The aim of this study was to evaluate the in vitro and in vivo efficacy of propolis against Ligictaluridus floridanus on Ictalurus punctatus gills. This st

The habitat characteristics preferred by Penaeus shrimp inhabiting positive (freshwater-dominated) estuaries in North America have been scarcely investigated. Identifying the main environmental factors within the estuary that affect shrimp abundance is relevant to understanding habitat utilization within this particular nursery habitat. This study aimed to evaluate the distribution of penaeid shrimp species along the Soto La Marina River estuary (Northeastern Mexico), analyzing the influence of abiotic factors (water temperature, salinity, and dissolved oxygen) and submerged aquatic vegetation biomass on shrimp populations. A total of 1069 shrimp belonging to four penaeid species (Penaeus aztecus, P. duorarum, P. brasiliensis, and P. setiferus) were collected. Except for P. setiferus, the highest shrimp abundance was near the mouth of the estuary, where the highest salinity and seagrass biomass (Halodule wrightii) were recorded. Salinity and seagrass biomass were the main factors influencing penaeids’ spatial preferences along the estuary, showing significant positive relationships with shrimp abundance in most of the cases examined. Penaeus setiferus was the only species exhibiting peak abundance in an estuarine area devoid of aquatic vegetation (without seagrass or macroalgae) and with a significantly lower salinity level (ca. 22 ppt) than the levels recorded near the river mouth (ca. 28–32 ppt). Differences in osmotic capacity between the species, the protective function of seagrasses against currents, and their role as feeding and refuge habitats could explain the observed spatial distribution in the estuary.

Growth rates of the brown shrimp Penaeus aztecus Ives, 1891 and pink shrimp Penaeus duorarumBurkenroad, 1931 were estimated using length-frequency data in the hypersaline coastal lagoon, Laguna Madre of Tamaulipas, Mexico. The influence of environmental variables (salinity and temperature) and shrimp body size on the growth of both penaeid species were also evaluated. Shrimps were collected monthly from two fishing zones located at the central part of the lagoon throughout one year. A total of 4458 shrimps were analysed (69.3% P. aztecus and 30.7% P. duorarum) with total lengths (TL) ranging from 50.9 to 131.4 mm in P. aztecus and 59.7 to 134.5 mm in P. duorarum. Mean growth rate (±SD) of P. duorarum (3.10 ±1.78 mm TL per week) was slightly higher than that of P. aztecus (2.71 ±1.37 mm TL per week); however, the interspecific difference was not significant (p>0.05). Growth rates for both the penaeid shrimp species increased as water temperature increased. Additionally, a negative relationship between the growth rate and body length of shrimp was also detected suggesting that the positive effect of water temperature on the increase in shrimp length may be size-dependent.

The emergence of multidrug-resistant (MDR) bacteria has increased the need for alternative antimicrobial agents from natural sources. Propolis, a resinous bee product produced by honey bees (Apis mellifera) from plant resins, is rich in bioactive compounds with recognized antimicrobial and antioxidant properties. This study evaluated the phytochemical composition, antioxidant capacity, and antibacterial activity of propolis extracts obtained using ethanol, methanol, acetone, and water against clinically relevant MDR bacterial isolates. Total phenolic content was determined using the Folin–Ciocalteu assay, total flavonoid content by the aluminum chloride colorimetric method, and condensed tannin content by the butanol–HCl assay. Antioxidant activity was assessed using the 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) assays. Antibacterial activity was evaluated through minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), MBC/MIC ratio, biofilm formation inhibition, and bacterial growth inhibition assays against MDR isolates of Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus faecium, and Enterobacter cloacae. Significant differences among extraction solvents were observed for all phytochemical and antioxidant parameters (p < 0.0001). Ethanolic extracts exhibited the highest concentrations of total phenolics, flavonoids, and condensed tannins, as well as the greatest antioxidant activity. MIC values ranged from 3.13 to 25 mg/mL, whereas MBC values ranged from 6.25 to >50 mg/mL depending on bacterial species and extraction solvent. E. coli and E. faecium were the most susceptible isolates, while P. aeruginosa and E. cloacae showed greater tolerance. Most MBC/MIC ratios indicated bactericidal activity. Biofilm inhibition was significantly affected by bacterial strain and extraction solvent (p < 0.001), with ethanolic extracts producing the highest inhibition percentages. These findings demonstrate that ethanolic propolis extracts exhibit strong antioxidant activity, as well as bacteriostatic, bactericidal, and biofilm-formation-inhibitory effects, supporting their potential as complementary antimicrobial agents within a One Health framework.

Las infecciones parasitarias representan una limitante sanitaria clave en la producción acuícola intensiva, especialmente en etapas tempranas del ciclo de vida de los peces. La alta densidad, el estrés ambiental y la inmadurez inmunológica aumentan la susceptibilidad a protozoarios y helmintos con ciclos directos o indirectos. Esta revisión analiza los principales grupos de parásitos presentes en hatcheries (criaderos de peces) y sistemas de cría: ciliados, dinoflagelados, monogéneos, mixosporidios, tremátodos digéneos y céstodos. Se examinan su prevalencia, estacionalidad, transmisión y efectos patológicos en alevines, crías y reproductores. Los sistemas cerrados reducen el riesgo de parásitos heteroxenos, mientras que los estanques rústicos favorecen su transmisión por hospederos intermediarios. La interacción entre ambiente y densidad determina la expresión clínica y el impacto productivo. El monitoreo epidemiológico y el conocimiento de los ciclos biológicos son esenciales para estrategias preventivas eficaces.