Biological, physicochemical, structural, and thermal properties of PVA-based electrospun wound dressings added with hydrolyzed collagen (HC) and different concentrations of Hypericum perforatum ethanolic extract (EEHP) were studied. Membrane characterization was carried out by X-ray diffraction, Fourier infrared spectroscopy, differential scanning calorimetry, barrier properties, scanning electron microscopy, image analysis (diameter and pore size), as well as antimicrobial and anti-inflammatory activities. Results showed that the PVA/HC/EEHP materials, fabricated under controlled conditions of temperature and humidity, generated fiber membranes with diameters between 140–390 nm, adequate porosity and pore size for cell growth (67–90% and 4–16 µm, respectively), and good barrier properties (0.005–0.032 g·m⁻² s⁻¹) to be used in the treatment of conditions on the skin, and was even better than some commercial products. Finally, they showed to have anti-inflammatory (>80%), and antimicrobial activity against S. aureus and S. epiderm. Furthermore, higher crystalline structure was observed according to the EEHP concentration. In addition, this is the first report in which PVA/HC/EEHP membranes are successfully fabricated and characterized.

The inadequate management of organic waste and excessive use of plastic containers cause damage to the environment; therefore, different studies have been carried out to obtain new biomaterials from agricultural subproducts. The objective of this work was to evaluate the feasibility of using the pectin extracted from the peel of Passiflora tripartita var. mollissima (PT), characterizing its type and viability for the production of edible biodegradable films. In addition, films of two thicknesses (23.45 ± 3.02 µm and 53.34 ± 2.28 µm) were prepared. The results indicated that PT is an excellent raw material for the extraction of pectin, with high yields (23.02 ± 0.02%), high galacturonic acid content (65.43 ± 2.241%), neutral sugars (ribose, xylose, glucose) and a high degree of esterification (76.93 ± 1.65%), classifying it as a high-methoxy pectin. Regarding the films, they were malleable and flexible, with a water vapor permeability from 2.57 × 10⁻¹⁰ ± 0.046 to 0.13 × 10⁻¹⁰ ± 0.029 g/s mPa according to thickness, being similar to other Passiflora varieties of edible films. The pectin extraction yield from PT makes this fruit a promising material for pectin production and its chemical composition a valuable additive for the food and pharmaceutical industries.

Valorization of by-products obtained from food processing has achieved an important environmental impact. In this research, sunflower wax recovered from oil refining process was incorporated to low and high-methoxyl pectin films produced by electrospraying. Film-forming solutions and wax-added electrosprayed films were physical and structurally evaluated. The addition of sunflower wax to the film-forming solutions reduces conductivity while raising surface tension and density, whereas the type of pectin had a larger impact on viscosity, with the low-methoxyl solution having the highest value. These changes in physical solution properties influenced the film characteristics, observing thicker films with lower water vapor transmission rate (WVTR) when adding wax. Micrographs obtained by scanning electron microscopy (SEM) revealed the presence of wax particles as small spherical shapes, having a good distribution through the sectional area of films. According to X-ray diffraction (XRD), atomic force microscopy (AFM) and mechanical properties analyses, the presence of wax had an impact on the degree of crystallinity, producing a more amorphous and rougher film’s structure, without affecting the elongation percentage and the tensile stress (p>0.05). These results showed that wax addition improves the physical properties of films, while the suitability of using both pectins and the electrospraying technique was demonstrated.

Efecto de las condiciones de atomización electrohidrodinámica sobre las características morfométricas y la resistencia mecánica de las partículas de mucílago de chía y alginato Electrohydrodynamic atomization (EHDA) is a technique which produces monodispersed particles and can employ food grade biopolymers as wall materials, such as chia mucilage. The aim of this work was to study the changes on the morphometric characteristics (Feret, roundness) and mechanical resistance of chia mucilage-sodium alginate microparticles obtained at different process conditions (voltage: 11–14 kV; distance to collector 5.5–8.5 cm). The diameter of particles was ≤1 mm, being more deformed (roundness, RD < 0.61), with higher mechanical strength (MS: 7–7.5 N) and Weber numbers (9.9 x 10^{−5 –} 3.7 × 10⁻⁴) than those processed without voltage. A descriptive model relating We and process parameters was developed, being corroborated by Pearson analysis, and observing correlations larger than 0.8 between We with Feret and MS (0.817, 0.807), and Feret with MS and voltage (0.837, 0.826). These results confirmed the feasibility of applying EHDA to produce chia mucilage-sodium alginate particles with different morphologies and mechanical characteristics depending on process conditions.

Glucose oxidase (GOX) is widely used in bakery applications to improve dough rheology and bread quality. However, its direct addition to formulations limits its functionality due to premature enzymatic activity. This study used electrospraying to encapsulate GOX using chia mucilage and sodium alginate as biopolymeric wall materials. Three drying methods—critical point drying (CPD), Lyophilization/freeze-drying (LC), and oven drying (OD)—were compared to evaluate their impact on encapsulation efficiency (EE), enzymatic activity retention, and microstructural integrity. Our findings reveal that CPD preserved the porous structure of the microcapsules, minimizing enzymatic leakage and yielding the highest EE (70%). In contrast, LC induced ice crystal formation, disrupting the polymer network and leading to a moderate EE (27.43%), whereas OD resulted in extensive capsule shrinkage, causing significant enzyme loss (57.1%). The release kinetics of GOX during mixing were best described by the Korsmeyer–Peppas model (R² = 0.999), indicating a non-Fickian diffusion mechanism influenced by polymer relaxation. These results demonstrate that drying technique selection plays a crucial role in encapsulated enzymes’ stability and release behavior, providing new insights for optimizing enzyme delivery in bakery applications.

This study aimed to develop a functional powder using whey and milk matrices, leveraging the protective capacity of chia–alginate hydrogels and the advantages of electrohydrodynamic spraying (EHDA), a non-thermal technique suitable for encapsulating probiotic cells under stress conditions commonly encountered in food processing. A hydrogel matrix composed of chia seed mucilage and sodium alginate was used to form a biopolymeric network that protected probiotic cells during processing. The encapsulation efficiency reached 99.0 ± 0.01%, and bacterial viability remained above 9.9 log₁₀ CFU/mL after lyophilization, demonstrating the excellent protective capacity of the hydrogel matrix. Microstructural analysis using confocal laser scanning microscopy (CLSM) revealed well-retained cell morphology and homogeneous distribution within the hydrogel matrix while, in contrast, scanning electron microscopy (SEM) showed spherical, porous microcapsules with distinct surface characteristics influenced by the encapsulation method. Encapsulates were incorporated into beverages flavored with red fruits and pear and subsequently freeze-dried. The resulting powders were analyzed for moisture, protein, lipids, carbohydrates, fiber, and color determinations. The results were statistically analyzed using ANOVA and response surface methodology, highlighting the impact of ingredient ratios on nutritional composition. Raman spectroscopy identified molecular features associated with casein, lactose, pectins, anthocyanins, and other functional compounds, confirming the contribution of both matrix and encapsulants maintaining the structural characteristics of the product. The presence of antioxidant bands supported the functional potential of the powder formulations. Chia–alginate hydrogels effectively encapsulated L. reuteri, maintaining cell viability and enabling their incorporation into freeze-dried beverage powders. This approach offers a promising strategy for the development of next-generation functional food gels with enhanced probiotic stability, nutritional properties, and potential application in health-promoting dairy systems.