The impact of donor age on Adipose-derived mesenchymal stem cell (ASC) functionality and safety remains insufficiently characterized, particularly in equine models. This study investigates the influence of age on ASCs proliferation dynamics and the expression of tumorigenic and apoptosis-related markers. Equine ASCs were isolated from juvenile (15 years) horses and assayed across multiple passages. The relative mRNA expressions of pluripotency (Oct4), tumorigenic (CA9), and apoptosis-related (Bax and Bcl 2) markers were evaluated. The Gompertz growth model, population doubling time (PDT), and tissue non-specific ALP activity also followed. The expression of pluripotency and tumorigenic markers showed passage-dependent up-regulation, raising concerns about prolonged culture expansion. Apoptotic regulation displayed a shift with aging, as evidenced by alterations in the Bax/Bcl2 ratio, suggesting compromised cell survival in older ASCs. An age-associated decline in proliferation rates was established, as evidenced by declining alkaline phosphatase (ALP) activity. These findings underscore the necessity for stringent age-based selection criteria in equine stem cell therapies and the challenges associated with using autologous stem cells for regenerative therapies in aged horses. Future research should focus on molecular interventions to mitigate age-related functional decline, ensuring the safety and efficacy of ASCs-based regenerative medicine in equine practice.

In this paper, three discrete transforms related to vector spaces over finite fields are studied. For our purposes, and according to the properties of the finite fields, the most suitable transforms are as follows: for binary fields, this is the Walsh–Hadamard transform; for odd prime fields, the Vilenkin–Chrestenson transform; and for composite fields, the trace transform. A factorization of the transform matrices using Kronecker power is given so that the considered discrete transforms are reduced to the fast discrete transforms. Examples and applications are also presented of the considered transforms in coding theory for calculating the weight distribution of a linear code.

The paper presents an information platform for working with cardiac data registered with ECG, PPG, Holter device. The platform enables input, editing, processing and storage of the registered data and the results of the analyzes carried out on them. The report presents the options for creating an archive of the cardiology database.