This study aimed to evaluate essential traits of donkey’s milk and cow’s milk kefir during storage for 28 days at +4 °C. The results showed that the pH decreases significantly during fermentation from 6.75 ± 0.045 to 4.22 ± 0.062 for cow’s milk and from 7.01 ± 0.011 to 4.28 ± 0.030 for donkey’s milk. Acidity values increased significantly during storage from 63 ± 2.08 °D to 170 ± 2.80 °D for cow’s milk and from 92 ± 1.0 °D to 163 ± 1.30 °D for donkey’s milk (p < 0.05). A significant variation in total solids was observed during storage. Stability in protein content was observed for kefirs during storage time. While the level of lactose decreased significantly during storage, the fat content did not vary in kefirs during storage time at 4 °C. For microbiological properties, donkey milk kefir presents a significant difference (p < 0.05) compared to bovine kefir. Donkey’s milk always contains the lowest average germs, suggesting a better microbiological quality than cow’s milk samples. The fermented milks showed an interesting antioxidant activity measured by the DPPH and ABTS assays, which were improved during storage. The Aeromonas hydrophila was the most sensitive bacterium to the action of kefir samples. Results from the sensorial test show that participants prefer kefirs freshly prepared than those stored after 28 days at 4 °C. In conclusion, related to its unique bioactive activities and microbiological properties, donkey’s milk could be an interesting kefir fermentation source materials alternative.

This study aimed to evaluate carcass and meat quality traits in Masri (n = 14) and North African (n = 14) male donkeys, raised in a semi-intensive breeding system, grazing on mountainous areas, with supplementation of 1 kg of barley per day per animal, slaughtered at 5 (±0.48) years old. Compared to Masri, the North African population exhibited higher (p < 0.05) final body weights (181.7 ± 2.1 and 212.5 ± 7.6 kg) and cold carcass weights (101.7 ± 1.3 and 116.2 ± 4.5 kg), but lower (p < 0.05) cold dressing percentages (56.0 ± 0.4 and 54.6 ± 0.4%). Meat quality analyses showed higher (p < 0.05) cooking loss values in Masri meat (43.9 ± 0.8 vs. 39.9 ± 1.2%). Among the meat color parameters, the hue value was higher (p < 0.05) in North African samples (0.42 ± 0.01 vs. 0.39 ± 0.1). Meat from North African donkeys had higher (p < 0.05) dry matter, fat, and protein contents. Meat amino acid analysis revealed abundant levels of lysine, leucine, and methionine, in both populations. Donkey meat from both populations presented a high polyunsaturated fatty acids content, resulting in polyunsaturated fatty acids/saturated fatty acids and omega 6/omega 3 ratios for all breeds close to the recommended values for human health. Atherogenic and thrombogenic indices were also suggested to have positive effects on consumers’ artery health. The characteristics of donkey meat present intriguing nutritional aspects compared to ruminant meat, and its production should be encouraged in the rural development of inner-Mediterranean areas.

This study aimed to investigate the effect of supplementing a wheat straw-based diet with Saccharomyces cerevisiae (S.C.) on feed intake, nutrient digestibility, nitrogen balance, body weight and reproduction performance. The experiment was conducted on 14 Queue Fine de l’Ouest rams between 3 and 4 years of age (body weight (B.W.): 54.7 ± 2.03 kg; body condition score (B.C.S.): 3.5 ± 0.5), for 80 days during the breeding season. The rams were divided into two homogenous groups (n = 7), housed individually in floor pens, and allocated to two dietary treatments. The control group was offered a basal diet of 1 kg/d of wheat straw and 700 g of concentrate. The experimental group (yeast) received the same basal diet supplemented with 10 g of S.C./head/day. The results indicated that the S.C. supplementation had no significant effect on the animal’s body weight, volume and concentration of semen, dry matter intake, crude protein digestibility and nitrogen balance. Compared to the control group, the S.C. addition improved (p < 0.05) the digestibility of dry matter by 7.3%, organic matter by 11.9% and crude fiber by 24%. In addition, the mass motility score increased for the yeast group compared to the control (3.7 ± 0.24 vs. 1.9 ± 0.27, p < 0.05). The total number of dead and abnormal spermatozoa decreased for the yeast group in contrast to the control group (9.28 ± 0.95 vs. 26.8 ± 3.85% and 25.5 ± 3.33 vs. 59.2 ± 2.78%, respectively; p < 0.05). These results showed that adding S.C. to Queue Fine de l’Ouest ram’s diet during breeding season could improve nutrient digestibility and reproductive performance.

The aim of this study was to evaluate the use of horse and donkey meat in the production of kaddid—a traditional dish typically not made with these meats—from a physical and chemical perspective. The results showed that both meats exhibit similar water retention during cooking, contributing to comparable tenderness and juiciness, with no significant differences in pH values, indicating similar quality (p > 0.05). However, their amino acid profiles differ: horse meat contains lower levels of glutamate (p < 0.05), methionine (p < 0.01), isoleucine (p < 0.05), and leucine (p < 0.05), but higher levels of proline (p < 0.05), histidine (p < 0.01), and lysine (p < 0.001) compared to donkey meat. Both meats provide essential amino acids. Horse meat is richer in saturated and monounsaturated fatty acids (32.44% and 39.58%, respectively), while donkey meat has a higher content of polyunsaturated fatty acids (31.51%), with a more favorable PUFA/SFA ratio, suggesting better cardiovascular health benefits. In terms of dried meat, donkey kaddid has a higher protein (17.45 g/100 g) and lower fat content (2.1 g/100 g) compared to horse kaddid (16.7 g/100 g, and 3.5 g/100 g, respectively) (p < 0.05). These findings inform consumer choices and production practices, promoting the use of horse and donkey meat for kaddid production.

The aim of the study was to model lactation curves and assess the physicochemical properties, amino acid, and fatty acid profiles of milk from two Mediterranean donkey populations, Masri (n = 14) and North African (n = 14), using the Wood model. Over a lactation period of 205 ± 12.5 days, North African donkeys produced more milk (188.66 ± 7.19 kg) than Masri donkeys (163.42 ± 7.21 kg, p < 0.05). Peak milk yields occurred on day 57 for North African donkeys (1.212 kg/day) and day 59 for Masri donkeys (0.991 kg/day), with similar persistency indices of 7.19 and 7.21, respectively. North African donkey milk had significantly higher protein (1.45 ± 0.03 g/100 g) and β-lactoglobulin (4.75 ± 0.06 mg/mL) contents, while Masri donkey milk contained more fat (1.16 ± 0.05 g/100 g). Amino acid analysis revealed higher glutamate (0.27 ± 0.12 g/100 g) in North African donkey milk, while Masri donkey milk had more aspartate (0.16 ± 0.04 g/100 g). North African donkey milk had higher palmitic acid (20.1 ± 0.07 g/100 g), while Masri donkey milk had more oleic acid (21.4 ± 0.42 g/100 g). Lactation curve fitting yielded R² values of 93.8% for Masri donkeys and 95.7% for North African donkeys. These findings suggest that both populations are well-suited for milk production, particularly for human consumption or food applications. North African donkeys exhibited superior yields and nutrient profiles suitable for functional food applications.

Climate change, feed shortages, and rising production costs highlight the need for alternative and sustainable forages for ruminants. This study aimed to evaluate the nutritional composition, in vitro ruminal fermentation, and methane emissions of Urtica dioica ecotypes originating from contrasting bioclimatic zones in Tunisia. Aerial parts of Urtica dioica were harvested at the early flowering stage from arid, semi-arid, and sub-humid regions. Samples were subjected to chemical composition in vitro ruminal fermentation to determine dry matter degradability (DMD), neutral detergent fiber degradability (NDFD), metabolizable energy (ME), and methane production. The results demonstrate that Urtica dioica is a promising protein-rich forage, with a stable crude protein content across ecotypes (18.58–20.97% of dry matter). In contrast, NDFD, DMD, ME, and methane emissions varied significantly according to origin. The arid ecotype, characterized by the highest fiber, ether extract, and polyphenol content, exhibited the lowest DMD (53% vs. 61% and 60%), NDFD (45% vs. 55% and 56%), and ME (7.2 vs. 8.6 and 9.0 MJ/kg dry matter) but produced the lowest methane emissions (38.8 vs. 53.2 and 74.2 mL CH₄/kg DMD) compared with the semi-arid and sub-humid ecotypes. The semi-arid and sub-humid ecotypes had comparable DMD, NDFD, and ME values; however, methane emissions were higher in the sub-humid ecotype. Overall, the semi-arid ecotype provided the most favorable balance between nutritive quality and environmental sustainability. These findings highlight the critical role of ecological origin in determining the feeding value and greenhouse gas footprint of Urtica dioica, providing a scientific basis for its potential use as a sustainable forage in ruminant feeding systems.