Anaerobic digestion (AD) is an important step in waste recovery. In Colombia, the production of citrus food significantly contributes to environmental impact via waste generation. In 2021, the waste produced, specifically citrus rind, amounted to 725,035 tons/year. During degradation, wastes generate leachate and greenhouse gases (GHGs), which negatively impact water sources (leachate), soil, and human and animal health. This article describes the design of a two-phase biodigestion system for the degradation of organic matter and biogas production. The system uses citrus waste to produce biogas with neutral emissions. The biodigestion process begins with the stabilization of the methanogenesis reactor (UASB), which takes approximately 19 days. During this period, the biogas produced contains approximately 60% methane by volume. Subsequently, the packed bed reactor operates for 7 days, where hydrolytic and acetogenic bacteria decompose the citrus waste, leading to the production and accumulation of volatile fatty acids. The final step involves combining the two phases for 5 days, resulting in a daily biogas production ranging from 700 to 1100 mL. Of this biogas, 54.90% is methane ($\mathrm{C}{\mathrm{H}}_4$) with a yield of $0.51 \mathrm{L}\mathrm{C}{\mathrm{H}}_4{\left(\mathrm{g}\mathrm{S}\mathrm{V}\right)}^{-1}$. This study assesses the methane production capacity of citrus waste, with the process benefiting from the pH value of the leachate, enhancing its degradability. Consequently, this approach leads to a notable 27.30% reduction in solids within the digestion system. The two-phase anaerobic biodigestion system described in this article demonstrates a promising method to mitigate the environmental impact of citrus waste while concurrently producing a renewable source of energy.

La bioeconomía es una misión de largo alcance que se consolida como una apuesta de país en la que el conocimiento y el capital natural convergen y se trasforman en una potente fuerza de crecimiento económico y desarrollo social y sostenible. En concordancia con lo anterior, la Universidad de Ibagué tiene como objetivo principal generar acciones académicas, de investigación y proyección social, de alto valor, que creen y potencien capacidades para la región. En este sentido, ha confiado en este modelo como motor de desarrollo económico para el departamento y el país, empleando procesos de investigación, desarrollo e innovación (I+D+i).