An Staes

Dr. An Staes

orcid

Research Focus & Metrics

Academic Career Length

25Years (2002-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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31 March 2003 in Nature Biotechnology
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Journal Article
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KGKris Gevaert
Kris Gevaert
MGMarc Goethals
Marc Goethals
LMLennart Martens
Lennart Martens
JDJozef Van Damme
Jozef Van Damme
An StaesAn Staes
An Staes
JVJoël Vandekerckhove
Joël Vandekerckhove

22 March 2019 in Science
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Journal Article
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THTim Hander
Tim Hander
ÁFÁlvaro D. Fernández-Fernández
Álvaro D. Fernández-Fernández
RKRobert P. Kumpf
Robert P. Kumpf
PWPatrick Willems
Patrick Willems
HSHendrik Schatowitz
Hendrik Schatowitz
SSSimon Stael
Simon Stael
Rapid response to tissue damage: Damaged plants are susceptible to microbial attack. In response to physical damage, plants proactively generate signal peptides to activate their immune systems. Hander et al. examined wound responses in the model plant Arabidopsis thaliana . They identified a metacaspase that releases an immunomodulatory signal peptide from its precursor form within 30 seconds of the damage. The metacaspase itself was activated by a burst of calcium released by tissue damage. Science , this issue p. eaar7486

1 December 2018 in Cell Metabolism
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Journal Article
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UBUlrike Bruning
Ulrike Bruning
FMFrancisco Morales-Rodriguez
Francisco Morales-Rodriguez
JKJoanna Kalucka
Joanna Kalucka
JGJermaine Goveia
Jermaine Goveia
FTFederico Taverna
Federico Taverna
PCPeter Carmeliet
Peter Carmeliet

1 November 2002 in Molecular & Cellular Proteomics
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Journal Article
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KGKris Gevaert
Kris Gevaert
JDJozef Van Damme
Jozef Van Damme
MGMarc Goethals
Marc Goethals
GTGrégoire R. Thomas
Grégoire R. Thomas
BHBart Hoorelbeke
Bart Hoorelbeke
JVJoël Vandekerckhove
Joël Vandekerckhove
A novel gel-free proteomic technology was used to identify more than 800 proteins from 50 million Escherichia coli K12 cells in a single analysis. A peptide mixture is first obtained from a total unfractionated cell lysate, and only the methionine-containing peptides are isolated and identified by mass spectrometry and database searching. The sorting procedure is based on the concept of diagonal chromatography but adapted for highly complex mixtures. Statistical analysis predicts that we have identified more than 40% of the expressed proteome, including soluble and membrane-bound proteins. Next to highly abundant proteins, we also detected low copy number components such as the E. coli lactose operon repressor, illustrating the high dynamic range. The method is about 100 times more sensitive than two-dimensional gel-based methods and is fully automated. The strongest point, however, is the flexibility in the peptide sorting chemistry, which may target the technique toward quantitative proteomics of virtually every class of peptides containing modifiable amino acids, such as phosphopeptides, amino-terminal peptides, etc., adding a new dimension to future proteome research.

22 September 2005 in Nature Methods
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Journal Article
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PDPetra Van Damme
Petra Van Damme
LMLennart Martens
Lennart Martens
JDJozef Van Damme
Jozef Van Damme
KHKoen Hugelier
Koen Hugelier
An StaesAn Staes
An Staes
KGKris Gevaert
Kris Gevaert

14 July 2011 in Nature Protocols
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Journal Article
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An StaesAn Staes
An Staes
FIFrancis Impens
Francis Impens
PDPetra Van Damme
Petra Van Damme
BRBart Ruttens
Bart Ruttens
MGMarc Goethals
Marc Goethals
KGKris Gevaert
Kris Gevaert