The global shortage of corneal transplants has spurred an urgency in the quest for efficient treatments. This systematic review not only provides a concise overview of the current landscape of corneal morphology, physiology, diseases, and conventional treatments but crucially delves into the forefront of tissue engineering for corneal regeneration. Emphasizing cellular and acellular components, bioprinting techniques, and pertinent biological assays, it explores optimization strategies for manufacturing and cost-effectiveness. To bridge the gap between research and industrial production, the review outlines the essential regulatory strategy required in Europe, encompassing relevant directives, frameworks, and governing bodies. This comprehensive regulatory framework spans the entire process, from procuring initial components to marketing and subsequent product surveillance. In a pivotal shift towards the future, the review culminates by highlighting the latest advancements in this sector, particularly the integration of tissue therapy with artificial intelligence. This synergy promises substantial optimization of the overall process, paving the way for unprecedented breakthroughs in corneal regeneration. In essence, this review not only elucidates the current state of corneal treatments and tissue engineering but also outlines regulatory pathways and anticipates the transformative impact of artificial intelligence, providing a comprehensive guide for researchers, practitioners, and policymakers in the field.

Traditional 3D bioprinting of corneal constructs relies on planar slicing, which often results in a significant stairstep effect and the loss of anatomical curvature. Curvilinear layering has emerged as a promising alternative to address these limitations. The presented methodology, based on non-planar layer integration, ensures a smoother surface finish. The model’s surface is identified via vertex normals and reconstructed using the Poisson method. Finally, surface parametrization is applied to generate spatially curved trajectories. To validate the algorithm, corneal constructs were printed using a planar and the proposed non-planar approach. Quantitative evaluation of micro-Computed Tomography data revealed that the non-planar approach achieved significantly higher morphological fidelity, successfully replicating the intended parabolic profile of the human cornea. Furthermore, the non-planar constructs demonstrated adequate functional performance, characterized by high optical transparency. Thereby, the feasibility of printing non-planar layers using the proposed novel approach is successfully demonstrated. Furthermore, the comparative analysis confirms the method’s potential for corneal biofabrication when compared to traditional planar methods.