Ultraviolet (UV) radiation is a well-implemented process for water disinfection. The development of emergent UV sources, such as light-emitting diodes (LEDs), has afforded new possibilities for advanced oxidation processes. The emission wavelength is considered to be an important factor for photo-chemical processes in terms of both biological damage and energetic efficiency, as the inactivation mechanisms and mode-of-action may differ according to the wavelength that is applied. In addition, these processes merit exploration for inactivating emerging pathogens, such as marine vibrios, that are important bacteria to control in maritime activities. The main goal of this study was to compare the disinfection efficacy of several UV-LED driven processes with different modes of action. First, the effect of UV-LEDs was assessed at different UV ranges (UV-A, UV-B, or UV-C). Second, the possible enhancement of a combination with hydrogen peroxide (H₂O₂) or peroxymonosulfate salt (HSO₅^-) was investigated under two different application strategies, i.e. simultaneous or sequential. The results obtained indicate a high sensitivity of Vibrio alginolyticus to UV radiation, especially under UV-B (k_obs = 0.24 cm²/mJ) and UV-C (k_obs = 1.47 cm²/mJ) irradiation. The highest inactivation rate constants were obtained for UV/HSO₅^- (k_obs (cm²/mJ)=0.0007 (UV-A); 0.39 (UV-B); 1.79 (UV-C)) with respect to UV/H₂O₂ (k_obs (cm²/mJ)=0.0006 (UV-A); 0.26 (UV-B); and 1.54 (UV-C)) processes, however, regrowth was avoided only with UV/H₂O₂. Additionally, the disinfection enhancement caused by a chemical addition was more evident in the order UV-A > UV-B > UV-C. By applying H₂O₂ (10 mg/L) or HSO₅^- (2.5 mg/L) in a sequential mode before the UV, negligible effects were obtained in comparison with the simultaneous application. Finally, promising electrical energy per order (EE_O) values were obtained as follows: UV/HSO₅^- (EE_O (kWh/m³)=1.68 (UV-A); 0.20 (UV-B); 0.04 (UV-C)) and UV/H₂O₂ (EE_O (kWh/m³)=2.15 (UV-A); 0.32 (UV-B); 0.04 (UV-C)), demonstrating the potential of UV-LEDs for disinfection in particular activities such as the aquaculture industry or maritime transport.

Due to the increasing number of ecosystem invasions with the introduction of exogenous species via ballast water, the International Maritime Organization adopted the Ballast Water Convention (BWMC). The BWMC establishes standards for the concentration of viable organisms in a ballast water discharge. Ultraviolet (UV) irradiation is commonly used for treating ballast water; however, regrowth after UV irradiation and other drawbacks have been reported. In this study, improvement in UV treatment with the addition of hydrogen peroxide or peroxymonosulfate salt was investigated using the microalgae Tetraselmis suecica as the target organism. Results reported that each of these reagents added in a concentration of 10 ppm reduced the concentration of initial cells by more than 96%, increased the UV inactivation rate, and enabled reaching greater level of inactivation with the treatment. These improvements imply a reduction of the UV doses required for a consistent compliance with the BWMC standards.