Carlos Murillo Almuzara

Carlos Murillo Almuzara

Blusense Diagnostics, Copenhagen, Hovedstaden, Denmark
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Research Focus & Metrics

Academic Career Length

6Years (2021-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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29 January 2021 in Sensors
Verified
Journal Article
Open access
PDF file
ACAlessandra Camarca
Alessandra Camarca
Antonio VarrialeAntonio Varriale
Antonio Varriale
ACAlessandro Capo
Alessandro Capo
APAngela Pennacchio
Angela Pennacchio
Alessia CalabreseAlessia Calabrese
Alessia Calabrese
Maria StaianoMaria Staiano
Maria Staiano
The purpose of this work is to provide an exhaustive overview of the emerging biosensor technologies for the detection of analytes of interest for food, environment, security, and health. Over the years, biosensors have acquired increasing importance in a wide range of applications due to synergistic studies of various scientific disciplines, determining their great commercial potential and revealing how nanotechnology and biotechnology can be strictly connected. In the present scenario, biosensors have increased their detection limit and sensitivity unthinkable until a few years ago. The most widely used biosensors are optical-based devices such as surface plasmon resonance (SPR)-based biosensors and fluorescence-based biosensors. Here, we will review them by highlighting how the progress in their design and development could impact our daily life.

Verified
Journal Article
Open access
PDF file
Giulia RuscianoGiulia Rusciano
Giulia Rusciano
ACAngela Capaccio
Angela Capaccio
ASAntonio Sasso
Antonio Sasso
ACAlessandro Capo
Alessandro Capo
CACarlos Murillo Almuzara
Carlos Murillo Almuzara
Antonio VarrialeAntonio Varriale
Antonio Varriale
The development of sensitive methods for the detection of endotoxin molecules, such as lipopolysaccharides (LPS), is essential for food safety and health control. Conventional analytical methods used for LPS detection are based on the pyrogen test, plating and culture-based methods, and the limulus amoebocyte lysate method (LAL). Alternatively, the development of reliable biosensors for LPS detection would be highly desirable to solve some critical issues, such as high cost and a long turnaround time. In this work, we present a label-free Surface-Enhanced Raman Spectroscopy (SERS)-based method for LPS detection in its free form. The proposed method combines the benefits of plasmonic enhancement with the selectivity provided by a specific anti-lipid A antibody (Ab). A high-enhancing nanostructured silver substrate was coated with Ab. The presence of LPS was quantitatively monitored by analyzing the changes in the Ab spectra obtained in the absence and presence of LPS. A limit of detection (LOD) and quantification (LOQ) of 12 ng/mL and 41 ng/mL were estimated, respectively. Importantly, the proposed technology could be easily expanded for the determination of other biological macromolecules.