Md. Hakimul Haque

Dr. Md. Hakimul Haque

University of Rajshahi, Rājshāhi, Bangladesh
orcid

Research Focus & Metrics

Academic Career Length

15Years (2012-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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23 November 2020 in Biology
Verified
Journal Article
Open access
PDF file
MHMd Hakimul Haque
Md Hakimul Haque
Subir SarkerSubir Sarker
Subir Sarker
MIMd. Shariful Islam
Md. Shariful Islam
MIMd. Aminul Islam
Md. Aminul Islam
MKMd Rezaul Karim
Md Rezaul Karim
MAM. Sawkat Anwer
M. Sawkat Anwer
Antibiotic-free broiler meat production is becoming increasingly popular worldwide due to consumer perception that it is superior to conventional broiler meat. Globally, broiler farming impacts the income generation of low-income households, helping to alleviate poverty and secure food in the countryside and in semi-municipal societies. For decades, antibiotics have been utilized in the poultry industry to prevent and treat diseases and promote growth. This practice contributes to the development of drug-resistant bacteria in livestock, including poultry, and humans through the food chain, posing a global public health threat. Additionally, consumer demand for antibiotic-free broiler meat is increasing. However, there are many challenges that need to be overcome by adopting suitable strategies to produce antibiotic-free broiler meat with regards to food safety and chicken welfare issues. Herein, we focus on the importance and current scenario of antibiotic use, prospects, and challenges in the production of sustainable antibiotic-free broiler meat, emphasizing broiler farming in the context of Bangladesh. Moreover, we also discuss the need for and challenges of antibiotic alternatives and provide a future outlook for antibiotic-free broiler meat production.

5 June 2017 in Small Methods
Verified
Journal Article
Closed access
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MIMd Nazmul Islam
Md Nazmul Islam
Mostafa Kamal MasudMostafa Kamal Masud
Mostafa Kamal Masud
MHMd Hakimul Haque
Md Hakimul Haque
SHShahriar Al Hossain
Shahriar Al Hossain
YYYusuke Yamauchi
Yusuke Yamauchi
MSMuhammad J. A. Shiddiky
Muhammad J. A. Shiddiky

30 June 2017 in Chemical Communications
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Journal Article
Open access
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Mostafa Kamal MasudMostafa Kamal Masud
Mostafa Kamal Masud
MIMd Nazmul Islam
Md Nazmul Islam
MHMd Hakimul Haque
Md Hakimul Haque
STShunsuke Tanaka
Shunsuke Tanaka
VGVinod Gopalan
Vinod Gopalan
MSMuhammad J. A. Shiddiky
Muhammad J. A. Shiddiky
A nonenzymatic, amplification-free, and sensitive method for microRNA detection is reported using Au@NPFe2O3NC nanocubes.

1 June 2017 in Biosensors and Bioelectronics
Verified
Journal Article
Closed access
MIMd Nazmul Islam
Md Nazmul Islam
SYSharda Yadav
Sharda Yadav
MHMd Hakimul Haque
Md Hakimul Haque
AMAhmed Munaz
Ahmed Munaz
MIMd Farhadul Islam
Md Farhadul Islam
MSMuhammad J. A. Shiddiky
Muhammad J. A. Shiddiky

1 January 2017 in Biosensors and Bioelectronics
Verified
Journal Article
Open access
MHMd Hakimul Haque
Md Hakimul Haque
VGVinod Gopalan
Vinod Gopalan
SYSharda Yadav
Sharda Yadav
MIMd Nazmul Islam
Md Nazmul Islam
EEEhsan Eftekhari
Ehsan Eftekhari
MSMuhammad J. A. Shiddiky
Muhammad J. A. Shiddiky
We report a new method for the detection of regional DNA methylation using base-dependent affinity interaction (i.e., adsorption) of DNA with graphene. Due to the strongest adsorption affinity of guanine bases towards graphene, bisulfite-treated guanine-enriched methylated DNA leads to a larger amount of the adsorbed DNA on the graphene-modified electrodes in comparison to the adenine-enriched unmethylated DNA. The level of the methylation is quantified by monitoring the differential pulse voltammetric current as a function of the adsorbed DNA. The assay is sensitive to distinguish methylated and unmethylated DNA sequences at single CpG resolution by differentiating changes in DNA methylation as low as 5%. Furthermore, this method has been used to detect methylation levels in a collection of DNA samples taken from oesophageal cancer tissues.

1 December 2017 in Biosensors and Bioelectronics
Verified
Journal Article
Open access
MIMd Nazmul Islam
Md Nazmul Islam
VGVinod Gopalan
Vinod Gopalan
MHMd Hakimul Haque
Md Hakimul Haque
Mostafa Kamal MasudMostafa Kamal Masud
Mostafa Kamal Masud
Shahriar A. HossainShahriar A. Hossain
Shahriar A. Hossain
MSMuhammad J. A. Shiddiky
Muhammad J. A. Shiddiky
Despite having reliable and excellent diagnostic performances, the currently available messenger RNA (mRNA) detection methods mostly use enzymatic amplification steps of the target mRNA which is generally affected by the sample manipulations, amplification bias and longer assay time. This paper reports an amplification-free electrochemical approach for the sensitive and selective detection of mRNA using a screen-printed gold electrode (SPE-Au). The target mRNA is selectively isolated by magnetic separation and adsorbed directly onto an unmodified SPE-Au. The surface-attached mRNA is then measured by differential pulse voltammetry (DPV) in the presence of [Fe(CN)6]4-/3- redox system. This method circumvents the PCR amplification steps as well as simplifies the assay construction by avoiding multiple steps involved in conventional biosensing approaches of using recognition and transduction layers. Our method has demonstrated good sensitivity (LOD = 1.0pM) and reproducibility (% RSD = <5%, for n = 3) for detecting FAM134B mRNA in two cancer cell lines and a small cohort of clinical samples (number of samples = 26) collected from patients with oesophageal cancer. The analytical performance of our method is validated with a standard qRT-PCR analysis. We believe that our PCR-free approach holds a great promise for the analysis of tumor-specific mRNA in clinical samples.