops of Africa. B. aegyptiaca leaves contain considerable amounts of primary metabolites (e.g., sugars, EAAs, USFAs) and secondary (e.g., phenolic acids and flavonoids) metabolites, vitamins, and macro and microelements. The obvious existence of these nutritionally and medicinally related compounds supports the functional food value of B. aegyptiaca leaves. Moreover, the present results revealed that B. aegyptiaca is not only a foliage dietary plant, but also could be considered as a valuable source for neutraceuticals, which support its pharmacological value. So far, this is the first report to explore, in detail, the functional food value of B. aegyptiaca leaves by presenting a clear image about its metabolic profiling and biological activities, and how the provenance factor could affect these values.
Galal Khamis; Ahmed M. Saleh; Talaat H. Habeeb; Wael N. Hozzein; Mohammed A. M. Wadaan; Jutta Papenbrock; Hamada AbdelGawad. Provenance effect on bioactive phytochemicals and nutritional and health benefits of the desert date Balanites aegyptiaca. Journal of Food Biochemistry 2020, 44, e13229 .
AMA StyleGalal Khamis, Ahmed M. Saleh, Talaat H. Habeeb, Wael N. Hozzein, Mohammed A. M. Wadaan, Jutta Papenbrock, Hamada AbdelGawad. Provenance effect on bioactive phytochemicals and nutritional and health benefits of the desert date Balanites aegyptiaca. Journal of Food Biochemistry. 2020; 44 (6):e13229.
Chicago/Turabian StyleGalal Khamis; Ahmed M. Saleh; Talaat H. Habeeb; Wael N. Hozzein; Mohammed A. M. Wadaan; Jutta Papenbrock; Hamada AbdelGawad. 2020. "Provenance effect on bioactive phytochemicals and nutritional and health benefits of the desert date Balanites aegyptiaca." Journal of Food Biochemistry 44, no. 6: e13229.
Wael N. Hozzein. Introductory Chapter: Metagenomics and Metagenomic Approaches. Metagenomics - Basics, Methods and Applications 2020, 1 .
AMA StyleWael N. Hozzein. Introductory Chapter: Metagenomics and Metagenomic Approaches. Metagenomics - Basics, Methods and Applications. 2020; ():1.
Chicago/Turabian StyleWael N. Hozzein. 2020. "Introductory Chapter: Metagenomics and Metagenomic Approaches." Metagenomics - Basics, Methods and Applications , no. : 1.