The ability to precisely monitor the intracellular temperature directly contributes to the essential understanding of biological metabolism, intracellular signaling, thermogenesis, and respiration. The intracellular heat generation and its measurement can also assist in the prediction of the pathogenesis of chronic diseases. However, intracellular thermometry without altering the biochemical reactions and cellular membrane damage is challenging, requiring appropriately biocompatible, nontoxic, and efficient biosensors. Bright, photostable, and functionalized fluorescent nanodiamonds (FNDs) have emerged as excellent probes for intracellular thermometry and magnetometry with the spatial resolution on a nanometer scale. The temperature and magnetic field-dependent luminescence of naturally occurring defects in diamonds are key to high-sensitivity biosensing applications. Alterations in the surface chemistry of FNDs and conjugation with polymer, metallic, and magnetic nanoparticles have opened vast possibilities for drug delivery, diagnosis, nanomedicine, and magnetic hyperthermia. This study covers some recently reported research focusing on intracellular thermometry, magnetic sensing, and emerging applications of artificial intelligence (AI) in biomedical imaging. We extend the application of FNDs as biosensors toward disease diagnosis by using intracellular, stationary, and time-dependent information. Furthermore, the potential of machine learning (ML) and AI algorithms for developing biosensors can revolutionize any future outbreak.

The ability to predict the radioactive soil radon gas concentration is important for human beings because it serves as a precursor to earthquakes. Several studies have been conducted across the globe to confirm the correlation of radon emission dynamics and earthquakes, and concluded that the soil radon gas is the witness of anomalous behaviour before the occurrences of several earthquakes. This anomalous behavior can help to construct a better prediction model for earthquake forecasting. This paper aims at employing different ensemble and individual machine learning methods on real time radon time series data with different scenarios to predict anomalies in data caused by the seismic activities.The ensemble methods include boosted tree, bagged cart and boosted linear model while standalone machine learning methods include support vector machine with linear and radial kernels and k-nearest neighbors ( ${K}$ -NN). We tested the methods on a dataset recorded on the fault line located in Muzaffarabad. Time series data was collected over a period ranging from March 1, 2017 to May 11, 2018 including nine(09) earthquakes. The methods are tested in four different settings with 10 times 10 folds cross validation procedure over the time window of 1 to 4. The repeated 10 fold cross validation is performed to reduce the noise in the model performance estimation by replicating the 10 fold cross validation procedure 10 times. Statistical performance evaluation measures viz. root mean square error (RMSE), root mean squared log error (RMSLE), mean absolute percentage error (MAPE), percentage bias (PB), and mean squared error (MSE) have been calculated for the assessment of performance. In setting 1, the support vector machine with radial kernel performs better with the minimum RMSE score of 1381.023 when compared to other prediction models. In setting 3, it can be observed through different performance metrics such as RMSE, the value in the range [1262.864, 1409.616] which is minimum when other prediction models for predicting soil radon gas concentration dataset. For setting 4, the boosted tree model yielded the minimum RMSE and MAPE scores of 1573.174 and 0.056 respectively. Findings of the study shows that boosted tree and support vector machine with radial kernel proved to be better regression models for the prediction of anomalies in soil radon gas concentration during seismic activities. An important finding of this study suggests that by employing boosted tree ensemble method make us able to accurately predict soil radon gas concentration automatically from environmental parameters.

Epilepsy is a neuronal disorder for which the electrical discharge in the brain is synchronized, abnormal and excessive. To detect the epileptic seizures and to analyse brain activities during different mental states, various methods in non-linear dynamics have been proposed. This study is an attempt to quantify the complexity of control and epileptic subject with and without seizure as well as to distinguish eye-open (EO) and eye-closed (EC) conditions using threshold-based symbolic entropy. The threshold-dependent symbolic entropy was applied to distinguish the healthy and epileptic subjects with seizure and seizure-free intervals (i.e. interictal and ictal) as well as to distinguish EO and EC conditions. The original time series data was converted into symbol sequences using quantization level, and word series of symbol sequences was generated using a word length of three or more. Then, normalized corrected Shannon entropy (NCSE) was computed to quantify the complexity. The NCSE values were not following the normal distribution, and the non-parametric Mann–Whitney–Wilcoxon (MWW) test was used to find significant differences among various groups at 0.05 significance level. The values of NCSE were presented in a form of topographic maps to show significant brain regions during EC and EO conditions. The results of the study were compared to those of the multiscale entropy (MSE). The results indicated that the dynamics of healthy subjects are more complex compared to epileptic subjects (during seizure and seizure-free intervals) in both EO and EC conditions. The comparison of the dynamics of epileptic subjects revealed that seizure-free intervals are more complex than seizure intervals. The dynamics of healthy subjects during EO conditions are more complex compared to those during EC conditions. Further, the results clearly demonstrated that threshold-dependent symbolic entropy outperform MSE in distinguishing different physiological and pathological conditions. The threshold symbolic entropy has provided improved accuracy in quantifying the dynamics of healthy and epileptic subjects during EC an EO conditions for each electrode compared to the MSE.

This Breast Cancer in women is the most frequency diagnosed and second leading cause of cancer deaths. Due to complex nature of microcalcification and masses, radiologist fail to properly diagnose breast cancer. In past researchers developed Computer aided diagnosis (CAD) systems that help the radiologist to detect abnormalities in an efficient manner. In this research, we have employed robust Machine learning classification techniques such as Support vector machine (SVM) kernels and Decision Tree to distinguish cancer mammograms from normal subjects. Different features are proposed such as texture, morphological entropy based, scale invariant feature transform (SIFT), and elliptic Fourier descriptors (EFDs). These features are passed as input to ML classifiers. Jack-knife 10-fold cross validation was used and performance evaluated in term of specificity, sensitivity, Positive predive value (PPV), negative predictive value (NPV), false positive rate (FPR) and receive operating curve (ROC). The highest performance based on single feature extracting strategy was obtained using Bayesian approach with texture and EFDs features, and SVM RBF and Gaussian kernels with EFDs features whereas highest AUC with single feature was obtained using Bayesian approach by extracting texture, morphological, EFDs and entropy features and SVM RBF and Gaussian kernels with EFDs features.