Dugbe orthonairovirus (DUGV) and Crimean-Congo hemorrhagic fever orthonairovirus (CCHFV) are tick-borne arboviruses within the order Bunyavirales. Both viruses are endemic in several African countries and can induce mild (DUGV, BSL 3) or fatal (CCHFV, BSL 4) disease in humans. Ruminants play a major role in their natural transmission cycle. Therefore, they are considered as suitable indicator animals for serological monitoring studies to assess the risk for human infections. Although both viruses do not actually belong to the same serogroup, cross-reactivities have already been reported earlier—hence, the correct serological discrimination of DUGV and CCHFV antibodies is crucial. In this study, 300 Nigerian cattle sera (150 CCHFV seropositive and seronegative samples, respectively) were screened for DUGV antibodies via N protein-based ELISA, indirect immunofluorescence (iIFA) and neutralization assays. Whereas no correlation between the CCHFV antibody status and DUGV seroprevalence data could be demonstrated with a newly established DUGV ELISA, significant cross-reactivities were observed in an immunofluorescence assay. Moreover, DUGV seropositive samples did also cross-react in a species-adapted commercial CCHFV iIFA. Therefore, ELISAs seem to be able to reliably differentiate between DUGV and CCHFV antibodies and should preferentially be used for monitoring studies. Positive iIFA results should always be confirmed by ELISAs.

Dugbe orthonairovirus (DUGV), a tick-borne zoonotic arbovirus, was first isolated in 1964 in Nigeria. For over four decades, no active surveillance was conducted to monitor the spread and genetic variation of DUGV. This study detected and genetically characterized DUGV circulating in cattle and their infesting ticks (Amblyomma and Rhipicephalus (Boophilus)) in Kwara State, North-Central Nigeria. Blood and or ticks were collected from 1051 cattle at 31 sampling sites (abattoirs and farms) across 10 local government areas of the State. DUGV detection was carried out by RT-qPCR, and positive samples sequenced and phylogenetically analysed. A total of 11824 ticks, mostly A. variegatum (36.0%) and R. (B.) microplus (63.9%), were obtained with mean tick burden of 12 ticks/cattle. Thirty-four (32 A. variegatum and two R. (B.) microplus) of 4644 examined ticks were DUGV-positive, whereas all of the cattle sera tested negative for DUGV genome. Whole genome sequence (S, M and L segments) and phylogenetic analyses indicate that the positive samples shared up to 99.88% nucleotide identity with and clustered around the Nigerian DUGV prototype strain IbAr 1792. Hence, DUGV with high similarity to the previously characterised strain has been detected in Nigeria. To our knowledge, this is the first report of DUGV in North-Central Nigeria and the most recent information after its last surveillance in 1974.

Resistance to antimicrobials is growing faster than the discovery of new drugs. This problem has raised the global concern of a possible pandemic, as the treatment of infections in both humans and animals is becoming more difficult. This study aimed to understand the opinions of practising veterinarians on the factors responsible for increasing antibiotic resistance among animals in Nigeria. A cross-sectional study of veterinarians from 36 states (including the Federal capital territory) was conducted using a paper-based structured questionnaire survey. The questionnaire was made available to the 1,117 veterinarians who attended 2023 Nigerian Veterinary Medical Association (NVMA). A simple random selection was used to select 295 veterinarians as respondents. The data collected were coded and stored electronically in a Microsoft Excel database and anonymised for further analysis. Statistical analyses were conducted using Excel and SPSS Statistics v24.0. Most veterinarians (89.8%) agreed that the antibiogram test (AST) is important before prescription; however, only a few (4.4%) request for an antibiogram before prescriptions to clients due to the unaffordability of the test by clients (x̄=3.21), the desire for rapid results (x̄=3.06), and the lack of diagnostic laboratories nearby (x̄=2.96). As a result, 61.5% reported that clients had complained that antibiotics prescribed did not work (curative). In addition, veterinarians opined that irrational antibiotic use among animal owners (x̄=4.34), an increased number of quackery (x̄=4.10) and ease of access of the public to antibiotics at veterinary pharmacies/shops (x̄=3.86) were the leading causes of increasing antimicrobial resistance in Nigeria. The significant factors responsible for veterinarians’ opinions were education (β = 0.929) and the value of conducting an antibiogram before prescription (β = 1.618) at the p ≤ 0.05 level of significance. This study revealed that irrational antibiotic use among animal owners, an increasing number of quackery and ease of access of the public to antibiotics at veterinary pharmacies/shops were the leading causes of increasing animal antimicrobial resistance in Nigeria. Therefore, this study suggests the development and implementation of policies and frameworks that promote antimicrobial stewardship and the establishment of veterinary laboratories in various communities in the country. Continuous education for veterinarians and livestock workers, especially on AMR, is also critical.

Rearing of dogs and other pets has become increasingly popular in modern society. Bacterial flora resides within the nasal and oral cavities of dogs and when chanced, can be pathogenic. Certain similarities between humans and dogs portends dangerous behavioral habits that could lead to zoonotic disease transmission. This study was aimed at isolation, identification and antibiotic profiling of bacteria from nasal swabs of apparently healthy dogs. The zoonotic risk was also considered. A total of 173 nasal swabs were collected from 173 apparently healthy dogs. Structured questionnaires were administered to investigate human behavioral habits. Two hundred and twenty two (222) bacterial isolates were obtained from the culture with ten (10) potentially pathogenic bacteria in the order of Escherichia coli (18.5%), Proteus species (17.1%), Staphylococcus aureus (14.0%), Klebsiella species (9.0%), Acinetobacter species (9.0%), coagulase negative Staphylococcus species (7.7%), Pseudomonas species (6.8%), Actinobacter species (6.8%), Citrobacter species (5.9%) and Streptococcus species (5.4%). Overall, the Gram negative isolates showed resistance to ciprofloxacin (9.3%), sparfloxacin (16.0%), perfloxacin (17.3%), ofloxacin (21.6%), chloramphenicol (34..6%), gentamycin (36.4%), streptomycin (37.%), septrin (49.4%), amoxillin (59.3%), augmentin (62.3%) while the Gram positive bacteria showed resistance to ciprofloxacin (3.3%), perfloxacin (6.7%), erythromycin (13.3%), streptomycin (21.7%), rocephin (28.3%), septrin (28.3%), gentamycin (36.7%), zinnacef (68.3%), ampiclox (81.7%) and amoxillin (85.0%). Multi-drug resistance (MDR) to three or more antimicrobials was observed in some of the isolates. Seventy - seven resistance patterns were observed, 16 in Gram positive and 61 in Gram negative bacteria. This study revealed MDR to two or more antimicrobials in all the isolates. These can pose antibiotic resistance challenges in situation of primary or secondary canine respiratory infections. Also, this study revealed that 82% of the dog owners/lovers had less than 50cm face-to-face contact with these dogs while playing with them, thus increasing their chances of acquiring MDR bacteria from apparently healthy dogs.

This study aimed to assess available assay methods for infectious bursal disease (IBD) diagnosis and seromonitoring in local birds