Dr. José Luiz Da Silva

A reversed-phase liquid chromatographic separation with pulsed amperometric detection of phenolic acids at a glassy carbon electrode is described. Chromatographic separation was carried out in isocratic conditions using 0.20 mol·L⁻¹acetic acid (pH 5.0)/water (80 : 20,v/v) as mobile phase under constant working potential mode of 0.80 V. Chromatographic peaks presented high resolution and separation. Calibration curves exhibited excellent correlation coefficients, above 0.995. Linear ranges of the analytes, in mg L⁻¹, were of 0.018–18 (gallic acid), 0.146–19 (vanillic acid), 0.13–17 (caffeic acid), 0.016–16 (ferulic acid), and 0.008–17 (p-coumaric acid), respectively. Limits of detection ranged from 1.6 to 97 μg·L⁻¹and precision varied in 1.73–3.78% interval. Concentrations of 19 ± 0.51 mg·L⁻¹and 7.8 ± 2.5 mg·L⁻¹were found for vanillic and caffeic acids, respectively, in a sugarcane vinasse sample. Gallic, ferulic, and p-coumaric acids were not detected. Recovery results demonstrated that the proposed method is accurate, and it can be used to detect and quantify phenolic acids in sugarcane vinasse without any influence of interferents.

About Me

PhD (2017) and MSc (2013) in Chemistry from São Paulo State University (UNESP, Brazil), and BSc in Chemistry from the State University of Mato Grosso do Sul (UEMS, Brazil). He has conducted postdoctoral research in renowned institutions, contributing to electrochemical and analytical chemistry. At the National University of Engineering (UNI, Peru, 2022–2023), he worked with Prof. Adolfo La Rosa-Toro Gómez on polymer electrolytes for lithium-ion batteries. At UNESP, he collaborated with Prof. Nelson Ramos Stradiotto (2019–2021) on molecularly imprinted gas sensors for CO₂ detection and with Prof. Maria Valnice B. Zanoni (2017–2018) on semiconductor-modified membranes for photoelectrocatalytic water treatment. Currently, he is a researcher at UNI and part-time lecturer at Universidad Peruana Cayetano Heredia (UPCH). His research interests include electrochemical sensors (voltammetric and amperometric), nanomaterial-modified electrodes (metal nanoparticles, graphene, carbon nanofibres), voltamperometric techniques, HPLC, ion chromatography, electrochemical detection, electroanalytical method development, environmental analytical chemistry, novel materials, and lithium-ion batteries.

Dr. José Luiz Da Silva

Dr. José Luiz Da Silva

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