Body temperature is an important indicator that may indicate the possibility of the existence of various pathological conditions and diseases. In the head and neck area, an infrared camera allows accurate temperature measurements of all regions of interest. The analysis of temperature characteristics of the region of interest of the head and neck in healthy subjects in terms of comparison of values in relation to the side of the face in the same person, and the comparison of values relative to the sex of the subjects is the topic of this research. These analyses are performed to create temperature maps of the face and determine physiological values. The research was conducted with the participation of 30 healthy people, 16 women and 14 men of different ages. Thermal imaging was performed in controlled conditions with infrared thermographic camera Varioscan 3021ST, while the software package IRBIS Professional 2.2 was used for thermogram analysis. Results show that the temperatures in female subjects at the submandibular region are significantly lower than in male subjects with an average temperature difference of 0.46?C, and the temperatures in female subjects at the supraorbital region are on average 0.5?C higher than in male subjects.