Tomato (Solanum lycopersicum L.) is one of the most important crops worldwide as per its production and the surface cultivated. The use of biostimulant products plays a fundamental role in mitigating the negative effects of climate change and reducing the use of conventional fertilizers. Many of these products are formulated with amino acids (AAs). This study was conducted to elucidate the effects of the foliar application of tyrosine (Tyr) (15 mM), lysine (Lys) (15 mM), methionine (Met) (15 mM), and a Tyr + Lys + Met (15 mM + 15 mM + 15 mM) mixture on the physiological and metabolic processes, vegetative growth, and nutritional state of Optima variety tomato plants. The results showed that application of the AAs, individually and combined, was beneficial for the growth of the aerial part, net assimilation of CO₂, and water use efficiency (WUE). Application of Tyr resulted in the best WUE. The metabolomics study revealed that AA treatments increased the concentration of proline, fructose, and glucose, whose role was to stimulate glycolysis and the Krebs cycle. Thus, the plants could have greater reduction power and energy, as well as more carbon molecules for their growth processes.

To ensure good fertilization, it is necessary to know the optimum nutrient levels for each crop. The most common method for obtaining this information for almond trees is to perform a foliar analysis coupled with the use of interpretive tools such as the traditional range of normality. However, currently, there are other, more sophisticated methods such as the DRIS (Diagnosis and Recommendation Integrated System) and the CND (Compositional Nutrient Diagnosis) which take into account the relationship between nutrients. However, little information is available with respect to these methods in the case of almond trees. In the present work, 288 samples of three contrasting varieties of almond were analyzed—Ferraduel, Ferragnes, and Garrigues (Prunus dulcis, Mill.)—corresponding to bi-weekly sampling between the months of May and September. Leaf analysis data, run with different mathematical and statistical models, lead to knowledge of the optimum period for harvesting samples and the determination of the ranges of normality and norms of DRIS and CND for the Ferraduel, Ferragnes, and Garrigues varieties. Data gained from the leaf nutrient content reported that the best season to harvest and interpret leaf samples was July. In addition, Ferraduel and Ferragnes had higher N, P, and K (2.22, 0.14, and 1.04 mg Kg⁻¹ dw, respectively) than Garrigues (2.00, 0.09. 0.67 mg Kg⁻¹ dw). The norms obtained with the leaf mineral data showed similar values between the Ferraduel and Ferragnes varieties but different values for Garrigues variety. Therefore, Garriges had the highest N/P, N/K, P/K, and P × Mg norms in the DRIS method and the highest V_N and V_Ca norms in the CND method.

Cadmium is absorbed by plants rapidly and without control through the same channels as other essential metals, interfering with their transport and utilization. Many studies have shown that selenium could be utilized as a way to avoid this unwanted transport and other negative effects of Cd. For this reason, the present research study was conducted with four treatments (−Cd/−Se, +Cd/−Se, +Cd/+Se_F, and +Cd/+Se_R) to determine the type of application of Se that is best (foliarly and/or via the root) as regards the reduction of the toxic effects of Cd on plants. Our results showed that the Cd excess in the nutrient solution resulted in a decrease in the total dry biomass of the plants grown under these conditions, and this decrease was due to the reduction of the growth of the shoot (48% +Cd/−Se, 45% +Cd/+Se_F, and 38% +Cd/+Se_R, relative to −Cd/−Se). This reduction in growth was due to: (i) the toxicity of Cd itself and (ii) the nutritional disequilibrium suffered by the plants. It seems that under hydroponic conditions, the addition of Se to the nutrient solution, and therefore its absorption through the roots (lower antioxidant activity, superoxide dismutase, H₂O₂ concentration and higher catalase activity), greatly delayed and reduced the toxic effects of Cd on the pepper plants, as opposed to the foliar application of this element.

Previous studies have shown that the application of selenium (Se) can efficiently mitigate the toxic effects of cadmium (Cd) on various crops. The objective of the present work is to decipher the mechanisms responsible for the efficiency of Se against the effects of Cd in pepper plants, with respect to the carbon and nitrogen metabolism. The following were analyzed: the concentrations of anions related with this metabolism, such as nitrates, nitrites, and ammonium, the activities of different enzymes such as nitrate reductase, nitrite reductase, and glutamate synthase, polyamines in their different forms, organic acid salts, amino acids, and sugars in the leaf and root tissues of the pepper plants grown in a hydroponics system. Four different treatments were applied: plants without Cd or Se applied (−Cd/−Se); plants grown with Cd added to the nutrient solution (NS) but without Se (+Cd/−Se); plants grown with Cd in the NS, and with the foliar application of Se (+CD/+SeF); and lastly, plants grown with Cd in the NS, and with Se applied to the root (+Cd/+SeR). The metabolites and enzymes related with carbon and nitrogen metabolism were analyzed 15 days after the application. The results showed the superiority of the +Cd/+SeR treatment with respect to the +Cd/+SeF treatment, as shown by an increase in the conjugated polyamines, the decrease in glutamate and phenylalanine, and the increase of malate and chlorogenic acid. The results indicated that SeR decreased the accumulation and toxicity of Se as polyamine homeostasis improved, defense mechanisms such as the phenylpropanoid increased, and the entry of Cd into the plants was blocked.