Currently, global climate change and oil pollution are two main environmental concerns for sea cucumber (Apostichopus japonicus) aquaculture. However, no study has been conducted on the combined effects of elevated temperature and oil pollution on sea cucumber. Therefore, in the present study, we treated sea cucumber with elevated temperature (26 °C) alone, water-accommodated fractions (WAF) of Oman crude oil at an optimal temperature of 16 °C, and Oman crude oil WAF at an elevated temperature of 26 °C for 24 h. Results showed that reactive oxygen species (ROS) level and total antioxidant capacity in WAF at 26 °C treatment were higher than that in WAF at 16 °C treatment, as evidenced by 6.03- and 1.31-fold-higher values, respectively. Oxidative damage assessments manifested that WAF at 26 °C treatment caused much severer oxidative damage of the biomacromolecules (including DNA, proteins, and lipids) than 26 °C or WAF at 16 °C treatments did. Moreover, compared to 26 °C or WAF at 16 °C treatments, WAF at 26 °C treatment induced a significant increase in cellular apoptosis by detecting the caspase-3 activity. Our results revealed that co-exposure to elevated temperature and crude oil could simulate higher ROS levels and subsequently cause much severer oxidative damage and cellular apoptosis than crude oil alone on sea cucumber.

Sea cucumber (Apostichopus japonicus) is mainly cultured in the coastal zone, where it is easily threatened by accidental oil spills. Chemical dispersant is one of the efficient oil spill responses for mitigating the overall environmental damage of oil spills. However, the impact of crude oil and chemical dispersants on sea cucumber is less well known. Hence, the present study focused on exploring the antioxidant response and oxidative stress in the respiratory tree of sea cucumber following exposure to GM-2 chemical dispersant (DISP), water-accommodated fractions (WAF), and chemically enhanced WAF (CEWAF) of Oman crude oil for 24 h. Results manifested that WAF exposure caused a significant increase in the reactive oxygen species (ROS) level (5.29 ± 0.30 AU·mgprot⁻¹), and the effect was much more obvious in CEWAF treatment (5.73 ± 0.16 AU·mgprot⁻¹). Total antioxidant capacity (T-AOC), as an important biomarker of the antioxidant defense capacity, showed an increasing trend following WAF exposure (0.95 ± 0.12 U·mgprot⁻¹) while a significant reduction in T-AOC was observed following CEWAF exposure (0.23 ± 0.13 U·mgprot⁻¹). Moreover, we also evaluated the oxidative damage of the macromolecules (DNA, protein, and lipid), and our results revealed that the presence of chemical dispersant enhanced oxidative damage caused by crude oil to sea cucumber.

The formation of sunken oils is mainly dominated by the interaction between spilled oils and sediments. Due to their patchiness and invisibility, cleaning operations become difficult. As a result, sunken oils may cause long-term and significant damage to marine benthonic organisms. In the present study, a bench experiment was designed and conducted to investigate the quantitative distribution of polycyclic aromatic hydrocarbons (PAHs) in sunken oils in the presence of chemical dispersant and sediment. The oil sinking efficiency (OSE) of 16 priority total PAHs in the sediment phase was analyzed with different dosages of dispersant. The results showed that the synergistic effect of chemical dispersant and sediment promoted the formation of sunken oils, and the content of PAHs partitioned in the sunken oils increased with the increase of dispersant-to-oil ratios (DORs). Furthermore, with the addition of chemical dispersant, due to the solubility and hydrophobicity of individual PAHs, the high molecular weight (HMW) PAHs with 4–6 rings tended to partition to sediment compared with low molecular weight (LMW) PAHs with 2–3 rings. The synergistic effect of chemical dispersant and sediment could enhance the OSE of HMW PAHs in sunken oils, which might subsequently cause certain risks for marine benthonic organisms.