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The United Nations (UN) 2030 agenda on sustainable development goals (SDGs) encourages us to implement sustainable infrastructure and services for confronting challenges such as large energy consumption, solid waste generation, depletion of water resources and emission of greenhouse gases in the construction industry. Therefore, to overcome challenges and establishing sustainable construction, there is a requirement to integrate information technology with innovative manufacturing processes and materials science. Moreover, the wide implementation of three-dimensional printing (3DP) technology in constructing monuments, artistic objects, and residential buildings has gained attention. The integration of the Internet of Things (IoT), cloud manufacturing (CM), and 3DP allows us to digitalize the construction for providing reliable and digitalized features to the users. In this review article, we discuss the opportunities and challenges of implementing the IoT, CM, and 3D printing (3DP) technologies in building constructions for achieving sustainability. The recent convergence research of cloud development and 3D printing (3DP) are being explored in the article by categorizing them into multiple sections including 3D printing resource access technology, 3D printing cloud platform (3D–PCP) service architectures, 3D printing service optimized configuration technology, 3D printing service evaluation technology, and 3D service control and monitoring technology. This paper also examines and analyzes the limitations of existing research and, moreover, the article provides key recommendations such as automation with robotics, predictive analytics in 3DP, eco-friendly 3DP, and 5G technology-based IoT-based CM for future enhancements.
Rajesh Singh; Anita Gehlot; Shaik Akram; Lovi Gupta; Manoj Jena; Chander Prakash; Sunpreet Singh; Raman Kumar. Cloud Manufacturing, Internet of Things-Assisted Manufacturing and 3D Printing Technology: Reliable Tools for Sustainable Construction. Sustainability 2021, 13, 7327 .
AMA StyleRajesh Singh, Anita Gehlot, Shaik Akram, Lovi Gupta, Manoj Jena, Chander Prakash, Sunpreet Singh, Raman Kumar. Cloud Manufacturing, Internet of Things-Assisted Manufacturing and 3D Printing Technology: Reliable Tools for Sustainable Construction. Sustainability. 2021; 13 (13):7327.
Chicago/Turabian StyleRajesh Singh; Anita Gehlot; Shaik Akram; Lovi Gupta; Manoj Jena; Chander Prakash; Sunpreet Singh; Raman Kumar. 2021. "Cloud Manufacturing, Internet of Things-Assisted Manufacturing and 3D Printing Technology: Reliable Tools for Sustainable Construction." Sustainability 13, no. 13: 7327.
Colorectal cancer (CRC) is the World’s third most frequently diagnosed cancer type. It accounted for about 9.4% mortality out of the total incidences of cancer in the year 2020. According to estimated facts by World Health Organization (WHO), by 2030, 27 million new CRC cases, 17 million deaths, and around 75 million people living with the disease will appear. The facts and evidence that establish a link between the intestinal microflora and the occurrence of CRC are quite intuitive. Current shortcomings of chemo- and radiotherapies and the unavailability of appropriate treatment strategies for CRC are becoming the driving force to search for an alternative approach for the prevention, therapy, and management of CRC. Probiotics have been used for a long time due to their beneficial health effects, and now, it has become a popular candidate for the preventive and therapeutic treatment of CRC. The probiotics adopt different strategies such as the improvement of the intestinal barrier function, balancing of natural gut microflora, secretion of anticancer compounds, and degradation of carcinogenic compounds, which are useful in the prophylactic treatment of CRC. The pro-apoptotic ability of probiotics against cancerous cells makes them a potential therapeutic candidate against cancer diseases. Moreover, the immunomodulatory properties of probiotics have created interest among researchers to explore the therapeutic strategy by activating the immune system against cancerous cells. The present review discusses in detail different strategies and mechanisms of probiotics towards the prevention and treatment of CRC.
Ashutosh Tripathy; Jayalaxmi Dash; Sudhakar Kancharla; Prachetha Kolli; Deviyani Mahajan; Shantibhusan Senapati; Manoj Jena. Probiotics: A Promising Candidate for Management of Colorectal Cancer. Cancers 2021, 13, 3178 .
AMA StyleAshutosh Tripathy, Jayalaxmi Dash, Sudhakar Kancharla, Prachetha Kolli, Deviyani Mahajan, Shantibhusan Senapati, Manoj Jena. Probiotics: A Promising Candidate for Management of Colorectal Cancer. Cancers. 2021; 13 (13):3178.
Chicago/Turabian StyleAshutosh Tripathy; Jayalaxmi Dash; Sudhakar Kancharla; Prachetha Kolli; Deviyani Mahajan; Shantibhusan Senapati; Manoj Jena. 2021. "Probiotics: A Promising Candidate for Management of Colorectal Cancer." Cancers 13, no. 13: 3178.
The extracellular matrix (ECM) plays an important role in the evolution of early metazoans, as it provides structural and biochemical support to the surrounding cells through the cell–cell and cell–matrix interactions. In multi-cellular organisms, ECM plays a pivotal role in the differentiation of tissues and in the development of organs. Fibulins are ECM glycoproteins, found in a variety of tissues associated with basement membranes, elastic fibers, proteoglycan aggregates, and fibronectin microfibrils. The expression profile of fibulins reveals their role in various developmental processes such as elastogenesis, development of organs during the embryonic stage, tissue remodeling, maintenance of the structural integrity of basement membrane, and elastic fibers, as well as other cellular processes. Apart from this, fibulins are also involved in the progression of human diseases such as cancer, cardiac diseases, congenital disorders, and chronic fibrotic disorders. Different isoforms of fibulins show a dual role of tumor-suppressive and tumor-promoting activities, depending on the cell type and cellular microenvironment in the body. Knockout animal models have provided deep insight into their role in development and diseases. The present review covers details of the structural and expression patterns, along with the role of fibulins in embryonic development and disease progression, with more emphasis on their involvement in the modulation of cancer diseases.
Deviyani Mahajan; Sudhakar Kancharla; Prachetha Kolli; Amarish Sharma; Sanjeev Singh; Sudarshan Kumar; Ashok Mohanty; Manoj Jena. Role of Fibulins in Embryonic Stage Development and Their Involvement in Various Diseases. Biomolecules 2021, 11, 685 .
AMA StyleDeviyani Mahajan, Sudhakar Kancharla, Prachetha Kolli, Amarish Sharma, Sanjeev Singh, Sudarshan Kumar, Ashok Mohanty, Manoj Jena. Role of Fibulins in Embryonic Stage Development and Their Involvement in Various Diseases. Biomolecules. 2021; 11 (5):685.
Chicago/Turabian StyleDeviyani Mahajan; Sudhakar Kancharla; Prachetha Kolli; Amarish Sharma; Sanjeev Singh; Sudarshan Kumar; Ashok Mohanty; Manoj Jena. 2021. "Role of Fibulins in Embryonic Stage Development and Their Involvement in Various Diseases." Biomolecules 11, no. 5: 685.
Proliferation and differentiation of mammary epithelial cells (MECs) is mediated by a complex cross-talk between cell and matrix, interaction among cells, hormones and growth factors. Differentiation of MECs leads to the synthesis and secretion of milk. The mechanism of switching from proliferation to differentiation is yet to be discovered at the molecular level. Differentiation is indicated by the formation of dome in in vitro cultured MECs, the first-ever change phenotypically. The present study was performed in vitro to identify the proteins expressed during initiation of differentiation of MECs in the early stage of lactogenesis using the buffalo MEC (BuMEC) cell line established in our laboratory. The cell line used was in 25th–30th passage. Differentiation was induced using lactogenic hormones (insulin, prolactin, and cortisol) and subcellular fractions (cytosolic and mitochondrial) from proliferative as well as differentiation stage were used to capture differentially expressed proteins (DEPs) by 2D DIGE and MALDI-TOF mass spectrometry. The signaling pathways associated with the DEPs were observed through bioinformatics analysis. The proteomics data were validated by qPCR analysis of selected proteins. The annexins such as annexin-I, II, and V and the S100 proteins (S100A4, S100A2, and S100A11) may have a crucial role in the initiation of differentiation and subsequently lactogenesis by MECs.
Manoj K. Jena; Vijay Anand; Shalini Jaswal; Sudarshan Kumar; Thota. J. Reddy; Ashok K. Mohanty. DIGE-based identification of preferentially expressed proteins in early stage of lactogenic differentiation in buffalo (Bubalus bubalis) mammary epithelial cells. Journal of Proteins and Proteomics 2021, 12, 19 -31.
AMA StyleManoj K. Jena, Vijay Anand, Shalini Jaswal, Sudarshan Kumar, Thota. J. Reddy, Ashok K. Mohanty. DIGE-based identification of preferentially expressed proteins in early stage of lactogenic differentiation in buffalo (Bubalus bubalis) mammary epithelial cells. Journal of Proteins and Proteomics. 2021; 12 (1):19-31.
Chicago/Turabian StyleManoj K. Jena; Vijay Anand; Shalini Jaswal; Sudarshan Kumar; Thota. J. Reddy; Ashok K. Mohanty. 2021. "DIGE-based identification of preferentially expressed proteins in early stage of lactogenic differentiation in buffalo (Bubalus bubalis) mammary epithelial cells." Journal of Proteins and Proteomics 12, no. 1: 19-31.
The recent outbreak of the novel coronavirus (SARS-CoV-2) in the Wuhan province of China has taken millions of lives worldwide. In this pandemic situation and absence of known drugs and vaccines against novel coronavirus disease (COVID-19), there is an urgent need for the repurposing of the existing drugs against it. So, here we have examined a safe and cheap alternative against this virus by screening hundreds of nutraceuticals compounds against known therapeutic targets of SARS-COV-2 by molecular docking. The virtual screening results were then analyzed for binding energy and interactive residues and compared with some already known hits in the best binding pose. All these analyses of this study strongly predicted the potential of Folic acid and its derivates like Tetrahydrofolic acid and 5-methyl tetrahydrofolic acid against SARS-COV-2. The strong and stable binding affinity of this water-soluble vitamin and its derivatives against the SARS-COV-2, indicating that they could be valuable drugs against the management of this COVID-19 pandemic. This study could serve as the starting point for further investigation of these molecules through in vitro and in vivo assays.
Vipul Kumar; Sudhakar Kancharla; Manoj Kumar Jena. In silico virtual screening-based study of nutraceuticals predicts the therapeutic potentials of folic acid and its derivatives against COVID-19. VirusDisease 2021, 32, 29 -37.
AMA StyleVipul Kumar, Sudhakar Kancharla, Manoj Kumar Jena. In silico virtual screening-based study of nutraceuticals predicts the therapeutic potentials of folic acid and its derivatives against COVID-19. VirusDisease. 2021; 32 (1):29-37.
Chicago/Turabian StyleVipul Kumar; Sudhakar Kancharla; Manoj Kumar Jena. 2021. "In silico virtual screening-based study of nutraceuticals predicts the therapeutic potentials of folic acid and its derivatives against COVID-19." VirusDisease 32, no. 1: 29-37.
Background: The novel severe acute respiratory syndrome related corona virus-2 (SARS-CoV-2) belongs to the “Coronaviridae” family and order “Nidovirales”, which has caused the pandemic coronavirus disease 2019 (COVID-19). SARS-CoV-2 has been spread in more than a 100 countries, and more than a million have lost their lives. Vaccination and immunization could be an effective strategy to combat fatal COVID-19. Methods: For identification of effective vaccine candidate against COVID-19, various immunoinformatics online tools and softwares were used to predict epitopes. Cytotoxic T cell epitopes, helper T cell epitopes, and B cell epitopes from three structural polyproteins (Spike, Membrane, and Nucleocapsid (SMN) based on the binding affinity towards MHC, antigenicity, non-allergenicity, and non-toxicity) were identified for vaccine development. The multiepitope based vaccine was constructed linking two additional adjuvants human beta-defensin-3 and human beta-defensin-2 at N and C terminal, respectively. Results: The constructed vaccine sequence was found to be a good antigen and non-allergen for the human body. The constructed vaccine was docked with the TLR-3 receptor. The docked complex was further taken for molecular dynamics simulations and RMSD was calculated, which showed stable binding of the complex. The codon adaptation index (CAI) of 0.92 and GC content of 55.5% for E. coli (K12 strain) suggested efficient expression of the predicted vaccine. Conclusion: The current study can be helpful in the reduction of time and cost for further experimental validations and could give a valuable contribution against this pandemic.
Vipul Kumar; Sudhakar Kancharla; Prachetha Kolli; Manoj Jena. Reverse vaccinology approach towards the in-silico multiepitope vaccine development against SARS-CoV-2. F1000Research 2021, 10, 44 .
AMA StyleVipul Kumar, Sudhakar Kancharla, Prachetha Kolli, Manoj Jena. Reverse vaccinology approach towards the in-silico multiepitope vaccine development against SARS-CoV-2. F1000Research. 2021; 10 ():44.
Chicago/Turabian StyleVipul Kumar; Sudhakar Kancharla; Prachetha Kolli; Manoj Jena. 2021. "Reverse vaccinology approach towards the in-silico multiepitope vaccine development against SARS-CoV-2." F1000Research 10, no. : 44.
Preeclampsia (PE) is a serious pregnancy complication, affecting about 5–7% of pregnancies worldwide and is characterized by hypertension and damage to multiple maternal organs, primarily the liver and kidneys. PE usually begins after 20 weeks’ gestation and, if left untreated, can lead to serious complications and lifelong disabilities—even death—in both the mother and the infant. As delivery is the only cure for the disease, treatment is primarily focused on the management of blood pressure and other clinical symptoms. The pathogenesis of PE is still not clear. Abnormal spiral artery remodeling, placental ischemia and a resulting increase in the circulating levels of vascular endothelial growth factor receptor-1 (VEGFR-1), also called soluble fms-like tyrosine kinase-1 (sFlt-1), are believed to be among the primary pathologies associated with PE. sFlt-1 is produced mainly in the placenta during pregnancy and acts as a decoy receptor, binding to free VEGF (VEGF-A) and placental growth factor (PlGF), resulting in the decreased bioavailability of each to target cells. Despite the pathogenic effects of increased sFlt-1 on the maternal vasculature, recent studies from our laboratory and others have strongly indicated that the increase in sFlt-1 in PE may fulfill critical protective functions in preeclamptic pregnancies. Thus, further studies on the roles of sFlt-1 in normal and preeclamptic pregnancies are warranted for the development of therapeutic strategies targeting VEGF signaling for the treatment of PE. Another impediment to the treatment of PE is the lack of suitable methods for delivery of cargo to placental cells, as PE is believed to be of placental origin and most available therapies for PE adversely impact both the mother and the fetus. The present review discusses the pathogenesis of PE, the complex role of sFlt-1 in maternal disease and fetal protection, and the recently developed placenta-targeted drug delivery system for the potential treatment of PE with candidate therapeutic agents.
Manoj Kumar Jena; Neeta Raj Sharma; Matthew Petitt; Devika Maulik; Nihar Ranjan Nayak. Pathogenesis of Preeclampsia and Therapeutic Approaches Targeting the Placenta. Biomolecules 2020, 10, 953 .
AMA StyleManoj Kumar Jena, Neeta Raj Sharma, Matthew Petitt, Devika Maulik, Nihar Ranjan Nayak. Pathogenesis of Preeclampsia and Therapeutic Approaches Targeting the Placenta. Biomolecules. 2020; 10 (6):953.
Chicago/Turabian StyleManoj Kumar Jena; Neeta Raj Sharma; Matthew Petitt; Devika Maulik; Nihar Ranjan Nayak. 2020. "Pathogenesis of Preeclampsia and Therapeutic Approaches Targeting the Placenta." Biomolecules 10, no. 6: 953.
The novel severe acute respiratory syndrome related corona virus-2 (SARS-CoV-2) belongs to the “Coronaviridae” family and order “Nidovirales” cause coronavirus disease (COVID19). The SARS-CoV-2 has been spread in more than a hundred countries, and more than a million have lost their lives. Recently COVID-19 has been declared as pandemic worldwide. Vaccination and immunization could only be an effective strategy to combat this fatal COVID-19. For identification of the effective vaccine candidate against COVID-19, various immunoinformatics online tools and software were used to predict the epitopes. The cytotoxic T cell epitopes, Helper T cell epitopes, and B cell epitopes from three structural polyproteins ( Spike, Membrane, and Nucleocapsid (SMN) ) based on the binding affinity towards MHC , antigenicity, non-allergenicity, and non-toxicity were identified for vaccine development. The multiepitope based vaccine was constructed linking two additional adjuvants human betadefensin-3 and human beta-defensin-2 at N and C terminal, respectively. Constructed vaccine sequence was found to be a good antigen and non-allergen for the human body. The constructed vaccine was docked with the TLR-3 receptor. And the docked complex then further taken for Molecular dynamics simulations and RMSD was calculated, which showed stable binding of the complex. The codon adaptation index (CAI) of 0.92 and GC content of 55.5% for E.coli (k12) strain suggested the efficient expression of the predicted vaccine. The current study can be helpful in the reduction of time and cost for further experimental validations and could give a valuable contribution against this pandemic.
Vipul Kumar; Manoj Jena. Reverse vaccinology approach towards the in-silico multiepitope vaccine development against SARS-CoV-2. 2020, 1 .
AMA StyleVipul Kumar, Manoj Jena. Reverse vaccinology approach towards the in-silico multiepitope vaccine development against SARS-CoV-2. . 2020; ():1.
Chicago/Turabian StyleVipul Kumar; Manoj Jena. 2020. "Reverse vaccinology approach towards the in-silico multiepitope vaccine development against SARS-CoV-2." , no. : 1.
The recent outbreak of the novel coronavirus (SARS-CoV-2) in the Wuhan province of China has taken millions of lives worldwide. In this pandemic situation and absence of known drugs and vaccines against novel coronavirus disease (COVID-19), there is an urgent need for the repurposing of the existing drugs against it. So, here we have examined a safe and cheap alternative against this virus by screening hundreds of nutraceuticals compounds against known therapeutic targets of SARS-COV-2 by molecular docking .The virtual screening results were then analyzed for binding energy and interactive residues in the best binding pose. All these analyses of this study strongly predicted the potential of Folic acid and its derivates like Tetrahydrofolic acid and 5-methyl tetrahydrofolic acid against SARS-COV-2. The strong and stable binding affinity of this water-soluble vitamin and its derivatives against the SARS-COV-2, indicating that they could be valuable drugs against the management of this COVID-19 pandemic. This study could serve as the starting point for further investigation of these molecules through in-vitro and in-vivo assays.
Vipul Kumar; Manoj Jena. In silico virtual screening-based study of nutraceuticals predicts the therapeutic potentials of folic acid and its derivatives against COVID-19. 2020, 1 .
AMA StyleVipul Kumar, Manoj Jena. In silico virtual screening-based study of nutraceuticals predicts the therapeutic potentials of folic acid and its derivatives against COVID-19. . 2020; ():1.
Chicago/Turabian StyleVipul Kumar; Manoj Jena. 2020. "In silico virtual screening-based study of nutraceuticals predicts the therapeutic potentials of folic acid and its derivatives against COVID-19." , no. : 1.
Macrophages (MФs) are the leukocytes produced from differentiation of monocytes and are located in almost all tissues of human body. They are involved in various processes, such as phagocytosis, innate and adaptive immunity, proinflammatory (M1) and anti-inflammatory (M2) activity, depending on the tissue microenvironment. They play a crucial role in pregnancy, and their dysfunction or alteration of polarity is involved in pregnancy disorders, like preeclampsia, recurrent spontaneous abortion, infertility, intrauterine growth restriction, and preterm labor. About 50–60% of decidual leukocytes are natural killer (NK) cells followed by MФs (the second largest population). MФs are actively involved in trophoblast invasion, tissue and vascular remodeling during early pregnancy, besides their role as major antigen-presenting cells in the decidua. These cells have different phenotypes and polarities in different stages of pregnancy. They have also been observed to enhance tumor growth by their anti-inflammatory activity (M2 type) and prevent immunogenic rejection. Targeted alteration of polarity (M1–M2 or vice versa) could be a major focus in the future treatment of pregnancy complications. This review is focused on the role of MФs in pregnancy, their involvement in pregnancy disorders, and decidual MФs as possible therapeutic targets for the treatment of pregnancy complications.
Manoj K. Jena; Neha Nayak; Kang Chen; Nihar R. Nayak. Role of Macrophages in Pregnancy and Related Complications. Archivum Immunologiae et Therapiae Experimentalis 2019, 67, 295 -309.
AMA StyleManoj K. Jena, Neha Nayak, Kang Chen, Nihar R. Nayak. Role of Macrophages in Pregnancy and Related Complications. Archivum Immunologiae et Therapiae Experimentalis. 2019; 67 (5):295-309.
Chicago/Turabian StyleManoj K. Jena; Neha Nayak; Kang Chen; Nihar R. Nayak. 2019. "Role of Macrophages in Pregnancy and Related Complications." Archivum Immunologiae et Therapiae Experimentalis 67, no. 5: 295-309.
Objective: This study was focused on to compare the antimicrobial activity of methanolic leaf extracts of tulsi and neem.Methods: We have chosen tulsi (Ocimum sanctum) and neem (Azadirachta indica) to compare their antimicrobial activity toward Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. Phytochemical extracts have been made by the use of methanol as solvent and dried leaf powder by the cold maceration extraction process. Phytochemical analysis for some secondary metabolites has been done using standard protocols. Nutrient agar plates were inoculated with the above-mentioned microorganisms by spreading bacterial inoculum on the surface of the media. Wells (6 mm in diameter) were punched in the agar. The phytochemical extracts of neem and tulsi were allowed to diffuse into the medium, and after incubation of 24 h at 37°C, the zones of inhibition were observed.Results: Statistical analysis showed that tulsi was more effective toward S. aureus while neem was more effective toward E. coli. Minimum inhibitory concentration (MIC) of tulsi for both the microorganisms was 0.4 g/ml, and the MIC of neem for both the microorganisms was 0.2 g/ml. When both the tulsi and neem extract were mixed with each other for every concentration at equal volume, they have shown better effects in comparison to individual neem or tulsi extract and also the MIC got reduced to 0.2 g/ml for both the bacteria.Conclusions: Leaf extracts of both the A. indica and O. sanctum have shown antimicrobial activity against E. coli and S. aureus. A. indica has higher antimicrobial activity against S. aureus, whereas the O. sanctum was found to be more effective against E. coli (indicated by the zone of inhibition). When both the extracts were mixed with each other, they have shown a better effect toward both bacteria.
Vipul Kumar; Anurag Chakraborty; Manpreet Kaur; Sony Pandey; Manoj Kumar Jena. COMPARATIVE STUDY ON ANTIMICROBIAL ACTIVITY OF TULSI (OCIMUM SANCTUM) AND NEEM (AZADIRACHTA INDICA) METHANOL EXTRACT. Asian Journal of Pharmaceutical and Clinical Research 2018, 11, 514 -517.
AMA StyleVipul Kumar, Anurag Chakraborty, Manpreet Kaur, Sony Pandey, Manoj Kumar Jena. COMPARATIVE STUDY ON ANTIMICROBIAL ACTIVITY OF TULSI (OCIMUM SANCTUM) AND NEEM (AZADIRACHTA INDICA) METHANOL EXTRACT. Asian Journal of Pharmaceutical and Clinical Research. 2018; 11 (12):514-517.
Chicago/Turabian StyleVipul Kumar; Anurag Chakraborty; Manpreet Kaur; Sony Pandey; Manoj Kumar Jena. 2018. "COMPARATIVE STUDY ON ANTIMICROBIAL ACTIVITY OF TULSI (OCIMUM SANCTUM) AND NEEM (AZADIRACHTA INDICA) METHANOL EXTRACT." Asian Journal of Pharmaceutical and Clinical Research 11, no. 12: 514-517.
The mammary gland (MG) is a unique organ responsible for milk synthesis, secretion, and involution to prepare the gland for subsequent lactation. The mammary epithelial cells (MECs), which are the milk synthesizing units of the MG, proliferate, differentiate, undergo apoptosis and regenerate following a cyclic pathway of lactation – involution – lactation, fine-tuning these molecular events through hormones, growth factors and other regulatory molecules. The developmental stages of the MG are embryonic, prepubertal, pubertal, pregnancy, lactation and involution, with major developmental processes occurring after puberty. The involution stage includes interesting physiological processes such as MEC apoptosis, matrix remodeling, and the generation of cells regaining the shape of a virgin MG. Signal transducer and activator of transcription 3 (STAT3) is the established master regulator of this process and aberrant expression of STAT3 leads to subnormal involution and may induce neoplasia. Several studies have reported on the molecular mechanism of MG involution with substantial knowledge being gained about this process; however, a deep understanding of this phenomenon has yet to be attained. This review focuses deeply on the molecular details of post-lactational regression, the signaling pathways involved in the lactation-involution cycle, and the latest developments in STAT3-associated MG neoplasia. Deep insight into the involution process will pave the way towards understanding the biology, apoptosis, and oncogenesis of the MG.
Manoj Kumar Jena; Shalini Jaswal; Sudarshan Kumar; Ashok Kumar Mohanty. Molecular mechanism of mammary gland involution: An update. Developmental Biology 2018, 445, 145 -155.
AMA StyleManoj Kumar Jena, Shalini Jaswal, Sudarshan Kumar, Ashok Kumar Mohanty. Molecular mechanism of mammary gland involution: An update. Developmental Biology. 2018; 445 (2):145-155.
Chicago/Turabian StyleManoj Kumar Jena; Shalini Jaswal; Sudarshan Kumar; Ashok Kumar Mohanty. 2018. "Molecular mechanism of mammary gland involution: An update." Developmental Biology 445, no. 2: 145-155.
Evidence is increasing on the crucial role of the extracellular matrix (ECM) in breast cancer progression, invasion and metastasis with almost all mortality cases owing to metastasis. The epithelial-mesenchymal transition is the first signal of metastasis involving different transcription factors such as Snail, TWIST, and ZEB1. ECM remodeling is a major event promoting cancer invasion and metastasis; where matrix metalloproteinases (MMPs) such as MMP-2, -9, -11, and -14 play vital roles degrading the matrix proteins for cancer spread. The β-D mannuronic acid (MMP inhibitor) has anti-metastatic properties through inhibition of MMP-2, and -9 and could be a potential therapeutic agent. Besides the MMPs, the enzymes such as LOXL2, LOXL4, procollagen lysyl hydroxylase-2, and heparanase also regulate breast cancer progression. The important ECM proteins like integrins (b1-, b5-, and b6- integrins), ECM1 protein, and Hic-5 protein are also actively involved in breast cancer development. The stromal cells such as tumor-associated macrophages (TAMs), cancer-associated fibroblasts (CAFs), and adipocytes also contribute in tumor development through different processes. The TAMs become proangiogenic through secretion of VEGF-A and building vessel network for nourishment and invasion of the tumor mass. The latest developments of ECM involvement in breast cancer progression has been discussed in this review and this study will help researchers in designing future work on breast cancer pathogenesis and developing therapy targeted to the ECM components.
Manoj Kumar Jena; Jagadeesh Janjanam. Role of extracellular matrix in breast cancer development: a brief update. F1000Research 2018, 7, 274 .
AMA StyleManoj Kumar Jena, Jagadeesh Janjanam. Role of extracellular matrix in breast cancer development: a brief update. F1000Research. 2018; 7 ():274.
Chicago/Turabian StyleManoj Kumar Jena; Jagadeesh Janjanam. 2018. "Role of extracellular matrix in breast cancer development: a brief update." F1000Research 7, no. : 274.
The crosstalk between fetus and mother starts with the onset of placental attachment to the uterus. The cotyledons and caruncles are the two anatomically distinct structures that play a crucial role in this physiological communication. Using Agilent Gene Chip Genome microarray, we measured the expression profile of pregnancy cotyledons in comparison to caruncular reminiscence of the uteri in non-pregnant buffalo (Bubalus bubalis) for the detection of the early post-pregnancy rapid changes in cellular expression of mRNA transcripts. We identified a total of 497 up- and 578 down-regulated genes with <0.05 the FDR corrected p-values using 4 replicates in each group (cotyledons and caruncles) and their role in pregnancy. Deep bioinformatics analysis of data revealed the cluster of genes involved at the placentome level for various functions such as fetus attachment, transport of nutrition, and immune response. Importantly, the pathways like Hedgehog/Calcium/Wnt signalling, cell cycle regulation and immune responses regulatory functions were highly enriched by the differentially identified genes. A very highly up-regulated IL-2 specific gene showed the role of interleukin-2 signalling in the attachment of the embryo. It was observed that the genes responsible for immune response were downregulated, suggesting an immune suppressive environment which is required to adopt the semiallogeneic fetus for a successful pregnancy. To further evaluate and validate the data, we have performed qRT-PCR analysis of twenty-one genes. The present study highlights the repertoire of active transcripts in the junction of cotyledons and caruncles, which are essential for a successful onset and completion of pregnancy.
Masoud Lotfan; Syed Azmal Ali; Munna Lal Yadav; Suman Choudhary; Manoj Kumar Jena; Sudarshan Kumar; Ashok Kumar Mohanty. Genome-wide gene expression analysis of 45 days pregnant fetal cotyledons vis-a-vis non-pregnant caruncles in buffalo ( Bubalus bubalis ). Gene 2018, 654, 127 -137.
AMA StyleMasoud Lotfan, Syed Azmal Ali, Munna Lal Yadav, Suman Choudhary, Manoj Kumar Jena, Sudarshan Kumar, Ashok Kumar Mohanty. Genome-wide gene expression analysis of 45 days pregnant fetal cotyledons vis-a-vis non-pregnant caruncles in buffalo ( Bubalus bubalis ). Gene. 2018; 654 ():127-137.
Chicago/Turabian StyleMasoud Lotfan; Syed Azmal Ali; Munna Lal Yadav; Suman Choudhary; Manoj Kumar Jena; Sudarshan Kumar; Ashok Kumar Mohanty. 2018. "Genome-wide gene expression analysis of 45 days pregnant fetal cotyledons vis-a-vis non-pregnant caruncles in buffalo ( Bubalus bubalis )." Gene 654, no. : 127-137.
Evidence is increasing on the crucial role of the extracellular matrix (ECM) in breast cancer progression, invasion and metastasis with almost all mortality cases owing to metastasis. The epithelial-mesenchymal transition is the first signal of metastasis involving different transcription factors such as Snail, TWIST, and ZEB1. ECM remodeling is a major event promoting cancer invasion and metastasis; where matrix metalloproteinases (MMPs) such as MMP-2, -9, -11, and -14 play vital roles degrading the matrix proteins for cancer spread. The β-D mannuronic acid (MMP inhibitor) has anti-metastatic properties through inhibition of MMP-2, and -9 and could be a potential therapeutic agent. Besides the MMPs, the enzymes such as LOXL2, LOXL4, procollagen lysyl hydroxylase-2, and heparanase also regulate breast cancer progression. The important ECM proteins like integrins (b1-, b5-, and b6- integrins), ECM1 protein, and Hic-5 protein are also actively involved in breast cancer development. The stromal cells such as tumor-associated macrophages (TAMs), cancer-associated fibroblasts (CAFs), and adipocytes also contribute in tumor development through different processes. The TAMs become proangiogenic through secretion of VEGF-A and building vessel network for nourishment and invasion of the tumor mass. The latest developments of ECM involvement in breast cancer progression has been discussed in this review and this study will help researchers in designing future work on breast cancer pathogenesis and developing therapy targeted to the ECM components.
Manoj Kumar Jena; Jagadeesh Janjanam. Role of extracellular matrix in breast cancer development: a brief update. F1000Research 2018, 7, 274 .
AMA StyleManoj Kumar Jena, Jagadeesh Janjanam. Role of extracellular matrix in breast cancer development: a brief update. F1000Research. 2018; 7 ():274.
Chicago/Turabian StyleManoj Kumar Jena; Jagadeesh Janjanam. 2018. "Role of extracellular matrix in breast cancer development: a brief update." F1000Research 7, no. : 274.
Jaundice is a very well-known disease found worldwide. Jaundice comes from the French word “Jaune” - which means yellow. In medical term, jaundice is known as icterus which is a Greek word. This is a very common disease in the population, which causes the yellowish or greenish pigmentation in the skin and whiteness in the eyes. This is a condition of hyperbilirubinemia in which the amount of bilirubin increases in the blood. In this case, the high amount of bilirubin is found in blood, and the disruption of the movement of bilirubin into the liver and out of the body causes jaundice. Different symptoms seen in this case are yellow skin, yellow/white eyes, dark or reddish urine, loss of appetite, bitter taste of tongue, pale faces, nausea, itching in skin, anfd slow pulse rate. Jaundice may be mild to severe. Different types of jaundice are seen like normal jaundice in newborn, hepatic jaundice, and post-hepatic Jaundice.
Manoj Jena; Anshurekha Dash; Shekhar Mohapatra S. YELLOWNESS IS A THREAT TO NEWBORN - A REVIEW. Asian Journal of Pharmaceutical and Clinical Research 2018, 11, 43 -47.
AMA StyleManoj Jena, Anshurekha Dash, Shekhar Mohapatra S. YELLOWNESS IS A THREAT TO NEWBORN - A REVIEW. Asian Journal of Pharmaceutical and Clinical Research. 2018; 11 (2):43-47.
Chicago/Turabian StyleManoj Jena; Anshurekha Dash; Shekhar Mohapatra S. 2018. "YELLOWNESS IS A THREAT TO NEWBORN - A REVIEW." Asian Journal of Pharmaceutical and Clinical Research 11, no. 2: 43-47.
It is increasingly evident that cytokines and growth factors produced in the decidua play a pivotal role in the regulation of the local immune microenvironment and the establishment of pregnancy. One of the major growth factors produced in the decidua is vascular endothelial growth factor (VEGF), which acts not only on endothelial cells, but also on multiple other cell types, including macrophages. We sought to determine whether decidua-derived VEGF affects macrophage recruitment and polarization using human endometrial/decidual tissue samples, primary human endometrial stromal cells (ESCs), and the human monocyte cell line THP1. In situ hybridization was used for assessment of local VEGF expression and immunohistochemistry was used for identification and localization of CD68-positive endometrial macrophages. Macrophage migration in culture was assessed using a transwell migration assay, and the various M1/M2 phenotypic markers and VEGF expression were assessed using quantitative real-time PCR (qRT-PCR). We found dramatic increases in both VEGF levels and macrophage numbers in the decidua during early pregnancy compared to the secretory phase endometrium (non-pregnant), with a significant increase in M2 macrophage markers, suggesting that M2 is the predominant macrophage phenotype in the decidua. However, decidual samples from preeclamptic pregnancies showed a significant shift in macrophage phenotype markers, with upregulation of M1 and downregulation of M2 markers. In THP1 cultures, VEGF treatment significantly enhanced macrophage migration and induced M1 macrophages to shift to an M2 phenotype. Moreover, treatment with conditioned media from decidualized ESCs induced changes in macrophage migration and polarization similar to that of VEGF treatment. These effects were abrogated by the addition of a potent VEGF inhibitor. Together these results suggest that decidual VEGF plays a significant role in macrophage recruitment and M2 polarization, and that inhibition of VEGF signaling may contribute to the shift in macrophage polarity observed in different pregnancy disorders, including preeclampsia.
Karen C. Wheeler; Manoj Jena; Bhola Pradhan; Neha Nayak; Subhendu Das; Chaur-Dong Hsu; David S. Wheeler; Kang Chen; Nihar R. Nayak. VEGF may contribute to macrophage recruitment and M2 polarization in the decidua. PLoS ONE 2018, 13, e0191040 .
AMA StyleKaren C. Wheeler, Manoj Jena, Bhola Pradhan, Neha Nayak, Subhendu Das, Chaur-Dong Hsu, David S. Wheeler, Kang Chen, Nihar R. Nayak. VEGF may contribute to macrophage recruitment and M2 polarization in the decidua. PLoS ONE. 2018; 13 (1):e0191040.
Chicago/Turabian StyleKaren C. Wheeler; Manoj Jena; Bhola Pradhan; Neha Nayak; Subhendu Das; Chaur-Dong Hsu; David S. Wheeler; Kang Chen; Nihar R. Nayak. 2018. "VEGF may contribute to macrophage recruitment and M2 polarization in the decidua." PLoS ONE 13, no. 1: e0191040.
Mammary gland is a unique organ with its function of milk synthesis, secretion, and involution to prepare the gland for subsequent lactation. The mammary epithelial cells proliferate, differentiate, undergo apoptosis, and tissue remodeling following a cyclic pathway in lactation – involution – lactation cycle, thus fine tuning the molecular events through hormones, and regulatory molecules. Several studies are performed on the mammary gland development, lactogenesis, and involution process in molecular details. The developmental stages of mammary gland are embryonic, pre-pubertal, pubertal, pregnancy, lactation, and involution. Major developmental processes occur after puberty with hormones and growth factors playing crucial role. The two major pathways such as Janus kinases-signal transducer and activator of transcription pathway and PI3K-Akt pathway play a major role in maintaining the lactation. The involution process is a well-orchestrated event involving several signaling molecules and making the gland ready for subsequent lactation. The review focuses on findings with molecular details of different stages of the mammary gland development and signaling pathways involved in lactation–involution cycle. Deep insight into the developmental stages of mammary gland will pave the way to understand mammary gland biology, apoptosis, oncogenesis, and it will help the researchers to use mammary gland as a model for research on various aspects.
Manoj Kumar Jena; Ashok Kumar Mohanty. NEW INSIGHTS OF MAMMARY GLAND DURING DIFFERENT STAGES OF DEVELOPMENT. Asian Journal of Pharmaceutical and Clinical Research 2017, 10, 35 .
AMA StyleManoj Kumar Jena, Ashok Kumar Mohanty. NEW INSIGHTS OF MAMMARY GLAND DURING DIFFERENT STAGES OF DEVELOPMENT. Asian Journal of Pharmaceutical and Clinical Research. 2017; 10 (11):35.
Chicago/Turabian StyleManoj Kumar Jena; Ashok Kumar Mohanty. 2017. "NEW INSIGHTS OF MAMMARY GLAND DURING DIFFERENT STAGES OF DEVELOPMENT." Asian Journal of Pharmaceutical and Clinical Research 10, no. 11: 35.
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Manoj Kumar Jena; Soumya Ranjan Nayak. Post Mortem Study of Fatal Blunt Trauma to Chest. Medico-Legal Update 2017, 17, 160 .
AMA StyleManoj Kumar Jena, Soumya Ranjan Nayak. Post Mortem Study of Fatal Blunt Trauma to Chest. Medico-Legal Update. 2017; 17 (2):160.
Chicago/Turabian StyleManoj Kumar Jena; Soumya Ranjan Nayak. 2017. "Post Mortem Study of Fatal Blunt Trauma to Chest." Medico-Legal Update 17, no. 2: 160.
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Soumya Ranjan Nayak; Subal Kumar Naik; Ashok Kumar Samanta; Manoj Kumar Jena. Analysis of Profile of Hanging Deaths in Coastal Odisha. Journal of Indian Academy of Forensic Medicine 2017, 39, 16 .
AMA StyleSoumya Ranjan Nayak, Subal Kumar Naik, Ashok Kumar Samanta, Manoj Kumar Jena. Analysis of Profile of Hanging Deaths in Coastal Odisha. Journal of Indian Academy of Forensic Medicine. 2017; 39 (1):16.
Chicago/Turabian StyleSoumya Ranjan Nayak; Subal Kumar Naik; Ashok Kumar Samanta; Manoj Kumar Jena. 2017. "Analysis of Profile of Hanging Deaths in Coastal Odisha." Journal of Indian Academy of Forensic Medicine 39, no. 1: 16.