Eleni Bozinou

Dr. Eleni Bozinou

Food Science and Nutrition, University of Thessaly, Vólos, Thessalía, Greece
orcid
PostDoc Researcher

Research Focus & Metrics

Academic Career Length

8Years (2019-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

View All Publications
6 November 2023 in Biomass
Verified
Journal Article
Open access
PDF file
Theodoros ChatzimitakosTheodoros Chatzimitakos
Theodoros Chatzimitakos
Vassilis AthanasiadisVassilis Athanasiadis
Vassilis Athanasiadis
Dimitrios Rafail KalompatsiosDimitrios Rafail Kalompatsios
Dimitrios Rafail Kalompatsios
Martha Maria MantiniotouMartha Maria Mantiniotou
Martha Maria Mantiniotou
Eleni BozinouEleni Bozinou
Eleni Bozinou
Stavros I. LalasStavros I. Lalas
Stavros I. Lalas
The food processing industry is a continuously developing sector that uses innovative technologies to efficiently process food products. During processing, food industries generate substantial amounts of by-products in the form of waste materials. This food waste consists of organic matter rich in bioactive compounds, such as polyphenols, carotenoids, and flavonoids. Improper management of food waste can adversely affect both the environment and human health, leading to environmental pollution and the release of greenhouse gas emissions. Thus, proper food waste management has become an urgent global issue. The presence of bioactive compounds (mainly polyphenols, flavonoids, and anthocyanins, but also carotenoids, alkaloids, proteins, lipids, and carbohydrates) in food waste holds the potential to transform them into valuable resources. Several sectors, including food and energy, have recognized food waste as an innovative source. Recently, much emphasis has been placed on optimizing the extraction yield of such bioactive compounds through the utilization of environmentally friendly and sustainable methodologies and solvents. Pulsed electric field (PEF)-assisted extraction is an emerging technique that holds promise for the utilization of waste materials. PEF technology can efficiently optimize the extraction of valuable compounds within a shorter time while minimizing solvent and energy consumption. In this review, we provide a comprehensive overview of the current state of PEF technology and its implications for recovering bioactive compounds from food waste. The integration of innovative technologies like PEF in the food processing industry can play a crucial role in managing food waste sustainably, reducing environmental impact, and harnessing the full potential of bioactive compounds contained in these waste materials. The objective of this critical review is to provide an overview of the utilization of PEF pretreatment for food by-products and to conduct a comparative analysis with other extraction techniques.

23 June 2023 in Oxygen
Verified
Journal Article
Open access
PDF file
DPDimitrios Palaiogiannis
Dimitrios Palaiogiannis
Theodoros ChatzimitakosTheodoros Chatzimitakos
Theodoros Chatzimitakos
Vassilis AthanasiadisVassilis Athanasiadis
Vassilis Athanasiadis
Eleni BozinouEleni Bozinou
Eleni Bozinou
Dimitris P. MakrisDimitris P. Makris
Dimitris P. Makris
Stavros I. LalasStavros I. Lalas
Stavros I. Lalas
The aim of this study was to evaluate the efficiency of successive extraction (using solvents of increasing polarity, namely hexane, ethyl acetate, acetone, ethanol, and water) of polyphenols from Cistus creticus L. The results were compared with the ones obtained from a single-solvent extraction (ethanol, water, and 50% ethanol: water). According to the results, each solvent used for extraction had a significant effect on the yield of extracted polyphenols and the antioxidant activity of the extracts. The highest extraction yield for successive extraction was achieved with ethanol (95.33 mg GAE/g), whereas a comparable amount could also be extracted with 50% ethanol: water mixture single-solvent extraction (96.51 mg GAE/g). The ethanolic and aqueous extracts had the highest antioxidant activity as indicated by their lowest IC50 values in the DPPH assay, specifically 350.99 μg/mL for ethanolic extract of successive extraction and 341.18 μg/mL for 50% ethanol: water mixture of single-solvent extraction, followed by the extract produced using acetone. However, the acetone extract contained more flavonoids than the other two extracts up to 28.03 mg QE/g. The results obtained were in line with those for the single-solvent extraction. It is concluded that using a range of solvents in succession is a more efficient way of extracting higher amounts of antioxidant compounds with varying antioxidant activity.

25 November 2019 in Antioxidants
Verified
Journal Article
Open access
PDF file
ALACHILLIA LAKKA
ACHILLIA LAKKA
Spiros GrigorakisSpiros Grigorakis
Spiros Grigorakis
IKIoanna Karageorgou
Ioanna Karageorgou
GBGeorgia Batra
Georgia Batra
OKOlga Kaltsa
Olga Kaltsa
Dimitris P. MakrisDimitris P. Makris
Dimitris P. Makris
The current investigation was undertaken to examine saffron processing waste (SPW) as a bioresource, which could be valorized to produce extracts rich in antioxidant polyphenols, using a green, natural deep eutectic solvent (DES). Initially, there was an appraisal of the molar ratio of hydrogen bond donor/hydrogen bond acceptor in order to come up with the most efficient DES composed of L-lactic acid/glycine (5:1). The following step was the optimization of the extraction process using response surface methodology. The optimal conditions thus determined were a DES concentration of 55% (w/v), a liquid-to-solid ratio of 60 mL g−1, and a stirring speed of 800 rounds per minute. Under these conditions, the extraction yield in total polyphenols achieved was 132.43 ± 10.63 mg gallic acid equivalents per g of dry mass. The temperature assay performed within a range of 23 to 80 °C, suggested that extracts displayed maximum yield and antioxidant activity at 50–60 °C. Liquid chromatography-mass spectrometry analysis of the SPW extract obtained under optimal conditions showed that the predominant flavonol was kaempferol 3-O-sophoroside and the major anthocyanin delphinidin 3,5-di-O-glucoside. The results indicated that SPW extraction with the DES used is a green and efficient methodology and may afford extracts rich flavonols and anthocyanins, which are considered to be powerful antioxidants.

13 January 2019 in Beverages
Verified
Journal Article
Open access
PDF file
Eleni BozinouEleni Bozinou
Eleni Bozinou
IKIoanna Karageorgou
Ioanna Karageorgou
GBGeorgia Batra
Georgia Batra
VDVassilis Dourtoglou
Vassilis Dourtoglou
Stavros I. LalasStavros I. Lalas
Stavros I. Lalas
The scope of this work was to determine the possibility of the application of the pulsed electric field (PEF) technique to the production of extracts from Moringa oleifera plant material (freeze-dried leaves). Various PEF conditions (pulse duration—PD; and pulse interval—PI) were tested. A field strength of 7 kV/cm was used. The total phenols in the extracts were evaluated by the Folin–Ciocalteu method and the antioxidant activity was evaluated by the radical scavenging activity (DPPH•), ferric reducing antioxidant power (FRAP) and Rancimat methods. The results were compared with those of the extracts obtained using other extraction techniques, namely microwave-assisted and ultrasound-assisted extractions, simple boiling water extraction, and plain maceration with water (as the control). The highest extraction of total phenols was achieved by the PEF procedure using 40 min treatment at a PD of 20 msec and a PI of 100 μsec. Additionally, all methods for the determination of the antioxidant activity showed that the activity of the extracts was proportional to the total phenol content. Concerning the PEF procedure, a low pulse duration with a high pulse interval is proposed in order to achieve higher extraction efficiency.

4 October 2025 in Foods
Verified
Journal Article
Open access
PDF file
KLKonstantinos George Liakos
Konstantinos George Liakos
Vassilis AthanasiadisVassilis Athanasiadis
Vassilis Athanasiadis
Eleni BozinouEleni Bozinou
Eleni Bozinou
Stavros I. LalasStavros I. Lalas
Stavros I. Lalas
The increasing complexity of modern food production demands advanced solutions for quality control (QC), safety monitoring, and process optimization. This review systematically explores recent advancements in machine learning (ML) for QC across six domains: Food Quality Applications; Defect Detection and Visual Inspection Systems; Ingredient Optimization and Nutritional Assessment; Packaging—Sensors and Predictive QC; Supply Chain—Traceability and Transparency and Food Industry Efficiency; and Industry 4.0 Models. Following a PRISMA-based methodology, a structured search of the Scopus database using thematic Boolean keywords identified 124 peer-reviewed publications (2005–2025), from which 25 studies were selected based on predefined inclusion and exclusion criteria, methodological rigor, and innovation. Neural networks dominated the reviewed approaches, with ensemble learning as a secondary method, and supervised learning prevailing across tasks. Emerging trends include hyperspectral imaging, sensor fusion, explainable AI, and blockchain-enabled traceability. Limitations in current research include domain coverage biases, data scarcity, and underexplored unsupervised and hybrid methods. Real-world implementation challenges involve integration with legacy systems, regulatory compliance, scalability, and cost–benefit trade-offs. The novelty of this review lies in combining a transparent PRISMA approach, a six-domain thematic framework, and Industry 4.0/5.0 integration, providing cross-domain insights and a roadmap for robust, transparent, and adaptive QC systems in the food industry.

Verified
Journal Article
Open access
PDF file
Vassilis AthanasiadisVassilis Athanasiadis
Vassilis Athanasiadis
Theodoros ChatzimitakosTheodoros Chatzimitakos
Theodoros Chatzimitakos
KKKonstantina G. Kotsou
Konstantina G. Kotsou
Dimitrios Rafail KalompatsiosDimitrios Rafail Kalompatsios
Dimitrios Rafail Kalompatsios
Eleni BozinouEleni Bozinou
Eleni Bozinou
Stavros I. LalasStavros I. Lalas
Stavros I. Lalas
Nowadays, more and more researchers engage in studies regarding the extraction of bioactive compounds from natural sources. To this end, plenty of studies have been published on this topic, with the interest in the field growing exponentially. One major aim of such studies is to maximize the extraction yield and, simultaneously, to use procedures that adhere to the principles of green chemistry, as much as possible. It was not until recently that pulsed electric field (PEF) technology has been put to good use to achieve this goal. This new technique exhibits many advantages, compared to other techniques, and they have successfully been reaped for the production of extracts with enhanced concentrations in bioactive compounds. In this advancing field of research, a good understanding of the existing literature is mandatory to develop more advanced concepts in the future. The aim of this review is to provide a thorough discussion of the most important applications of PEF for the enhancement of polyphenols extraction from fresh food products and by-products, as well as to discuss the current limitations and the prospects of the field.