Amid climate change, heatwave events are expected to increase in frequency and severity. As a result, yield losses in viticulture due to heatwave stress have increased over the years. As one of the most important crops in the world, an eco-friendly stress mitigation strategy is greatly needed. The present work aims to evaluate the physiological fitness improvement by two marine plant growth-promoting rhizobacteria consortia in Vitis vinifera cv. Antão Vaz under heatwave conditions. To assess the potential biophysical and biochemical thermal stress feedback amelioration, photochemical traits, pigment and fatty acid profiles, and osmotic and oxidative stress biomarkers were analysed. Bioaugmented grapevines exposed to heatwave stress presented a significantly enhanced photoprotection capability and higher thermo-stability, exhibiting a significantly lower dissipation energy flux than the non-inoculated plants. Additionally, one of the rhizobacterial consortia tested improved light-harvesting capabilities by increasing reaction centre availability and preserving photosynthetic efficiency. Rhizobacteria inoculation expressed an osmoprotectant promotion, revealed by the lower osmolyte concentration while maintaining leaf turgidity. Improved antioxidant mechanisms and membrane stability resulted in lowered lipid peroxidation product formation when compared to non-inoculated plants. Although the consortia were found to differ significantly in their effectiveness, these findings demonstrate that bioaugmentation induced significant heatwave stress tolerance and mitigation. This study revealed the promising usage of marine PGPR consortia to promote plant fitness and minimize heatwave impacts in grapevines.

The aquatic moss Fontinalis antipyretica requires a slow rate of dehydration to survive a desiccation event. The present work examined whether differences in the dehydration rate resulted in corresponding differences in the production of reactive oxygen species (ROS) and therefore in the amount of cell damage. Intracellular ROS production by the aquatic moss was assessed with confocal laser microscopy and the ROS-specific chemical probe 2,7-dichlorodihydrofluorescein diacetate. The production of hydrogen peroxide was also quantified and its cellular location was assessed. The rehydration of slowly dried cells was associated with lower ROS production, thereby reducing the amount of cellular damage and increasing cell survival. A high oxygen consumption burst accompanied the initial stages of rehydration, perhaps due to the burst of ROS production. A slow dehydration rate may induce cell protection mechanisms that serve to limit ROS production and reduce the oxidative burst, decreasing the number of damaged and dead cells due upon rehydration.

Green roof typology can vary depending on buildings structure, climate conditions, substrate, and plants used. In regions with hot and dry summers, such as the Mediterranean region, irrigation plays an essential role, as the highest temperatures occur during the driest period of the year. Irrigation might reduce the heat island effect and improve the cooling of buildings during this period, however, the added cost of maintenance operations and additional energy consumption could outrun the benefits provided by the project. Moreover, in situations where water is scarce or primarily channelled to other uses (e.g., domestic, agriculture or industry) during drought occurrence, it is advisable to implement green roof projects with the lowest use of water possible. The objective of the present work is to investigate solutions to optimize water use in green roofs under Mediterranean conditions, such as those of southern Europe. Two case studies are presented for Portugal, and potential techniques to reduce irrigation requirements in green roofs were tested. These addressed the use of native plant species, including the extreme type of a non-irrigated green roof (Biocrust roof) and techniques for plant installation. Plant drought tolerance was found to be an advantage in green roofs under these climatic conditions and, for the species studied, aesthetic value could be maintained when irrigation decreased.