Plant-associated endophytic fungi (EFs) are emerging as a promising solution to advancing modern agriculture and fostering environmental sustainability, especially in the face of climate change scenarios. These fungi, either naturally residing in plants or introduced through artificial inoculation techniques, improve agricultural production due to their various roles in protecting and supporting host plants. The majority of EFs serve as natural biocontrol agents for a variety of agricultural pests, such as insects, phytopathogens, nematodes, and weeds. Notably, EFs produce secondary metabolites, trigger immune responses, modify plant defense gene expression, confer host plant resistance and/or tolerance, and regulate pest growth, populations, and survival to combat agricultural pests. Beyond controlling pests, EFs promote optimal plant growth, development, and resilience by aiding in the synthesis of vital compounds such as phytohormones and bioactive metabolites, nutrient acquisition, and fortifying plants against environmental stresses and climatic changes. Moreover, the mostly nonpathogenic nature of EFs, coupled with their high yield potential, environmental safety, and cost effectiveness, positions them as eco-friendly and economically viable alternatives to synthetic agrochemicals amidst rapid climate change scenarios. As a result, the promising horizon of EFs in agricultural production necessitates interdisciplinary study and microbial modulation approaches to optimize symbiotic plant-EF relationships and their potential for improved productivity. This review provides current and comprehensive insights into the practical applications and multifaceted benefits of EFs in pest management, plant growth promotion, and climate change resilience for future agricultural production improvements. The analysis reveals the potential of developing EFs into innovative bioformulations such as biofertilizers, biostimulants, and biopesticides, thereby paving the way for their integration into a sustainable and more resilient future agricultural system.

The old-world bollworm, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae), is a destructive pest of chickpeas that has proven difficult to control using conventional methods. We evaluated the virulence of 14 isolates of Beauveria bassiana and Metarhizium anisopliae s.l. and a commercial wettable product of Bacillus thuringiensis (Bt) against larvae of H. armigera. Three isolates of B. bassiana, APPRC-9604, APPRC-T5 and DLCO-EA-56, and one of M. anisopliae strain (DLCO-EA-40) were most effective against third instar H. armigera at 10⁸ conidia/mL. At this rate, B. bassiana was more virulent to the third instar H. armigera than M. anisopliae. Among the B. bassiana isolates, APPRC-9604 had the highest virulence; i.e. LT₅₀ (4.6 days) and LC₅₀ (1.3 × 10⁴ conidia/mL). Field trials indicated that APPRC-9604 is effective in reducing larval infestations, decreasing pod damage and increasing chickpea yield. Wettable powder concentrations of Bt caused higher mortality to second instar (69%) than to third instar (56.5%) H. armigera larvae at 0.2 g/200 mL at 8 days post-treatment. Surviving larvae feeding on Bt-treated chickpea leaves required additional days for pupation and adult emergence and had a shortened adult life span compared to untreated controls. We conclude that APPRC-9604 isolate and Bt show potential as biological control agents of H. armigera larvae. Further research should focus on the effectiveness of Bt products under field conditions.

The improvement of sugarcane productivity depends on the crop varieties, growth environments, and management practices. In particular, the selection of the most productive variety and the use of an optimal fertilizer rate and plant bioregulator are critical for increasing sugarcane productivity and sugar yield. This study aimed to determine the high-performing sugarcane variety, optimal nitrogen rate, and potential bioregulator for improved sugarcane production, juice quality, and sugar yield. Two sugarcane varieties (D42/58 and NCo-334), three nitrogen (N) fertilizer rates (0, 100, and 150 kg/ha), and two bioregulators (Agrostemin and Crops®) were used for the study. The study revealed that sugarcane variety had a significant effect on all growth, yield, and quality parameters. Plant height was significantly influenced by variety and bioregulators, while stalk population was significantly influenced by two- and three-way interactions of varieties, bioregulators, and N fertilizer rates. Cane weight was significantly affected by variety, N fertilizer rate, and bioregulators, whereas cane yield was significantly affected by variety, N fertilizer, and their interaction effects. Sugar yield was similarly influenced by variety, bioregulator, and their interaction. The three main factors, as well as their two- and three-way interactions, had a considerable influence on cane quality parameters. Sugarcane variety D42/58 significantly outperformed variety NCo-334 in terms of sprouting, number of tillers, plant height, number of millable canes, and sucrose percentage by 7.49%, 9.50%, 12.80%, 10.50%, and 9.10%, respectively. The use of the D42/58 variety with N fertilizer (at 100 kg/ha) and/or the Agrostemin bioregulator also led to higher performance in cane population (107126), cane yield (153.34 tons/ha), Pol % (15.81%), and sugar yield (10.25 tons/ha). Most sugarcane growth, yield, juice quality, and sugar yield parameters were positively correlated; hence, high-performing varieties, appropriate N rates, and plant bioregulators could boost sugarcane productivity and sugar yield. Overall, the selection and combination of sugarcane variety D42/58 with 100 kg N/ha and/or Agrostemin bioregulator could maximize sugarcane production, juice quality, and sugar yield. To confirm the current findings, however, more research needs to be conducted across different agroecologies and seasons.

The fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) is among the invasive insect pests that damages maize and sorghum, the high-priority crops in newly colonized agro-ecologies, including African contexts. Owing to the increasing infestation of the pest and the limitations of current conventional methods for its management, there is a call for discovering advanced pest management approaches. RNA interference (RNAi) is an emerging molecular tool showing flexible potential for the management of S. frugiperda. We conducted a search of the recent application of RNAi literature using Google Scholar and Mendeley to find advanced papers on S. frugiperda management using RNAi molecular tools that led to growth inhibition, developmental aberrations, reduced fecundity, and mortality, mainly by disruption of normal biological processes of the pest. Although efforts have been made to accelerate the utility of RNAi, many factors limit the efficiency of RNAi to achieve successful control over S. frugiperda. Owing to RNAi’s potential bioactivity and economic and ecological acceptability, continued research efforts should focus on improving its broad applicability, including field conditions. Screening and identification of key target genes should be a priority task to achieve effective and sustainable management of this insect via RNAi. In addition, a clear understanding of the present status of RNAi utilization in S. frugiperda management is of paramount importance to improve its efficiency. Therefore, in this review, we highlight the biology of S. frugiperda and the RNAi mechanism as a foundation for the molecular management of the pest. Then, we discuss the current knowledge of the RNAi approach in S. frugiperda management and the factors affecting the efficiency of RNAi application. Finally, the prospects for RNAi-based insect pest management are highlighted for future research to achieve effective management of S. frugiperda.

The Afrotropical grasshopper genus Pteropera Karsch, 1891, is reviewed. Some species present in Cameroon are described, Pteropera augustini Donskoff, 1981, is recorded for the first time in the country, and three new species are described from Cameroon, Pteropera kennei Yetchom & Husemann, sp. nov., Pteropera matzkei Yetchom & Husemann, sp. nov. and Pteropera missoupi Yetchom & Husemann, sp. nov., increasing the number of Pteropera species in Cameroon from eight to 12, and overall to 30 species in Central Africa. An updated key of Pteropera is provided. Photographs with data on the distributions of all known species are given. In addition, a phylogenetic tree was constructed using maximum likelihood and Bayesian inference on the basis of a concatenated dataset of COI, 16S, and 12S markers of available Cameroonian species. The maximum likelihood and Bayesian inference analyses of the concatenated datasets resulted in a well-resolved phylogeny of the group and species of Pteropera were recovered as monophyletic, largely with high support. In all cases, the discrimination of all studied species based on barcode information was congruent with the species limits determined by traditional taxonomy. Our findings show the potential of integrative taxonomy to resolve the relationships among grasshoppers below the family level. Further analyses, including more comprehensive taxon sampling and additional nuclear markers, are needed, and the occurrence of several taxa still needs to be confirmed in African rainforests.