Enzymatic luminescent systems are a promising tool for rapid detection of heavy metals ions for water quality assessment. Nevertheless, their widespread use is limited by the lack of test procedure automation and available sensitive handheld luminometers. Herein we describe integration of disposable microfluidic chips for bioluminescent enzyme-inhibition based assay with a handheld luminometer, which detection system is based on a thermally stabilized silicon photomultiplier (SiPM). Microfluidic chips were made of poly(methyl methacrylate) by micro-milling method and sealed using a solvent bonding technique. The composition of the bioluminescent system in microfluidic chip was optimized to achieve higher luminescence intensity and storage time. Results indicate that developed device provided comparable sensitivity with bench-scale PMT-based commercial luminometers. Limit of detection for copper (II) sulfate reached 2.5 mg/L for developed biosensor. Hereby we proved the concept of handheld enzymatic optical biosensors with disposable chips for bioassay. The proposed biosensor can be used as an early warning field-deployable system for rapid detection of heavy metals salts and other toxic chemicals, which affect bioluminescent signal of enzymatic reaction.

The essential advantages of microfluidic studies are the excellent visualization of the processes of oil displacement from the porous medium model, simple cleaning, and the possibility of the repeated use of the microfluidic chip. The present article deals with the process of oil displacement by suspension flooding using a microfluidic chip, simulating a porous medium, and the suspensions of silicon dioxide nanoparticles (22 nm). The mass concentration of nanoparticles in suspensions ranged from 0.1 to 2 wt%. Five mass concentrations (0.125 wt%, 0.25 wt%, 0.5 wt%, 1 wt% and 2 wt%) were considered. The article presents the experimental photographs of the oil displacement process by water and SiO₂ suspension. It is shown that, with the increasing concentration of nanoparticles, the oil recovery factor increases. A significant effect is observed at 0.5 wt% concentration of nanoparticles. It is shown that the increase in oil recovery during flooding by SiO₂ suspension with the maximum concentration was 16%.

A number of approaches have been proposed and tested to transfer enzymatic reactions into the functional elements of microfluidic chips on the example of the bienzyme bioluminescent reaction...

The rapid detection of pollutants in water can be performed with enzymatic probes, the catalytic light-emitting activity of which decreases in the presence of many types of pollutants. Herein, we present a microfluidic system for continuous chemoenzymatic biosensing that generates emulsion droplets containing two enzymes of the bacterial bioluminescent system (luciferase and NAD(P)H:FMN–oxidoreductase) with substrates required for the reaction. The developed chip generates “water-in-oil” emulsion droplets with a volume of 0.1 $\mathsf{\mu }$L and a frequency of up to 12 drops per minute as well as provides the efficient mixing of reagents in droplets and their distancing. The bioluminescent signal from each individual droplet was measured by a photomultiplier tube with a signal-to-noise ratio of up to 3000/1. The intensity of the luminescence depended on the concentration of the copper sulfate with the limit of its detection of 5 $\mathsf{\mu }$M. It was shown that bioluminescent enzymatic reactions could be carried out in droplet reactors in dispersed streams. The parameters and limitations required for the bioluminescent reaction to proceed were also studied. Hereby, chemoenzymatic sensing capabilities powered by a droplet microfluidics manipulation technique may serve as the basis for early-warning online water pollution systems.

This article presents a new approach to determining the viscosity of Newtonian fluid. The approach is based on the analysis of the secondary Dean flow in a curved channel. The study of the flow patterns of water and aqueous solutions of glycerin in a microfluidic chip with a U-microchannel was carried out. The advantages of a microfluidic viscometer based on a secondary Dean flow are its simplicity, quickness, and high accuracy in determining the viscosity coefficient of a liquid. A viscosity image in a short movie represents fluid properties. It is revealed that the viscosity coefficient can be determined by the dependence of the recirculation angle of the secondary Dean flow. The article provides a correlation between the Dean number and the flow recirculation angle. The results of the field experiment, presented in the article, correlate with the data obtained using computational fluid dynamics and allow for selecting parameters to create microfluidic viscometers with a U-shaped microchannel.