Persistent toxic substances including persistent organic pollutants and heavy metals have been released in high quantities in surface waters by industrial activities. Their presence in environmental compartments is causing harmful effects both on the environment and human health. It was shown that their removal from wastewaters using conventional methods and adsorbents is not always a sustainable process. In this circumstance, the use of microorganisms for pollutants uptake can be seen as being an environmentally-friendly and cost-effective strategy for the treatment of industrial effluents. However, in spite of their confirmed potential in the remediation of persistent pollutants, microorganisms are not yet applied at industrial scale. Thus, the current paper aims to synthesize and analyze the available data from literature to support the upscaling of microbial-based biosorption and bioaccumulation processes. The industrial sources of persistent pollutants, the microbial mechanisms for pollutant uptake and the significant results revealed so far in the scientific literature are identified and covered in this review. Moreover, the influence of different parameters affecting the performance of the discussed systems and also very important in designing of treatment processes are highly considered. The analysis performed in the paper offers an important perspective in making decisions for scaling-up and efficient operation, from the life cycle assessment point of view of wastewater microbial bioremediation. This is significant since the sustainability of the microbial-based remediation processes through standardized methodologies such as life cycle analysis (LCA), hasn’t been analyzed yet in the scientific literature.

Accelerating heavy metal pollution is a hot issue due to a continuous growth in consumerism and increased activities in various global industries. Soil contamination with heavy metals has resulted in their incorporation into the human food web via plant components. Accumulation and amplification of heavy metals in human tissues through the consumption of medicinal plants can have hazardous health outcomes. Therefore, in this critical review we aim to bring together published information on this subject, with a special highlight on the knowledge gaps related to heavy metal stress in medicinal plants, their responses, and human health related risks. In this respect, this review outlines the key contamination sources of heavy metals in plants, as well as the absorption, mobilization and translocation of metal ions in plant compartments, while considering their respective mechanisms of detoxification. In addition, this literature review attempts to highlight how stress and defensive strategies operate in plants, pointing out the main stressors, either biotic or abiotic (e.g., heavy metals), and the role of reactive oxygen species (ROS) in stress answers. Finally, in our research, we further aim to capture the risks caused by heavy metals in medicinal plants to human health through the assessment of both a hazard quotient (HQ) and hazard index (HI).

Given the current highest demand in history for raw materials, there is a growing demand for the recovery of key metals from secondary sources, in order to prevent metal depletion and to reduce the risk of toxic discharges into the environment. This paper focuses on the current nature-based solutions (i.e., biomining and bioleaching) applied to resource recovery (metals) from solid matrices. Biomining exploits the potential of microorganisms to facilitate the extraction and recovery of metals from a wide range of waste materials as an interesting alternative, replacing primary raw materials with secondary material resources (thus improving metal recycling rates in the context of the circular economy). Special attention was paid to the analysis of metal biomining from a process sustainability perspective. In this regard, several supporting tools (e.g., life cycle assessment, LCA), developed to assist decision-makers in the complex process of assessing and scaling-up remediation projects (including biomining), were discussed. The application of LCA in biomining is still evolving, and requires comprehensive case studies to improve the methodological approach. This review outlines the fact that few studies have focused on demonstrating the environmental performance of the biomining process. Also, further studies should be performed to promote the commercial opportunities of biomining, which can be used to recover and recycle metals from solid matrices and for site remediation. Despite some important disadvantages (poor process kinetics; metal toxicity), biomining is considered to be a cleaner approach than conventional mining processes. However, implementing it on a large scale requires improvements in regulatory issues and public acceptance.

Wild Iris plants are usually found in spontaneous flora, but due to their ornamental characteristics, they can also be used for ornamental purposes, which means that it is very important to find the perfect conditions for plant growth. This research aimed to evaluate the ornamental value and adaptive behavior of wild Iris aphylla L. in “ex situ” conditions. Plants from wild flora were cultivated experimentally in the Floriculture field at the Faculty of Horticulture, IULS, Iași, Romania. The biometric determinations revealed the significantly higher ornamental value of conserved plants grown in “ex situ” conditions compared to wild plants. In “ex situ” conditions, the plants displayed more vigorous growth (~100%) and had a higher number of flowers per stem (5–9 flowers), whereas, in wild conditions, this species has from two to a maximum of five flowers. Given the absence of anatomical studies in the literature, detailed anatomical investigations of the leaf structure were performed, complemented by analyses of the photosynthetic pigment content to assess the plant’s physiological performance. Additionally, the molecular phylogenetic analyses conducted using two plastid markers (rbcL and trnL-F) confirmed the taxonomic classification of the native I. aphylla L. species. To the best of our knowledge, this is the first report on the molecular phylogeny of the wild Iridaceae species in Romania. These findings provide insights into the taxonomy, morphology, cultivation potential, and ornamental value of the species, supporting future conservation and horticulture development programs.

Heavy metal pollution is a serious concern of the modern era due to its widespread negative effects on human health and to the environment. Conventional technologies applied for the uptake of this category of persistent pollutants are complex, often expensive, and inefficient at low metal concentrations. In the last few years, non-conventional alternatives have been studied in search of better solutions in terms of costs and sustainability. Microbial adsorbents are one of the biomass-based sorbents that have extensively demonstrated excellent heavy metals removal capacity even at low concentrations. However, most of the carried-out research regarding their application in wastewater treatment has been performed in discontinuous systems. The use of microorganisms for the uptake of metal ions in continuous systems could be an important step for the upscale of the remediation processes since it facilitates a faster remediation of higher quantities of wastewaters loaded with heavy metals, in comparison with batch systems removal. Thus, the current research aims to analyze the available studies focusing on the removal of metal ions from wastewaters using microorganisms, in continuous systems, with a focus on obtained performances, optimized experimental conditions, and the sustainability of the bioremoval process. The present work found that microbial-based remediation processes have demonstrated very good performances in continuous systems. Further sustainability analyses are required in order to apply the bioremediation technology in an optimized environmentally friendly way in large-scale facilities.

The increasing consumption of electrical and electronic equipment (EEE), correlated with the fast innovation pace in this field, generates a large amount of annual waste. The current established management practices cannot keep up with it, and the results are of increased significance given the negative effects on the environment and human health. Thus, the current study aimed to analyze the environmental impact of three different scenarios of waste electrical and electronic equipment (WEEE) management, following population awareness campaigns regarding its collection in the Municipality of Iasi, Romania. Data processing was carried out considering Life Cycle Assessment (LCA) methodology with the established functional unit for each scenario according to the collected amount. The results were quantified using the CML2001 and ReCiPe methods and showed that the highest environmental impact was obtained for scenario II (S2) (1.59 × 10⁻⁷ pers. equiv. using the CML2001 method and 32.7 pers. equiv. using the ReCiPe method), while the lowest for scenario I (S1) (6.42 × 10⁻⁸ pers. equiv. using the CML2001 method and 13.8 pers. equiv. using the ReCiPe method). The process with the highest contribution to the total environmental impact was the collection stage for all scenarios, with the exception of scenario S2, in which case the highest value was generated for the landfill process following the application of the ReCiPe method (39.93%). The current study provides value to a critical issue in the environmental area and supports the development of sustainable WEEE management processes.