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Almudena Torres-Trenas
Departamento de Protección de Cultivos, Instituto de Agricultura Sostenible (IAS), Consejo Superior de Investigaciones Científicas (CSIC), 14004 Córdoba, Spain

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Journal article
Published: 03 March 2020 in Viruses
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A novel mycovirus named Fusarium oxysporum f. sp. dianthi mitovirus 1 (FodMV1) has been identified infecting a strain of Fusarium oxysporum f. sp. dianthi from Colombia. The genome of FodMV1 is 2313 nt long, and comprises a 172-nt 5’-UTR, a 2025-nt single ORF encoding an RdRp of 675 amino acid residues, and a 113-nt 3´-UTR. Homology BlastX searches identifies FodMV1 as a novel member of the genus Mitovirus in the family Narnaviridae. As the rest of mitoviruses, the genome of FodMV1 presents a high percentage of A+U (58.8%) and contains a number of UGA codons that encode the amino acid tryptophan rather than acting as stop codons as in the universal genetic code. Another common feature with other mitoviruses is that the 5′- and 3′-UTR regions of FodMV1 can be folded into potentially stable stem-loop structures. Result from phylogenetic analysis place FodMV1 in a different clade than the rest of mitoviruses described in other Fusarium spp. Incidence of FodMV1-infections in the collection of F. oxysporum f. sp. dianthi isolates analyzed is relatively high. Of particular interest is the fact that FodMV1 has been detected infecting isolates from two geographical areas as distant as Spain and Colombia.

ACS Style

Almudena Torres-Trenas; Encarnación Pérez-Artés. Characterization and Incidence of the First Member of the Genus Mitovirus Identified in the Phytopathogenic Species Fusarium oxysporum. Viruses 2020, 12, 279 .

AMA Style

Almudena Torres-Trenas, Encarnación Pérez-Artés. Characterization and Incidence of the First Member of the Genus Mitovirus Identified in the Phytopathogenic Species Fusarium oxysporum. Viruses. 2020; 12 (3):279.

Chicago/Turabian Style

Almudena Torres-Trenas; Encarnación Pérez-Artés. 2020. "Characterization and Incidence of the First Member of the Genus Mitovirus Identified in the Phytopathogenic Species Fusarium oxysporum." Viruses 12, no. 3: 279.

Original research article
Published: 24 January 2020 in Frontiers in Microbiology
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A novel mycovirus named Fusarium oxysporum f. sp. dianthi hypovirus 2 (FodHV2) has been identified infecting isolates Fod 408 and Fod 409 of Fusarium oxysporum f. sp. dianthi from Morocco. The genome of FodHV2 is 9,444 nucleotides long excluding the poly(A) tail, and has a single open reading frame encoding a polyprotein. The polyprotein contains three highly conserved domains of UDP glucose/sterol glucosyltransferase, RNA-dependent RNA polymerase, and viral RNA helicase. In addition, particular residues of Cys, Hys, and Gly detected in the N-terminal region suggest the presence of the catalytic site of a highly diverged papain-like protease. Genomic organization, presence of particular conserved motifs, and phylogenetic analyses based on multiple alignments clearly grouped FodHV2 with the members of the family Hypoviridae. FodHV2 was transferred by hyphal anastomosis to a recipient HygR-tagged virus-free strain. The comparison of the infected and non-infected isogenic strains showed that FodHV2 did not alter the vegetative growth, neither the conidiation nor the virulence of its fungal host. Efficiency of FodHV2 transmission through the conidia was 100% in both the original and the recipient infected-isolates. To the best of our knowledge, this is the first report of a hypovirus infecting the plant pathogen F. oxysporum, and also the first one of a hypovirus detected in a fungal strain from the African continent.

ACS Style

Almudena Torres-Trenas; M. Carmen Cañizares; M. Dolores García-Pedrajas; Encarnación Pérez-Artés. Molecular and Biological Characterization of the First Hypovirus Identified in Fusarium oxysporum. Frontiers in Microbiology 2020, 10, 3131 .

AMA Style

Almudena Torres-Trenas, M. Carmen Cañizares, M. Dolores García-Pedrajas, Encarnación Pérez-Artés. Molecular and Biological Characterization of the First Hypovirus Identified in Fusarium oxysporum. Frontiers in Microbiology. 2020; 10 ():3131.

Chicago/Turabian Style

Almudena Torres-Trenas; M. Carmen Cañizares; M. Dolores García-Pedrajas; Encarnación Pérez-Artés. 2020. "Molecular and Biological Characterization of the First Hypovirus Identified in Fusarium oxysporum." Frontiers in Microbiology 10, no. : 3131.

Original research article
Published: 12 March 2019 in Frontiers in Cellular and Infection Microbiology
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Mycoviruses that induce hypovirulence in phytopathogenic fungi are interesting because their potential use as biological control agents of the plant diseases caused by their fungal hosts. The recently identified chrysovirus Fusarium oxysporum f. sp. dianthi virus 1 (FodV1) has been associated to the induction of hypovirulence in Fusarium oxysporum f. sp. dianthi, the forma specialis of F. oxysporum that causes vascular wilt in carnation (Dianthus caryophyllus). In this work, we have used confocal laser scanner microscopy and two isogenic GFP-labeled strains of F. oxysporum f. sp. dianthi infected (V+) and not infected (V−) with the Fusarium oxysporum f. sp. dianthi virus 1, respectively, to analyze the effect of mycovirus FodV1 on the plant colonization pattern of its fungal host. Results demonstrate that FodV1-viral infection affects the speed and spatial distribution of fungal colonization into the plant. Initial stages of external root colonization were similar for both strains, but the virus-free strain colonized the internal plant tissues faster than the virus-infected strain. In addition, other differences related to the specific zone colonized and the density of colonization were observed between both F. oxysporum f. sp. dianthi strains. The hyphae of both V− and V+ strains progressed up through the xylem vessels but differences in the number of vessels colonized and of hyphae inside them were found. Moreover, as colonization progressed, V− and V+ hyphae propagated horizontally reaching the central medulla but, while the virus-free strain V− densely colonized the interior of the medulla cells, the virus-infected strain V+ appeared mainly in the intercellular spaces and with a lower density of colonization. Finally, the incidence of FodV1-viral infections in a collection of 221 isolates sampled between 2008 and 2012 in the geographic area where the originally infected isolate was obtained has been also analyzed. The very low (<2%) incidence of viral infections is discussed here. To the best of our knowledge, this work provides the first microscopic evidence about the effect of a hypovirulence-inducing mycovirus on the pattern of plant colonization by its fungal host.

ACS Style

Almudena Torres-Trenas; Pilar Prieto; M. Carmen Cañizares; Maria D. García-Pedrajas; Encarnación Pérez-Artés. Mycovirus Fusarium oxysporum f. sp. dianthi Virus 1 Decreases the Colonizing Efficiency of Its Fungal Host. Frontiers in Cellular and Infection Microbiology 2019, 9, 51 .

AMA Style

Almudena Torres-Trenas, Pilar Prieto, M. Carmen Cañizares, Maria D. García-Pedrajas, Encarnación Pérez-Artés. Mycovirus Fusarium oxysporum f. sp. dianthi Virus 1 Decreases the Colonizing Efficiency of Its Fungal Host. Frontiers in Cellular and Infection Microbiology. 2019; 9 ():51.

Chicago/Turabian Style

Almudena Torres-Trenas; Pilar Prieto; M. Carmen Cañizares; Maria D. García-Pedrajas; Encarnación Pérez-Artés. 2019. "Mycovirus Fusarium oxysporum f. sp. dianthi Virus 1 Decreases the Colonizing Efficiency of Its Fungal Host." Frontiers in Cellular and Infection Microbiology 9, no. : 51.

Journal article
Published: 05 May 2016 in Plant, Cell & Environment
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Purines are essential molecules formed in a highly regulated pathway in all organisms. In tropical legumes, the nitrogen fixed in the nodules is used to generate ureides through the oxidation of de novo synthesized purines. Glutamine phosphoribosyl pyrophosphate amidotransferase (PRAT) catalyzes the first committed step of de novo purine synthesis. In Phaseolus vulgaris there are three genes coding for PRAT. The three full-length sequences, which are intron-less genes, were cloned and their expression levels were determined under conditions that affect the synthesis of purines. One of the three genes, PvPRAT3, is highly expressed in nodules and protein amount and enzymatic activity in these tissues correlate with nitrogen fixation activity. Inhibition of PvPRAT3 gene expression by RNAi-silencing and subsequent metabolomic analysis of the transformed roots shows that PvPRAT3 is essential for the synthesis of ureides in P. vulgaris nodules. This article is protected by copyright. All rights reserved.

ACS Style

Inmaculada Coleto; Almudena Torres-Trenas; Alexander Erban; Joachim Kopka; Manuel Pineda Alamillo. Functional specialization of one copy of glutamine phosphoribosyl pyrophosphate amidotransferase in ureide production from symbiotically fixed nitrogen in Phaseolus vulgaris. Plant, Cell & Environment 2016, 39, 1767 -1779.

AMA Style

Inmaculada Coleto, Almudena Torres-Trenas, Alexander Erban, Joachim Kopka, Manuel Pineda Alamillo. Functional specialization of one copy of glutamine phosphoribosyl pyrophosphate amidotransferase in ureide production from symbiotically fixed nitrogen in Phaseolus vulgaris. Plant, Cell & Environment. 2016; 39 (8):1767-1779.

Chicago/Turabian Style

Inmaculada Coleto; Almudena Torres-Trenas; Alexander Erban; Joachim Kopka; Manuel Pineda Alamillo. 2016. "Functional specialization of one copy of glutamine phosphoribosyl pyrophosphate amidotransferase in ureide production from symbiotically fixed nitrogen in Phaseolus vulgaris." Plant, Cell & Environment 39, no. 8: 1767-1779.