Background: Gout is a chronic inflammatory condition characterized by elevated uric acid levels in the blood, which can precipitate acute gout attacks in individuals with genetic susceptibility, existing medical conditions, and dietary influences. Genetic predispositions, comorbid medical conditions, nutritional choices, and environmental factors increasingly recognize the multifactorial etiology of the disease. Methods: Recent research has highlighted the potential of phytochemicals, particularly flavonoids, saponins, and alkaloids, to manage hyperuricemia (HUA) and its associated complications. Results: Plant’s natural compounds have garnered attention for their anti-inflammatory, antioxidant, and uric acid-lowering properties, suggesting their role in alternative and complementary medicine. Phytochemicals have demonstrated promise in mitigating gout symptoms and potentially modifying the disease course by addressing different aspects of hyperuricemia and inflammation. Herbal remedies, with their complex phytochemical profiles, offer a unique advantage by potentially complementing conventional pharmacological treatments. The integration of herbal therapies with standard medications could lead to enhanced therapeutic outcomes through synergistic effects, optimizing disease management, and improving patient quality of life. Conclusions: This review examines the current understanding of the multifaceted etiology of gout, explores the role of phytochemicals in managing hyperuricemia, and discusses the potential benefits of combining herbal remedies with conventional treatments to improve patient care and therapeutic efficacy.

MTS1338, a distinctive small RNA in pathogenic mycobacteria, plays a crucial role in host–pathogen interactions during infection. Mycobacterial cells encounter heterogeneous stresses in macrophages, which highly upregulate MTS1338. A dormancy regulatory factor DosR regulates the intracellular abundance of MTS1338. Herein, we investigated the interplay of DosR and a low pH‐inducible gene regulator PhoP binding to the MTS1338 promoter. We identified that DosR strongly binds to two regions upstream of the MTS1338 gene. The proximal region possesses a threefold higher affinity than the distal site, but the presence of both regions increased the affinity for DosR by > 10‐fold. PhoP did not bind to the MTS1338 gene but binds to the DosR‐bound MTS1338 gene, suggesting a concerted mechanism for MTS1338 expression.

Multidrug resistance (MDR) is an emerging global health concern worldwide, driving the need for innovative solutions. Herbal approaches are gaining attention and acceptance due to safer profiles and very few side effects. In this study, silver nanoparticles (VN-AgNPs) were synthesized using Vitex negundo, a medicinally valuable plant. A methanolic extract was prepared from Vitex negundo and the phytochemical evaluation confirmed the presence of flavonoids, alkaloids, and terpenoids, with quantitative analysis revealing high total phenolic content (TPC: 23.59 mg GAE/g) and total flavonoid content (TFC: 45.23 mg rutin/g), both maximized under microwave-assisted extraction (MAE). The antioxidant activity was also highest (18.77 mg AA/g). Characterization of the optimized extract by GC–MS identified various bioactive compounds. VN-AgNPs were synthesized using the aqueous leaf extract under specified conditions and were structurally characterized using many techniques and evaluated for antibacterial activity against four bacterial strains. VN-AgNPs exhibited significant antibacterial efficacy with inhibition zones measuring 16 ± 0.87 mm against Bacillus (Gram-positive), 15 ± 0.46 mm against E. coli (Gram-negative), 12 ± 0.64 mm against Pseudomonas (Gram-negative), and 11 ± 0.50 mm against Pectobacterium (Gram-negative plant pathogen). These findings highlight the efficacy of green-synthesized VN-AgNPs as a promising alternative to combat MDR pathogens, paving the way for sustainable and effective antimicrobial strategies.