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The total lipid content and lipidic profile of seaweeds harvested in the North Coast and purchased in Portugal were determined in this paper. The amount of total lipids in the different species of seaweeds varied between 0.7 ± 0.1% (Chondrus crispus) and 3.8 ± 0.6% (Ulva spp.). Regarding the fatty acid content, polyunsaturated fatty acids (PUFA) ranged between 0–35%, with Ulva spp. presenting the highest amount; monounsaturated fatty acids (MUFA) varied between 19 and 67%; and saturated fatty acids (SFA) were predominant in C. crispus (45–78%) and Gracilaria spp. (36–79%). Concerning the nutritional indices, the atherogenicity index (AI) was between 0.4–3.2, the thrombogenicity index (TI) ranged from 0.04 to 1.95, except for Gracilaria spp., which had a TI of 7.6, and the hypocholesterolemic/hypercholesterolemic ratio (HH) values ranged between 0.88–4.21, except for Gracilaria spp., which exhibited values between 0.22–9.26. The n6/n3 ratio was below 1 for most of the species evaluated, except for Ascophyllum nodosum, which presented a higher value, although below 2. Considering the PUFA/SFA ratio, seaweeds presented values between 0.11–1.02. The polycyclic aromatic hydrocarbons (PAHs) and aliphatic hydrocarbons (AHCs) contamination of seaweeds under study was also quantified, the values found being much lower than the maximum levels recommended for foodstuff.
Cristina Soares; Sara Sousa; Susana Machado; Elsa Vieira; Ana Carvalho; Maria Ramalhosa; Simone Morais; Manuela Correia; Teresa Oliva-Teles; Valentina Domingues; Cristina Delerue-Matos. Bioactive Lipids of Seaweeds from the Portuguese North Coast: Health Benefits versus Potential Contamination. Foods 2021, 10, 1366 .
AMA StyleCristina Soares, Sara Sousa, Susana Machado, Elsa Vieira, Ana Carvalho, Maria Ramalhosa, Simone Morais, Manuela Correia, Teresa Oliva-Teles, Valentina Domingues, Cristina Delerue-Matos. Bioactive Lipids of Seaweeds from the Portuguese North Coast: Health Benefits versus Potential Contamination. Foods. 2021; 10 (6):1366.
Chicago/Turabian StyleCristina Soares; Sara Sousa; Susana Machado; Elsa Vieira; Ana Carvalho; Maria Ramalhosa; Simone Morais; Manuela Correia; Teresa Oliva-Teles; Valentina Domingues; Cristina Delerue-Matos. 2021. "Bioactive Lipids of Seaweeds from the Portuguese North Coast: Health Benefits versus Potential Contamination." Foods 10, no. 6: 1366.
The present work aimed at studying Saccorhiza polyschides extracts obtained by subcritical water extraction as a potential source of essential macro and trace elements, aiming for its potential application as a biofertilizer. The mineral composition, as well as sulfate, chlorine and iodine, total organic matter, and total nitrogen content, were determined on the extracts obtained from seaweeds harvested during low tide at the northern Portuguese coast. The selected parameters are important for a biofertilizer. Among the macronutrients, the most abundant was K (15.7 ± 0.2 g/L), followed by Na (5.46 ± 0.11 g/L), S (1.52 ± 0.06 g/L), Ca (1.09 ± 0.11 g/L), and Mg (1.02 ± 0.08 g/L). Several important micronutrients (Zn, B, Cl, P, Mo, V, Se, and I) have also been found in the extracts. The total organic matter was 34.1 ± 0.3 g/L. The extracts present low levels of toxic compounds such as Ni, Cd, and Pb. Considering the composition of the obtained extracts, these can find application in the development of fertilization products. The composition of subcritical water extracts of S. polyschides suggests that they may have important characteristics as a biofertilizer and can be an option in biofortification experiments with essential nutrients. The method can be easily scaled up which makes it attractive for agricultural applications.
Cristina Soares; Jaroslava Švarc-Gajić; Maria Teresa Oliva-Teles; Edgar Pinto; Nataša Nastić; Saša Savić; Agostinho Almeida; Cristina Delerue-Matos. Mineral Composition of Subcritical Water Extracts of Saccorhiza Polyschides, a Brown Seaweed Used as Fertilizer in the North of Portugal. Journal of Marine Science and Engineering 2020, 8, 244 .
AMA StyleCristina Soares, Jaroslava Švarc-Gajić, Maria Teresa Oliva-Teles, Edgar Pinto, Nataša Nastić, Saša Savić, Agostinho Almeida, Cristina Delerue-Matos. Mineral Composition of Subcritical Water Extracts of Saccorhiza Polyschides, a Brown Seaweed Used as Fertilizer in the North of Portugal. Journal of Marine Science and Engineering. 2020; 8 (4):244.
Chicago/Turabian StyleCristina Soares; Jaroslava Švarc-Gajić; Maria Teresa Oliva-Teles; Edgar Pinto; Nataša Nastić; Saša Savić; Agostinho Almeida; Cristina Delerue-Matos. 2020. "Mineral Composition of Subcritical Water Extracts of Saccorhiza Polyschides, a Brown Seaweed Used as Fertilizer in the North of Portugal." Journal of Marine Science and Engineering 8, no. 4: 244.
This study aimed to develop new canned chub mackerel products incorporating edible seaweeds (Ascophyllum nodosum, Fucus spiralis, Saccorhiza polyschides, Chondrus crispus, Porphyra sp. and Ulva sp.) harvested in the Portuguese North-Central coast, with simultaneous sensory improvement and minerals enrichment. Two processes were compared, namely the addition of seaweeds in i) the canning step and ii) in the brining step (as the replacement for salt). The concentrations of four macrominerals (Na, K, Ca and Mg), chloride, and twelve trace elements (Co, Cu, Fe, I, Li, Mn, Mo, Rb, Se, Sr, V and Zn) were determined by high-resolution continuum source flame atomic absorption spectrometry (HR-CS-FAAS) and inductively coupled plasma mass spectrometry (ICP-MS), respectively. Results showed that canned chub mackerel incorporating C. crispus and F. spiralis was found to be the preferred sensory option, also exhibiting contents enriched with Cl, Co, Cu, Fe, I, Li, Mg, Mn, Mo, Na, Rb, Se, and Sr. This effect was more pronounced when both seaweed species were added to replace the salt added in the brining step.
Elsa F. Vieira; Cristina Soares; Susana Machado; M. Teresa Oliva-Teles; Manuela Correia; Maria João Ramalhosa; Ana Carvalho; Valentina F. Domingues; Filipa Antunes; Simone Morais; Cristina Delerue-Matos. Development of New Canned Chub Mackerel Products Incorporating Edible Seaweeds—Influence on the Minerals and Trace Elements Composition. Molecules 2020, 25, 1133 .
AMA StyleElsa F. Vieira, Cristina Soares, Susana Machado, M. Teresa Oliva-Teles, Manuela Correia, Maria João Ramalhosa, Ana Carvalho, Valentina F. Domingues, Filipa Antunes, Simone Morais, Cristina Delerue-Matos. Development of New Canned Chub Mackerel Products Incorporating Edible Seaweeds—Influence on the Minerals and Trace Elements Composition. Molecules. 2020; 25 (5):1133.
Chicago/Turabian StyleElsa F. Vieira; Cristina Soares; Susana Machado; M. Teresa Oliva-Teles; Manuela Correia; Maria João Ramalhosa; Ana Carvalho; Valentina F. Domingues; Filipa Antunes; Simone Morais; Cristina Delerue-Matos. 2020. "Development of New Canned Chub Mackerel Products Incorporating Edible Seaweeds—Influence on the Minerals and Trace Elements Composition." Molecules 25, no. 5: 1133.
Oilseeds from five native plant species with edible potential from the Brazilian Caatinga semi-arid region (Diplopterys pubipetala, Barnebya harleyi, Croton adamantinus, Hippocratea volubilis, and Couroupita guianensis) were investigated regarding their mineral contents. The minerals, Na, K, Ca, Mg, Fe, Cu, Cr, Al, were analyzed by high-resolution continuum source atomic absorption spectrometry (HR–CS AAS) and P by the vanadomolybdophosphoric acid colorimetric method. K, Mg, and P were the main elements found (1.62–3.7 mg/g, 362–586 µg/g, and 224–499 µg/g dry weight (dw), respectively). B. harley seeds contained the highest amounts of K and P, while C. guianensis seeds were the richest in Mg. Fe was the most abundant oligoelement (2.3–25.6 µg/g dw). Cr contents were below the limit of quantification for all samples and Al amounts were low: 0.04–1.80 µg/g dw. A linear discriminant analysis clearly differentiated B. harleyi and C. guianensis samples from the remaining ones. In sum, these oilseeds from the Brazilian Caatinga semi-arid region seem to have the potential to be used as natural sources of minerals, mainly K.
Ivone M. C. Almeida; M. Teresa Oliva-Teles; Rita C. Alves; Joana Santos; Roberta S. Pinho; Suzene I. Silva; Cristina Delerue-Matos; M. Beatriz P. P. Oliveira. Oilseeds from a Brazilian Semi-Arid Region: Edible Potential Regarding the Mineral Composition. Foods 2020, 9, 229 .
AMA StyleIvone M. C. Almeida, M. Teresa Oliva-Teles, Rita C. Alves, Joana Santos, Roberta S. Pinho, Suzene I. Silva, Cristina Delerue-Matos, M. Beatriz P. P. Oliveira. Oilseeds from a Brazilian Semi-Arid Region: Edible Potential Regarding the Mineral Composition. Foods. 2020; 9 (2):229.
Chicago/Turabian StyleIvone M. C. Almeida; M. Teresa Oliva-Teles; Rita C. Alves; Joana Santos; Roberta S. Pinho; Suzene I. Silva; Cristina Delerue-Matos; M. Beatriz P. P. Oliveira. 2020. "Oilseeds from a Brazilian Semi-Arid Region: Edible Potential Regarding the Mineral Composition." Foods 9, no. 2: 229.
The total protein content and the (total and free) amino acid composition of nine edible species of red, brown and green seaweeds collected in the Portuguese North-Central coast were quantified to assess their potential contribution to the recommended dietary intake. Whenever possible, the protein and amino acid composition was compared with that of commercial European seaweeds. The protein content was the highest (P<0.05) in red species (19.1-28.2 g/100g dw), followed by the green seaweed Ulva spp. (20.5-23.3 g/100g dw), with the lowest content found in brown seaweeds (6.90-19.5 g/100g dw). Brown seaweeds presented the lowest mean contents of essential amino acids (EAAs) (41.0% protein) but significantly (P<0.05) higher concentrations of non-essential amino acids (36.1% protein) and free amino acids (6.47-24.0% protein). Tryptophan, methionine and leucine were the limiting EAAs in all species. In contrast, lysine was found in high concentrations, especially in red (2.71-3.85% protein) and green (2.84-4.24% protein) seaweeds.
Elsa Ferreira Vieira; Cristina Soares; Susana Machado; Manuela Correia; Maria João Ramalhosa; Maria Teresa Oliva-Teles; Ana Carvalho; Valentina Domingues; Filipa Antunes; Teresa Azevedo Cardoso Oliveira; Simone Morais; Cristina Delerue-Matos. Seaweeds from the Portuguese coast as a source of proteinaceous material: Total and free amino acid composition profile. Food Chemistry 2018, 269, 264 -275.
AMA StyleElsa Ferreira Vieira, Cristina Soares, Susana Machado, Manuela Correia, Maria João Ramalhosa, Maria Teresa Oliva-Teles, Ana Carvalho, Valentina Domingues, Filipa Antunes, Teresa Azevedo Cardoso Oliveira, Simone Morais, Cristina Delerue-Matos. Seaweeds from the Portuguese coast as a source of proteinaceous material: Total and free amino acid composition profile. Food Chemistry. 2018; 269 ():264-275.
Chicago/Turabian StyleElsa Ferreira Vieira; Cristina Soares; Susana Machado; Manuela Correia; Maria João Ramalhosa; Maria Teresa Oliva-Teles; Ana Carvalho; Valentina Domingues; Filipa Antunes; Teresa Azevedo Cardoso Oliveira; Simone Morais; Cristina Delerue-Matos. 2018. "Seaweeds from the Portuguese coast as a source of proteinaceous material: Total and free amino acid composition profile." Food Chemistry 269, no. : 264-275.
Limited information exists on the bioaccessibility and intestinal uptake of essential minerals from different types of beans cooked through different cooking methods. This study aimed to estimate the in vitro bioaccessibility and intestinal uptake of the essential minerals K, Ca, Mg, Fe, Zn, Mn, Cu and Mo from four different types of beans (kidney, black, white and cowpea) cooked under different conditions (home-cooked and industrially processed canned product). Results showed that the bioaccessibility and uptake of most essential minerals is higher in canned beans. Mn was the mineral that presented the highest bioaccessibility (43–63%), and Mo had the lowest (3–36%). The highest uptake was observed for Mg (59.9%), while the lowest was found for Fe (10.5%). Regarding the type of beans, white beans presented the highest bioaccessibility and uptake for all the analyzed essential minerals and thus its consumption is preferable from a nutritional point of view.
Miguel A. Faria; Alfredo Araújo; Edgar Pinto; César Oliveira; M. Teresa Oliva-Teles; Agostinho Almeida; Cristina Delerue-Matos; Isabel M.P.L.V.O. Ferreira. Bioaccessibility and intestinal uptake of minerals from different types of home-cooked and ready-to-eat beans. Journal of Functional Foods 2018, 50, 201 -209.
AMA StyleMiguel A. Faria, Alfredo Araújo, Edgar Pinto, César Oliveira, M. Teresa Oliva-Teles, Agostinho Almeida, Cristina Delerue-Matos, Isabel M.P.L.V.O. Ferreira. Bioaccessibility and intestinal uptake of minerals from different types of home-cooked and ready-to-eat beans. Journal of Functional Foods. 2018; 50 ():201-209.
Chicago/Turabian StyleMiguel A. Faria; Alfredo Araújo; Edgar Pinto; César Oliveira; M. Teresa Oliva-Teles; Agostinho Almeida; Cristina Delerue-Matos; Isabel M.P.L.V.O. Ferreira. 2018. "Bioaccessibility and intestinal uptake of minerals from different types of home-cooked and ready-to-eat beans." Journal of Functional Foods 50, no. : 201-209.
Polymeric ion selective electrodes are highly sensitive to changes in zero current ion flow and this offers a route to signal amplification in label-free potentiometric immunosensors. In this work, a label-free potentiometric immunosensor toward Salmonella typhimurium (ST) assembled in a home-made pipette-tip electrode is described. The signal-output amplification was implemented on a gold nanoparticle polymer inclusion membrane (AuNPs-PIM) which was used as sensing platform and for antibody immobilization. Additionally, a marker ion was used to detect the antibody-antigen binding event at the electrode surface. The immunosensor construction was performed in several steps: i) gold salt ions extraction in PVC membrane; ii) AuNPs formation using Na2EDTA as reduction agent; iii) antibody anti-Salmonella conjugation on AuNPs-PIM in pipette-tip electrodes. The potential shift observed in potentiometric measurements was derived simply from the blocking effect in the ionic flux caused by antigen-antibody conjugation, without no extra steps, mimetizing the ion-channel sensors. A detection limit of 6 cells mL-1 was attained. As proof-of-concept, recovery studies were performed in spiked commercial apple juice samples with success. Due to the simplicity of use, the appealing cost of equipment and sensor production and being able to provide a quick analytical response (less than 1 h for a complete assay, including sample preparation for analysis), this scheme represents a good prototype device for the detection of foodborne pathogens like ST or other immune-responsive bacteria.
Nádia Silva; Júlia M.C.S. Magalhães; M. Fátima Barroso; Maria Teresa Oliva-Teles; Cristina Freire; Cristina Delerue-Matos. In situ formation of gold nanoparticles in polymer inclusion membrane: Application as platform in a label-free potentiometric immunosensor for Salmonella typhimurium detection. Talanta 2018, 194, 134 -142.
AMA StyleNádia Silva, Júlia M.C.S. Magalhães, M. Fátima Barroso, Maria Teresa Oliva-Teles, Cristina Freire, Cristina Delerue-Matos. In situ formation of gold nanoparticles in polymer inclusion membrane: Application as platform in a label-free potentiometric immunosensor for Salmonella typhimurium detection. Talanta. 2018; 194 ():134-142.
Chicago/Turabian StyleNádia Silva; Júlia M.C.S. Magalhães; M. Fátima Barroso; Maria Teresa Oliva-Teles; Cristina Freire; Cristina Delerue-Matos. 2018. "In situ formation of gold nanoparticles in polymer inclusion membrane: Application as platform in a label-free potentiometric immunosensor for Salmonella typhimurium detection." Talanta 194, no. : 134-142.
Mucosal T lymphocytes from patients with ulcerative colitis (UC) were previously shown to display a deficiency in branched N-glycosylation associated with disease severity. However, whether this glycosylation pathway shapes the course of the T cell response constituting a targeted-specific mechanism in UC remains largely unknown. In this study, we demonstrated that metabolic supplementation of ex vivo mucosal T cells from patients with active UC with N-acetylglucosamine (GlcNAc) resulted in enhancement of branched N-glycosylation in the T cell receptor (TCR), leading to suppression of T cell growth, inhibition of the T helper 1 (Th1)/Th17 immune response, and controlled T cell activity. We further demonstrated that mouse models displaying a deficiency in the branched N-glycosylation pathway (MGAT5 −/−, MGAT5 +/−) exhibited increased susceptibility to severe forms of colitis and early-onset disease. Importantly, the treatment of these mice with GlcNAc reduced disease severity and suppressed disease progression due to a controlled T cell-mediated immune response at the intestinal mucosa. In conclusion, our human ex vivo and preclinical results demonstrate the targeted-specific immunomodulatory properties of this simple glycan, proposing a therapeutic approach for patients with UC.
Ana Dias; Alexandra Correia; Márcia Pereira; Catarina R. Almeida; Inês Alves; Vanda Pinto; Telmo A. Catarino; Nuno Mendes; Magdalena Leander; Maria Teresa Oliva-Teles; Luís Maia; Cristina Delerue-Matos; Naoyuki Taniguchi; Margarida Lima; Isabel Pedroto; Ricardo Marcos-Pinto; Paula Lago; Celso A. Reis; Manuel Vilanova; Salomé S. Pinho. Metabolic control of T cell immune response through glycans in inflammatory bowel disease. Proceedings of the National Academy of Sciences 2018, 115, E4651 -E4660.
AMA StyleAna Dias, Alexandra Correia, Márcia Pereira, Catarina R. Almeida, Inês Alves, Vanda Pinto, Telmo A. Catarino, Nuno Mendes, Magdalena Leander, Maria Teresa Oliva-Teles, Luís Maia, Cristina Delerue-Matos, Naoyuki Taniguchi, Margarida Lima, Isabel Pedroto, Ricardo Marcos-Pinto, Paula Lago, Celso A. Reis, Manuel Vilanova, Salomé S. Pinho. Metabolic control of T cell immune response through glycans in inflammatory bowel disease. Proceedings of the National Academy of Sciences. 2018; 115 (20):E4651-E4660.
Chicago/Turabian StyleAna Dias; Alexandra Correia; Márcia Pereira; Catarina R. Almeida; Inês Alves; Vanda Pinto; Telmo A. Catarino; Nuno Mendes; Magdalena Leander; Maria Teresa Oliva-Teles; Luís Maia; Cristina Delerue-Matos; Naoyuki Taniguchi; Margarida Lima; Isabel Pedroto; Ricardo Marcos-Pinto; Paula Lago; Celso A. Reis; Manuel Vilanova; Salomé S. Pinho. 2018. "Metabolic control of T cell immune response through glycans in inflammatory bowel disease." Proceedings of the National Academy of Sciences 115, no. 20: E4651-E4660.
The Portuguese coast presents a large amount of potentially edible seaweeds that are underexploited. The identification of different macroalgae species and their availability in the northern and central coast of the continental territory was assessed. The nutritional value of seaweeds is discussed based on a literature review (when available) focused on data for species collected in Portugal with the aim to define the most important nutritional parameters that should be characterized in the samples. Possible health concerns related with the presence of contaminants are also considered.
C. Soares; S. Machado; E.F. Vieira; S. Morais; M.T. Teles; M. Correia; A. Carvalho; V. F. Domingues; M.J. Ramalhosa; C. Delerue-Matos; F. Antunes. Seaweeds from the Portuguese coast: A potential food resource? IOP Conference Series: Materials Science and Engineering 2017, 231, 012126 .
AMA StyleC. Soares, S. Machado, E.F. Vieira, S. Morais, M.T. Teles, M. Correia, A. Carvalho, V. F. Domingues, M.J. Ramalhosa, C. Delerue-Matos, F. Antunes. Seaweeds from the Portuguese coast: A potential food resource? IOP Conference Series: Materials Science and Engineering. 2017; 231 (1):012126.
Chicago/Turabian StyleC. Soares; S. Machado; E.F. Vieira; S. Morais; M.T. Teles; M. Correia; A. Carvalho; V. F. Domingues; M.J. Ramalhosa; C. Delerue-Matos; F. Antunes. 2017. "Seaweeds from the Portuguese coast: A potential food resource?" IOP Conference Series: Materials Science and Engineering 231, no. 1: 012126.
Nine plant infusions were assessed in terms of their polyphenolic compounds and antioxidant capacity properties by performing a “battery” of assays. Bioactive compounds (total phenolic and flavonoid content) were measured using spectrophotometric procedures and the total antioxidant capacity was determined by DPPH●-RSA, FRAP and DNA-based biosensor against the reactive oxygen species: HO● and H2O2. Although the optical methods are based on the donation of hydrogen or electrons between antioxidants and radicals, the DNA-based biosensor intends to simulate an in vivo assay by exposing DNA layer to biological ROS and antioxidants. In general, green and black tea showed higher polyphenolic content and antioxidant capacity evaluated by the described methods. All the analysed plant beverages showed to be a good source of bioactive compounds, particularly the green and black tea, peppermint and senna.
M. Fátima Barroso; Maria João Ramalhosa; Rita C. Alves; Alexandra Dias; Cristina M.D. Soares; Maria Teresa Oliva-Teles; Cristina Delerue-Matos. Total antioxidant capacity of plant infusions: Assessment using electrochemical DNA-based biosensor and spectrophotometric methods. Food Control 2016, 68, 153 -161.
AMA StyleM. Fátima Barroso, Maria João Ramalhosa, Rita C. Alves, Alexandra Dias, Cristina M.D. Soares, Maria Teresa Oliva-Teles, Cristina Delerue-Matos. Total antioxidant capacity of plant infusions: Assessment using electrochemical DNA-based biosensor and spectrophotometric methods. Food Control. 2016; 68 ():153-161.
Chicago/Turabian StyleM. Fátima Barroso; Maria João Ramalhosa; Rita C. Alves; Alexandra Dias; Cristina M.D. Soares; Maria Teresa Oliva-Teles; Cristina Delerue-Matos. 2016. "Total antioxidant capacity of plant infusions: Assessment using electrochemical DNA-based biosensor and spectrophotometric methods." Food Control 68, no. : 153-161.
Bioremediation is an attractive and useful method of remediation of soils contaminated with petroleum hydrocarbons because it is simple to maintain, applicable in large areas, is economic and enables an effective destruction of the contaminant. Usually, the autochthone microorganisms have no ability to degrade these compounds, and otherwise, the contaminated sites have inappropriate environmental conditions for microorganism’s development. These problems can be overcome by assisted bioremediation (bioaugmentation and/or biostimulation). In this study the assisted bioremediation capacity on the rehabilitation of three natural sub-soils (granite, limestone and schist) contaminated with benzene was evaluated. Two different types of assisted bioremediation were used: without and with ventilation (bioventing). The bioaugmentation was held by inoculating the soil with a consortium of microorganisms collected from the protection area of crude oil storage tanks in a refinery. In unventilated trials, biostimulation was accomplished by the addition of a nutrient mineral media, while in bioventing oxygen was also added. The tests were carried out at controlled temperature of 25 ºC in stainless steel columns where the moist soil contaminated with benzene (200 mg per kg of soil) occupied about 40% of the column’s volume. The processes were daily monitored in discontinued mode. Benzene concentration in the gas phase was quantified by gas chromatography (GC-FID), oxygen and carbon dioxide concentrations were monitored by respirometry. The results revealed that the three contaminated soils were remediated using both technologies, nevertheless, the bioventing showed faster rates. With this work it was proved that respirometric analysis is an appropriate instrument for monitoring the biological activity.
Maria Manuela Carvalho; Maria Cristina Vila; Cristina Delerue Matos; Maria Teresa Oliva Teles; António Fiúza. Assisted bioremediation tests on three natural soils contaminated with benzene. EURASIAN JOURNAL OF SOIL SCIENCE (EJSS) 2015, 4, 153 .
AMA StyleMaria Manuela Carvalho, Maria Cristina Vila, Cristina Delerue Matos, Maria Teresa Oliva Teles, António Fiúza. Assisted bioremediation tests on three natural soils contaminated with benzene. EURASIAN JOURNAL OF SOIL SCIENCE (EJSS). 2015; 4 (3):153.
Chicago/Turabian StyleMaria Manuela Carvalho; Maria Cristina Vila; Cristina Delerue Matos; Maria Teresa Oliva Teles; António Fiúza. 2015. "Assisted bioremediation tests on three natural soils contaminated with benzene." EURASIAN JOURNAL OF SOIL SCIENCE (EJSS) 4, no. 3: 153.
We propose an immunomagnetic assay coupled to potentiometric detection suitable for sensitive (limit-of-detection 20 cells per mL) and rapid (assay time of 75 minutes) detection ofSalmonella typhimurium.
Nádia F. D. Silva; Júlia M. C. S. Magalhães; Maria Teresa Oliva-Teles; Cristina Delerue-Matos. A potentiometric magnetic immunoassay for rapid detection of Salmonella typhimurium. Analytical Methods 2015, 7, 4008 -4011.
AMA StyleNádia F. D. Silva, Júlia M. C. S. Magalhães, Maria Teresa Oliva-Teles, Cristina Delerue-Matos. A potentiometric magnetic immunoassay for rapid detection of Salmonella typhimurium. Analytical Methods. 2015; 7 (9):4008-4011.
Chicago/Turabian StyleNádia F. D. Silva; Júlia M. C. S. Magalhães; Maria Teresa Oliva-Teles; Cristina Delerue-Matos. 2015. "A potentiometric magnetic immunoassay for rapid detection of Salmonella typhimurium." Analytical Methods 7, no. 9: 4008-4011.
Maria Manuela Carvalho; Maria Cristina Vila; Fernanda Rohden; Mónica Rosas; Joana Dias; Maria Teresa Oliva-Teles; Anthony Danko; Cristina Delerue-Matos; António Fiúza. How the sorption of benzene in soils contaminated with aromatic hydrocarbons is affected by the presence of biofuels. EURASIAN JOURNAL OF SOIL SCIENCE (EJSS) 2015, 4, 111 .
AMA StyleMaria Manuela Carvalho, Maria Cristina Vila, Fernanda Rohden, Mónica Rosas, Joana Dias, Maria Teresa Oliva-Teles, Anthony Danko, Cristina Delerue-Matos, António Fiúza. How the sorption of benzene in soils contaminated with aromatic hydrocarbons is affected by the presence of biofuels. EURASIAN JOURNAL OF SOIL SCIENCE (EJSS). 2015; 4 (2):111.
Chicago/Turabian StyleMaria Manuela Carvalho; Maria Cristina Vila; Fernanda Rohden; Mónica Rosas; Joana Dias; Maria Teresa Oliva-Teles; Anthony Danko; Cristina Delerue-Matos; António Fiúza. 2015. "How the sorption of benzene in soils contaminated with aromatic hydrocarbons is affected by the presence of biofuels." EURASIAN JOURNAL OF SOIL SCIENCE (EJSS) 4, no. 2: 111.
Cadmium is a priority hazardous substance, persistent in the aquatic environment, with the capacity to interfere with crustacean moulting. Moulting is a vital process dictating crustacean growth, reproduction and metamorphosis. However, for many organisms, moult disruption is difficult to evaluate in the short term, what limits its inclusion in monitoring programmes. N-acetyl-β-d-glucosaminidase (NAGase) is an enzyme acting in the final steps of the endocrine-regulated moulting cascade, allowing for the cast off of the old exoskeleton, with potential interest as a biomarker of moult disruption. This study investigated responses to waterborne cadmium of NAGase activity of Carcinus maenas originating from estuaries with different histories of anthropogenic contamination: a low impacted and a moderately polluted one. Crabs from both sites were individually exposed for seven days to cadmium concentrations ranging from 1.3 to 2000 μg/L. At the end of the assays, NAGase activity was assessed in the epidermis and digestive gland. Detoxification, antioxidant, energy production, and oxidative stress biomarkers implicated in cadmium metabolism and tolerance were also assessed to better understand differential NAGase responses: activity of glutathione S-transferases (GST), glutathione peroxidase (GPx) glutathione reductase (GR), levels of total glutathiones (TG), lipid peroxidation (LPO), lactate dehydrogenase (LDH), and NADP+-dependent isocitrate dehydrogenase (IDH). Animals from the moderately polluted estuary had lower NAGase activity both in the epidermis and digestive gland than in the low impacted site. NAGase activity in the epidermis and digestive gland of C. maenas from both estuaries was sensitive to cadmium exposure suggesting its usefulness for inclusion in monitoring programmes. However, in the digestive gland NAGase inhibition was found in crabs from the less impacted site but not in those from the moderately contaminated one. Altered glutathione levels were observed in cadmium-treated crabs from the contaminated site possibly conferring enhanced tolerance to these animals through its chelator action. Investigation of enhanced tolerance should thus be accounted for in monitoring programmes employing NAGase as biomarker to avoid data misinterpretation
Sofia Raquel Mesquita; Şeyda Fikirdeşici Ergen; Aurélie Rodrigues; Maria Teresa Oliva-Teles; Cristina Delerue-Matos; Laura Guimarães. N-acetyl-β-d-glucosaminidase activity in feral Carcinus maenas exposed to cadmium. Aquatic Toxicology 2015, 159, 225 -232.
AMA StyleSofia Raquel Mesquita, Şeyda Fikirdeşici Ergen, Aurélie Rodrigues, Maria Teresa Oliva-Teles, Cristina Delerue-Matos, Laura Guimarães. N-acetyl-β-d-glucosaminidase activity in feral Carcinus maenas exposed to cadmium. Aquatic Toxicology. 2015; 159 ():225-232.
Chicago/Turabian StyleSofia Raquel Mesquita; Şeyda Fikirdeşici Ergen; Aurélie Rodrigues; Maria Teresa Oliva-Teles; Cristina Delerue-Matos; Laura Guimarães. 2015. "N-acetyl-β-d-glucosaminidase activity in feral Carcinus maenas exposed to cadmium." Aquatic Toxicology 159, no. : 225-232.
Concurrent exposure of estuarine organisms to man-made and natural stressors has become a common occurrence. Numerous interactions of multiple stressors causing synergistic or antagonistic effects have been described. However, limited information is available on combined effects of emerging pharmaceuticals and natural stressors. This study investigated the joint effects of the antidepressant sertraline and salinity on Carcinus maenas. To improve knowledge about interactive effects and potential vulnerability,experiments were performed with organisms from two estuaries with differing histories of exposure to environmental contamination. Biomarkers related to mode of action of sertraline were employed to assess effects of environmentally realistic concentrations of sertraline at two salinity levels. Synergism and antagonism were identified for biomarkers of cholinergic neurotransmission, energy production,anti-oxidant defences and oxidative damage. Different interactions were found for the two study sites highlighting the need to account for differences in tolerance of local ecological receptors in risk evaluations
Aurélie P. Rodrigues; Lúcia H.M.L.M. Santos; Maria Teresa Oliva-Teles; Cristina Delerue-Matos; Laura Guimarães. Joint effects of salinity and the antidepressant sertraline on the estuarine decapod Carcinus maenas. Aquatic Toxicology 2014, 156, 169 -178.
AMA StyleAurélie P. Rodrigues, Lúcia H.M.L.M. Santos, Maria Teresa Oliva-Teles, Cristina Delerue-Matos, Laura Guimarães. Joint effects of salinity and the antidepressant sertraline on the estuarine decapod Carcinus maenas. Aquatic Toxicology. 2014; 156 ():169-178.
Chicago/Turabian StyleAurélie P. Rodrigues; Lúcia H.M.L.M. Santos; Maria Teresa Oliva-Teles; Cristina Delerue-Matos; Laura Guimarães. 2014. "Joint effects of salinity and the antidepressant sertraline on the estuarine decapod Carcinus maenas." Aquatic Toxicology 156, no. : 169-178.
Fresh-cut vegetables are a successful convenient healthy food. Nowadays, the presence of new varieties of minimally processed vegetables in the market is common in response to the consumers demand for new flavours and high quality products. Within the most recent fresh-cut products are the aromatic herbs. In this work, the objective was to evaluate the nutritional quality and stability of four fresh-cut aromatic herbs. Several physicochemical quality characteristics (colour, pH, total soluble solids, and total titratable acidity) were monitored in fresh-cut chives, coriander, spearmint and parsley leaves, stored under refrigeration (3 ± 1 ºC) during 10 days. Their nutritional composition was determined, including mineral composition (phosphorous, potassium, sodium, calcium, magnesium, iron, zinc, manganese and copper) and fat- and water-soluble vitamin contents. Total soluble phenolics, flavonoids and the antioxidant capacity were determined by spectrophotometric methods. The aromatic herbs kept their fresh appearance during the storage, maintaining their colour throughout shelf-life. Their macronutrient composition and mineral content were stable during storage. Coriander had the highest mineral and fatsoluble vitamin content, while spearmint showed the best scores in the phenolic, flavonoid and antioxidant capacity assays. Vitamins and antioxidant capacity showed some variation during storage, with a differential behaviour of each compound according to the sample
J. Santos; M. Herrero; J.A. Mendiola; M.T. Oliva-Teles; E. Ibáñez; C. Delerue-Matos; M.B.P.P. Oliveira. Fresh-cut aromatic herbs: Nutritional quality stability during shelf-life. LWT - Food Science and Technology 2014, 59, 101 -107.
AMA StyleJ. Santos, M. Herrero, J.A. Mendiola, M.T. Oliva-Teles, E. Ibáñez, C. Delerue-Matos, M.B.P.P. Oliveira. Fresh-cut aromatic herbs: Nutritional quality stability during shelf-life. LWT - Food Science and Technology. 2014; 59 (1):101-107.
Chicago/Turabian StyleJ. Santos; M. Herrero; J.A. Mendiola; M.T. Oliva-Teles; E. Ibáñez; C. Delerue-Matos; M.B.P.P. Oliveira. 2014. "Fresh-cut aromatic herbs: Nutritional quality stability during shelf-life." LWT - Food Science and Technology 59, no. 1: 101-107.
An integrated chemical-biological effects monitoring was performed in 2010 and 2012 in two NW Iberian estuaries under different anthropogenic pressure. One is low impacted and the other is contaminated by metals. The aim was to verify the usefulness of a multibiomarker approach, using Carcinus maenas as bioindicator species, to reflect diminishing environmental contamination and improved health status under abiotic variation. Sampling sites were assessed for metal levels in sediments and C. maenas, water abiotic factors and biomarkers (neurotoxicity, energy metabolism, biotransformation, anti-oxidant defences, oxidative damage). High inter-annual and seasonal abiotic variation was observed. Metal levels in sediments and crab tissues were markedly higher in 2010 than in 2012 in the contaminated estuary. Biomarkers indicated differences between the study sites and seasons and an improvement of effects measured in C. maenas from the polluted estuary in 2012. Integrated Biomarker Response (IBR) index depicted sites with higher stress levels whereas Principal Component Analysis (PCA) showed associations between biomarker responses and environmental variables. The multibiomarker approach and integrated assessments proved to be useful to the early diagnosis of remediation measures in impacted sites
Aurélie Pinto Rodrigues; Teresa Oliva–Teles; Sofia Raquel Mesquita; Cristina Delerue–Matos; Laura Guimaraes. Integrated biomarker responses of an estuarine invertebrate to high abiotic stress and decreased metal contamination. Marine Environmental Research 2014, 101, 101 -114.
AMA StyleAurélie Pinto Rodrigues, Teresa Oliva–Teles, Sofia Raquel Mesquita, Cristina Delerue–Matos, Laura Guimaraes. Integrated biomarker responses of an estuarine invertebrate to high abiotic stress and decreased metal contamination. Marine Environmental Research. 2014; 101 ():101-114.
Chicago/Turabian StyleAurélie Pinto Rodrigues; Teresa Oliva–Teles; Sofia Raquel Mesquita; Cristina Delerue–Matos; Laura Guimaraes. 2014. "Integrated biomarker responses of an estuarine invertebrate to high abiotic stress and decreased metal contamination." Marine Environmental Research 101, no. : 101-114.
The mineral content (phosphorous (P), potassium (K), sodium (Na), calcium (Ca), magnesium (Mg), iron (Fe), manganese (Mn), zinc (Zn) and copper (Cu)) of eight ready-to-eat baby leaf vegetables was determined. The samples were subjected to microwave-assisted digestion and the minerals were quantified by High-Resolution Continuum Source Atomic Absorption Spectrometry (HR-CS-AAS) with flame and electrothermal atomisation. The methods were optimised and validated producing low LOQs, good repeatability and linearity, and recoveries, ranging from 91% to 110% for the minerals analysed. Phosphorous was determined by a standard colorimetric method. The accuracy of the method was checked by analysing a certified reference material; results were in agreement with the quantified value. The samples had a high content of potassium and calcium, but the principal mineral was iron. The mineral content was stable during storage and baby leaf vegetables could represent a good source of minerals in a balanced diet. A linear discriminant analysis was performed to compare the mineral profile obtained and showed, as expected, that the mineral content was similar between samples from the same family. The Linear Discriminant Analysis was able to discriminate different samples based on their mineral profile.
Joana Santos; Maria Teresa Oliva-Teles; Cristina Delerue-Matos; M.B.P.P. Oliveira. Multi-elemental analysis of ready-to-eat “baby leaf” vegetables using microwave digestion and high-resolution continuum source atomic absorption spectrometry. Food Chemistry 2014, 151, 311 -316.
AMA StyleJoana Santos, Maria Teresa Oliva-Teles, Cristina Delerue-Matos, M.B.P.P. Oliveira. Multi-elemental analysis of ready-to-eat “baby leaf” vegetables using microwave digestion and high-resolution continuum source atomic absorption spectrometry. Food Chemistry. 2014; 151 ():311-316.
Chicago/Turabian StyleJoana Santos; Maria Teresa Oliva-Teles; Cristina Delerue-Matos; M.B.P.P. Oliveira. 2014. "Multi-elemental analysis of ready-to-eat “baby leaf” vegetables using microwave digestion and high-resolution continuum source atomic absorption spectrometry." Food Chemistry 151, no. : 311-316.
Pea-shoots are a new option as ready-to-eat baby-leaf vegetable. However, data about the nutritional composition and the shelf-life stability of these leaves, especially their phytonutrient composition is scarce. In this work, the macronutrient, micronutrient and phytonutrients profile of minimally processed pea shoots were evaluated at the beginning and at the end of a 10-day storage period. Several physicochemical characteristics (color, pH, total soluble solids, and total titratable acidity) were also monitored. Standard AOAC methods were applied in the nutritional value evaluation, while chromatographic methods with UV–vis and mass detection were used to analyze free forms of vitamins (HPLC-DAD-ESI-MS/MS), carotenoids (HPLC-DAD-APCI-MSn) and flavonoid compounds (HPLC-DAD-ESI-MSn). Atomic absorption spectrometry (HR-CS-AAS) was employed to characterize the mineral content of the leaves. As expected, pea leaves had a high water (91.5%) and low fat (0.3%) and carbohydrate (1.9%) contents, being a good source of dietary fiber (2.1%). Pea shoots showed a high content of vitamins C, E and A, potassium and phosphorous compared to other ready-to-eat green leafy vegetables. The carotenoid profile revealed a high content of β-carotene and lutein, typical from green leafy vegetables. The leaves had a mean flavonoid content of 329 mg/100 g of fresh product, mainly composed by glycosylated quercetin and kaempferol derivatives. Pea shoots kept their fresh appearance during the storage being color maintained throughout the shelf-life. The nutritional composition was in general stable during storage, showing some significant (p < 0.05) variation in certain water-soluble vitamins
Joana Santos; Miguel Herrero; Jose A. Mendiola; Maria Teresa Oliva-Teles; E. Ibáñez; Cristina Delerue-Matos; Beatriz Oliveira. Assessment of nutritional and metabolic profiles of pea shoots: The new ready-to-eat baby-leaf vegetable. Food Research International 2014, 58, 105 -111.
AMA StyleJoana Santos, Miguel Herrero, Jose A. Mendiola, Maria Teresa Oliva-Teles, E. Ibáñez, Cristina Delerue-Matos, Beatriz Oliveira. Assessment of nutritional and metabolic profiles of pea shoots: The new ready-to-eat baby-leaf vegetable. Food Research International. 2014; 58 ():105-111.
Chicago/Turabian StyleJoana Santos; Miguel Herrero; Jose A. Mendiola; Maria Teresa Oliva-Teles; E. Ibáñez; Cristina Delerue-Matos; Beatriz Oliveira. 2014. "Assessment of nutritional and metabolic profiles of pea shoots: The new ready-to-eat baby-leaf vegetable." Food Research International 58, no. : 105-111.
Among aminoacidopathies, phenylketonuria (PKU) is the most prevalent one.
Filipa B. Pimentel; Rita C. Alves; Maria Teresa Oliva-Teles; Anabela S. G. Costa; Telmo J. R. Fernandes; Manuela F. Almeida; Duarte Torres; Cristina Delerue-Matos; M. Beatriz. P. P. Oliveira. Targeting specific nutrient deficiencies in protein-restricted diets: some practical facts in PKU dietary management. Food & Function 2014, 5, 3151 -3159.
AMA StyleFilipa B. Pimentel, Rita C. Alves, Maria Teresa Oliva-Teles, Anabela S. G. Costa, Telmo J. R. Fernandes, Manuela F. Almeida, Duarte Torres, Cristina Delerue-Matos, M. Beatriz. P. P. Oliveira. Targeting specific nutrient deficiencies in protein-restricted diets: some practical facts in PKU dietary management. Food & Function. 2014; 5 (12):3151-3159.
Chicago/Turabian StyleFilipa B. Pimentel; Rita C. Alves; Maria Teresa Oliva-Teles; Anabela S. G. Costa; Telmo J. R. Fernandes; Manuela F. Almeida; Duarte Torres; Cristina Delerue-Matos; M. Beatriz. P. P. Oliveira. 2014. "Targeting specific nutrient deficiencies in protein-restricted diets: some practical facts in PKU dietary management." Food & Function 5, no. 12: 3151-3159.