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Dr. Antonio Varriale
Institute of Food Science, Italian National Research Council (CNR), 00185 Rome, Italy

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0 Biosensors
0 Photonics
0 Antibody
0 Recombinant
0 thermostable

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Review
Published: 29 January 2021 in Sensors
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The purpose of this work is to provide an exhaustive overview of the emerging biosensor technologies for the detection of analytes of interest for food, environment, security, and health. Over the years, biosensors have acquired increasing importance in a wide range of applications due to synergistic studies of various scientific disciplines, determining their great commercial potential and revealing how nanotechnology and biotechnology can be strictly connected. In the present scenario, biosensors have increased their detection limit and sensitivity unthinkable until a few years ago. The most widely used biosensors are optical-based devices such as surface plasmon resonance (SPR)-based biosensors and fluorescence-based biosensors. Here, we will review them by highlighting how the progress in their design and development could impact our daily life.

ACS Style

Alessandra Camarca; Antonio Varriale; Alessandro Capo; Angela Pennacchio; Alessia Calabrese; Cristina Giannattasio; Carlos Murillo Almuzara; Sabato D’Auria; Maria Staiano. Emergent Biosensing Technologies Based on Fluorescence Spectroscopy and Surface Plasmon Resonance. Sensors 2021, 21, 906 .

AMA Style

Alessandra Camarca, Antonio Varriale, Alessandro Capo, Angela Pennacchio, Alessia Calabrese, Cristina Giannattasio, Carlos Murillo Almuzara, Sabato D’Auria, Maria Staiano. Emergent Biosensing Technologies Based on Fluorescence Spectroscopy and Surface Plasmon Resonance. Sensors. 2021; 21 (3):906.

Chicago/Turabian Style

Alessandra Camarca; Antonio Varriale; Alessandro Capo; Angela Pennacchio; Alessia Calabrese; Cristina Giannattasio; Carlos Murillo Almuzara; Sabato D’Auria; Maria Staiano. 2021. "Emergent Biosensing Technologies Based on Fluorescence Spectroscopy and Surface Plasmon Resonance." Sensors 21, no. 3: 906.

Journal article
Published: 26 September 2020 in International Journal of Molecular Sciences
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The synthesis of two 5′-end (4-dimethylamino)azobenzene conjugated G-quadruplex forming aptamers, the thrombin binding aptamer (TBA) and the HIV-1 integrase aptamer (T30695), was performed. Their structural behavior was investigated by means of UV, CD, fluorescence spectroscopy, and gel electrophoresis techniques in K+-containing buffers and water-ethanol blends. Particularly, we observed that the presence of the 5′-(4-dimethylamino)azobenzene moiety leads TBA to form multimers instead of the typical monomolecular chair-like G-quadruplex and almost hampers T30695 G-quadruplex monomers to dimerize. Fluorescence studies evidenced that both the conjugated G-quadruplexes possess unique fluorescence features when excited at wavelengths corresponding to the UV absorption of the conjugated moiety. Furthermore, a preliminary investigation of the trans-cis conversion of the dye incorporated at the 5′-end of TBA and T30695 showed that, unlike the free dye, in K+-containing water-ethanol-triethylamine blend the trans-to-cis conversion was almost undetectable by means of a standard UV spectrophotometer.

ACS Style

Concetta Imperatore; Antonio Varriale; Elisa Rivieccio; Angela Pennacchio; Maria Staiano; Sabato D’Auria; Marcello Casertano; Carlo Altucci; MohammadHassan Valadan; Manjot Singh; Marialuisa Menna; Michela Varra. Spectroscopic Properties of Two 5′-(4-Dimethylamino)Azobenzene Conjugated G-Quadruplex Forming Oligonucleotides. International Journal of Molecular Sciences 2020, 21, 7103 .

AMA Style

Concetta Imperatore, Antonio Varriale, Elisa Rivieccio, Angela Pennacchio, Maria Staiano, Sabato D’Auria, Marcello Casertano, Carlo Altucci, MohammadHassan Valadan, Manjot Singh, Marialuisa Menna, Michela Varra. Spectroscopic Properties of Two 5′-(4-Dimethylamino)Azobenzene Conjugated G-Quadruplex Forming Oligonucleotides. International Journal of Molecular Sciences. 2020; 21 (19):7103.

Chicago/Turabian Style

Concetta Imperatore; Antonio Varriale; Elisa Rivieccio; Angela Pennacchio; Maria Staiano; Sabato D’Auria; Marcello Casertano; Carlo Altucci; MohammadHassan Valadan; Manjot Singh; Marialuisa Menna; Michela Varra. 2020. "Spectroscopic Properties of Two 5′-(4-Dimethylamino)Azobenzene Conjugated G-Quadruplex Forming Oligonucleotides." International Journal of Molecular Sciences 21, no. 19: 7103.

Journal article
Published: 15 September 2019 in Sensors
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In this paper we introduce a field diagnostic device based on the combination of advanced bio-sensing and photonics technologies, to tackle emerging and endemic viruses causing swine epidemics, and consequently significant economic damage in farms. The device is based on the use of microring resonators fabricated in silicon nitride with CMOS compatible techniques. In the paper, the designed and fabricated photonic integrated circuit (PIC) sensors are presented and characterized, showing an optimized performance in terms of optical losses (30 dB per ring) and extinction ration for ring resonances (15 dB). Furthermore, the results of an experiment for porcine circovirus 2 (PCV2) detection by using the developed biosensors are presented. Positive detection for different virus concentrations has been obtained. The device is currently under development in the framework of the EU Commission co-funded project SWINOSTICS.

ACS Style

Amadeu Griol; Sergio Peransi; Manuel Rodrigo; Juan Hurtado; Laurent Bellieres; Teodora Ivanova; David Zurita; Carles Sánchez; Sara Recuero; Alejandro Hernández; Santiago Simón; Gyula Balka; Ioannis Bossis; Alessandro Capo; Alessandra Camarca; Sabato D’Auria; Antonio Varriale; Alessandro Giusti. Design and Development of Photonic Biosensors for Swine Viral Diseases Detection. Sensors 2019, 19, 3985 .

AMA Style

Amadeu Griol, Sergio Peransi, Manuel Rodrigo, Juan Hurtado, Laurent Bellieres, Teodora Ivanova, David Zurita, Carles Sánchez, Sara Recuero, Alejandro Hernández, Santiago Simón, Gyula Balka, Ioannis Bossis, Alessandro Capo, Alessandra Camarca, Sabato D’Auria, Antonio Varriale, Alessandro Giusti. Design and Development of Photonic Biosensors for Swine Viral Diseases Detection. Sensors. 2019; 19 (18):3985.

Chicago/Turabian Style

Amadeu Griol; Sergio Peransi; Manuel Rodrigo; Juan Hurtado; Laurent Bellieres; Teodora Ivanova; David Zurita; Carles Sánchez; Sara Recuero; Alejandro Hernández; Santiago Simón; Gyula Balka; Ioannis Bossis; Alessandro Capo; Alessandra Camarca; Sabato D’Auria; Antonio Varriale; Alessandro Giusti. 2019. "Design and Development of Photonic Biosensors for Swine Viral Diseases Detection." Sensors 19, no. 18: 3985.

Journal article
Published: 01 March 2019 in Cell Communication and Signaling
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Shp1, a tyrosine-phosphatase-1 containing the Src-homology 2 (SH2) domain, is involved in inflammatory and immune reactions, where it regulates diverse signalling pathways, usually by limiting cell responses through dephosphorylation of target molecules. Moreover, Shp1 regulates actin dynamics. One Shp1 target is Src, which controls many cellular functions including actin dynamics. Src has been previously shown to be activated by a signalling cascade initiated by the cytosolic-phospholipase A2 (cPLA2) metabolite glycerophosphoinositol 4-phosphate (GroPIns4P), which enhances actin polymerisation and motility. While the signalling cascade downstream Src has been fully defined, the mechanism by which GroPIns4P activates Src remains unknown. Affinity chromatography, mass spectrometry and co-immunoprecipitation studies were employed to identify the GroPIns4P-interactors; among these Shp1 was selected for further analysis. The specific Shp1 residues interacting with GroPIns4P were revealed by NMR and validated by site-directed mutagenesis and biophysical methods such as circular dichroism, isothermal calorimetry, fluorescence spectroscopy, surface plasmon resonance and computational modelling. Morphological and motility assays were performed in NIH3T3 fibroblasts. We find that Shp1 is the direct cellular target of GroPIns4P. GroPIns4P directly binds to the Shp1-SH2 domain region (with the crucial residues being Ser 118, Arg 138 and Ser 140) and thereby promotes the association between Shp1 and Src, and the dephosphorylation of the Src-inhibitory phosphotyrosine in position 530, resulting in Src activation. As a consequence, fibroblast cells exposed to GroPIns4P show significantly enhanced wound healing capability, indicating that GroPIns4P has a stimulatory role to activate fibroblast migration. GroPIns4P is produced by cPLA2 upon stimulation by diverse receptors, including the EGF receptor. Indeed, endogenously-produced GroPIns4P was shown to mediate the EGF-induced cell motility. This study identifies a so-far undescribed mechanism of Shp1/Src modulation that promotes cell motility and that is dependent on the cPLA2 metabolite GroPIns4P. We show that GroPIns4P is required for EGF-induced fibroblast migration and that it is part of a cPLA2/GroPIns4P/Shp1/Src cascade that might have broad implications for studies of immune-inflammatory response and cancer.

ACS Style

Alessia Varone; Stefania Mariggiò; Manpreet Patheja; Vincenzo Maione; Antonio Varriale; Mariangela Vessichelli; Daniela Spano; Fabio Formiggini; Matteo Lo Monte; Nadia Brancati; Maria Frucci; Pompea Del Vecchio; Sabato D’Auria; Angela Flagiello; Clara Iannuzzi; Alberto Luini; Piero Pucci; Lucia Banci; Carmen Valente; Daniela Corda. A signalling cascade involving receptor-activated phospholipase A2, glycerophosphoinositol 4-phosphate, Shp1 and Src in the activation of cell motility. Cell Communication and Signaling 2019, 17, 20 .

AMA Style

Alessia Varone, Stefania Mariggiò, Manpreet Patheja, Vincenzo Maione, Antonio Varriale, Mariangela Vessichelli, Daniela Spano, Fabio Formiggini, Matteo Lo Monte, Nadia Brancati, Maria Frucci, Pompea Del Vecchio, Sabato D’Auria, Angela Flagiello, Clara Iannuzzi, Alberto Luini, Piero Pucci, Lucia Banci, Carmen Valente, Daniela Corda. A signalling cascade involving receptor-activated phospholipase A2, glycerophosphoinositol 4-phosphate, Shp1 and Src in the activation of cell motility. Cell Communication and Signaling. 2019; 17 (1):20.

Chicago/Turabian Style

Alessia Varone; Stefania Mariggiò; Manpreet Patheja; Vincenzo Maione; Antonio Varriale; Mariangela Vessichelli; Daniela Spano; Fabio Formiggini; Matteo Lo Monte; Nadia Brancati; Maria Frucci; Pompea Del Vecchio; Sabato D’Auria; Angela Flagiello; Clara Iannuzzi; Alberto Luini; Piero Pucci; Lucia Banci; Carmen Valente; Daniela Corda. 2019. "A signalling cascade involving receptor-activated phospholipase A2, glycerophosphoinositol 4-phosphate, Shp1 and Src in the activation of cell motility." Cell Communication and Signaling 17, no. 1: 20.

Concept paper
Published: 20 January 2019 in Sensors
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In this paper, we present the concept of a novel diagnostic device for on-site analyses, based on the use of advanced bio-sensing and photonics technologies to tackle emerging and endemic viruses causing swine epidemics and significant economic damage in farms. The device is currently under development in the framework of the EU Commission co-funded project. The overall concept behind the project is to develop a method for an early and fast on field detection of selected swine viruses by non-specialized personnel. The technology is able to detect pathogens in different types of biological samples, such as oral fluids, faeces, blood or nasal swabs. The device will allow for an immediate on-site threat assessment. In this work, we present the overall concept of the device, its architecture with the technical requirements, and all the used innovative technologies that contribute to the advancements of the current state of the art.

ACS Style

Concetta Montagnese; Paolo Barattini; Alessandro Giusti; Gyula Balka; Ugo Bruno; Ioannis Bossis; Athanasios Gelasakis; Matteo Bonasso; Panayiotis Philmis; Lilla Dénes; Sergio Peransi; Manuel Rodrigo; Santiago Simón; Amadeu Griol; Grzegorz Wozniakowski; Katarzyna Podgorska; Carolina Pugliese; Lapo Nannucci; Sabato D’Auria; Antonio Varriale. A Diagnostic Device for In-Situ Detection of Swine Viral Diseases: The SWINOSTICS Project. Sensors 2019, 19, 407 .

AMA Style

Concetta Montagnese, Paolo Barattini, Alessandro Giusti, Gyula Balka, Ugo Bruno, Ioannis Bossis, Athanasios Gelasakis, Matteo Bonasso, Panayiotis Philmis, Lilla Dénes, Sergio Peransi, Manuel Rodrigo, Santiago Simón, Amadeu Griol, Grzegorz Wozniakowski, Katarzyna Podgorska, Carolina Pugliese, Lapo Nannucci, Sabato D’Auria, Antonio Varriale. A Diagnostic Device for In-Situ Detection of Swine Viral Diseases: The SWINOSTICS Project. Sensors. 2019; 19 (2):407.

Chicago/Turabian Style

Concetta Montagnese; Paolo Barattini; Alessandro Giusti; Gyula Balka; Ugo Bruno; Ioannis Bossis; Athanasios Gelasakis; Matteo Bonasso; Panayiotis Philmis; Lilla Dénes; Sergio Peransi; Manuel Rodrigo; Santiago Simón; Amadeu Griol; Grzegorz Wozniakowski; Katarzyna Podgorska; Carolina Pugliese; Lapo Nannucci; Sabato D’Auria; Antonio Varriale. 2019. "A Diagnostic Device for In-Situ Detection of Swine Viral Diseases: The SWINOSTICS Project." Sensors 19, no. 2: 407.

Concept paper
Published: 21 December 2018 in Sensors
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In this paper, we present WaterSpy, a project developing an innovative, compact, cost-effective photonic device for pervasive water quality sensing, operating in the mid-IR spectral range. The approach combines the use of advanced Quantum Cascade Lasers (QCLs) employing the Vernier effect, used as light source, with novel, fibre-coupled, fast and sensitive Higher Operation Temperature (HOT) photodetectors, used as sensors. These will be complemented by optimised laser driving and detector electronics, laser modulation and signal conditioning technologies. The paper presents the WaterSpy concept, the requirements elicited, the preliminary architecture design of the device, the use cases in which it will be validated, while highlighting the innovative technologies that contribute to the advancement of the current state of the art.

ACS Style

Nikolaos Doulamis; Athanasios Voulodimos; Anastasios Doulamis; Matthaios Bimpas; Aikaterini Angeli; Nikolaos Bakalos; Alessandro Giusti; Panayiotis Philimis; Antonio Varriale; Alessio Ausili; Sabato D’Auria; George Lampropoulos; Matthias Baer; Bernhard Schmauss; Stephan Freitag; Bernhard Lendl; Krzysztof Młynarczyk; Aleksandra Sosna-Głębska; Artur Trajnerowicz; Jarosław Pawluczyk; Mateusz Żbik; Jacek Kułakowski; Panagiotis Georgiadis; Stéphane Blaser; Nicola Bazzurro. WaterSpy: A High Sensitivity, Portable Photonic Device for Pervasive Water Quality Analysis. Sensors 2018, 19, 33 .

AMA Style

Nikolaos Doulamis, Athanasios Voulodimos, Anastasios Doulamis, Matthaios Bimpas, Aikaterini Angeli, Nikolaos Bakalos, Alessandro Giusti, Panayiotis Philimis, Antonio Varriale, Alessio Ausili, Sabato D’Auria, George Lampropoulos, Matthias Baer, Bernhard Schmauss, Stephan Freitag, Bernhard Lendl, Krzysztof Młynarczyk, Aleksandra Sosna-Głębska, Artur Trajnerowicz, Jarosław Pawluczyk, Mateusz Żbik, Jacek Kułakowski, Panagiotis Georgiadis, Stéphane Blaser, Nicola Bazzurro. WaterSpy: A High Sensitivity, Portable Photonic Device for Pervasive Water Quality Analysis. Sensors. 2018; 19 (1):33.

Chicago/Turabian Style

Nikolaos Doulamis; Athanasios Voulodimos; Anastasios Doulamis; Matthaios Bimpas; Aikaterini Angeli; Nikolaos Bakalos; Alessandro Giusti; Panayiotis Philimis; Antonio Varriale; Alessio Ausili; Sabato D’Auria; George Lampropoulos; Matthias Baer; Bernhard Schmauss; Stephan Freitag; Bernhard Lendl; Krzysztof Młynarczyk; Aleksandra Sosna-Głębska; Artur Trajnerowicz; Jarosław Pawluczyk; Mateusz Żbik; Jacek Kułakowski; Panagiotis Georgiadis; Stéphane Blaser; Nicola Bazzurro. 2018. "WaterSpy: A High Sensitivity, Portable Photonic Device for Pervasive Water Quality Analysis." Sensors 19, no. 1: 33.

Proceedings article
Published: 01 January 2018 in Advanced Photonics
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A simple approach to low-cost plasmonic sensing is obtained exploiting Plastic Optical Fibers (POFs). POFs are especially advantageous for their properties and, when receptors are used for bio/chemicals detection, are suitable for different application fields.

ACS Style

Nunzio Cennamo; Sabato D'auria; Antonio Varriale; Maria Pesavento; Luigi Zeni. Plasmonic Chemical and Biological Sensors based on plastic optical fibers. Advanced Photonics 2018, SeW1E.1 .

AMA Style

Nunzio Cennamo, Sabato D'auria, Antonio Varriale, Maria Pesavento, Luigi Zeni. Plasmonic Chemical and Biological Sensors based on plastic optical fibers. Advanced Photonics. 2018; ():SeW1E.1.

Chicago/Turabian Style

Nunzio Cennamo; Sabato D'auria; Antonio Varriale; Maria Pesavento; Luigi Zeni. 2018. "Plasmonic Chemical and Biological Sensors based on plastic optical fibers." Advanced Photonics , no. : SeW1E.1.

Journal article
Published: 09 July 2016 in Analytical and Bioanalytical Chemistry
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Ephedrine is one of the main precursor compounds used in the illegal production of amphetamines and related drugs. Actually, conventional analytical methods such as high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), and gas chromatography-mass spectrometry (GC-MS) are used for the detection of ephedrine; sadly, these methods require qualified personnel and are time-consuming and expensive. In order to overcome these problems, in recent years, different methods have been developed based on the surface plasmon resonance (SPR) and electrochemical method. In this work, we present a simple, rapid, and effective method to detect the presence of ephedrine in solution, based on competitive fluorescence resonance energy transfer (FRET) assay. The antibody anti-ephedrine and ephedrine derivative were produced and labeled respectively, with two different fluorescent probes (donor and acceptor). The change in FRET signal intensity between donor and acceptor ephedrine compounds gives the possibility of detecting ephedrine traces of at least 0.81 ± 0.04 ppm (LOD). Graphical abstract A new Time-resolved Fluorescence Resonance Energy Transfer (FRET) assay for ephedrine detection.

ACS Style

Antonio Varriale; Vincenzo Manuel Marzullo; Stefano Di Giovanni; Andrea Scala; Alessandro Capo; Adelia Majoli; Angela Pennacchio; Maria Staiano; Sabato D’Auria. On the possibility of ephedrine detection: time-resolved fluorescence resonance energy transfer (FRET)-based approach. Analytical and Bioanalytical Chemistry 2016, 408, 6329 -6336.

AMA Style

Antonio Varriale, Vincenzo Manuel Marzullo, Stefano Di Giovanni, Andrea Scala, Alessandro Capo, Adelia Majoli, Angela Pennacchio, Maria Staiano, Sabato D’Auria. On the possibility of ephedrine detection: time-resolved fluorescence resonance energy transfer (FRET)-based approach. Analytical and Bioanalytical Chemistry. 2016; 408 (23):6329-6336.

Chicago/Turabian Style

Antonio Varriale; Vincenzo Manuel Marzullo; Stefano Di Giovanni; Andrea Scala; Alessandro Capo; Adelia Majoli; Angela Pennacchio; Maria Staiano; Sabato D’Auria. 2016. "On the possibility of ephedrine detection: time-resolved fluorescence resonance energy transfer (FRET)-based approach." Analytical and Bioanalytical Chemistry 408, no. 23: 6329-6336.