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To explore the SARS-CoV-2 pandemic in Algeria, a dataset comprising ninety-five genomes originating from SARS-CoV-2 sampled from Algeria and other countries worldwide, from 24 December 2019, through 4 March 2021, was thoroughly examined. While performing a multi-component analysis regarding the Algerian outbreak, the toolkit of phylogenetic, phylogeographic, haplotype, and genomic analysis were effectively implemented. We estimated the Time to the Most Recent Common Ancestor (TMRCA) in reference to the Algerian pandemic and highlighted the multiple introductions of the disease and the missing data depicted in the transmission loop. In addition, we emphasized the significant role played by local and international travels in disease dissemination. Most importantly, we unveiled mutational patterns, the effect of unique mutations on corresponding proteins, and the relatedness regarding the Algerian sequences to other sequences worldwide. Our results revealed individual amino-acid replacements such as the deleterious replacement A23T in the orf3a gene in Algeria_EPI_ISL_418241. Additionally, a connection between Algeria_EPI_ISL_420037 and sequences originating from the USA was observed through a USA characteristic amino-acid replacement T1004I in the nsp3 gene, found in the aforementioned Algerian sequence. Similarly, successful tracing could be established, such as Algeria/G37318-8849/2020|EPI_ISL_766863, which was imported from Saudi Arabia during the pilgrimage. Lastly, we assessed the Algerian mitigation measures regarding disease containment using statistical analyses.
Safia Zeghbib; Balázs Somogyi; Brigitta Zana; Gábor Kemenesi; Róbert Herczeg; Fawzi Derrar; Ferenc Jakab. The Algerian Chapter of SARS-CoV-2 Pandemic: An Evolutionary, Genetic, and Epidemiological Prospect. Viruses 2021, 13, 1525 .
AMA StyleSafia Zeghbib, Balázs Somogyi, Brigitta Zana, Gábor Kemenesi, Róbert Herczeg, Fawzi Derrar, Ferenc Jakab. The Algerian Chapter of SARS-CoV-2 Pandemic: An Evolutionary, Genetic, and Epidemiological Prospect. Viruses. 2021; 13 (8):1525.
Chicago/Turabian StyleSafia Zeghbib; Balázs Somogyi; Brigitta Zana; Gábor Kemenesi; Róbert Herczeg; Fawzi Derrar; Ferenc Jakab. 2021. "The Algerian Chapter of SARS-CoV-2 Pandemic: An Evolutionary, Genetic, and Epidemiological Prospect." Viruses 13, no. 8: 1525.
The natural hosts of Orthohantaviruses are rodents, soricomorphs and bats, and it is well known that they may cause serious or even fatal diseases among humans worldwide. The virus is persistent among animals and it is shed via urine, saliva and feces throughout the entirety of their lives. We aim to identify the effectiveness of hantavirus detection in rodent tissue samples and urine originating from naturally infected rodents. Initially, animals were trapped at five distinct locations throughout the Transdanubian region in Hungary. Lung, liver, kidney and urine samples were obtained from 163 deceased animals. All organs and urine were tested using nested reverse transcription polymerase chain reaction (nRT-PCR). Furthermore, sera were examined for IgG antibodies against Dobrava–Belgrade virus (DOBV) and Puumala virus (PUUV) by Western blot assay. IgG antibodies against hantaviruses and/or nucleic acid were detected in 25 (15.3%) cases. Among Apodemus, Myodes, and Microtus rodent species, DOBV, PUUV and Tula virus (TULV) were clearly identified. Amid the PCR-positive samples, the nucleic acid of the viruses was detected most effectively in the kidney (100%), while only 55% of screened lung tissues were positive. Interestingly, only three out of 20 rodent urine samples were positive when tested using nRT-PCR. Moreover, five rodents were seropositive without detectable virus nucleic acid in any of the tested organs.
Mónika Madai; Győző Horváth; Róbert Herczeg; Balázs Somogyi; Brigitta Zana; Fanni Földes; Gábor Kemenesi; Kornélia Kurucz; Henrietta Papp; Safia Zeghbib; Ferenc Jakab. Effectiveness Regarding Hantavirus Detection in Rodent Tissue Samples and Urine. Viruses 2021, 13, 570 .
AMA StyleMónika Madai, Győző Horváth, Róbert Herczeg, Balázs Somogyi, Brigitta Zana, Fanni Földes, Gábor Kemenesi, Kornélia Kurucz, Henrietta Papp, Safia Zeghbib, Ferenc Jakab. Effectiveness Regarding Hantavirus Detection in Rodent Tissue Samples and Urine. Viruses. 2021; 13 (4):570.
Chicago/Turabian StyleMónika Madai; Győző Horváth; Róbert Herczeg; Balázs Somogyi; Brigitta Zana; Fanni Földes; Gábor Kemenesi; Kornélia Kurucz; Henrietta Papp; Safia Zeghbib; Ferenc Jakab. 2021. "Effectiveness Regarding Hantavirus Detection in Rodent Tissue Samples and Urine." Viruses 13, no. 4: 570.
Acute myocardial infarction (MI) is one of the most common causes of death worldwide. Pituitary adenylate cyclase activating polypeptide (PACAP) is a cardioprotective neuropeptide expressing its receptors in the cardiovascular system. The aim of our study was to examine tissue PACAP-38 in a translational porcine MI model and plasma PACAP-38 levels in patients with ST-segment elevation myocardial infarction (STEMI). Significantly lower PACAP-38 levels were detected in the non-ischemic region of the left ventricle (LV) in MI heart compared to the ischemic region of MI-LV and also to the Sham-operated LV in porcine MI model. In STEMI patients, plasma PACAP-38 level was significantly higher before percutaneous coronary intervention (PCI) compared to controls, and decreased after PCI. Significant negative correlation was found between plasma PACAP-38 and troponin levels. Furthermore, a significant effect was revealed between plasma PACAP-38, hypertension and HbA1c levels. This was the first study showing significant changes in cardiac tissue PACAP levels in a porcine MI model and plasma PACAP levels in STEMI patients. These results suggest that PACAP, due to its cardioprotective effects, may play a regulatory role in MI and could be a potential biomarker or drug target in MI.
Dora Szabo; Zsolt Sarszegi; Beata Polgar; Eva Saghy; Adam Nemeth; Dora Reglodi; Andras Makkos; Aniko Gorbe; Zsuzsanna Helyes; Peter Ferdinandy; Robert Herczeg; Attila Gyenesei; Attila Cziraki; Andrea Tamas. PACAP-38 in Acute ST-Segment Elevation Myocardial Infarction in Humans and Pigs: A Translational Study. International Journal of Molecular Sciences 2021, 22, 2883 .
AMA StyleDora Szabo, Zsolt Sarszegi, Beata Polgar, Eva Saghy, Adam Nemeth, Dora Reglodi, Andras Makkos, Aniko Gorbe, Zsuzsanna Helyes, Peter Ferdinandy, Robert Herczeg, Attila Gyenesei, Attila Cziraki, Andrea Tamas. PACAP-38 in Acute ST-Segment Elevation Myocardial Infarction in Humans and Pigs: A Translational Study. International Journal of Molecular Sciences. 2021; 22 (6):2883.
Chicago/Turabian StyleDora Szabo; Zsolt Sarszegi; Beata Polgar; Eva Saghy; Adam Nemeth; Dora Reglodi; Andras Makkos; Aniko Gorbe; Zsuzsanna Helyes; Peter Ferdinandy; Robert Herczeg; Attila Gyenesei; Attila Cziraki; Andrea Tamas. 2021. "PACAP-38 in Acute ST-Segment Elevation Myocardial Infarction in Humans and Pigs: A Translational Study." International Journal of Molecular Sciences 22, no. 6: 2883.
Background: Radiation therapy has undergone significant technical development in the past decade. However, the complex therapy of intermediate-risk patients with organ-confined prostate carcinoma still poses many questions. Our retrospective study investigated the impact of selected components of the treatment process including radiotherapy, hormone deprivation, risk classification, and patients’ response to therapy. Methods: The impact of delivered dose, planning accuracy, duration of hormone deprivation, risk classification, and the time to reach prostate-specific antigen (PSA) nadir state were analyzed among ninety-nine individuals afflicted with organ-confined disease. Progression was defined as a radiological or biochemical relapse within five years from radiotherapy treatment. Results: We found that 58.3% of the progressive population consisted of intermediate-risk patients. The progression rate in the intermediate group was higher (21.9%) than in the high-risk population (12.1%). Dividing the intermediate group, according to the International Society of Urological Pathology (ISUP) recommendations, resulted in the non-favorable subgroup having the highest rate of progression (33.3%) and depicting the lowest percentage of progression-free survival (66.7%). Conclusion: Extended pelvic irradiation on the regional lymph nodes may be necessary for the ISUP Grade 3 subgroup, similarly to the high-risk treatment. Therapy optimization regarding the intermediate-risk population based on the ISUP subgrouping suggestions is highly recommended in the treatment of organ-confined prostate cancer.
Viktória Temesfői; Róbert Herczeg; Zoltán Lőcsei; Klára Sebestyén; Zsolt Sebestyén; László Mangel; Miklós Damásdi. Should We Reconsider the Necessity of a Refinement of Prostate Cancer Risk Classification and Radiotherapy Treatment Strategy? Experiences from a Retrospective Analysis of Data from a Single Institution. Journal of Clinical Medicine 2020, 10, 110 .
AMA StyleViktória Temesfői, Róbert Herczeg, Zoltán Lőcsei, Klára Sebestyén, Zsolt Sebestyén, László Mangel, Miklós Damásdi. Should We Reconsider the Necessity of a Refinement of Prostate Cancer Risk Classification and Radiotherapy Treatment Strategy? Experiences from a Retrospective Analysis of Data from a Single Institution. Journal of Clinical Medicine. 2020; 10 (1):110.
Chicago/Turabian StyleViktória Temesfői; Róbert Herczeg; Zoltán Lőcsei; Klára Sebestyén; Zsolt Sebestyén; László Mangel; Miklós Damásdi. 2020. "Should We Reconsider the Necessity of a Refinement of Prostate Cancer Risk Classification and Radiotherapy Treatment Strategy? Experiences from a Retrospective Analysis of Data from a Single Institution." Journal of Clinical Medicine 10, no. 1: 110.
Severe Acute Respiratory Syndrome Coronavirus 2 is the third highly pathogenic human coronavirus in history. Since the emergence in Hubei province, China, during late 2019, the situation evolved to pandemic level. Following China, Europe was the second epicenter of the pandemic. To better comprehend the detailed founder mechanisms of the epidemic evolution in Central-Eastern Europe, particularly in Hungary, we determined the full-length SARS-CoV-2 genomes from 32 clinical samples collected from laboratory confirmed COVID-19 patients over the first month of disease in Hungary. We applied a haplotype network analysis on all available complete genomic sequences of SARS-CoV-2 from GISAID database as of 21 April 2020. We performed additional phylogenetic and phylogeographic analyses to achieve the recognition of multiple and parallel introductory events into our region. Here, we present a publicly available network imaging of the worldwide haplotype relations of SARS-CoV-2 sequences and conclude the founder mechanisms of the outbreak in Central-Eastern Europe.
Gábor Kemenesi; Safia Zeghbib; Balázs A Somogyi; Gábor Endre Tóth; Krisztián Bányai; Norbert Solymosi; Peter M Szabo; István Szabó; Ádám Bálint; Péter Urbán; Róbert Herczeg; Attila Gyenesei; Ágnes Nagy; Csaba István Pereszlényi; Gergely Csaba Babinszky; Gábor Dudás; Gabriella Terhes; Viktor Zöldi; Róbert Lovas; Szabolcs Tenczer; László Kornya; Ferenc Jakab. Multiple SARS-CoV-2 Introductions Shaped the Early Outbreak in Central Eastern Europe: Comparing Hungarian Data to a Worldwide Sequence Data-Matrix. Viruses 2020, 12, 1401 .
AMA StyleGábor Kemenesi, Safia Zeghbib, Balázs A Somogyi, Gábor Endre Tóth, Krisztián Bányai, Norbert Solymosi, Peter M Szabo, István Szabó, Ádám Bálint, Péter Urbán, Róbert Herczeg, Attila Gyenesei, Ágnes Nagy, Csaba István Pereszlényi, Gergely Csaba Babinszky, Gábor Dudás, Gabriella Terhes, Viktor Zöldi, Róbert Lovas, Szabolcs Tenczer, László Kornya, Ferenc Jakab. Multiple SARS-CoV-2 Introductions Shaped the Early Outbreak in Central Eastern Europe: Comparing Hungarian Data to a Worldwide Sequence Data-Matrix. Viruses. 2020; 12 (12):1401.
Chicago/Turabian StyleGábor Kemenesi; Safia Zeghbib; Balázs A Somogyi; Gábor Endre Tóth; Krisztián Bányai; Norbert Solymosi; Peter M Szabo; István Szabó; Ádám Bálint; Péter Urbán; Róbert Herczeg; Attila Gyenesei; Ágnes Nagy; Csaba István Pereszlényi; Gergely Csaba Babinszky; Gábor Dudás; Gabriella Terhes; Viktor Zöldi; Róbert Lovas; Szabolcs Tenczer; László Kornya; Ferenc Jakab. 2020. "Multiple SARS-CoV-2 Introductions Shaped the Early Outbreak in Central Eastern Europe: Comparing Hungarian Data to a Worldwide Sequence Data-Matrix." Viruses 12, no. 12: 1401.
To explore the SARS-CoV-2 early pandemic in Algeria, a dataset comprising forty-three genomes originating from SARS-CoV-2 sampled from Algeria and other countries worldwide, from 24 December 2019 through 8 March 2020, of which, were thoroughly examined. While performing a multi-component analysis regarding the Algerian outbreak, the toolkit of phylogenetic, phylodynamic, haplotype analyses and genomic analysis were effectively implemented. We estimated the TMRCA in reference to the Algerian pandemic and highlighted both the introduction of the disease originating in France and the missing data depicted in the transmission loop. Most importantly, we unveiled mutational patterns, recombination events and the relatedness regarding the Algerian sequences to the dataset. Our results revealed the unique amino-acid replacement L129F in the orf3a gene in Algeria_EPI_ISL_418241. Additionally, a connection between Algeria_EPI_ISL_420037 and sequences originating from the USA was observed through a USA characteristic amino-acid replacement T1004I in the nsp3 gene, found in the aforementioned Algerian sequence. Lastly, we assessed the Algerian mitigation measures regarding disease containment using statistical analyses.Author summaryA novel human coronavirus, specifically SARS-CoV-2, emerged in China in late 2019. In Algeria, the contact tracing revealed the introduction of the disease originated in France, however, the intricate dynamics regarding the disease remain unexplored. In this study, we attempt to portray our perspective regarding the evolutionary, genetic and epidemiological aspects of the early pandemic in Algeria during the spring of 2020, through the use of time scaled phylogeny, phylodynamic and mutational pattern characterization and exploration. Additionally, we assessed the efficiency of the implemented mitigation measures using statistical analysis. The results supported the virus introduction from France and highlighted an Algerian characteristic amino-acid replacement in addition to a relatedness to the USA sequences. Moreover, we revealed an indirect contamination among the three sampled patients. Therefore, our analysis is a starting point for further investigations and emphasize the importance regarding intensive sequencing and genome exploration for mitigation measures implementation, and both drug and vaccine development.
Safia Zeghbib; Balázs Somogyi; Brigitta Zana; Gábor Kemenesi; Róbert Herczeg; Fawzi Derrar; Ferenc Jakab. The Algerian chapter of SARS-CoV-2 pandemic: An evolutionary, genetic, and epidemiological prospect of the first wave. 2020, 1 .
AMA StyleSafia Zeghbib, Balázs Somogyi, Brigitta Zana, Gábor Kemenesi, Róbert Herczeg, Fawzi Derrar, Ferenc Jakab. The Algerian chapter of SARS-CoV-2 pandemic: An evolutionary, genetic, and epidemiological prospect of the first wave. . 2020; ():1.
Chicago/Turabian StyleSafia Zeghbib; Balázs Somogyi; Brigitta Zana; Gábor Kemenesi; Róbert Herczeg; Fawzi Derrar; Ferenc Jakab. 2020. "The Algerian chapter of SARS-CoV-2 pandemic: An evolutionary, genetic, and epidemiological prospect of the first wave." , no. : 1.
Severe Acute Respiratory Syndrome Coronavirus 2 is the third highly pathogenic human coronavirus in history. Since the emergence in Hubei province, China, during late 2019 the situation evolved to pandemic level. Following China, Europe was the second epicenter of the pandemic. To better comprehend the detailed founder mechanisms of the epidemic evolution in Central-Eastern Europe, particularly in Hungary, we determined the full-length SARS-CoV-2 genomes from 32 clinical samples collected from laboratory confirmed COVID-19 patients over the first month of disease in Hungary. We applied a haplotype network analysis on all available complete genomic sequences of SARS-CoV-2 from GISAID database as of the 21th of April, 2020. We performed additional phylogenetic and phylogeographic analyses to achieve the recognition of multiple and parallel introductory events into our region. Here we present a publicly available network imaging of the worldwide haplotype relations of SARS-CoV-2 sequences and conclude the founder mechanisms of the outbreak in Central-Eastern Europe.
Gábor Kemenesi; Safia Zeghbib; Balázs A Somogyi; Gábor Endre Tóth; Krisztián Bányai; Norbert Solymosi; Peter M Szabo; István Szabó; Ádám Bálint; Péter Urbán; Róbert Herczeg; Attila Gyenesei; Ágnes Nagy; Csaba István Pereszlényi; Gergely Csaba Babinszky; Gábor Dudás; Gabriella Terhes; Viktor Zöldi; Róbert Lovas; Szabolcs Tenczer; László Kornya; Ferenc Jakab. Multiple SARS-CoV-2 introductions shaped the early outbreak in Central Eastern Europe: comparing Hungarian data to a worldwide sequence data-matrix. 2020, 1 .
AMA StyleGábor Kemenesi, Safia Zeghbib, Balázs A Somogyi, Gábor Endre Tóth, Krisztián Bányai, Norbert Solymosi, Peter M Szabo, István Szabó, Ádám Bálint, Péter Urbán, Róbert Herczeg, Attila Gyenesei, Ágnes Nagy, Csaba István Pereszlényi, Gergely Csaba Babinszky, Gábor Dudás, Gabriella Terhes, Viktor Zöldi, Róbert Lovas, Szabolcs Tenczer, László Kornya, Ferenc Jakab. Multiple SARS-CoV-2 introductions shaped the early outbreak in Central Eastern Europe: comparing Hungarian data to a worldwide sequence data-matrix. . 2020; ():1.
Chicago/Turabian StyleGábor Kemenesi; Safia Zeghbib; Balázs A Somogyi; Gábor Endre Tóth; Krisztián Bányai; Norbert Solymosi; Peter M Szabo; István Szabó; Ádám Bálint; Péter Urbán; Róbert Herczeg; Attila Gyenesei; Ágnes Nagy; Csaba István Pereszlényi; Gergely Csaba Babinszky; Gábor Dudás; Gabriella Terhes; Viktor Zöldi; Róbert Lovas; Szabolcs Tenczer; László Kornya; Ferenc Jakab. 2020. "Multiple SARS-CoV-2 introductions shaped the early outbreak in Central Eastern Europe: comparing Hungarian data to a worldwide sequence data-matrix." , no. : 1.
A large percentage of primary sensory neurons in the trigeminal ganglia (TG) contain neuropeptides such as tachykinins or calcitonin gene-related peptide. Neuropeptides released from the central terminals of primary afferents sensitize the secondary nociceptive neurons in the trigeminal nucleus caudalis (TNC), but also activate glial cells contributing to neuroinflammation and consequent sensitization in chronic orofacial pain and migraine. In the present study, we investigated the newest member of the tachykinin family, hemokinin-1 (HK-1) encoded by the Tac4 gene in the trigeminal system. HK-1 had been shown to participate in inflammation and hyperalgesia in various models, but its role has not been investigated in orofacial pain or headache. In the complete Freund’s adjuvant (CFA)-induced inflammatory orofacial pain model, we showed that Tac4 expression increased in the TG in response to inflammation. Duration-dependent Tac4 upregulation was associated with the extent of the facial allodynia. Tac4 was detected in both TG neurons and satellite glial cells (SGC) by the ultrasensitive RNAscope in situ hybridization. We also compared gene expression changes of selected neuronal and glial sensitization and neuroinflammation markers between wild-type and Tac4-deficient (Tac4-/-) mice. Expression of the SGC/astrocyte marker in the TG and TNC was significantly lower in intact and saline/CFA-treated Tac4-/- mice. The procedural stress-related increase of the SGC/astrocyte marker was also strongly attenuated in Tac4-/- mice. Analysis of TG samples with a mouse neuroinflammation panel of 770 genes revealed that regulation of microglia and cytotoxic cell-related genes were significantly different in saline-treated Tac4-/- mice compared to their wild-types. It is concluded that HK-1 may participate in neuron-glia interactions both under physiological and inflammatory conditions and mediate pain in the trigeminal system.
Timea Aczél; Angéla Kecskés; József Kun; Kálmán Szenthe; Ferenc Bánáti; Susan Szathmary; Róbert Herczeg; Péter Urbán; Attila Gyenesei; Balázs Gaszner; Zsuzsanna Helyes; Kata Bölcskei. Hemokinin-1 Gene Expression Is Upregulated in Trigeminal Ganglia in an Inflammatory Orofacial Pain Model: Potential Role in Peripheral Sensitization. International Journal of Molecular Sciences 2020, 21, 2938 .
AMA StyleTimea Aczél, Angéla Kecskés, József Kun, Kálmán Szenthe, Ferenc Bánáti, Susan Szathmary, Róbert Herczeg, Péter Urbán, Attila Gyenesei, Balázs Gaszner, Zsuzsanna Helyes, Kata Bölcskei. Hemokinin-1 Gene Expression Is Upregulated in Trigeminal Ganglia in an Inflammatory Orofacial Pain Model: Potential Role in Peripheral Sensitization. International Journal of Molecular Sciences. 2020; 21 (8):2938.
Chicago/Turabian StyleTimea Aczél; Angéla Kecskés; József Kun; Kálmán Szenthe; Ferenc Bánáti; Susan Szathmary; Róbert Herczeg; Péter Urbán; Attila Gyenesei; Balázs Gaszner; Zsuzsanna Helyes; Kata Bölcskei. 2020. "Hemokinin-1 Gene Expression Is Upregulated in Trigeminal Ganglia in an Inflammatory Orofacial Pain Model: Potential Role in Peripheral Sensitization." International Journal of Molecular Sciences 21, no. 8: 2938.
In the last years, the interpretation of gonadotropin-releasing hormone (GnRH) neuropeptide superfamily has changed tremendously. One main driver is the investigation of functions and evolutionary lineage of previously identified molluscan GnRH molecules. Emerging evidence suggests not only reproductive, but also diverse biological effects of these molecules and proposes they should most likely be called corazonin (CRZ). Clearly, a more global understanding necessitates further exploration of species-specific functions and structure of invGnRH/CRZ peptides. Towards this goal, we have identified the full-length cDNA of invGnRH/CRZ peptide in an invertebrate model species, the great pond snail Lymnaea stagnalis, termed ly-GnRH/CRZ, and characterized the transcript and peptide distribution in the central nervous system (CNS) and peripheral organs. Our results are consistent with previous data that molluscan GnRHs are more related to CRZs and serve diverse functions. For this, our findings support the notion that peptides originally termed molluscan GnRH are multifunctional modulators and that nomenclature change should be taken into consideration.
István Fodor; Zita Zrinyi; Péter Urbán; Róbert Herczeg; Gergely Büki; Joris M. Koene; Pei-San Tsai; Zsolt Pirger. Identification, presence, and possible multifunctional regulatory role of invertebrate gonadotropin-releasing hormone/corazonin molecule in the great pond snail (Lymnaea stagnalis). 2020, 1 .
AMA StyleIstván Fodor, Zita Zrinyi, Péter Urbán, Róbert Herczeg, Gergely Büki, Joris M. Koene, Pei-San Tsai, Zsolt Pirger. Identification, presence, and possible multifunctional regulatory role of invertebrate gonadotropin-releasing hormone/corazonin molecule in the great pond snail (Lymnaea stagnalis). . 2020; ():1.
Chicago/Turabian StyleIstván Fodor; Zita Zrinyi; Péter Urbán; Róbert Herczeg; Gergely Büki; Joris M. Koene; Pei-San Tsai; Zsolt Pirger. 2020. "Identification, presence, and possible multifunctional regulatory role of invertebrate gonadotropin-releasing hormone/corazonin molecule in the great pond snail (Lymnaea stagnalis)." , no. : 1.
Medicinal plants are widely used in folk medicine but quite often their composition and biological effects are hardly known. Our study aimed to analyze the composition, cytotoxicity, antimicrobial, antioxidant activity and cellular migration effects of Anthyllis vulneraria, Fuchsia magellanica, Fuchsia triphylla and Lysimachia nummularia used in the Romanian ethnomedicine for wounds. Liquid chromatography with mass spectrometry (LC-MS/MS) was used to analyze 50% (v/v) ethanolic and aqueous extracts of the plants’ leaves. Antimicrobial activities were estimated with a standard microdilution method. The antioxidant properties were evaluated by validated chemical cell-free and biological cell-based assays. Cytotoxic effects were performed on mouse fibroblasts and human keratinocytes with a plate reader-based method assessing intracellular adenosine triphosphate (ATP), nucleic acid and protein contents and also by a flow cytometer-based assay detecting apoptotic–necrotic cell populations. Cell migration to cover cell-free areas was visualized by time-lapse phase-contrast microscopy using standard culture inserts. Fuchsia species showed the strongest cytotoxicity and the highest antioxidant and antimicrobial activity. However, their ethanolic extracts facilitated cell migration, most probably due to their various phenolic acid, flavonoid and anthocyanin derivatives. Our data might serve as a basis for further animal experiments to explore the complex action of Fuchsia species in wound healing assays.
Rita Csepregi; Viktória Temesfői; Sourav Das; Ágnes Alberti; Csenge Anna Tóth; Róbert Herczeg; Nóra Papp; Tamás Kőszegi. Cytotoxic, Antimicrobial, Antioxidant Properties and Effects on Cell Migration of Phenolic Compounds of Selected Transylvanian Medicinal Plants. Antioxidants 2020, 9, 166 .
AMA StyleRita Csepregi, Viktória Temesfői, Sourav Das, Ágnes Alberti, Csenge Anna Tóth, Róbert Herczeg, Nóra Papp, Tamás Kőszegi. Cytotoxic, Antimicrobial, Antioxidant Properties and Effects on Cell Migration of Phenolic Compounds of Selected Transylvanian Medicinal Plants. Antioxidants. 2020; 9 (2):166.
Chicago/Turabian StyleRita Csepregi; Viktória Temesfői; Sourav Das; Ágnes Alberti; Csenge Anna Tóth; Róbert Herczeg; Nóra Papp; Tamás Kőszegi. 2020. "Cytotoxic, Antimicrobial, Antioxidant Properties and Effects on Cell Migration of Phenolic Compounds of Selected Transylvanian Medicinal Plants." Antioxidants 9, no. 2: 166.