The red flour beetle, Tribolium castaneum, is one of the most common and economically important pests of stored cereal products worldwide. Furthermore, these beetles can act as vectors for several fungal post-harvest diseases. In this study, we collected T. castaneum from 49 rice processing complexes (RPCs) nationwide during 2016-2017 and identified contaminating fungal species on the surface of the beetles. Five beetles from each region were placed on potato dextrose agar media or Fusarium selection media after wet processing with 100% relative humidity at 27°C for one week. A total of 142 fungal isolates were thus collected. By sequence analysis of the internal transcribed spacer region, 23 fungal genera including one unidentified taxon were found to be associated with T. castaneum. The genus Aspergillus spp. (28.9%) was the most frequently present, followed by Cladosporium spp. (12.0%), Hyphopichia burtonii (9.2%), Penicillium spp. (8.5%), Mucor spp. (6.3%), Rhizopus spp. (5.6%), Cephaliophora spp. (3.5%), Alternaria alternata (2.8%) and Monascus sp. (2.8%). Less commonly identified were genera Fusarium, Nigrospora, Beauveria, Chaetomium, Coprinellus, Irpex, Lichtheimia, Trichoderma, Byssochlamys, Cochliobolus, Cunninghamella, Mortierella, Polyporales, Rhizomucor and Talaromyces. Among the isolates, two known mycotoxin-producing fungi, Aspergillus flavus and Fusarium spp. were also identified. This result is consistent with previous studies that surveyed fungal and mycotoxin contamination in rice from RPCs. Our study indicates that the storage pest, T. castaneum, would play an important role in spreading fungal contaminants and consequently increasing mycotoxin contamination in stored rice.

Mealybugs found on grapes during quarantine in Korea are fumigated with methyl bromide (MB). However, MB is an ozone-depleting, highly toxic pesticide; therefore, the International Plant Protection Convention, a representative organization involved in quarantine, recommends using MB alternatives. We evaluated the feasibility of using liquid ethyl formate (EF), a new EF formulation, to control mealybugs (Pseudococcus comstocki) on grapes (Campbell Early). Large-scale tests and the comparative evaluation of EF and MB desorption from grapes were conducted during the simulated 72-h post-fumigation period. Dose–response tests showed that the EF concentration and time product causing 99% mortality (LCt₉₉) at 5 °C was 47.36 and 145.85 g h/m³ for adults and eggs, respectively. EF treatment at 70 g/m³ for 4 h at 5 °C with a loading ratio of 10% achieved an LCt₉₉ of 145.85 g h/m³ on P. comstocki, confirming EF efficacy on mealybugs without phytotoxic effects on grapes. EF fumigation may also be safer because EF concentrations were maintained at less than 100 ppm, the specified exposure limit of EF; meanwhile, those of MB were higher than the exposure limit (1 ppm). Therefore, liquid EF can be used as a safer alternative to MB in phytosanitary treatments of grapes to control P. comstocki.

We sequenced the complete mitochondrial genome of Aclees taiwanensis collected in Korea. The circular mitogenome of A. taiwanensis is 17,435 bp, longer than that of Aclees cribratus, and includes 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNAs, and a control region/D-loop. The AT ratio is 75.4%. Maximum-likelihood and Bayesian inference phylogenetic trees showed that A. taiwanensis was clustered with A. cribratus with full-support values for both trees.

Sweet persimmons are a valuable export commodity. However, the presence of live insects such as Asiacornococcus kaki limits their access to many export markets. Methyl bromide, traditionally used for pest control, is damaging to human health and the environment. Ethyl formate (EF) is a viable alternative; however, its effectiveness against A. kaki on sweet persimmon fruit is unknown. We evaluated the effectiveness of EF fumigation in controlling A. kaki present under the calyx of persimmon fruit. The hatching rate of eggs and the survival rates of nymphs and adults of A. kaki at low temperatures, its LCt₅₀ and LCt₉₉ after EF exposure, and phytotoxic damage caused by EF were evaluated in laboratory-scale and commercial-scale tests. The dose–response tests showed that the EF LCt₉₉ at 5 °C was 9.69, 42.13, and 126.13 g h m⁻³ for adults, nymphs, and eggs, respectively. Commercial-scale tests demonstrated EF efficacy against all A. kaki stages without causing phytotoxic effects on persimmons, though the eggs of A. kaki were not completely controlled in linear low-density polyethylene (LLDPE)-packaged fruit. This study demonstrated that EF is a potential fumigant for quarantine pretreatment, especially before persimmon fruit is packed with LLDPE film, to control A. kaki infesting sweet persimmon fruit.

Pine wilt disease in Korea can be managed by reducing vector density through chemical application. To reduce the side effects from pesticides, effective natural enemies must be identified and used to reduce the vectors’ natural density. Sentinel logs were used to investigate a parasitoid wasp parasitic to Monochamus alternatus and Monochamus saltuarius, the vectors of Bursaphelenchus xylophilus, which causes this disease. During 2016–2017, the parasitoid wasps distributed in the Pinus densiflora and Pinus koraiensis forests were surveyed using sentinel logs at six different areas. An ectoparasitoid wasp Spathius verustus Chao of M. alternatus and M. saltuarius was identified. We showed for the first time that S. verustus was parasitic to M. alternatus in South Korea and to M. saltuarius worldwide. The parasitism rates were affected by region and session but not by the sentinel log height in the tree and the distances between the trap and forest edge locations. Studies also showed that S. verustus appeared to prefer M. alternatus to M. saltuarius as a host. This study unveiled the ecological details of S. verustus. Further research on various environmental factors such as regional differences, host density differences, and the degree of damage from the pine wilt disease is required to understand the effects of environmental or ecological factors on parasitism rates.