The present study concerns the Phoenician-Punic site of Motya, a small island set in Western Sicily (Italy), in the Marsala Lagoon (Stagnone di Marsala), between Trapani and Marsala. A big disposal pit, datable to between the first half of the 8th and the mid-6th centurybc, was identified in Area D. This context was sampled for plant macro-remains through bucket flotation. Palynological treatment and analysis were also performed on soil samples collected from each of the identified filling layers. The combination of the study of macro- and micro-remains has shown to be effective in answering questions concerning introduced food plants and agricultural practices, and native plants, including timber use. Here we investigate if a waste context can provide information about Phoenicians at Motya and their impact on the local plant communities. We found that human diet included cereals (mostly naked wheat), pulses and fruits. A focus was placed on weeds (includingLolium temulentumandPhalarisspp.) referable to different stages of crop processing. This aspect was enriched by the finding of cereal pollen, which suggests that threshing (if not even cultivation) was carried out on site. Palynology also indicates an open environment, with little to no forest cover, characterized by complex anthropogenic activities. Anthracology suggests the presence of typical Mediterranean plant taxa, including not only the shrubsPistacia lentiscusandErica multiflora, but also evergreen oaks. The presence of a stone pine nut and ofPinus pinea/pinasterin the pollen rain is noteworthy, suggesting the local occurrence of these Mediterranean pines outside their native distribution range. This represents the first such find in the central Mediterranean. Finally, the present study allows us to compare Motya’s past environment with the present one. The disappearance ofJuniperussp. andErica arboreafrom the present-day surroundings of the Marsala lagoon appears to be related to land-overexploitation, aridification or a combination of both processes.

Light microscopy is the conventional method used to investigate wood anatomy, identify the wood taxon, and assess its conservation state. It generally requires the mechanical cut of thin sections from a sample to obtain informative images. When dealing with wooden artworks or ancient remains (e.g., archaeological waterlogged wood), it is important to avoid sample destruction. In this work the efficacy of micro-magnetic resonance imaging (μ-MRI) to investigate the anatomy of waterlogged wood is assessed in comparison with light microscopy. Images along the three anatomical directions (transverse, tangential and radial) of six modern wood species and one archaeological specimen of waterlogged wood (from the Neolithic site "La Marmotta") were obtained both by μ-MRI and light microscopy. μ-MRI images were acquired virtually selecting 2D slices along the three wood anatomical directions. A 3D reconstruction was derived from 2D μ-MRI images. Conventional light microscopy histology was obtained by manually cutting thin sections. To the best of our knowledge, this is the first study in which high-resolution MR images and light microscopy images of the three anatomical directions of seven wood species are compared. The non-destructive μ-MRI approach allows to investigate the 2D and 3D topological organization of the whole waterlogged wood sample up to a resolution of 8 μm. Although the optical microscope attains higher image resolutions and remains superior in the observation of wood diagnostic characters, multi-parametric μ-MRI provides physiological investigation complementary to light microscopy, giving information concerning both a single section and the whole volume of the sample. The presented study may represent a starting point for further improvements of μ-MRI techniques applied to the non-destructive investigation of waterlogged wood samples, especially those of interest for cultural heritage.

This paper presents the first systematic results of integrated plant macrofossil and pollen analyses from the Horti Lamiani (1st-3rd century ce), an aristocratic residence with a luxury garden which was established on the Esquilino (Esquiline Hill) in Rome during the time of the first Emperor, Caesar Augustus (27 bce-14 ce) and later became one of the most famous Imperial gardens around the ancient city. Different types of plant remains such as charcoal, seeds and fruits and pollen were recovered from pits and pots in the garden and reflect the presence of plants there. There seem to have been ornamental shrubs which were probably grown as decorative modelled hedges and/or isolated bushes, as well as cultivated trees. Several ornamental flowering plants grew in pots. Although this archaeobotanical assemblage could represent some patches of wild vegetation still growing in the study area at the time of the Imperial garden, it is likely that most of the identified plants were intentionally planted and organised to create a glimpse of the past wild landscape of Rome. They have special characteristics, such as bearing coloured flowers or fruits and offering shade, that made them suitable for embellishing a magnificent garden. Moreover this reflects the Roman desire to control nature, testified by the expansion of luxury gardens during the Imperial period and the spread of decorative horticultural techniques, like the miniaturisation of trees and shaping of trees and shrubs by topiary.

The identification of the dyes present on a linen fragment from the tomb of Pharaoh Tutankhamun is the objective of the present study. Fiber optic reflectance spectroscopy (FORS) was applied to the archaeological sample for preliminary identification of the dyes and to better choose the extraction methodology for different areas of the sample. The innovative gel-supported micro-extraction with agar gel and the Nanorestore Gel^® High Water Retention (HWR) gel were applied to the archaeological sample after testing of the best concentration for the extraction of the agar gels substrates, performed on laboratory mock-ups by means of UV–Vis transmittance spectroscopy. Immediately after extraction, Ag colloidal pastes were applied on the gel surface and Surface Enhanced Raman Scattering (SERS) analysis was performed directly on them. The combination of information deriving from FORS and SERS spectra resulted in the successful identification of both indigo and madder and, in hypothesis, of their degradation products.

Archaeobotanical remains from the Phoenician – Punic site of Motya, set in the Marsala Lagoon in Western Sicily (Italy), were collected through flotation and sieving during the excavation campaigns of 2017–2019. Analyses focused on a sacrificial favissa, on the SW side of the Temple of Cappiddazzu, dedicated to Melqart/Herakles, where the buried remains of seven bovines were also found. Plant remains, preserved mostly by mineralisation, provide information about ritual practices. The retrieval of toxic plants to livestock (some Boraginaceae and Euphorbiaceae, and Anagallis arvensis) suggests their use to stun animals before sacrificing them. Additionally, remains referable to fruit (Vitis vinifera) and flower offerings (Verbena officinalis), as well as ornamental (Cupressus cf. sempervirens) and officinal plants (Borago officinalis) were also found.