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Dr. Sergio Lopez
Department of Cell Biology, Faculty of Biology, University of Seville, Seville, 41012, Spain

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0 Cell Signaling
0 Lipid Metabolism
0 Nutrition
0 Cardiovascular Diseases
0 Traffic membranes

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Research article
Published: 15 June 2021 in Molecular Nutrition & Food Research
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Scope The role of dietary fatty acids in the generation of bone marrow (BM) immune cells and their trafficking to extramedullary compartments in the obesity is not yet fully understood. Methods and Results C57BL/6J mice are randomly assigned to isocaloric high-fat diets (HFDs) formulate with dietary fats rich in saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs) or MUFAs fortified with eicosapentaenoic and docosahexaenoic acids for 20 weeks, followed by profiling of the obese metabolic phenotype and immunophenotypic features of immune cells in blood, spleen, and BM. All HFDs induce an obese phenotype, but it becomes largely less disruptive after the HFDs are enriched in MUFAs, which also induce signs of granulopoiesis and an expansion of long-term hematopoietic stem and granulocyte-macrophage progenitor cells in BM. In contrast, a HFD enriched in SFAs disturbs the fitness of medullary lymphocytes and promotes monopoiesis in favor of pro-inflammatory activated subsets. Conclusion The reshaping of the fatty acid pools with MUFAs from the diet serves to manipulate the generation and trafficking of immune cells that are biased during obesity. These findings reveal a novel strategy by which dietary MUFAs may be instrumental in combating HFD-induced dysfunctional immune systems.

ACS Style

Ana Lemus‐Conejo; Mayte Medrano; Sergio Lopez; Maria C. Millan‐Linares; Maria A. Rosillo; Jose A. Perez‐Simon; Francisco J. G. Muriana; Rocio Abia. MUFAs in High‐Fat Diets Protect against Obesity‐Induced Bias of Hematopoietic Cell Lineages. Molecular Nutrition & Food Research 2021, 65, 2001203 .

AMA Style

Ana Lemus‐Conejo, Mayte Medrano, Sergio Lopez, Maria C. Millan‐Linares, Maria A. Rosillo, Jose A. Perez‐Simon, Francisco J. G. Muriana, Rocio Abia. MUFAs in High‐Fat Diets Protect against Obesity‐Induced Bias of Hematopoietic Cell Lineages. Molecular Nutrition & Food Research. 2021; 65 (16):2001203.

Chicago/Turabian Style

Ana Lemus‐Conejo; Mayte Medrano; Sergio Lopez; Maria C. Millan‐Linares; Maria A. Rosillo; Jose A. Perez‐Simon; Francisco J. G. Muriana; Rocio Abia. 2021. "MUFAs in High‐Fat Diets Protect against Obesity‐Induced Bias of Hematopoietic Cell Lineages." Molecular Nutrition & Food Research 65, no. 16: 2001203.

Journal article
Published: 03 January 2021 in International Journal of Molecular Sciences
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Golgi trafficking depends on the small GTPase Arf1 which, upon activation, drives the assembly of different coats onto budding vesicles. Two related types of guanine nucleotide exchange factors (GEFs) activate Arf1 at different Golgi sites. In yeast, Gea1 in the cis-Golgi and Gea2 in the medial-Golgi activate Arf1 to form COPI-­coated vesicles for retrograde cargo sorting, whereas Sec7 generates clathrin/adaptor­-coated vesicles at the trans-Golgi network (TGN) for forward cargo transport. A central question is how the same activated Arf1 protein manages to assemble different coats depending on the donor Golgi compartment. A previous study has postulated that the interaction between Gea1 and COPI would channel Arf1 activation for COPI vesicle budding. Here, we found that the p24 complex, a major COPI vesicle cargo, promotes the binding of Gea1 with COPI by increasing the COPI association to the membrane independently of Arf1 activation. Furthermore, the p24 complex also facilitates the interaction of Arf1 with its COPI effector. Therefore, our study supports a mechanism by which the p24 complex contributes to program Arf1 activation by Gea1 for selective COPI coat assembly at the cis-Golgi compartment.

ACS Style

Susana Sabido-Bozo; Ana Maria Perez-Linero; Javier Manzano-Lopez; Sofia Rodriguez-Gallardo; Auxiliadora Aguilera-Romero; Alejandro Cortes-Gomez; Sergio Lopez; Ralf Erik Wellinger; Manuel Muñiz. The p24 Complex Contributes to Specify Arf1 for COPI Coat Selection. International Journal of Molecular Sciences 2021, 22, 423 .

AMA Style

Susana Sabido-Bozo, Ana Maria Perez-Linero, Javier Manzano-Lopez, Sofia Rodriguez-Gallardo, Auxiliadora Aguilera-Romero, Alejandro Cortes-Gomez, Sergio Lopez, Ralf Erik Wellinger, Manuel Muñiz. The p24 Complex Contributes to Specify Arf1 for COPI Coat Selection. International Journal of Molecular Sciences. 2021; 22 (1):423.

Chicago/Turabian Style

Susana Sabido-Bozo; Ana Maria Perez-Linero; Javier Manzano-Lopez; Sofia Rodriguez-Gallardo; Auxiliadora Aguilera-Romero; Alejandro Cortes-Gomez; Sergio Lopez; Ralf Erik Wellinger; Manuel Muñiz. 2021. "The p24 Complex Contributes to Specify Arf1 for COPI Coat Selection." International Journal of Molecular Sciences 22, no. 1: 423.

Research article
Published: 11 December 2020 in Science Advances
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Protein sorting in the secretory pathway is crucial to maintain cellular compartmentalization and homeostasis. In addition to coat-mediated sorting, the role of lipids in driving protein sorting during secretory transport is a longstanding fundamental question that still remains unanswered. Here, we conduct 3D simultaneous multicolor high-resolution live imaging to demonstrate in vivo that newly synthesized glycosylphosphatidylinositol-anchored proteins having a very long chain ceramide lipid moiety are clustered and sorted into specialized endoplasmic reticulum exit sites that are distinct from those used by transmembrane proteins. Furthermore, we show that the chain length of ceramide in the endoplasmic reticulum membrane is critical for this sorting selectivity. Our study provides the first direct in vivo evidence for lipid chain length–based protein cargo sorting into selective export sites of the secretory pathway.

ACS Style

Sofia Rodriguez-Gallardo; Kazuo Kurokawa; Susana Sabido-Bozo; Alejandro Cortes-Gomez; Atsuko Ikeda; Valeria Zoni; Auxiliadora Aguilera-Romero; Ana Maria Perez-Linero; Sergio Lopez; Miho Waga; Misako Araki; Miyako Nakano; Howard Riezman; Kouichi Funato; Stefano Vanni; Akihiko Nakano; Manuel Muñiz. Ceramide chain length–dependent protein sorting into selective endoplasmic reticulum exit sites. Science Advances 2020, 6, eaba8237 .

AMA Style

Sofia Rodriguez-Gallardo, Kazuo Kurokawa, Susana Sabido-Bozo, Alejandro Cortes-Gomez, Atsuko Ikeda, Valeria Zoni, Auxiliadora Aguilera-Romero, Ana Maria Perez-Linero, Sergio Lopez, Miho Waga, Misako Araki, Miyako Nakano, Howard Riezman, Kouichi Funato, Stefano Vanni, Akihiko Nakano, Manuel Muñiz. Ceramide chain length–dependent protein sorting into selective endoplasmic reticulum exit sites. Science Advances. 2020; 6 (50):eaba8237.

Chicago/Turabian Style

Sofia Rodriguez-Gallardo; Kazuo Kurokawa; Susana Sabido-Bozo; Alejandro Cortes-Gomez; Atsuko Ikeda; Valeria Zoni; Auxiliadora Aguilera-Romero; Ana Maria Perez-Linero; Sergio Lopez; Miho Waga; Misako Araki; Miyako Nakano; Howard Riezman; Kouichi Funato; Stefano Vanni; Akihiko Nakano; Manuel Muñiz. 2020. "Ceramide chain length–dependent protein sorting into selective endoplasmic reticulum exit sites." Science Advances 6, no. 50: eaba8237.

Journal article
Published: 25 August 2020 in International Journal of Environmental Research and Public Health
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Obesity is related to low-grade systemic inflammation. This state of inflammation is characterized by the alteration in adipokine regulation, which may lead to a situation of cardiometabolic risk. The aim of this study was to evaluate the effects of a concurrent training program on markers of lipoinflammation in adult people with obesity, comparing the response to the training between men and women. A quasi-experimental, quantitative, and longitudinal study with a pre–post intervention was conducted. An 8-week concurrent training program was carried out, in which 26 individuals with obesity participated (mean ± SD; age = 46.38 ± 4.66) (BMI = 36.05 ± 4.99) (12 men and 14 women). Before and after the intervention period, blood samples were taken by percutaneous puncture. The blood levels of adiponectin and leptin were evaluated. Significant differences were obtained in the adiponectin–leptin ratio (A/L ratio) of the entire sample (p = 0.009, ES = 0.53), which indicates a decrease in the risk of cardiovascular diseases and lipoinflammation. There were no significant differences in the improvements observed after the training in A/L ratio between women (A/L change = +63.5%) and men (A/L change= +59.2%). It can be concluded that the combination of aerobic exercise and resistance training induced an improvement in markers of lipoinflammation and cardiometabolic risk in the individuals with obesity evaluated in this study.

ACS Style

José Antonio González-Jurado; Walter Suárez-Carmona; Sergio López; Antonio Jesús Sánchez-Oliver. Changes in Lipoinflammation Markers in People with Obesity after a Concurrent Training Program: A Comparison between Men and Women. International Journal of Environmental Research and Public Health 2020, 17, 6168 .

AMA Style

José Antonio González-Jurado, Walter Suárez-Carmona, Sergio López, Antonio Jesús Sánchez-Oliver. Changes in Lipoinflammation Markers in People with Obesity after a Concurrent Training Program: A Comparison between Men and Women. International Journal of Environmental Research and Public Health. 2020; 17 (17):6168.

Chicago/Turabian Style

José Antonio González-Jurado; Walter Suárez-Carmona; Sergio López; Antonio Jesús Sánchez-Oliver. 2020. "Changes in Lipoinflammation Markers in People with Obesity after a Concurrent Training Program: A Comparison between Men and Women." International Journal of Environmental Research and Public Health 17, no. 17: 6168.

Journal article
Published: 22 May 2020 in Cells
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The cellular mechanisms that ensure the selectivity and fidelity of secretory cargo protein transport from the endoplasmic reticulum (ER) to the Golgi are still not well understood. The p24 protein complex acts as a specific cargo receptor for GPI-anchored proteins by facilitating their ER exit through a specialized export pathway in yeast. In parallel, the p24 complex can also exit the ER using the general pathway that exports the rest of secretory proteins with their respective cargo receptors. Here, we show biochemically that the p24 complex associates at the ER with other cargo receptors in a COPII-dependent manner, forming high-molecular weight multireceptor complexes. Furthermore, live cell imaging analysis reveals that the p24 complex is required to retain in the ER secretory cargos when their specific receptors are absent. This requirement does not involve neither the unfolded protein response nor the retrograde transport from the Golgi. Our results suggest that, in addition to its role as a cargo receptor in the specialized GPI-anchored protein pathway, the p24 complex also plays an independent role in secretory cargo selectivity during its exit through the general ER export pathway, preventing the non-selective bulk flow of native secretory cargos. This mechanism would ensure receptor-regulated cargo transport, providing an additional layer of regulation of secretory cargo selectivity during ER export.

ACS Style

Sergio Lopez; Ana Maria Perez-Linero; Javier Manzano-Lopez; Susana Sabido-Bozo; Alejandro Cortes-Gomez; Sofia Rodriguez-Gallardo; Auxiliadora Aguilera-Romero; Veit Goder; Manuel Muñiz. Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic Reticulum. Cells 2020, 9, 1295 .

AMA Style

Sergio Lopez, Ana Maria Perez-Linero, Javier Manzano-Lopez, Susana Sabido-Bozo, Alejandro Cortes-Gomez, Sofia Rodriguez-Gallardo, Auxiliadora Aguilera-Romero, Veit Goder, Manuel Muñiz. Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic Reticulum. Cells. 2020; 9 (5):1295.

Chicago/Turabian Style

Sergio Lopez; Ana Maria Perez-Linero; Javier Manzano-Lopez; Susana Sabido-Bozo; Alejandro Cortes-Gomez; Sofia Rodriguez-Gallardo; Auxiliadora Aguilera-Romero; Veit Goder; Manuel Muñiz. 2020. "Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic Reticulum." Cells 9, no. 5: 1295.

Review
Published: 17 July 2019 in International Journal of Molecular Sciences
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Protein export from the endoplasmic reticulum (ER) is an essential process in all eukaryotes driven by the cytosolic coat complex COPII, which forms vesicles at ER exit sites for transport of correctly assembled secretory cargo to the Golgi apparatus. The COPII machinery must adapt to the existing wide variety of different types of cargo proteins and to different cellular needs for cargo secretion. The study of the ER export of glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs), a special glycolipid-linked class of cell surface proteins, is contributing to address these key issues. Due to their special biophysical properties, GPI-APs use a specialized COPII machinery to be exported from the ER and their processing and maturation has been recently shown to actively regulate COPII function. In this review, we discuss the regulatory mechanisms by which GPI-APs are assembled and selectively exported from the ER.

ACS Style

Sergio Lopez; Sofia Rodriguez-Gallardo; Susana Sabido-Bozo; Manuel Muñiz. Endoplasmic Reticulum Export of GPI-Anchored Proteins. International Journal of Molecular Sciences 2019, 20, 3506 .

AMA Style

Sergio Lopez, Sofia Rodriguez-Gallardo, Susana Sabido-Bozo, Manuel Muñiz. Endoplasmic Reticulum Export of GPI-Anchored Proteins. International Journal of Molecular Sciences. 2019; 20 (14):3506.

Chicago/Turabian Style

Sergio Lopez; Sofia Rodriguez-Gallardo; Susana Sabido-Bozo; Manuel Muñiz. 2019. "Endoplasmic Reticulum Export of GPI-Anchored Proteins." International Journal of Molecular Sciences 20, no. 14: 3506.

Journal article
Published: 11 June 2019 in Energies
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Steam-explosion is a promising technology for recovering phenolic compounds from olive mill solid waste (OMSW) due to its high impact on the structure of the fibre. Moreover, the recovery of the phenols, which are well-known microbial inhibitors, could improve the subsequent biomethanization of the dephenolized OMSW to produce energy. However, there is a considerable lack of knowledge about how the remaining phenolic compounds could affect a long-term biomethanization process of steam-exploded OMSW. This work evaluated a semi-continuous mesophilic anaerobic digestion of dephenolized steam-exploited OMSW during a long operational period (275 days), assessing different organic loading rates (OLRs). The process was stable at an OLR of 1 gVS/(L·d), with a specific production rate of 163 ± 28 mL CH4/(gVS·d). However, the increment of the OLR up to 2 gVS/(L·d) resulted in total exhaust of the methane production. The increment in the propionic acid concentration up to 1486 mg/L could be the main responsible factor for the inhibition. Regardless of the OLR, the concentration of phenolic compounds was always lower than the inhibition limits. Therefore, steam-exploited OMSW could be a suitable substrate for anaerobic digestion at a suitable OLR.

ACS Style

Antonio Serrano; Fernando G. Fermoso; Bernabé Alonso-Fariñas; Guillermo Rodríguez-Gutiérrez; Sergio López; Juan Fernandez-Bolaños; Rafael Borja. Long-Term Evaluation of Mesophilic Semi-Continuous Anaerobic Digestion of Olive Mill Solid Waste Pretreated with Steam-Explosion. Energies 2019, 12, 2222 .

AMA Style

Antonio Serrano, Fernando G. Fermoso, Bernabé Alonso-Fariñas, Guillermo Rodríguez-Gutiérrez, Sergio López, Juan Fernandez-Bolaños, Rafael Borja. Long-Term Evaluation of Mesophilic Semi-Continuous Anaerobic Digestion of Olive Mill Solid Waste Pretreated with Steam-Explosion. Energies. 2019; 12 (11):2222.

Chicago/Turabian Style

Antonio Serrano; Fernando G. Fermoso; Bernabé Alonso-Fariñas; Guillermo Rodríguez-Gutiérrez; Sergio López; Juan Fernandez-Bolaños; Rafael Borja. 2019. "Long-Term Evaluation of Mesophilic Semi-Continuous Anaerobic Digestion of Olive Mill Solid Waste Pretreated with Steam-Explosion." Energies 12, no. 11: 2222.

Full paper
Published: 26 October 2018 in Chemistry & Biodiversity
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Colorectal cancer is the third most common cancer in the world. Many efforts have focused on finding natural molecules with potential chemo‐preventive activity due to their low toxicity compared to synthetic drugs. However, comprehensive information on the bioactive fractions and components is still missing. In this study, we developed a method for the quantitative separation and isolation of saponins from asparagus genotypes consisting of an adsorption chromatography and subsequent liquid chromatographic separation on a reversed‐phase column. The saponins isolated were tested for their cytotoxic activity against human colon cancer cell lines, which could develop cross‐resistance to a wide variety of chemotherapeutic drugs. Our results showed that Huétor‐Tájar asparagus saponins (HTSAP), mainly protodioscin and HTSAP‐10 have higher cytotoxic activity than HTSAP‐1, HTSAP‐6, and HTSAP‐8. This study links the potential anticancer effect of asparagus to specific saponins and unveils the triguero Huétor‐Tájar asparagus as a nutraceutical particularly in colon cancer therapies. This article is protected by copyright. All rights reserved.

ACS Style

Sara Jaramillo-Carmona; Rafael Guillén-Bejarano; Ana Jimenez-Araujo; Rocío Rodríguez Arcos; Sergio López. In Vitro Toxicity of Asparagus Saponins in Distinct Multidrug-Resistant Colon Cancer Cells. Chemistry & Biodiversity 2018, 15, e1800282 .

AMA Style

Sara Jaramillo-Carmona, Rafael Guillén-Bejarano, Ana Jimenez-Araujo, Rocío Rodríguez Arcos, Sergio López. In Vitro Toxicity of Asparagus Saponins in Distinct Multidrug-Resistant Colon Cancer Cells. Chemistry & Biodiversity. 2018; 15 (11):e1800282.

Chicago/Turabian Style

Sara Jaramillo-Carmona; Rafael Guillén-Bejarano; Ana Jimenez-Araujo; Rocío Rodríguez Arcos; Sergio López. 2018. "In Vitro Toxicity of Asparagus Saponins in Distinct Multidrug-Resistant Colon Cancer Cells." Chemistry & Biodiversity 15, no. 11: e1800282.

Journal article
Published: 01 July 2018 in The Journal of Nutritional Biochemistry
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The postprandial hypertriglyceridemia is an important and largely silent disturbance involved in the genesis of numerous pathological conditions. Exaggerated and prolonged states of postprandial hypertriglyceridemia are frequently related to the ingestion of meals enriched in saturated fatty acids (SFAs). MicroRNAs are non-coding RNAs that function as gene regulators and play significant roles in both health and disease. However, differential miRNA expression between fasting and postprandial states has never been elucidated. Here we studied the impact of a high-saturated fat meal, mainly rich in palmitic acid, on the miRNA signature in peripheral blood mononuclear cells (PBMCs) of nine male healthy individuals in the postprandial period by using a two-step analysis, miRNA array and validation through qRT-PCR. Compared with miRNA expression signature in PBMCs at fasting, 36 miRNAs were downregulated and 43 miRNAs were upregulated in PBMCs at postprandial hypertriglyceridemic peak. Six chromosomes (3, 7, 8, 12, 14, and 19) had nearly a half (48.1%) of dysregulated miRNA-gene-containing regions. Downregulated miR-300 and miR-369-3p and upregulated miR-495-3p, miR-129-5p, and miR-7-2-3p had the highest number of target genes. The differentially expressed miRNAs and their predicted target genes involved pathways in cancer, MAPK signalling pathway, endocytosis, and axon guidance. Only downregulated miRNAs notably targeted PI3K-Akt signalling pathways, whereas only upregulated miRNAs targeted focal adhesion, Wnt signalling pathway, transcriptional misregulation in cancer, and ubiquitin-mediated proteolysis. This is the first study of miRNA expression analysis of human PBMCs during postprandial hypertriglyceridemia and offers insight into new potential mechanisms by which dietary SFAs influence on health or disease.

ACS Style

Sergio Lopez; Beatriz Bermudez; Sergio Montserrat-De la Paz; Rocio Abia; Francisco J.G. Muriana. A microRNA expression signature of the postprandial state in response to a high-saturated-fat challenge. The Journal of Nutritional Biochemistry 2018, 57, 45 -55.

AMA Style

Sergio Lopez, Beatriz Bermudez, Sergio Montserrat-De la Paz, Rocio Abia, Francisco J.G. Muriana. A microRNA expression signature of the postprandial state in response to a high-saturated-fat challenge. The Journal of Nutritional Biochemistry. 2018; 57 ():45-55.

Chicago/Turabian Style

Sergio Lopez; Beatriz Bermudez; Sergio Montserrat-De la Paz; Rocio Abia; Francisco J.G. Muriana. 2018. "A microRNA expression signature of the postprandial state in response to a high-saturated-fat challenge." The Journal of Nutritional Biochemistry 57, no. : 45-55.

Research article
Published: 27 September 2017 in Journal of the Science of Food and Agriculture
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BACKGROUND The nature of dietary fats profoundly affects postprandial hypertriglyceridemia and glucose homeostasis. Niacin is a potent lipid‐lowering agent. However, limited data exist on postprandial triglycerides and glycemic control following co‐administration of high‐fat meals with a single dose of niacin in subjects with metabolic syndrome (MetS). The aim of the study was to explore whether a fat challenge containing predominantly saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs) or MUFAs plus omega‐3 long‐chain polyunsaturated (LCPUFAs) fatty acids together with a single dose of immediate‐release niacin have a relevant role in postprandial insulin and lipid status in subjects with MetS. RESULTS In a randomized crossover within‐subject design, 16 men with MetS were given a single dose of immediate‐release niacin (2 g) and ∼15 cal kg−1 body weight meals containing either SFAs, MUFAs, MUFAs plus omega‐3 LCPUFAs or no fat. At baseline and hourly over 6 h, plasma glucose, insulin, C‐peptide, triglycerides, free fatty acids (FFAs), total cholesterol, and both high‐ and low‐density lipoprotein cholesterol were assessed. Co‐administered with niacin, high‐fat meals significantly increased the postprandial concentrations of glucose, insulin, C‐peptide, triglycerides, FFAs and postprandial indices of β‐cell function. However, postprandial indices of insulin sensitivity were significantly decreased. These effects were significantly attenuated with MUFAs or MUFAs plus omega‐3 LCPUFAs when compared with SFAs. CONCLUSION In the setting of niacin co‐administration and compared to dietary SFAs, MUFAs limit the postprandial insulin, triglyceride and FFA excursions, and improve postprandial glucose homeostasis in MetS. © 2017 Society of Chemical Industry

ACS Style

Sergio Montserrat-De La Paz; Sergio Lopez; Beatriz Bermudez; Juan M Guerrero; Rocio Abia; Francisco Jg Muriana. Effects of immediate-release niacin and dietary fatty acids on acute insulin and lipid status in individuals with metabolic syndrome. Journal of the Science of Food and Agriculture 2017, 98, 2194 -2200.

AMA Style

Sergio Montserrat-De La Paz, Sergio Lopez, Beatriz Bermudez, Juan M Guerrero, Rocio Abia, Francisco Jg Muriana. Effects of immediate-release niacin and dietary fatty acids on acute insulin and lipid status in individuals with metabolic syndrome. Journal of the Science of Food and Agriculture. 2017; 98 (6):2194-2200.

Chicago/Turabian Style

Sergio Montserrat-De La Paz; Sergio Lopez; Beatriz Bermudez; Juan M Guerrero; Rocio Abia; Francisco Jg Muriana. 2017. "Effects of immediate-release niacin and dietary fatty acids on acute insulin and lipid status in individuals with metabolic syndrome." Journal of the Science of Food and Agriculture 98, no. 6: 2194-2200.

Journals
Published: 04 July 2017 in Food & Function
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Novel biological activities for tyrosol metabolites on human endothelial cells.

ACS Style

Francisco J. G. Muriana; Sergio Montserrat-De la Paz; Ricardo Lucas; Beatriz Bermudez; Sara Jaramillo; Juan C. Morales; Rocio Abia; Sergio Lopez. Tyrosol and its metabolites as antioxidative and anti-inflammatory molecules in human endothelial cells. Food & Function 2017, 8, 2905 -2914.

AMA Style

Francisco J. G. Muriana, Sergio Montserrat-De la Paz, Ricardo Lucas, Beatriz Bermudez, Sara Jaramillo, Juan C. Morales, Rocio Abia, Sergio Lopez. Tyrosol and its metabolites as antioxidative and anti-inflammatory molecules in human endothelial cells. Food & Function. 2017; 8 (8):2905-2914.

Chicago/Turabian Style

Francisco J. G. Muriana; Sergio Montserrat-De la Paz; Ricardo Lucas; Beatriz Bermudez; Sara Jaramillo; Juan C. Morales; Rocio Abia; Sergio Lopez. 2017. "Tyrosol and its metabolites as antioxidative and anti-inflammatory molecules in human endothelial cells." Food & Function 8, no. 8: 2905-2914.

Book chapter
Published: 21 June 2017 in Fatty Acids
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ACS Style

Sergio Montserrat‐De La Paz; Rocio Abia; Beatriz Bermudez; Sergio Lopez; Francisco Jg Muriana. Fatty Acids on Osteoclastogenesis. Fatty Acids 2017, 1 .

AMA Style

Sergio Montserrat‐De La Paz, Rocio Abia, Beatriz Bermudez, Sergio Lopez, Francisco Jg Muriana. Fatty Acids on Osteoclastogenesis. Fatty Acids. 2017; ():1.

Chicago/Turabian Style

Sergio Montserrat‐De La Paz; Rocio Abia; Beatriz Bermudez; Sergio Lopez; Francisco Jg Muriana. 2017. "Fatty Acids on Osteoclastogenesis." Fatty Acids , no. : 1.

Journal article
Published: 05 June 2017 in Grasas y Aceites
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Age-related macular degeneration (AMD) is a medical condition of central loss vision and blindness. Numerous studies have revealed that changes on certain dietary fatty acids (FAs) could have useful for AMD management. This review summarizes the effects of dietary omega-3 long-chain PUFAs, MUFAs, and SFAs, and lipoproteins on AMD. Findings are consistent with the beneficial role of dietary omega-3 long-chain PUFAs, while the effects of dietary MUFAs and SFAs appeared to be ambiguous with respect to the possible protection from MUFAs and to the possible adverse impact from SFAs on AMD. Some of the pathological mechanisms associated with lipoproteins on AMD share those observed previously in cardiovascular diseases. It was also noticed that the effects of FAs in the diet and lipoprotein on AMD could be modulated by genetic variants. From a population health perspective, the findings of this review are in favour of omega-3 long-chain FAs recommendations in a preventive and therapeutic regimen to attain lower AMD occurrence and progression rates. Additional long-term and short-term nutrigenomic studies are required to clearly establish the role and the relevance of interaction of dietary FAs, lipoproteins, and genes in the genesis and progression of AMD.

ACS Style

S. Montserrat-De La Paz; Mc Naranjo; Beatriz Bermudez; S. López; R. Abia; F. J.G. Muriana. Dietary fatty acids and lipoproteins on progression of age-related macular degeneration. Grasas y Aceites 2017, 68, 187 .

AMA Style

S. Montserrat-De La Paz, Mc Naranjo, Beatriz Bermudez, S. López, R. Abia, F. J.G. Muriana. Dietary fatty acids and lipoproteins on progression of age-related macular degeneration. Grasas y Aceites. 2017; 68 (2):187.

Chicago/Turabian Style

S. Montserrat-De La Paz; Mc Naranjo; Beatriz Bermudez; S. López; R. Abia; F. J.G. Muriana. 2017. "Dietary fatty acids and lipoproteins on progression of age-related macular degeneration." Grasas y Aceites 68, no. 2: 187.

Article
Published: 28 April 2017 in Molecular Nutrition & Food Research
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Postprandial triglyceride-rich lipoproteins (TRLs) promote atherosclerosis. Recent research points the bone marrow (BM) as a primary site in atherosclerosis. We elucidated how the acute administration of monounsaturated fatty acids (MUFAs) MUFAs, omega-3 polyunsaturated fatty acids (PUFAs) PUFAs and saturated fatty acids (SFAs) affects human circulating and murine BM neutrophil lipid accumulation and functionality. Postprandial hypertriglyceridemia was induced in healthy subjects and Apoe−/− mice by the acute administration of dietary fats enriched in MUFAs, PUFAs, or SFAs. Postprandial hypertriglyceridemia increased apolipoprotein-B48 receptor (ApoB48R) transcriptional activity that was linearly correlated with intracellular triglycerides (TGs) TGs accumulation in human circulating and murine BM neutrophils. MUFA and omega-3 PUFAs attenuated ApoB48R gene expression and intracellular TG accumulation compared to SFAs. TRLs induced apoB48R-dependent TG accumulation in human neutrophils ex vivo. Murine BM neutrophils showed a decrease in surface L-selectin and an increase in TNF-α and IL-1β mRNA expressions only after SFAs administration. TRLs enriched in SFAs induced BM neutrophil degranulation ex vivo suggesting cell priming/activation. Postprandial TRLs disrupts the normal biology and function of circulating and BM neutrophils. MUFA- and omega-3 PUFA-rich dietary fats such as virgin olive oil or fish oil has the potential to prevent excessive neutrophil lipid accumulation and activation by targeting the fatty acid composition of TRLs.

ACS Style

Almudena Ortega-Gomez; Lourdes M. Varela; Sergio Lopez; Sergio Montserrat-De la Paz; Rosario Sánchez; Francisco J.G. Muriana; Beatriz Bermúdez; Rocío Abia. Postprandial triglyceride-rich lipoproteins promote lipid accumulation and apolipoprotein B-48 receptor transcriptional activity in human circulating and murine bone marrow neutrophils in a fatty acid-dependent manner. Molecular Nutrition & Food Research 2017, 61, 1 .

AMA Style

Almudena Ortega-Gomez, Lourdes M. Varela, Sergio Lopez, Sergio Montserrat-De la Paz, Rosario Sánchez, Francisco J.G. Muriana, Beatriz Bermúdez, Rocío Abia. Postprandial triglyceride-rich lipoproteins promote lipid accumulation and apolipoprotein B-48 receptor transcriptional activity in human circulating and murine bone marrow neutrophils in a fatty acid-dependent manner. Molecular Nutrition & Food Research. 2017; 61 (9):1.

Chicago/Turabian Style

Almudena Ortega-Gomez; Lourdes M. Varela; Sergio Lopez; Sergio Montserrat-De la Paz; Rosario Sánchez; Francisco J.G. Muriana; Beatriz Bermúdez; Rocío Abia. 2017. "Postprandial triglyceride-rich lipoproteins promote lipid accumulation and apolipoprotein B-48 receptor transcriptional activity in human circulating and murine bone marrow neutrophils in a fatty acid-dependent manner." Molecular Nutrition & Food Research 61, no. 9: 1.

Clinical trial
Published: 27 March 2017 in Molecular Nutrition & Food Research
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ScopeMacrophage plasticity allows adapting to different environments, having a dual activity in inflammatory-related diseases. Our hypothesis is that the type of dietary fatty acids into human postprandial triglyceride-rich lipoproteins (TRLs), alone or in combination with niacin (vitamin B3), could modulate the plasticity of monocytes-macrophages.Methods and resultsWe isolated TRLs at the postprandial peak from blood samples of healthy volunteers after the ingestion of a meal rich in saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs) or MUFAs plus omega-3 long-chain polyunsaturated fatty acids (LCPUFAs). Autologous monocytes isolated at fasting were first induced to differentiate into naïve macrophages. We observed that postprandial TRL-MUFAs, particularly in combination with niacin, enhance competence to monocytes to differentiate and polarise into M2 macrophages. Postprandial TRL-SFAs made polarised macrophages prone to an M1 phenotype. In contrast to dietary SFAs, dietary MUFAs in the meals plus immediate-release niacin primed circulating monocytes for a reduced postprandial pro-inflammatory profile.ConclusionOur study underlines a role of postprandial TRLs as a metabolic entity in regulating the plasticity of the monocyte-macrophage lineage and also brings an understanding of the mechanisms by which dietary fatty acids are environmental factors fostering the innate immune responsiveness in humans.

ACS Style

Sergio Montserrat-De La Paz; Dolores Rodriguez; Magdalena P. Cardelo; Maria C. Naranjo; Beatriz Bermudez; Rocio Abia; Francisco J.G. Muriana; Sergio Lopez. The effects of exogenous fatty acids and niacin on human monocyte-macrophage plasticity. Molecular Nutrition & Food Research 2017, 61, 1600824 .

AMA Style

Sergio Montserrat-De La Paz, Dolores Rodriguez, Magdalena P. Cardelo, Maria C. Naranjo, Beatriz Bermudez, Rocio Abia, Francisco J.G. Muriana, Sergio Lopez. The effects of exogenous fatty acids and niacin on human monocyte-macrophage plasticity. Molecular Nutrition & Food Research. 2017; 61 (8):1600824.

Chicago/Turabian Style

Sergio Montserrat-De La Paz; Dolores Rodriguez; Magdalena P. Cardelo; Maria C. Naranjo; Beatriz Bermudez; Rocio Abia; Francisco J.G. Muriana; Sergio Lopez. 2017. "The effects of exogenous fatty acids and niacin on human monocyte-macrophage plasticity." Molecular Nutrition & Food Research 61, no. 8: 1600824.

Journals
Published: 24 February 2017 in Food & Function
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Metabolic syndrome (MetS) is associated with obesity, dyslipidemia, type 2 diabetes, and chronic low-grade inflammation. Metabolic syndrome (MetS) is associated with obesity, dyslipidemia, type 2 diabetes, and chronic low-grade inflammation. The aim of this study was to determine the role of high-fat low-cholesterol diets (HFLCDs) rich in SFAs (HFLCD-SFAs), MUFAs (HFLCD-MUFAs) or MUFAs plus omega-3 long-chain PUFAs (HFLCD-PUFAs) on vascular calcification by the modulation of the RANKL/RANK/OPG system in the aortic roots of Lep ob/ob LDLR −/− mice. Animals fed with HFLCD-SFAs had increased weight and a greater atheroma plaque size, calcification, and RANKL/CATHK expression in the aortic root than mice on MUFA-enriched diets, with an increasing OPG expression in the aortic roots of the latter. Our study demonstrates that compared to dietary SFAs, MUFAs from olive oil protect against atherosclerosis by interfering with vascular calcification via the RANKL/RANK/OPG system in the setting of MetS. These findings open opportunities for developing novel nutritional strategies with olive oil as the most important dietary source of MUFAs (notably oleic acid) to prevent cardiovascular complications in MetS.

ACS Style

Maria C. Naranjo; Beatriz Bermudez; Indara Garcia; Sergio Lopez; Rocio Abia; Francisco J. G. Muriana; Sergio Montserrat-De La Paz. Dietary fatty acids on aortic root calcification in mice with metabolic syndrome. Food & Function 2017, 8, 1468 -1474.

AMA Style

Maria C. Naranjo, Beatriz Bermudez, Indara Garcia, Sergio Lopez, Rocio Abia, Francisco J. G. Muriana, Sergio Montserrat-De La Paz. Dietary fatty acids on aortic root calcification in mice with metabolic syndrome. Food & Function. 2017; 8 (4):1468-1474.

Chicago/Turabian Style

Maria C. Naranjo; Beatriz Bermudez; Indara Garcia; Sergio Lopez; Rocio Abia; Francisco J. G. Muriana; Sergio Montserrat-De La Paz. 2017. "Dietary fatty acids on aortic root calcification in mice with metabolic syndrome." Food & Function 8, no. 4: 1468-1474.

Journal article
Published: 02 February 2017 in Journal of Food Science
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The aim of this work was to study the saponin profiles from spears of different wild asparagus species in the context of its genetic diversity aside from geographical seed origin. They included Asparagus pseudoscaber Grecescu, Asparagus maritimus (L.) Mill., Asparagus brachiphyllus Turcz., Asparagus prostrates Dumort., and Asparagus officinalis L. The saponin analysis by LC-MS has shown that saponin profile from wild asparagus is similar to that previously described for triguero asparagus from Huétor-Tájar landrace (triguero HT), which had not ever been reported in the edible part of asparagus. All the samples, except A. officinalis, were characterized for having saponins distinct to protodioscin and the total saponin contents were 10-fold higher than those described for commercial hybrids of green asparagus. In particular, A. maritimus from different origins were rich in saponins previously found in triguero HT. These findings supported previous suggestion, based on genetic analysis, about A. maritimus being the origin of triguero HT. Multivariate statistics including principal component analysis and hierarchical clustering analysis were used to define both similarities and differences among samples. The results showed that the greatest variance of the tested wild asparagus could be attributed to differences in the concentration of particular saponins and this knowledge could be a tool for identifying similar species.

ACS Style

Sara Jaramillo‐Carmona; Rocío Rodriguez‐Arcos; Ana Jimenez-Araujo; Sergio López; Juan Gil; Roberto Moreno; Rafael Guillén‐Bejarano. Saponin Profile of Wild Asparagus Species. Journal of Food Science 2017, 82, 638 -646.

AMA Style

Sara Jaramillo‐Carmona, Rocío Rodriguez‐Arcos, Ana Jimenez-Araujo, Sergio López, Juan Gil, Roberto Moreno, Rafael Guillén‐Bejarano. Saponin Profile of Wild Asparagus Species. Journal of Food Science. 2017; 82 (3):638-646.

Chicago/Turabian Style

Sara Jaramillo‐Carmona; Rocío Rodriguez‐Arcos; Ana Jimenez-Araujo; Sergio López; Juan Gil; Roberto Moreno; Rafael Guillén‐Bejarano. 2017. "Saponin Profile of Wild Asparagus Species." Journal of Food Science 82, no. 3: 638-646.

Journal article
Published: 01 February 2017 in Journal of Functional Foods
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50 Páginas; 3 Scheme; 5 FigurasThe effects of chemically synthesized metabolites (sulfate and glucuronate forms) from hydroxytyrosol (HTyr) on oxidative stress and inflammation were investigated in TNF-α-activated human endothelial cells. HTyr sulfate metabolites decreased reactive oxygen species and prevented the decrease in glutathione, glutathione peroxidase 1, and glutamate-cysteine ligase catalytic subunit and up-regulated heme oxygenase-1 levels. HTyr and all tested HTyr metabolites (HTyr sulfate > HTyr glucuronate > HTyr) suppressed the phosphorylation of nuclear factor kappa B proteins, the gene expression of intercellular and vascular adhesion molecules, E-selectin, chemokine (Csingle bondC) motif ligand 2, and prostaglandin-endoperoxidase synthase 2 and the adhesion of human monocytes. In addition, HTyr sulfate metabolites suppressed plantar and ear swelling and myeloperoxidase activity in inflamed ear tissue in mice treated with carrageenan or 12-O-tetradecanoylphorbol-13-acetate. This study demonstrates the antioxidant and/or anti-inflammatory properties of HTyr metabolites in TNF-α-activated hECs and in the prevention of acute and chronic inflammation in mice.This study was funded by research grant P09-CVI-5007 (Junta de Andalucía, Spain). Contracts for R.L. and Angela Palma Pacheco (technician) were also supported by P09-CVI-5007. S.M. has the benefit of a FPI fellowship (BES-2012-056104) of MICINN. S.L. acknowledges a contract cofunded by the European Social Fund (ESF, Belgium) from the Spanish MINECO (JCI-2012-13084, Juan de la Cierva) and the Spanish Research Council (CSIC)/JAEdoc Program (JAEDOC089). B.B. acknowledges funds from the “V Own Research Plan” (University of Seville).Peer reviewe

ACS Style

Sergio Lopez; Sergio Montserrat-De la Paz; Ricardo Lucas; Beatriz Bermudez; Rocio Abia; Juan C. Morales; Francisco J.G. Muriana. Effect of metabolites of hydroxytyrosol on protection against oxidative stress and inflammation in human endothelial cells. Journal of Functional Foods 2017, 29, 238 -247.

AMA Style

Sergio Lopez, Sergio Montserrat-De la Paz, Ricardo Lucas, Beatriz Bermudez, Rocio Abia, Juan C. Morales, Francisco J.G. Muriana. Effect of metabolites of hydroxytyrosol on protection against oxidative stress and inflammation in human endothelial cells. Journal of Functional Foods. 2017; 29 ():238-247.

Chicago/Turabian Style

Sergio Lopez; Sergio Montserrat-De la Paz; Ricardo Lucas; Beatriz Bermudez; Rocio Abia; Juan C. Morales; Francisco J.G. Muriana. 2017. "Effect of metabolites of hydroxytyrosol on protection against oxidative stress and inflammation in human endothelial cells." Journal of Functional Foods 29, no. : 238-247.

Journal article
Published: 01 January 2017 in The Journal of Nutritional Biochemistry
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Niacin activates HCA receptor that results in the release of PGD. However, little is known on PGD-producing cells and the role of fatty acids. Notably M-CSF macrophages exhibited a timely dependent PGD production upon niacin challenge. Short pretreatment of M-CSF macrophages with autologous postprandial TRLs induced the down-regulation of HCA gene and up-regulation of genes encoding COX1 and COX2 enzymes in a fatty acid-dependent manner. These effects were paralleled by a higher PGD production with postprandial TRL-SFAs. The niacin-mediated transcriptional activity of all genes involved in PGD biosynthesis was desensitized in a time-dependent manner by postprandial TRLs, leading to a decreased PGD release. In vivo, the net excursions of PGD in plasma followed similar fatty acid-dependent patterns as those found for PGD release in vitro. The predominant fatty acid class in the diet acutely modulates PGD biosynthetic pathway both in vitro and in vivo.

ACS Style

Sergio Montserrat-De La Paz; Beatriz Bermudez; Sergio Lopez; Maria C. Naranjo; Yolanda Romero; Maria J. Bando-Hidalgo; Rocio Abia; Francisco J.G. Muriana. Exogenous fatty acids and niacin on acute prostaglandin D 2 production in human myeloid cells. The Journal of Nutritional Biochemistry 2017, 39, 22 -31.

AMA Style

Sergio Montserrat-De La Paz, Beatriz Bermudez, Sergio Lopez, Maria C. Naranjo, Yolanda Romero, Maria J. Bando-Hidalgo, Rocio Abia, Francisco J.G. Muriana. Exogenous fatty acids and niacin on acute prostaglandin D 2 production in human myeloid cells. The Journal of Nutritional Biochemistry. 2017; 39 ():22-31.

Chicago/Turabian Style

Sergio Montserrat-De La Paz; Beatriz Bermudez; Sergio Lopez; Maria C. Naranjo; Yolanda Romero; Maria J. Bando-Hidalgo; Rocio Abia; Francisco J.G. Muriana. 2017. "Exogenous fatty acids and niacin on acute prostaglandin D 2 production in human myeloid cells." The Journal of Nutritional Biochemistry 39, no. : 22-31.

Journal article
Published: 01 January 2017 in The Journal of Nutritional Biochemistry
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Niacin is a broad-spectrum lipid-regulating drug used for clinical therapy of chronic high-grade inflammatory diseases. However, the mechanisms by which either niacin or the byproducts of its catabolism ameliorate these inflammatory diseases are not clear yet. Human circulating monocytes and mature macrophages were used to analyze the effects of niacin and its metabolites (NAM, NUA and 2-Pyr) on oxidative stress, plasticity and inflammatory response by using biochemical, flow cytometry, quantitative real-time PCR and Western blot technologies. Niacin, NAM and 2-Pyr significantly decreased ROS, NO and NOS2 expression in LPS-treated human mature macrophages. Niacin and NAM skewed macrophage polarization toward antiinflammatory M2 macrophage whereas a trend toward proinflammatory M1 macrophage was noted following treatment with NUA. Niacin and NAM also reduced the inflammatory competence of LPS-treated human mature macrophages and promoted bias toward antiinflammatory CD14CD16 nonclassical human primary monocytes. This study reveals for the first time that niacin and its metabolites possess antioxidant, reprogramming and antiinflammatory properties on human primary monocytes and monocyte-derived macrophages. Our findings imply a new understanding of the mechanisms by which niacin and its metabolites favor a continuous and gradual plasticity process in the human monocyte/macrophage system.

ACS Style

Sergio Montserrat-De la Paz; M. Carmen Naranjo; Sergio Lopez; Rocio Abia; Francisco J. Garcia Muriana; Beatriz Bermudez. Niacin and its metabolites as master regulators of macrophage activation. The Journal of Nutritional Biochemistry 2017, 39, 40 -47.

AMA Style

Sergio Montserrat-De la Paz, M. Carmen Naranjo, Sergio Lopez, Rocio Abia, Francisco J. Garcia Muriana, Beatriz Bermudez. Niacin and its metabolites as master regulators of macrophage activation. The Journal of Nutritional Biochemistry. 2017; 39 ():40-47.

Chicago/Turabian Style

Sergio Montserrat-De la Paz; M. Carmen Naranjo; Sergio Lopez; Rocio Abia; Francisco J. Garcia Muriana; Beatriz Bermudez. 2017. "Niacin and its metabolites as master regulators of macrophage activation." The Journal of Nutritional Biochemistry 39, no. : 40-47.