The development of social production and the agglomeration of the urban population have brought tremendous pressure to transportation infrastructure. However, the impacts of transportation development on urban land use systems have not been well investigated. Under the pressure of limited land resources, the impact of transportation infrastructure on urban land use efficiency (ULUE) is receiving increasing attention from scholars and needs to be explored. By collecting panel data from 30 regions in China from 2003 to 2018, in this study we constructed a spatial Durbin model and a panel threshold regression model to explore the spatial spillover effects and threshold effects of transportation infrastructure on ULUE. The most obvious findings emerging from this study are that (1) ULUE is not randomly distributed over different regions in China, but has an obvious positive spatial correlation; (2) transportation infrastructure has significant positive direct and spatial spillover effects on ULUE and the direct effects of transportation infrastructure (0.823) are significantly stronger than the spatial spillover effects (0.263); (3) the impact of transportation infrastructure on ULUE has a significant double threshold effect, and the threshold values are 4.520 and 6.429 respectively, and with the improvement of transportation infrastructure, its marginal effects on ULUE show a downward trend. This paper provides theoretical support for policymakers to achieve cross-regional cooperation on land use and transportation infrastructure construction and inspirations for sustainable development.