Maize is a critical crop for food, feed, and bioenergy worldwide. This study characterized the genetic diversity and population structure of 212 important inbred lines collected from the Southeast China breeding program using the Maize6H-60K single nucleotide polymorphism (SNP) array. To investigate the genetic architecture of plant height (PH) and ear height (EH), genome-wide association analysis (GWAS) was performed on this population in 2021 and 2022. Cluster analysis and population genetic structure analysis grouped the 212 maize inbred lines into 10 distinct categories. GWAS identified significant associations for PH, EH, and the EH/PH ratio. A total of 40 significant SNP (p < 8.55359 × 10⁻⁷) were detected, including nine associated with PH, with phenotypic variation explained (PVE) ranging from 3.42% to 25.92%. Additionally, 16 SNP were linked to EH, with PVE ranging from 2.49% to 38.49%, and 15 SNP were associated with the EH/PH ratio, showing PVE between 3.43% and 16.83%. Five stable SNP, identified across two or more environments, were further analyzed. Three of these SNP loci are reported for the first time in this study: two loci associated with the PH, AX-108020973, and AX-108022922, as well as one new locus, AX-108096437, which was significantly associated with the EH/PH ratio. Additionally, two other significant SNP (AX-247241325 and AX-108097244) were located within a 2 Mb range of previously identified QTL and/or related SNP. Within the 200 kb confidence intervals of these five stable SNP loci, 76 functionally annotated genes were identified. Further functional analysis indicated that 14 of these genes may play a role in regulating plant morphology, which is primarily involved in hormone synthesis, microtubule development, root growth, and cell division regulation. For instance, the homologous genes GRMZM2G375249 and GRMZM2G076029 in maize correspond to OsPEX1 in rice, a protein similar to extension proteins that are implicated in lignin biosynthesis, plant growth promotion, and the negative regulation of root growth through gibberellin-mediated pathways. The candidate gene corresponding to AX-108097244 is GRMZM2G464754; previous studies have reported its involvement in regulating EH in maize. These findings enhance the understanding of QTL associated with maize plant-type traits and provide a foundation for cloning PH, EH-related genes. Therefore, the results also support the development of functional markers for target genes and the breeding of improved maize varieties.

Seed reserve mobilization is a crucial physiological process during seed germination. Enhancing the reserve mobilization in sweet corn is vital for seed germination and seedling growth. In this study, a genome-wide association analysis (GWAS) was conducted to predict candidate genes for regulating the weight of mobilized reserved seeds (WMRS) and kernel weight (KW) in sweet corn. GWAS was performed using the BLINK model with the maize 56K SNP chip. The results indicated that there was a positive correlation between the WMRS and KW, with coefficients of variation of 68.18% and 44.63%. Association analysis identified thirteen SNPs associated with two traits, and linkage disequilibrium analysis revealed that eight of these SNPs were in strong linkage. A total of 298 candidate genes were identified within the confidence interval, of which 79 were annotated. About 20 candidate genes were identified through the comparison of homologous genes in Arabidopsis. These genes were enriched in regulating ribosome biogenesis, signal transduction, hormone synthesis, and RNA degradation processes. This study provides important insights into the genetic mechanisms governing germination traits in sweet corn, aiding further research into the localization and cloning of genes involved in the mobilization of reserve materials.

Sweet corn is highly susceptible to low temperatures, especially during seed germination, which severely affects plant growth and crop yield. This study used 100 sweet corn micro-core germplasms to evaluate two key germination traits under cold stress: seed storage material utilization efficiency (SRUE) and mobilization weight (WMSR). To investigate the genetic basis of cold germination in sweet corn, we selected the BLINK model for GWAS due to its ability to minimize false positives. A total of nine SNPs were found to be significantly associated with cold germination. These SNPs explained between 9.8% and 17.2% of the phenotypic variance (PVE). Within the confidence interval, 63 functionally annotated genes were identified. Fourteen candidate genes associated with cold germination were identified through GO functional analysis and the functional expression of homologous genes. A literature analysis indicated that these genes are primarily involved in seed germination, cold tolerance, and responses to other abiotic stresses. These findings enhance our understanding of the genetic and molecular mechanisms underlying cold germination, establishing a theoretical foundation for breeding cold-tolerant sweet corn varieties.

Seed germination is the initial and critical stage of plant growth and development. This study conducted a genome-wide association study (GWAS) on seedling

Salinization stress poses a major environmental factor that adversely affects maize (Zea mays L.) growth and development. Thus, identifying and utilizing alkaline tolerance-related genes in maize is crucial for enhancing resistance to alkaline stress. In this study, a genome-wide association study (GWAS) was conducted to analyze alkali tolerance in seedlings, focusing on biomass-related traits at the seedling stage across a panel of 212 maize inbred lines. The analysis found nine single-nucleotide polymorphism (SNP) loci significantly associated with alkali tolerance during the seedling stage. Within the confidence intervals of these loci, 57 genes with clear functional annotations were identified, among which eight were predicted to be involved in alkali tolerance based on functional annotation and homology analysis. qRT-PCR expression validation of selected candidate genes revealed that the relative expression level of GRMZM2G028089 was similar between in L99 and M-J244-3 lines. In contrast, the expression levels of GRMZM2G071196, GRMZM2G313162 and GRMZM5G883126 were higher in the L99 line compared to M-J244-3, suggesting their potential positive regulatory roles in the response to alkaline stress. These findings provide important theoretical support for the targeted breeding of alkali-resistant maize varieties.

Sweet corn is significantly influenced by climatic variability and planting geometry in account of its high sugar content and consumer appeal. Therefore, this study was conducted to evaluate the effects of four sowing dates i.e. 25th April, 10th May, 25th May and 10th June and three plant...