3D-printing technologies for polymer formulations are experiencing huge development due to the complex and specific geometries that can be created employing additive manufacturing. However, to ensure sustainable growth, it is essential to replace the currently used fossil-based resins with new bio-sourced alternatives. In this study, tunable bis-allylated compounds were efficiently synthesized from cellulose-derived levoglucosenone via a chemo-enzymatic pathway whose greenness has been assessed by E-factor and Eco-Scale. Subsequently, photocuring was employed as an eco-friendly process to polymerize these compounds with a trifunctional thiol using thiol–ene click chemistry. Real-time techniques such as FT-IR, photo-DSC, and photo-rheology were employed to extensively characterize the UV process. The resulting thermosets were also subjected to a thorough analysis, covering their thermo-mechanical properties and degradation. Owing to the exceptional reactivity of this thiol–ene system, 3-D printed materials were achieved with remarkable precision.

When working on the synthesis of substituted cyclodextrins (CDs), the main challenge remains the analysis of the reaction media content. Our objective in this study was to fully characterise a complex isomers mixture of Lipidyl-βCDs (LipβCD) obtained with a degree of substitution 1 (DS = 1) from a one-step synthesis pathway. The benefit of tandem mass spectrometry (MS/MS) and ion mobility separation hyphenated with mass spectrometry (IM-MS) was investigated. The MS/MS fragment ion‘s relative intensities were analysed by principal component analysis (PCA) to discriminate isomers. The arrival time distribution (ATD) of each isomer was recorded using a travelling wave ion mobility (TWIM) cell allowing the determination of their respective experimental collision cross section (CCS_exp). The comparison with the predicted theoretical CCS (CCS_th) obtained from theoretical calculations propose a regioisomer assignment according to the βCD hydroxyl position (2, 3, or 6) involved in the reaction. These results were validated by extensive NMR structural analyses of pure isomers combined with molecular dynamics simulations. This innovative approach seems to be a promising tool to elucidate complex isomer mixtures such as substituted cyclodextrin derivatives.

It is not unusual for naturally occurring compounds to be limited for their use in cosmetics due to their low water solubility. Recently, aiming at accessing novel phlorizin (a glycosylated bioactive recovered from apple tree wood and already used in cosmetics as antioxidant ingredient) analogues, we reported the synthesis of very promising – but low water‐soluble – biomass‐derived chalcones (CHs) and dihydrochalcones (DHCs) exhibiting antioxidant and anti‐tyrosinase activities. Glycosylating bioactive compounds being one of the most common strategies to increase their water solubility, herein we report the enzymatic glycosylation of the CHs mentioned above, as well as DHC using cyclodextrin glycosyltransferases (CGTase), enzymes well‐known for catalyzing the selective α(1→4) transglycosylation. Indeed, while most natural glycosides are β‐glycosides (such as phlorizin), the selected enzyme produces selectively new α‐glycosides, thus expanding their structural diversity. A first step of separation using Centrifugal Partition Chromatography (CPC) led to mono‐, di‐ or triglycosides‐enriched fractions, which were then submitted to a comprehensive purification strategy for an in‐depth chemical profiling of the synthesized α‐glycosides, revealing that the major compounds were glycosylpyranosides. Surprisingly, among the diglycosides characterized, besides the expected maltoside compounds, nigeroside derivatives were also identified in significant amounts, depending on the starting compound structure. Finally, evaluating the antiradical, anti‐tyrosinase and antimicrobial activities of the major glycosides revealed them as potential sustainable alternatives to current petro‐sourced cosmetic ingredients.

This study proposes a sustainable strategy to valorise strawberry lignocellulosic agro-industrial byproducts through the recovery of antioxidant and antimicrobial compounds (AOM) for use in active edible coatings. Subcritical water extraction (SWE), optimised using response surface methodology, was applied to maximise phenolic content and antioxidant capacity while minimising sugars’ co-extraction. Optimal SWE conditions (120 °C, 5 min, and S/L ratio 40) yielded a total phenolic content (TPC) of 146.9 mg GAE/g DM and an antioxidant activity of 24.8 mg TE/g DM, comparable to ethanolic reflux extraction (138.4 mg GAE/g DM and 23.4 mg TE/g DM). Scale-up in a Parr pressurised reactor achieved 91.2% polyphenol recovery relative to accelerated solvent extraction (ASE). Purification using Amberlite^® XAD 7 resin enhanced TPC purity and antioxidant activity more than 2.5-fold, producing a desorbed fraction with a polyphenol purity of 93.9% (w/w, dry basis) and no detectable sugars. The purified AOM was incorporated (1% w/v) into a 1.5% (w) chitosan solution obtained from Hermetia illucens pupal exuviae to produce a biopolymeric active coating. Application to strawberries was associated with a reduction in fungal infection severity (−72%) and incidence (−66.7%) under natural infection conditions. Although fruit firmness declined during storage, coated samples showed significantly better firmness retention. These results demonstrate the effectiveness of combining chitosan with phenolic extracts obtained by SWE to enhance microbial stability and maintain fruit quality.