The detection of metabolites is very important for the estimation of the health of human beings. Latent fingerprint contains many constituents and specific contaminants, which give much information of the individual, such as health status, drug abuse etc. For a long time, many efforts have been focused on visualizing latent fingerprints, but little attention has been paid to the detection of such substances at the same time. In this article, we have devised a versatile approach for the ultra-sensitive detection and identification of specific biomolecules deposited within fingerprints via a large-area SERS imaging technique. The antibody bound to the Raman probe modified silver nanoparticles enables the binding to specific proteins within the fingerprints to afford high-definition SERS images of the fingerprint pattern. The SERS spectra and images of Raman probes indirectly provide chemical information regarding the given proteins. By taking advantage of the high sensitivity and the capability of SERS technique to obtain abundant vibrational signatures of biomolecules, we have successfully detected minute quantities of protein present within a latent fingerprint. This technique provides a versatile and effective model to detect biomarkers within fingerprints for medical diagnostics, criminal investigation and other fields.

The effect of the ZrO₂ crystal form on surface-enhanced Raman scattering (SERS) activity was studied. The ratio of the tetragonal (T) and monoclinic (M) phases of ZrO₂ nanoparticles (ZrO₂ NPs) was controlled by regulating the ratio of two types of additives in the hydrothermal synthesis method. The SERS intensity of 4-mercaptobenzoic acid (4–MBA) was gradually enhanced by changing the M and T phase ratio in ZrO₂ NPs. The degree of charge transfer (CT) in the enhanced 4–MBA molecule was greater than 0.5, indicating that CT was the main contributor to SERS. The intensity of SERS was strongest when the ratio of the T crystal phase in ZrO₂ was 99.7%, and the enhancement factor reached 2.21 × 10⁴. More importantly, the proposed study indicated that the T and M phases of the ZrO₂ NPs affected the SERS enhancement. This study provides a new approach for developing high-quality SERS substrates and improving the transmission efficiency of molecular sensors.

In this study, ZrO₂ and Zn–ZrO₂ nanoparticles (NPs) with a series of Zn ion doping amounts were synthesized by the sol-gel process and utilized as substrates for surface-enhanced Raman scattering (SERS). After absorbing the probing molecule 4–mercaptobenzoic acid, the SERS signal intensities of Zn–ZrO₂ NPs were all greater than that of the pure ZrO₂. The 1% Zn doping concentration ZrO₂ NPs exhibited the highest SERS enhancement, with an enhancement factor (EF) value of up to 10⁴. X-ray diffraction, X-ray photoelectron spectroscopy, Ultraviolet (UV) photoelectron spectrometer, UV–vis spectroscopy, Transmission Electron Microscope (TEM), and Raman spectroscopy were used to characterize the properties of Zn–ZrO₂ NPs and explore the mechanisms behind the SERS phenomenon. The charge transfer (CT) process is considered to be responsible for the SERS performance of 4–MBA adsorbed on Zn–ZrO₂. The results of this study demonstrate that an appropriate doping ratio of Zn ions can promote the charge transfer process between ZrO₂ NPs and probe molecules and significantly improve the SERS properties of ZrO₂ substrates.