The aims of this study were to boost growth attributes, yield, and nutrient uptake of rice in paddy fields using a combination of Bacillus pumilus strain TUAT-1 biofertilizer and different nitrogen (N) application rates in nursery boxes. Bacillus pumilus strain TUAT-1 was applied as an inoculant biofertilizer in conjunction with different rates of N fertilizer to rice seedlings in a nursery. Plant growth and yield parameters were evaluated at two stages: in 21-day-old nursery seedlings and in mature rice plants growing in a paddy field. Inoculation with TUAT-1 significantly increased the seedling growth and root morphology of 21-day-old nursery seedlings. There was a marked increase in chlorophyll content, plant height, number of tillers, and tiller biomass of rice plants with the use of TUAT-1 and N fertilizers alone, and their combinations, at the maximum tillering stage in the field. The combination of TUAT-1 and 100% N (farmer recommended rate of N) resulted in the greatest tiller number and biomass at the maximum tillering stage, and positively affected other growth attributes and yield. The growth and yield were similar in the TUAT-1 + 50% N and 100% N (uninoculated) treatments, because TUAT-1 promoted root development, which increased nutrient uptake from the soil. These results suggest that the B. pumilus strain TUAT-1 has a potential to enhance the nutritional uptake of rice by promoting the growth and development of roots.

We investigated the potential dual application of two Bradyrhizobium strains (B. diazoefficiens USDA110 and B. ottawaense SG09) and plant growth-promoting bacteria, PGPB (Pseudomonas spp.: OFT2 and OFT5), to improve nodulation and N₂-fixation in soybean plants. The growth-promoting effects of dual inoculation were observed on plant growth, physiology, and nodulation of soybean under normal conditions compared with plants individually inoculated with either USDA110 or SG09. Both OFT2 and OFT5 promoted N₂-fixation by 11% and 56%, respectively, when dual inoculation with USDA110 and by 76% and 81%, respectively, when dual inoculation with SG09. Salinity stress significantly reduces soybean growth, physiology, nutrient uptake, nodulation, and N₂-fixation. However, these adverse effects were attenuated by the dual inoculation of PGPB and rhizobia depending on the combination of inoculants. In particular, dual inoculation of PGPB with SG09 was more effective in enhancing the salt tolerance of soybean by reducing salt-induced ethylene production and improving nutrient uptake. However, no such effect was observed with the combined inoculation of USDA110 and OFT5. An effective symbiotic association between SG09 and two Pseudomonas bacteria can be considered a beneficial approach to improving the symbiotic efficiency of nodulation and mitigating salinity stress in soybeans.

An assessment of within field spatial variations in grain yield and methane (CH₄) emission was conducted in lowland rice fields of Myanmar. Two successive rice fields (1^st field and 2^nd field) were divided into fertilized and non-fertilized parts and CH₄ measurements were conducted at the inlet, middle and outlet positions of each field. The results showed that CH₄ emissions at non-fertilized parts were higher than those at fertilized part in both rice fields. The average CH₄ emissions ranged from 8.7 to 26.6 mg m^-2 h^-1 in all positions in both rice fields. The spatial variation in CH₄ emission among the positions was high in both rice fields with the highest emissions in the outlet of the 1^st field and the inlet of the 2^nd field. The CH₄ emissions at these two positions showed 2 - 2.5 times higher than those at other positions in both rice fields. Stepwise regression analysis indicates that soil total carbon content is the primary factor for CH₄ emission. The average CH₄ emissions during rice growing season were 13.5 mg m^-2 h^-1 for the 1^st field and 15.7 mg m^-2 h^-1 for the 2^nd field. Spearman rank order correlation analysis showed that CH₄ emission was significantly and positively correlated with soil temperature, surface water depth and negatively correlated with soil redox potential. The result indicated that high within field spatial variation in CH₄ emissions required different site specific management practices to mitigate CH₄ emissions in lowland paddy rice soil.

Two-year field experiments were conducted at Tamil Nadu Rice Research Institute, Aduthurai, Tamil Nadu, India, to evaluate the effect of continuous flooding (CF) and alternate wetting and drying (AWD) irrigation strategies on rice grain yield and greenhouse gas emissions from double-cropping paddy rice. Field observation results showed that AWD irrigation was found to reduce the total seasonal methane (CH₄) emission by 22.3% to 56.2% compared with CF while maintaining rice yield. By using the observed two-year field data, validation of the DNDC-Rice model was conducted for CF and AWD practices. The model overestimated rice grain yield by 24% and 29% in CF and AWD, respectively, averaged over the rice-growing seasons compared to observed values. The simulated seasonal CH₄ emissions for CF were 6.4% lower and 4.2% higher than observed values and for AWD were 9.3% and 12.7% lower in the summer and monsoon season, respectively. The relative deviation of simulated seasonal nitrous oxide (N₂O) emissions from observed emissions in CF were 27% and −35% and in AWD were 267% and 234% in the summer and monsoon season, respectively. Although the DNDC-Rice model reasonably estimated the total CH₄ emission in CF and reproduced the mitigation effect of AWD treatment on CH₄ emissions well, the model did not adequately predict the total N₂O emission under water-saving irrigation. In terms of global warming potential (GWP), nevertheless there was a good agreement between the simulated and observed values for both CF and AWD irrigations due to smaller contributions of N₂O to the GWP compared with that of CH₄. This study showed that the DNDC-Rice model could be used for the estimation of CH₄ emissions, the primary source of GWP from double-cropping paddy rice under different water management conditions in the tropical regions.

A laboratory incubation experiment is conducted for 90 days under controlled conditions where either pruning residue or its biochar is applied to determine which application generates the lowest amount of greenhouse gas from tea plantation soil. To study the effect of incorporation depth on soil N₂O and CO₂ emissions, experiment 1 is performed with three treatments: (1) control; (2) tea pruning residue; and (3) residue biochar mixed with soil from two different depths (0–5 cm and 0–10 cm layers). In experiment 2, only the 0–10 cm soil layer is used to study the effect of surface application of tea pruning residue or its biochar on soil N₂O and CO₂ emissions compared with the control. The results show that biochar significantly increases soil pH, total C and C/N ratio in both experiments. The addition of pruning residue significantly increases soil total C content, cumulative N₂O and CO₂ emissions after 90 days of incubation. Converting pruning residue to biochar and its application significantly decreases cumulative N₂O emission by 17.7% and 74.2% from the 0–5 cm and 0–10 cm soil layers, respectively, compared to their respective controls. However, biochar addition increases soil CO₂ emissions for both the soil layers in experiment 1. Surface application of biochar to soil significantly reduces both N₂O and CO₂ emissions compared to residue treatment and the control in experiment 2. Our results suggest that converting pruning residue to biochar and its addition to soil has the potential to mitigate soil N₂O emissions from tea plantation.