Plant growth-promoting rhizobacteria have been applied to different vegetable crops but there is still no information on the effect of bacterial biostimulant application under variable nutritional level on lettuce seedlings and their performance after transplanting in the field. This study aimed to evaluate the efficacy of a bacterial biostimulant to enhance growth and quality of lettuce seedlings fertigated with increasing nutrient rates and to assess the efficacy of these treatments on lettuce head production. Lettuce seedlings were inoculated with 1.5 g L⁻¹ of TNC Bactorr^S13 (a commercial biostimulant containing 1.3 × 10⁸ CFU g⁻¹ of Bacillus spp.) and fertigated with a nutrient solution containing 0, 1, 2, and 4 g L⁻¹ of NPK fertilizer (20-20-20). At the end of transplant production, the plants were evaluated for greenhouse cultivation. The effect of fertigation rate on seedling height, dry biomass, dry matter percentage, and water use efficiency was evident up to 2 g L⁻¹ of fertilizer in the non-inoculated seedlings, whereas fresh biomass and nitrogen use efficiency changed up to 4 g L⁻¹ of fertilizer. The use of the bacterial biostimulant modified seedling growth and its response to nutrient availability. The inoculation of the substrate with Bacillus spp. promoted plant growth and allowed seedlings to reach the highest height and biomass accumulation. The physiological age of lettuce seedlings showed a strong influence on plant growth and production after transplanting. The bacterial treatment positively affected the yield and nitrate content of lettuce plants.

Bacteria producers of plant growth-promoting (PGP) substances are responsible for the enhancement of plant development through several mechanisms. The purpose of the present work was to evaluate the PGP traits of 63 bacterial strains that were isolated from an anthropogenic soil, and obtained by modification of vertisols in the Sicily region (Italy) seven years after creation. The microorganisms were tested for the following PGP characteristics: indole acetic acid (IAA), NH₃, HCN and siderophore production, 1-aminocyclopropane-1-carboxylate deaminase activity (ACC) and phosphate solubilization. The results of principal component analysis (PCA) showed that Bacillus tequilensis SI 319, Brevibacterium frigoritolerans SI 433, Pseudomonas lini SI 287 and Pseudomonas frederiksbergensis SI 307 expressed high levels of IAA and production of ACC deaminase enzyme, while for the rest of traits analyzed the best performances were registered with Pseudomonas genus, in particular for the strains Pseudomonas atacamensis SI 443, Pseudomonas reinekei SI 441 and Pseudomonas granadensis SI 422 and SI 450. The in vitro screening provided enough evidence for future in vivo growth promotion tests of these eight strains.

Borage (Borago officinalis L.) is a hairy pubescent herb known throughout the world for its folk medicinal uses, as well as for many culinary uses. There is still little information on the cultivation needs of this species, especially for its use as vegetable crop and as fresh-cut produce. Hence, the aim of the research was to study the effects of agronomic practices on yield and quality of borage and on the storability as minimally-processed product. Two experiments were carried out in two consecutive years in order to evaluate the effect of plant density and plastic mulching on yield and quality of two borage accessions at harvest and during storage as minimally-processed produce for 14 days at 4 °C. The highest plant density (8 plants m²) determined the highest yield of plants and minimally-processed leaves with good quality retention during storage. Mulching had a positive effect on earliness, yield, and shelf life of minimally-processed leaves but also increased nitrate accumulation and reduced ascorbic acid content. Borage plants with lower spacing grown on mulched soil showed the best yield of plants and minimally-processed leaves irrespective of the borage accession tested. Borage plants can be used to produce minimally-processed entire leaves with good quality characteristics.

The criteria of “Distinctness, Uniformity and Stability” as well as a high “overall quality index” are used to register the Italian modern varieties to the national register. Differently, local conservation varieties can be certified under different EU Directives that facilitate, as an overall objective, the preservation of biodiversity and the containment of genetic erosion. In recent years, products derived from ancient grains are perceived to be healthier and more sustainable by consumers, especially in Italy, with consequent higher market prices. The ancient tetraploid wheat varieties registered in the national register of conservation varieties amount to 28, 24 of which are Sicilian. They are supposed to have wide genetic variability compared to modern ones, making them vulnerable to fraud because they are difficult to trace. It is therefore important to have tools able to discriminate between autochthonous Sicilian varieties. This can be completed by gluten proteins composition, which also provides information on the technological properties of derived products. Fifty-one accessions belonging to twenty-two ancient varieties of Sicilian tetraploid (mostly durum) wheat were analyzed. Although wide intra-accession and intra-varietal variability measurements were assessed, the gliadin pattern of bulks of seeds belonging to each variety was discriminatory. Moreover, differences in technological attitudes were found between landraces. This paves the way to use gluten protein patterns for traceability, allowing local farmers and producers to valorize their products and assure consumers regarding the transparency of the entire supply chain.

Borage (Borago officinalis L.) is a wild vegetable appreciated as a folk medicine and for culinary preparations. The introduction of borage as a specialized cultivation would allow for the diversification of vegetable crops and would widen the offerings of raw and minimally processed leafy vegetables. Thus, the aim of the research was to evaluate the quality and shelf-life of fresh-cut borage stored at different temperatures. Borage plants were grown during the autumn–winter season and immediately minimally processed after harvest. Fresh-cut borage leaves packed in sealed bags were stored at 2 or 6 °C for 21 d. Weight loss, total soluble solids (TSS), titratable acidity (TA), ascorbic acid, nitrates, leaf color characteristics and overall quality were determined through the storage period. Borage plants were deemed suitable for minimal processing. Storage temperature significantly influenced the rate of quality loss. Borage leaves had an initial nitrate content of 329.3 mg kg⁻¹ FW that was not affected by temperature or storage. TSS and TA were higher in leaves stored at 6 °C. TSS, TA and ascorbic acid content increased during storage. Minimally processed borage leaves stored at 2 °C had lower weight loss and leaf color modifications during storage and a longer shelf life than those stored at 6 °C, so were still marketable after 21 d of storage.