Introduction: Heart Rate Variability (HRV) and Pulse Rate Variability (PRV), are non-invasive techniques for monitoring changes in the cardiac cycle. Both techniques have been used for assessing the autonomic activity. Although highly correlated in healthy subjects, differences in HRV and PRV have been observed under various physiological conditions. The reasons for their disparities in assessing the degree of autonomic activity remains unknown. Methods: To investigate the differences between HRV and PRV, a whole-body cold exposure (CE) study was conducted on 20 healthy volunteers (11 male and 9 female, 30.3 ± 10.4 years old), where PRV indices were measured from red photoplethysmography signals acquired from central (ear canal, ear lobe) and peripheral sites (finger and toe), and HRV indices from the ECG signal. PRV and HRV indices were used to assess the effects of CE upon the autonomic control in peripheral and core vasculature, and on the relationship between HRV and PRV. The hypotheses underlying the experiment were that PRV from central vasculature is less affected by CE than PRV from the peripheries, and that PRV from peripheral and central vasculature differ with HRV to a different extent, especially during CE. Results: Most of the PRV time-domain and Poincaré plot indices increased during cold exposure. Frequency-domain parameters also showed differences except for relative-power frequency-domain parameters, which remained unchanged. HRV-derived parameters showed a similar behavior but were less affected than PRV. When PRV and HRV parameters were compared, time-domain, absolute-power frequency-domain, and non-linear indices showed differences among stages from most of the locations. Bland-Altman analysis showed that the relationship between HRV and PRV was affected by CE, and that it recovered faster in the core vasculature after CE. Conclusion: PRV responds to cold exposure differently to HRV, especially in peripheral sites such as the finger and the toe, and may have different information not available in HRV due to its non-localized nature. Hence, multi-site PRV shows promise for assessing the autonomic activity on different body locations and under different circumstances, which could allow for further understanding of the localized responses of the autonomic nervous system.

The photoplethysmogram (PPG) signal is widely used in pulse oximeters and smartwatches. A fundamental step in analysing the PPG is the detection of heartbeats. Several PPG beat detection algorithms have been proposed, although it is not clear which performs best. Objective: This study aimed to: (i) develop a framework with which to design and test PPG beat detectors; (ii) assess the performance of PPG beat detectors in different use cases; and (iii) investigate how their performance is affected by patient demographics and physiology. Approach: Fifteen beat detectors were assessed against electrocardiogram-derived heartbeats using data from eight datasets. Performance was assessed using the F ₁ score, which combines sensitivity and positive predictive value. Main results: Eight beat detectors performed well in the absence of movement with F ₁ scores of ≥90% on hospital data and wearable data collected at rest. Their performance was poorer during exercise with F ₁ scores of 55%–91%; poorer in neonates than adults with F ₁ scores of 84%–96% in neonates compared to 98%–99% in adults; and poorer in atrial fibrillation (AF) with F ₁ scores of 92%–97% in AF compared to 99%–100% in normal sinus rhythm. Significance: Two PPG beat detectors denoted ‘MSPTD’ and ‘qppg’ performed best, with complementary performance characteristics. This evidence can be used to inform the choice of PPG beat detector algorithm. The algorithms, datasets, and assessment framework are freely available.

Photoplethysmography (PPG) is a photometric technique used for the measurement of volumetric changes in the blood. The recent interest in new applications of PPG has invigorated more fundamental research regarding the origin of the PPG waveform, which since its discovery in 1937, remains inconclusive. A handful of studies in the recent past have explored various hypotheses for the origin of PPG. These studies relate PPG to mechanical movement, red blood cell orientation or blood volume variations. Objective : Recognising the significance and need to corroborate a theory behind PPG formation, the present work rigorously investigates the origin of PPG based on a realistic model of light–tissue interactions. Approach : A three-dimensional comprehensive Monte Carlo model of finger-PPG was developed and explored to quantify the optical entities pertinent to PPG (e.g. absorbance, reflectance, and penetration depth) as the functions of multiple wavelengths and source-detector separations. Complementary to the simulations, a pilot in vivo investigation was conducted on eight healthy volunteers. PPG signals were recorded using a custom-made multiwavelength sensor with an adjustable source-detector separation. Main results : Simulated results illustrate the distribution of photon–tissue interactions in the reflectance PPG geometry. The depth-selective analysis quantifies the contributions of the dermal and subdermal tissue layers in the PPG wave formation. A strong negative correlation (r = −0.96) is found between the ratios of the simulated absorbances and measured PPG amplitudes. Significance : This work quantified for the first time the contributions of different tissue layers and sublayers in the formation of the PPG signal.

For the last two decades, pulse oximetry has been used as a standard procedure for monitoring arterial oxygen saturation (SpO2). However, SpO2 measurements made from extremities such as the finger, ear lobe and toes become susceptible to inaccuracies when peripheral perfusion is compromised. To overcome these limitations, the external auditory canal has been proposed as an alternative monitoring site for estimating SpO2, on the hypothesis that this central site will be better perfused. Therefore, a dual wavelength optoelectronic probe along with a processing system was developed to investigate the suitability of measuring photoplethysmographic (PPG) signals and SpO2 in the human auditory canal. A pilot study was carried out in 15 healthy volunteers to validate the feasibility of measuring PPGs and SpO2 from the ear canal (EC), and comparative studies were performed by acquiring the same signals from the left index finger (LIF) and the right index finger (RIF) in conditions of induced peripheral vasoconstriction (right hand immersion in ice water). Good quality baseline PPG signals with high signal-to-noise ratio were obtained from the EC, the LIF and the RIF sensors. During the ice water immersion, significant differences in the amplitude of the red and infrared PPG signals were observed from the RIF and the LIF sensors. The average drop in amplitude of red and infrared PPG signals from the RIF was 52.7% and 58.3%. Similarly, the LIF PPG signal amplitudes have reduced by 47.52% and 46.8% respectively. In contrast, no significant changes were seen in the red and infrared EC PPG amplitude measurements, which changed by +2.5% and -1.2% respectively. The RIF and LIF pulse oximeters have failed to estimate accurate SpO2 in seven and four volunteers respectively, while the EC pulse oximeter has only failed in one volunteer. These results suggest that the EC may be a suitable site for reliable monitoring of PPGs and SpO2s even in the presence of peripheral vasoconstriction.

Pulse oximeters rely on the technique of photoplethysmography (PPG) to estimate arterial oxygen saturation (SpO\(_2\)). In conditions of poor peripheral perfusion such as hypotension, hypothermia, and vasoconstriction, the PPG signals detected are often weak and noisy, or in some cases unobtainable. Hence, pulse oximeters produce erroneous SpO\(_2\) readings in these circumstances. The problem arises as most commercial pulse oximeter probes are designed to be attached to peripheral sites such as the finger or toe, which are easily affected by vasoconstriction. In order to overcome this problem, the ear canal was investigated as an alternative site for measuring reliable SpO\(_2\) on the hypothesis that blood flow to this central site is preferentially preserved. A novel miniature ear canal PPG sensor was developed along with a state of the art PPG processing unit to investigate PPG measurements from the bottom surface of the ear canal. An in vivo study was carried out in 15 healthy volunteers to validate the developed technology. In this comparative study, red and infrared PPGs were acquired from the ear canal and the finger of the volunteers, whilst they were undergoing artificially induced hypothermia by means of cold exposure (10 \(^\circ\)C). Normalised Pulse Amplitude (NPA) and SpO\(_2\) was calculated from the PPG signals acquired from the ear canal and the finger. Good quality baseline PPG signals with high signal-to-noise ratio were obtained from both the PPG sensors. During cold exposure, significant differences were observed in the NPA of the finger PPGs. The mean NPA of the red and infrared PPGs from the finger have dropped by >80%. Contrary to the finger, the mean NPA of red and infrared ear canal PPGs had dropped only by 0.2 and 13% respectively. The SpO\(_2\)s estimated from the finger sensor have dropped below 90% in five volunteers (failure) by the end of the cold exposure. The ear canal sensor, on the other hand, had only failed in one volunteer. These results strongly suggest that the ear canal may be used as a suitable alternative site for monitoring PPGs and arterial blood oxygen saturation at times were peripheral perfusion is compromised.

In this paper, we present the design, development, and validation of a `modular photoplethysmography (PPG) system called ZenPPG. This portable, dual-channel system has the capability to produce ``raw'' PPG signals at two different wavelengths using commercial and/or custom-made PPG sensors. The system consists of five modules, each consisting of circuitry required to perform specific tasks, and are all interconnected by a system bus. The ZenPPG system also facilitates the acquisition of other physiological signals on-demand including electrocardiogram (ECG), respiration, and temperature signals. This report describes the technical details and the evaluation of the ZenPPG along with results from a pilot in vivo study on healthy volunteers. The results from the technical evaluations demonstrate the superiority and flexibility of the system. Also, the systems' compatibility with commercial pulse oximetry sensors such as the Masimo reusable sensors was demonstrated, where good quality raw PPG signals were recorded with the signal-to-noise ratio (SNR) of 50.65 dB. The estimated arterial oxygen saturation (SpO₂) values from the system were also in close agreement with commercial pulse oximeters, although the accuracy of the reported SpO₂ value is dependent on the calibration function used. Future work is targeted toward the development of variations of each module, including the laser driver and fiber optic module, onboard data acquisition and signal processing modules. The availability of this system will help researchers from a wide range of disciplines to customize and integrate the ZenPPG system to their research needs and will most definitely enhance research in related fields.