Khathutshelo Agree Nephawe

Prof. Khathutshelo Agree Nephawe

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Research Focus & Metrics

Academic Career Length

11Years (2016-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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31 March 2025 in Frontiers in Animal Science
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Journal Article
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Peter Ayodeji IdowuPeter Ayodeji Idowu
Peter Ayodeji Idowu
Takalani MpofuTakalani Mpofu
Takalani Mpofu
AMAletta Matshidiso Magoro
Aletta Matshidiso Magoro
MMMamokoma C Modiba
Mamokoma C Modiba
Khathutshelo Agree NephaweKhathutshelo Agree Nephawe
Khathutshelo Agree Nephawe
BMBohani Mtileni
Bohani Mtileni
The poultry industry is continuously seeking strategies to improve chicken health, welfare, and productivity while minimizing the use of antibiotics. Probiotics, as a natural alternative, have gained considerable attention due to their ability to modulate the gut microbiota, enhance immune function, and improve productive performance. The aim of this article is to provide updated information on the importance of probiotics in chicken. To achieve this, a systematic review was conducted to synthesize current findings on the impact of probiotics on chicken gut microbiota composition, immune responses, behavior, productive traits, and meat quality using literature databases such as PubMed, CABI Abstract, Google Scholar, and ScienceDirect from April 2010. The PRISMA method was adopted, where 85 articles met the criteria for this review article after several exclusion criteria. The review stated that due to the influence of the intestinal microbial balance, probiotics promote beneficial bacterial populations, suppress pathogens, improve gut health, and enhance nutrient absorption, improving growth performance. Additionally, the immunomodulatory effects of probiotics help strengthen the chicken’s immune system, reducing disease susceptibility. Moreover, recent studies suggest that probiotics may positively influence chicken behavior, particularly by reducing stress, enhancing overall health, and improving welfare conditions. This review also addresses gaps in knowledge, highlighting areas where further research is needed to optimize probiotic use in poultry production systems. Understanding both the short- and long-term effects of probiotics on chicken health and performance will provide critical insights for developing sustainable strategies to boost poultry industry outcomes.

17 June 2024 in Sustainability
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Journal Article
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Ephodia SihlanguEphodia Sihlangu
Ephodia Sihlangu
DLDibungi Luseba
Dibungi Luseba
TRThierry Jean Claude Regnier
Thierry Jean Claude Regnier
PMPrimrose Magama
Primrose Magama
ICIdan Chiyanzu
Idan Chiyanzu
Khathutshelo Agree NephaweKhathutshelo Agree Nephawe
Khathutshelo Agree Nephawe
The agricultural industry produces a substantial quantity of organic waste, and finding a suitable method for disposing of this highly biodegradable solid waste is a difficult task. The utilisation of anaerobic digestion for agricultural waste is a viable technological solution for both renewable energy production (biogas) and waste treatment. The primary objective of the study was to assess the composition of biogas, namely the percentages of methane, carbon dioxide, ammonia, and hydrogen sulphide. Additionally, the study aimed to quantify the amount of biogas produced and determine the methane yield (measured in NmL/g VS) from different agricultural substrates. The biochemical methane potential (BMP) measurements were conducted in triplicate using the BPC Instruments AMPTS II instrument. The substrates utilised in the investigation were chosen based on their accessibility. The substrates used in this study comprise cattle manure, chicken manure, pig manure, tomato plants, tomatoes, cabbage, mixed fruits, mixed vegetables, dog food, and a co-digestion of mixed vegetables, fruits, and dog food (MVMFDF). Prior to the cleaning process, the makeup of the biogas was assessed using the BIOGAS 5000, a Geotech Analyser. The AMPTS II flow cell automatically monitored and recorded the volume of bio-methane produced after the cleaning stage. The data were examined using the Minitab-17 software. The co-digestion of mixed vegetables, mixed fruits, and dog food (MVMFDF) resulted in the highest methane level of 77.4%, followed by mixed fruits at 76.6%, pig manure at 72.57%, and mixed vegetables at 70.1%. The chicken manure exhibited the greatest levels of ammonia (98.0 ppm) and hydrogen sulphide (589 ppm). Chicken manure had the highest hydrogen sulphide level, followed by pig manure (540 ppm), tomato plants (485 ppm), mixed fruits (250 ppm), and MVMFDF (208 ppm). Ultimately, the makeup of biogas is greatly affected by the unique qualities of each substrate. Substrates containing elevated quantities of hydrogen sulphide, such as chicken manure, require the process of biogas scrubbing. This is because they contain substantial amounts of ammonia and hydrogen sulphide, which can cause corrosion to the equipment in biogas plants. This emphasises the crucial need to meticulously choose substrates, with a specific focus on their organic composition and their capacity to generate elevated methane levels while minimising contaminants. Substrates with a high organic content, such as agricultural waste, are optimal for maximising the production of methane. Furthermore, the implementation of biogas scrubbing procedures is essential for efficiently decreasing carbon dioxide and hydrogen sulphide levels in biogas. By considering and tackling these problems, the effectiveness of biogas generation can be enhanced and its ecological consequences alleviated. This strategy facilitates the advancement of biogas as a sustainable energy source, hence contributing to the attainment of sustainable development goals (SDGs).

21 October 2022 in Veterinary World
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Journal Article
Open access
Takalani MpofuTakalani Mpofu
Takalani Mpofu
Khathutshelo Agree NephaweKhathutshelo Agree Nephawe
Khathutshelo Agree Nephawe
BMBohani Mtileni
Bohani Mtileni
Gastrointestinal parasitism, particularly nematode infection, is a major health issue affecting goats worldwide, resulting in clinical diseases and productivity loss. Prevalent gastrointestinal parasites (GIPs) affecting goats in South Africa are the Strongyloides papillosus, Eimeria spp., and Strongyles, especially the Haemonchus contortus and Trichostrongylus spp. According to the issues discussed in this paper and by other authors, the prevalence and intensity of various GIPs vary with an animal's location, breed, age, sex, and season. Because GIPs easily develop resistance to chemical treatment, selecting and breeding genetically GIP-resistant animals would be a relatively simple and inexpensive strategy for reducing or eliminating the current reliance on chemotherapy. Potential phenotypic indicators for selecting GIP-resistant goats include parasitological, immunological, and pathological phenotypic markers. Synergistic use of these indicators should be encouraged for a more accurate simplified genotype selection of resistant animals. Genes with Mendelian inheritance, particularly those involved in immunoregulatory mechanisms, have been identified in goats. Exploring this knowledge base to develop cost-effective molecular tools that facilitate enhanced genetic improvement programs is a current challenge. Future statistical and biological models should investigate genetic variations within genomic regions and different candidate genes involved in immunoregulatory mechanisms, as well as the identification of single nucleotide polymorphisms known to affect GIP infection levels.

20 January 2023 in Sustainability
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TRTumelo Francinah Ramukhithi
Tumelo Francinah Ramukhithi
Khathutshelo Agree NephaweKhathutshelo Agree Nephawe
Khathutshelo Agree Nephawe
Takalani MpofuTakalani Mpofu
Takalani Mpofu
TRThomas Raphulu
Thomas Raphulu
KMKaren Munhuweyi
Karen Munhuweyi
BMBohani Mtileni
Bohani Mtileni
An important factor in determining the success of a small-scale broiler farm is its economic viability and efficiency. During times of trouble for the industry, the idea receives more attention. The conceptual considerations of economic sustainability and efficiency are frequently quite constrained, according to the difficulties raised in this study and by other authors. There is a lack of information about South Africa’s small-scale broiler production’s economic viability and effectiveness. Furthermore, it is clear that small-scale broiler producers have the ability to increase their economic efficiency. By reducing the mortality rate, feed conversion rate, and production duration, both their technical and financial efficiency could be improved. Profitability in the production of broilers will be considerably increased by lowering the cost of these variable inputs, particularly feed and day-old chicks. Additionally, raising the education level, capacity utilization ratio, and broiler production would all contribute to raising the farms’ efficiency levels. To ensure effective resource use and to maximize practicable profit, small-scale broiler producers who are not operating close to the profit frontier must make efforts to reduce both technical and allocation inefficiencies. Collectively, all these measures would ensure the economic sustainability of small-scale farmers in South Africa would be met. Moreover, the sustainability of small-scale broiler producers can be achieved if strategies that build local capacity and that empower them to sustain high levels of productivity are provided. In addition, the efficient use of resources will ensure that productivity is enhanced, and might increase profitability. It is therefore important to ensure that small-scale broiler producers achieve maximum profit for a given set of inputs. Approaches in assessing the farm-level profitability such as cost-benefit and gross margin analyses can be used.

28 November 2021 in Animals
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JNJabulani Nkululeko Ngcobo
Jabulani Nkululeko Ngcobo
FRFhulufhelo Vincent Ramukhithi
Fhulufhelo Vincent Ramukhithi
Khathutshelo Agree NephaweKhathutshelo Agree Nephawe
Khathutshelo Agree Nephawe
Takalani MpofuTakalani Mpofu
Takalani Mpofu
TCTlou Caswell Chokoe
Tlou Caswell Chokoe
TNTshimangadzo Lucky Nedambale
Tshimangadzo Lucky Nedambale
The demand to conserve indigenous species through the cryo-gene bank is increasing. Spermatozoa remain sensitive to cryopreservation damages especially that of avian species thus limiting the use of reproductive biotechnologies such as artificial insemination in the conservation programs. Long-chain polyunsaturated fatty acid (LCPUFAs), specifically omega n-3, expanded a research interest to improve animal reproductive efficiency through improving spermatozoa quality. This is driven by the fact that mammals cannot synthesize omega-3 de-novo because they lack delta-12 and delta-15 desaturase enzymes thus supplemented in the diet is mandatory. Delta-12 and delta-15 add a double bond at the 12th and 15th carbon-carbon bond from the methyl end of fatty acids, lengthening the chain to 22 carbon molecules. Fish oil is a pioneer source of omega n-3 and n-6 fatty acids. However, there is a report that numerous fisheries are over-exploited and could collapse. Furthermore, processing techniques used for processing by-products could complement alterations of the amino acid profile and reduce protein retrieval. Alternatively, flaxseed oil contains ±52–58% of total fatty acids and lignans in the form of α-linolenic and linoleic acid. Alpha-linolenic acid (ALA,18:3n-3) is enzymatically broken-down de-novo by delta-6 desaturase and lengthened into a long-chain carbon molecule such as eicosapentaenoic acid (C20:5n-3). Nevertheless, controversial findings following the enrichment of diet with flaxseed oil have been reported. Therefore, this paper is aimed to postulate the role of flaxseed oil as an alternative source of omega n-3 and n-6 fatty acids to improve semen quality and quantity from livestock animals. These include the interaction between docosahexaenoic acid (DHA) and spermatogenesis, the interaction between docosahexaenoic acid (DHA) and testicular cells, and the effect of flaxseed oil on semen quality. It additionally assesses the antioxidants to balance the level of PUFAs in the semen.

25 June 2022 in Diversity
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Journal Article
Open access
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JNJabulani Nkululeko Ngcobo
Jabulani Nkululeko Ngcobo
TNTshimangadzo Lucky Nedambale
Tshimangadzo Lucky Nedambale
Khathutshelo Agree NephaweKhathutshelo Agree Nephawe
Khathutshelo Agree Nephawe
Takalani MpofuTakalani Mpofu
Takalani Mpofu
TCTlou Caswell Chokoe
Tlou Caswell Chokoe
FRFhulufhelo Vincent Ramukhithi
Fhulufhelo Vincent Ramukhithi
South African indigenous breeds’ population is decreasing at a time when their genetic material is mandatory due to the rising climate change and global warming. South African indigenous sheep breeds include Namaqua Afrikaner, Zulu, BaPedi, and Damara sheep. These breeds are the most preferred breeds by rural farmers in South Africa due to their adaptability, low feed, and veterinary requirements. However, since they are characterized by small body sizes, farmers tend to crossbreed them with exotic breeds. An early survey conducted in Kwa-Zulu Natal revealed a 7.5% decline in Zulu sheep between 2008–2011. It has recently been observed that the population left is genotypically mixed with exotic genetic material due to uncontrolled breeding techniques that rural farmers apply. Therefore, the aim of this review is to address the present status, difficulties, and conservation approaches applied to save these breeds. However, this review will be limited to the current extinction status as it appears in the Food and Agriculture Organization (FAO) system, data from recent studies, difficulties limiting the conservation success of these breeds, and the current conservation approaches in use to conserve these breeds.