Soil salinization and water shortage cause ion imbalance and hyperosmoticity in plant cells, adversely impairing photosynthesis efficiency. How soil salinity-induced photosynthetic acclimation influences the cross-tolerance to drought conditions represents a promising research topic. This study was to reveal the photosynthetic mechanism of soil salinity-induced resistance to the subsequent drought stress in tomato leaves through comprehensive photosynthesis-related spectroscopy analysis. We conducted soil salinity pretreatment and subsequent drought stress experiments, including irrigation with 100 mL water, 100 mL 100 mM NaCl solution (NaCl100), 50 mL water, and 50 mL 100 mM NaCl solution (NaCl50) for five days, followed by five-day drought stress. The results showed that soil salinity treatment by NaCl decreased the rate of photosynthetic gas exchange but enhanced CO₂ assimilation, along with photosystem II [PS(II)] and photosystem I [PS(I)] activity and photochemical efficiency in tomato plants compared with water pretreatment after subsequent drought stress. NaCl100 and NaCl50 had the capacity to maintain non-photochemical quenching (NPQ) of chlorophyll fluorescence and the cyclic electron (CEF) flow around PSI in tomato leaves after being subjected to subsequent drought stress, thus avoiding the decrease of photosynthetic efficiency under drought conditions. NaCl100 and NaCl50 pretreatment induced a higher proton motive force (pmf) and also alleviated the damage to the thylakoid membrane and adenosine triphosphate (ATP) synthase of tomato leaves caused by subsequent drought stress. Overall, soil salinity treatment could enhance drought resistance in tomato plants by inducing NPQ, maintaining CEF and pmf that tradeoff between photoprotection and photochemistry reactions. This study also provides a photosynthetic perspective for salt and drought cross-tolerance.

Plants often need to withstand multiple types of environmental stresses (e.g., salt and low temperature stress) because of their sessile nature. Although the physiological responses of plants to single stressor have been well-characterized, few studies have evaluated the extent to which pretreatment with non-lethal stressors can maintain the photosynthetic performance of plants in adverse environments (i.e., acclimation-induced cross-tolerance). Here, we studied the effects of sodium chloride (NaCl) pretreatment on the photosynthetic performance of tomato plants exposed to low temperature stress by measuring photosynthetic and chlorophyll fluorescence parameters, stomatal aperture, chloroplast quality, and the expression of stress signaling pathway-related genes. NaCl pretreatment significantly reduced the carbon dioxide assimilation rate, transpiration rate, and stomatal aperture of tomato leaves, but these physiological acclimations could mitigate the adverse effects of subsequent low temperatures compared with non-pretreated tomato plants. The content of photosynthetic pigments decreased and the ultra-microstructure of chloroplasts was damaged under low temperature stress, and the magnitude of these adverse effects was alleviated by NaCl pretreatment. The quantum yield of photosystem I (PSI) and photosystem II (PSII), the quantum yield of regulatory energy dissipation, and non-photochemical energy dissipation owing to donor-side limitation decreased following NaCl treatment; however, the opposite patterns were observed when NaCl-pretreated plants were exposed to low temperature stress. Similar results were obtained for the electron transfer rate of PSI, the electron transfer rate of PSII, and the estimated cyclic electron flow value (CEF). The production of reactive oxygen species induced by low temperature stress was also significantly alleviated by NaCl pretreatment. The expression of ion channel and tubulin-related genes affecting stomatal aperture, chlorophyll synthesis genes, antioxidant enzyme-related genes, and abscisic acid (ABA) and low temperature signaling-related genes was up-regulated in NaCl-pretreated plants under low temperature stress. Our findings indicated that CEF-mediated photoprotection, stomatal movement, the maintenance of chloroplast quality, and ABA and low temperature signaling pathways all play key roles in maintaining the photosynthetic capacity of NaCl-treated tomato plants under low temperature stress.

Large-scale high throughput metabolomic technologies are indispensable components of systems biology in terms of discovering and defining the metabolite parts of the system. However, the lack of a plant metabolite spectral library limits the metabolite identification of plant metabolomic studies. Here, we have created a plant metabolite spectral library using 544 authentic standards, which increased the efficiency of identification for untargeted metabolomic studies. The process of creating the spectral library was described, and the mzVault library was deposited in the public repository for free download. Furthermore, based on the spectral library, we describe a process of creating a pseudo-targeted method, which was applied to a proof-of-concept study of Arabidopsis leaf extracts. As authentic standards become available, more metabolite spectra can be easily incorporated into the spectral library to improve the mzVault package.