Kontinuirana kontrola kvaliteta razređenog semena nerastova je jedan od najbitnijih preduslova za uspešnu svinjarsku proizvodnju. Ovakvim načinom rada se može uočiti pad kvaliteta razređenog semena, što može imati uticaj na koncepciju krmača, a sa druge strane, ovim analizama obezbeđuje se mogućnost za blagovremenu reakciju u cilju sprečavanja eventualnih problema. Analize koje se sprovode u laboratoriji za reprodukciju Naučnog instituta za veterinarstvo "Novi Sad" su CASA (kompjuterska analiza semena) i protočna citometrija (test statusa akrozoma i membrane spermatozoida; test strukture hromatina i DNK fragmentacije spermatozoida). Uz te analize, radi se i pregled supravitalno obojenog preparata po Bloom-u, modifikovano po Jovičinu (2012). Cilj ovog rada je prikaz kontinuirane kontrole kvaliteta razređenog semena u toku jedne godine, sa jedne komercijalne farme, u koji je uključeno 549 uzoraka razređenog semena od 124 nerasta. Uzorci su sortirani po kvartalima, gde su date prosečne vrednosti za svaki kvartal i za celokupnu godinu. Tokom prvog kvartala (januar-mart) kompjuterskom analizom semena je utvrđen procenat ukupno pokretnih spermatozoida koji je u proseku iznosio 75,39%, dok je procenat progresivno pokretnih spermatozoida u proseku iznosio 51,51%. Tokom drugog kvartala (april-jun) ovi procenti počinju blago da opadaju (ukupna i progresivna pokretljivost, 71,63% i 48,08%), a takav trend se nastavljao i tokom trećeg kvartala (jul-septembar) (ukupna i progresivna pokretljivost, 68,90% i 43,95%). U četvrtom kvartalu došlo je do poboljšanja kvaliteta istih parametara (ukupna i progresivna pokretljivost, 74,11% i 50,61%). U proseku za celu godinu, procenat ukupno pokretnih spermatozoida je iznosio 72,51%, dok je procenat progresivno pokretnih iznosio 48,54%. Testom strukture hromatina i DNK fragmentacije spermatozoida nije utvrđena velika oscilacija u procentu spermatozoida sa oštećenjem hromozoma (u proseku za celu godinu ovaj procenat je iznosio 6,09%). Pregledom supravitalno obojenih preparata razređeno seme je (prema internoj klasifikaciji laboratorije) u proseku svrstano u "II klasu" (50% živih intaktnih spermatozoida). Takođe, slično rezultatima dobijenim na kompjuterskoj analizi sperme, i ovom analizom je utvrđen slabiji kvalitet semena tokom trećeg kvartala. Procenat živih intaktnih spermatozoida od prvog do četvrtog kvartala je u proseku iznosio redom 51%, 52%, 44% i 53%. Analizom ovih rezultata zaključuje se da je u toku toplijih meseci došlo do pada kvaliteta razređenog semena. Mikroklimat u objektima gde borave nerastovi je od izuzetne važnosti da bi se omogućila maksimalna efikasna reproduktivna eksploatacija nerastova i u toplijem periodu godine u Republici Srbiji, kao i kontinuirana kontrola kvaliteta razređenog semena.

Rodents represent one of the most significant biotic hazards in food industry facilities. Their presence can cause direct contamination of food with feces, urine, and hair, as well as indirect contamination through the transmission of pathogenic microorganisms such as Salmonella spp., Listeria monocytogenes, and Leptospira spp. Modern food safety standards (HACCP, BRC, IFS, ISO 22000) require food businesses to establish a systematic pest control approach based on the principles of Integrated Pest Management (IPM). This review presents current scientific knowledge and practical achievements in the field of rodent control in the food industry.

Geographic Information Systems (GIS) represent a key tool in modern research in veterinary epidemiology, enabling precise integration, analysis, and visualization of spatial data related to the spread and control of infectious animal diseases. QGIS is a free and open-source software platform used for map creation, spatial analysis, and data processing relevant to the surveillance and control of infectious diseases in domestic animals. The application of QGIS technology enables the identification of spatial patterns in the spread of infectious disease agents, identification of high-risk areas, and the assessment of various environmental factors that may influence transmission pathways and the spread of infectious diseases. QGIS can be used for planning and implementing biosecurity measures in different production systems, as well as for preparing and conducting vaccination campaigns, leading to more effective surveillance, control, and prevention of infectious diseases in domestic animals. The study presents practical examples of QGIS applications in veterinary epidemiology, with a particular focus on the spatial spread analysis of African swine fever (ASF) in the Republic of Serbia. QGIS was used to perform spatial analysis of risk factors such as the number of pigs in various regions and the structure of farms. This approach enabled the identification of potential high-risk zones and improved understanding of population dynamics in ASF spread. The analysis was supported by statistical data processing, including visualization with pie charts integrated into spatial maps, providing a clearer overview of risk factors. This approach may contribute to more informed decision-making in the control and prevention of infectious diseases in domestic animals. Geospatial analyses can play a significant role in national plans for the control and prevention of infectious diseases in domestic animals. Therefore, it is essential that the use of such tools be actively considered as part of national programs for the control, prevention, and eradication of infectious animal diseases.

Extensive pig production represents a significant segment of livestock production in Serbia. Due to the continuous presence of African swine fever (ASF) in Serbia since 2019, with the predominant number of outbreaks diagnosed on this type of holdings, the level of biosecurity measures applied on them is of particular importance. However, the impact of sociodemographic and structural characteristics of holdings on the level of applied biosecurity measures has not been sufficiently studied. The aim of this study was to assess the level of biosecurity measures on extensive pig holdings in Vojvodina using the Biocheck.UGent tool, and to examine the relationship between biosecurity level and farmer experience and holding size. A cross-sectional study was conducted in 2026 and included 26 rural holdings and small extensive-type farms in 12 municipalities within 4 districts in Vojvodina. A total of 3,592 pigs were recorded, with an average of 138 animals per holding (range 12-600). The average working experience of farmers in pig production was 25.5 years (range 5-50). Biosecurity was evaluated using the Biocheck.UGent questionnaire. Pearson correlation analysis was applied to examine the relationship between biosecurity scores, number of animals, and years of farmer experience. The mean overall biosecurity score was 65.5% (range 35-83%), with external biosecurity (64.0%) being lower than internal biosecurity (68.8%). Correlation analysis showed no statistically significant relationship between years of farmer experience and overall biosecurity score (r=0.078; p>0,05), nor between experience and external (r=0.158; p>0,05) or internal biosecurity (r=-0.078; p>0,05). Similarly, no significant correlation was found between the number of animals per holding and overall biosecurity score (r=-0.137; p>0,05). The results indicate that neither long-term farmer experience nor holding size significantly determine the level of biosecurity. This finding suggests that empirical experience without structured education is insufficient for adequate implementation of biosecurity measures. Implementation of educational and advisory programs represents a necessary prerequisite for improving biosecurity on extensive-type holdings in Vojvodina.

Antimicrobial resistance in avian pathogenic Escherichia coli (APEC) poses a significant challenge to poultry production, highlighting the need for effective alternatives to conventional antimicrobials. This study aimed to evaluate the antibacterial activity of selected essential oils (EOs) against multidrug-resistant APEC isolates and assess the in vivo effects of an oregano–thyme EO-based formulation in experimentally infected broilers. Six EOs were screened in vitro using the broth microdilution method, and oregano and thyme EOs, showing the strongest activity (MIC 0.003% and 0.003–0.007%, respectively), were further evaluated in two combinations. The more active combination was formulated for administration via drinking water and tested in 420 Ross 308 broilers experimentally challenged with APEC. Birds were allocated to untreated control, infected untreated, EO-treated, enrofloxacin-treated, and enrofloxacin + reduced-dose EO formulation groups. Mortality did not differ significantly among groups. A binomial GLMM identified significant differences in faecal consistency among experimental groups (likelihood-ratio χ² = 32.90, df = 4, p < 0.001), while no significant differences were observed in body weight, feed intake, or overall FCR. Significant differences were detected in AST, glucose, total bilirubin, and calcium concentrations. Overall, the EO-based formulation demonstrated antibacterial activity in vitro and was associated with changes in intestinal status following APEC challenge, while no consistent improvement in productive performance was observed. These findings support further investigation of EO-based formulations as potential non-antibiotic approaches for managing APEC-associated intestinal disturbances in broiler chickens.