Humanitas University

Mario Valenti; Luigi Gargiulo; Luciano Ibba; Francesco Toso; Antonio Costanzo; Alexia Bertuzzi; Sofia Manara; Alessandra Narcisi. Apremilast for the treatment of pustular psoriasis induced by neoadjuvant ifosfamide plus etoposide chemotherapy for Ewing Sarcoma: a case report. 2024, 35, 2319303 .

Mario Valenti, Luigi Gargiulo, Luciano Ibba, Francesco Toso, Antonio Costanzo, Alexia Bertuzzi, Sofia Manara, Alessandra Narcisi. Apremilast for the treatment of pustular psoriasis induced by neoadjuvant ifosfamide plus etoposide chemotherapy for Ewing Sarcoma: a case report. . 2024; 35 (1):2319303.

Mario Valenti; Luigi Gargiulo; Luciano Ibba; Francesco Toso; Antonio Costanzo; Alexia Bertuzzi; Sofia Manara; Alessandra Narcisi. 2024. "Apremilast for the treatment of pustular psoriasis induced by neoadjuvant ifosfamide plus etoposide chemotherapy for Ewing Sarcoma: a case report." 35, no. 1: 2319303.

Introduction: Degenerative meniscal tears are the most common meniscal lesions, representing huge clinical and socio-economic burdens. Their role in knee osteoarthritis (OA) onset and progression is well established and demonstrated by several retrospective studies. Effective preventive measures and non-surgical treatments for degenerative meniscal lesions are still lacking, also because of the lack of specific and accurate animal models in which test them. Thus, we aim to develop and validate an accurate animal model of meniscus degeneration.Method: Three different surgical techniques to induce medial meniscus degenerative changes in ovine model were performed and compared. A total of 32 sheep (stifle joints) were subjected to either one of the following surgical procedures: a) direct arthroscopic mechanical meniscal injury; b) peripheral devascularization and denervation of medial meniscus; c) full thickness medial femoral condyle cartilage lesion. In all the 3 groups, the contralateral joint served as a control.Result: From a visual examination of the knee joint emerged a clear difference between control and operated groups, in the menisci but also in the cartilage, indicating the onset of OA-related cartilage degeneration. The meniscal and cartilaginous lesions were characterized by different severity and location in the different groups. For instance, a direct meniscal injury caused cartilaginous lesions especially in the medial part of the condyles, and the other approaches presented specific signature. Evaluation of scoring scales (e.g. ICRS score) allowed the quantification of the damage and the identification of differences among the four groups.Conclusion: We were effectively able to develop and validate a sheep model of meniscal degeneration which led to the onset of OA. This innovative model will allow to test in a pre-clinical relevant setting innovative approaches to prevent meniscal-related OA.Funding: Project PNRR-MAD-2022-12375978 funded by Italian Ministry of Health

D. D’aRrigo; P. Conte; G. Anzillotti; A. D. Giancamillo; L. D. Girolamo; G. Peretti; A. Crovace; E. Kon. DEVELOPMENT AND VALIDATION OF CLINICALLY RELEVANT ANIMAL MODEL OF MENISCAL DEGENERATION. Orthopaedic Proceedings 2024, 106-B, 123 -123.

D. D’aRrigo, P. Conte, G. Anzillotti, A. D. Giancamillo, L. D. Girolamo, G. Peretti, A. Crovace, E. Kon. DEVELOPMENT AND VALIDATION OF CLINICALLY RELEVANT ANIMAL MODEL OF MENISCAL DEGENERATION. Orthopaedic Proceedings. 2024; 106-B (SUPP_18):123-123.

D. D’aRrigo; P. Conte; G. Anzillotti; A. D. Giancamillo; L. D. Girolamo; G. Peretti; A. Crovace; E. Kon. 2024. "DEVELOPMENT AND VALIDATION OF CLINICALLY RELEVANT ANIMAL MODEL OF MENISCAL DEGENERATION." Orthopaedic Proceedings 106-B, no. SUPP_18: 123-123.

Purpose: In this study, we investigated whether experimental knee pain alters lower limb kinematics and knee arthrokinematics during gait, and if this motor adaptation depends on the spatial characteristics of the painful stimulus. Methods: Twenty-one participants walked on a treadmill for 60-s trials, either without stimulation or while experiencing painful electrical stimulation in the medial, lateral or anterior region of the knee. Perceived pain location was analyzed using pain drawing. Gait spatiotemporal parameters, lower limb kinematics, and dispersion of the knee helical axes on the sagittal plane were quantified for each trial and compared between conditions using ANOVAs with repeated measures or Friedman tests. Results: Pain perception was localized in the area the stimulation was applied to. Compared to walking without pain, participants demonstrated reduced knee extension (1.5 ± 1.5 degrees, p = 0.002) and reduced hip extension (0.8 ± 1.1 degrees, p = 0.037) when pain was induced in the anterior region, but not medially or laterally. Anterior knee pain increased the mean distance of the helical axes during late stance (0.7 [0.3, 1.4], p = 0.010), while medial pain increased both mean distance (0.3 [0.1, 0.5], p = 0.037) and mean angle (1.2 ± 1.4, p = 0.010) during early swing. Conclusion: Acute, experimental knee pain alters gait kinematics and increases the dispersion of the helical axis. These adaptations depend on the spatial characteristics of the painful stimulus. These adaptations may reflect an attempt of the central nervous system to protect the painful tissue while searching for a less painful movement strategy.

Alessio Gallina; Paola Adamo; Giorgia Marino; Corrado Cescon; Francesco Bolzoni; Marco Barbero; Federico Temporiti; Roberto Gatti. Effect of experimental knee pain location on gait kinematics. European Journal of Applied Physiology 2024, 1 -11.

Alessio Gallina, Paola Adamo, Giorgia Marino, Corrado Cescon, Francesco Bolzoni, Marco Barbero, Federico Temporiti, Roberto Gatti. Effect of experimental knee pain location on gait kinematics. European Journal of Applied Physiology. 2024; ():1-11.

Alessio Gallina; Paola Adamo; Giorgia Marino; Corrado Cescon; Francesco Bolzoni; Marco Barbero; Federico Temporiti; Roberto Gatti. 2024. "Effect of experimental knee pain location on gait kinematics." European Journal of Applied Physiology , no. : 1-11.