The available energy can be effectively upgraded by adopting smart energy conversion measures. The biodegradability of biomass can be improved by employing pretreatment techniques; however, such methods result in reduced energy efficiency. In this study, microwave (MW) irradiation is used for green algae (Enteromorpha) pretreatment in combination with iron oxide nanoparticles (NPs) which act as a heterogeneous catalyst during anaerobic digestion process for biogas enhancement. Batch-wise anaerobic digestion was carried out. The results showed that MW pretreatment and its combination with Fe₃O₄ NPs produced highest yields of biogas and hydrogen as compared to the individual ones and control. The biogas amount and hydrogen % v/v achieved by MW pretreatment + Fe₃O₄ NPs group were 328 mL and 51.5%, respectively. The energy analysis indicated that synergistic application of MW pretreatment with Fe₃O₄ NPs produced added energy while consuming less input energy than MW pretreatment alone. The kinetic parameters of the reaction were scientifically evaluated by using modified Gompertz and Logistic function model for each experimental case. MW pretreatment + Fe₃O₄ NPs group improved biogas production potential and maximum biogas production rate.