The present study aimed to evaluate the feasibility of developing low-cost N- and Fe-doped TiO₂ photocatalysts for investigating the mineralization of 2,4-dimethylaniline (2,4-DMA). With a single anatase phase, the photocatalysts showed high thermal stability with mass losses of less than 2%. The predominant oxidative state is Ti⁴⁺, but there is presence of Ti³⁺ associated with oxygen vacancies. In materials with N, doping was interstitial in the NH₃/NH⁴⁺ form and for doping with Fe, there was a presence of Fe-Ti bonds (indicating substitutional occupations). With an improved band gap energy from 3.16 eV to 2.82 eV the photoactivity of the photocatalysts was validated with an 18 W UVA lamp (340–415 nm) with a flux of 8.23 × 10⁻⁶ Einstein s⁻¹. With a size of only 14.45 nm and a surface area of 84.73 m² g⁻¹, the photocatalyst doped with 0.0125% Fe mineralized 92% of the 2,4-DMA in just 180 min. While the 3% N photocatalyst with 12.27 nm had similar performance at only 360 min. Factors such as high surface area, mesoporous structure and improved E_bg, and absence of Fe peak in XPS analysis indicate that doping with 0.0125% Fe caused a modification in TiO₂ structure.

In order to know the behavior and performance of a vertical flow constructed wetland (VF-CW) operating with earthworm, this study evaluated the earthworm presence linked to hydraulic loading rates applied for domestic wastewater treatment. Two VF-CW units (710 cm², 0.75 m deep, with sand as filter media and planted with Heliconia rostrata) operated with a variable hydraulic and organic loadings rates (200 mm d^-1 / 123 g COD m^-2 d^-1; 280 mm d^-1 / 186 g COD m^-2 d^-1; 160 mm d^-1 / 94 g COD m^-2 d^-1). Although the efficiency of COD load removal was similar (around 70%) for both CWs, the efficiency of total nitrogen (TN) load removal was around 95% throughout the evaluated period. The nitrification-denitrification process was identified with and without presence of earthworm. VF-CW with earthworms (VF-WE) showed higher hydraulic conductivity values (from 0.11 to 0.14 m h^-1) compared with the VF-CW wetland without earthworms (VF-NE) (0.07 to 0.09 m h^-1). This study showed that the use of earthworms in CWs can be associated mainly with a preventive measure of clogging, which requires measures to maintain earthworms inside the bed media. Additionally, the earthworm presence has an ingestion mechanism of organic and inorganic solid particles in wastewater which excretes them as finer particles.