Traditional smoked fermented sausages are highly appreciated in Portugal and are mostly manufactured according to traditional procedures. The aim of the present work was to evaluate the effect of autochthonous starter cultures on the safety and quality of a smoked fermented sausage, Painho da Beira Baixa (PBB), preserving its sensory quality. Physicochemical parameters, namely pH and water activity (a_W), microbiological parameters, biogenic amines, colour, texture profile and sensory attributes were assessed. Different starters were selected based on our previous work. Staphylococcus equorum S2M7, Staphylococcus xylosus CECT7057, Lactobacillus sakei CV3C2, Lactobacillus sakei CECT7056 and a yeast strain (2RB4) were co-inoculated in meat batters at defined concentrations. Starters had a significant effect on the reduction of pH. Enterobacteria and Listeria monocytogenes were not detected in inoculated end-product sausages. Moreover, sausages inoculated with S. equorum S2M7/L. sakei CV3C2/yeast 2RB4 showed a significant reduction in the total content of biogenic amines. No significant differences between treatments were observed for colour and texture parameters, except for adhesiveness. The studied starters did not compromise the sensory characteristics of PBB. To our knowledge, this is the first comprehensive study on the quality and safety of this type of smoked fermented sausage from the central region of Portugal.

In the Mediterranean region, climate change is likely to generate an increase in water demand and the deterioration of its quality. The adoption of precision viticulture and the best available techniques aiming at sustainable production, minimizing the impact on natural resources and reducing production costs, has therefore been a goal of winegrowers. In this work, the water footprint (WFP) in the wine sector was evaluated, from the vineyard to the bottle, through the implementation of a methodology based on field experiments and life cycle assessment (LCA) on two Portuguese case studies. Regarding direct water footprint, it ranged from 366 to 899 L/FU (0.75 L bottle), with green water being the most significant component, representing more than 50% of the overall water footprint. The approach used in the current study revealed that although more than 97.5% of the water footprint is associated with vineyard, the winery stage is responsible for more than 75% of the global warming potential indicator. A linear correlation between the carbon footprint and the indirect blue water footprint was also observed for both case studies. Climate change is expected to cause an earlier and prolonged water stress period, resulting in an increase of about 40% to 82% of blue WFP.

In the Mediterranean region, water scarcity has already prompted concern in the wine sector due to the strong impact it has on vineyard productivity and wine quality. Water footprint is an indicator that takes account of all the water involved in the creation of a product and may help producers to identify hotspots, and reduce water consumption and the corresponding production costs. In recent years several studies have been reported on wine water footprint determination, but mostly focused on the viticulture phase or assuming no grey water footprint at the winery since it has a treatment system. In the framework of the WineWaterFootprint project a medium-size winery was monitored, with direct measurements, regarding determination of the blue and grey components of water footprint. The determined winery water footprint ranged from 9.6 to 12.7 L of water per wine bottle of 0.75 L, the wastewater produced being responsible for about 98%, which means that the grey component cannot be disregarded. The developed scenarios show that a potential reduction of 87% in winery water footprint can be obtained with almost no investment. The challenge of reducing the grey footprint is not in technology development, but rather in the proper maintenance and monitoring of treatment systems.

Paio do Alentejo (PA) is one of the most popular dry-fermented sausages in Portugal. The aim of the present work was to evaluate the effect of a high concentration of starter cultures with an extended fermentation step on the safety and quality of PA. Physicochemical parameters, microbiological parameters, biogenic amines, colour, texture profile, and sensory attributes were assessed. Five starters were selected based on our previous works. Staphylococcus equorum S2M7, Staphylococcus xylosus CECT7057, Lactobacillus sakei CV3C2, Lactobacillus sakei CECT7056, and a yeast strain (2RB4) were co-inoculated in meat batters at a concentration of 10⁸ cfu/g for bacteria and 10⁶ cfu/g for yeast strain, and 0.25% dextrose was added. Inoculated starters significantly reduced pH, Listeria monocytogenes counts, and total content in biogenic amines. The studied starter cultures did not compromise the sensory characteristics of PA, and thus, their use can be considered to protect these sausages and contribute to their safety.

Dry-cured sausages are traditional in Mediterranean countries, and Paio do Alentejo (PA) is one of the most popular in South Portugal. The aim of the present work was to evaluate the effect of combined starters on the safety and quality of PA preserving its sensory quality. Physicochemical parameters, namely pH and water activity (a_W), microbiological parameters, biogenic amines, color, texture, and sensory attributes were assessed. Three starter cultures were used, namely Staphylococcus equorum S2M7 and Lactobacillus sakei CV3C2, both separate and combined with the 2RB4 yeast strain at a concentration of 10⁶ cfu/g. Dextrose 0.25% was added to the meat batter. Starters had a significant effect on the reduction of a_W values (0.845 to 0.823). The treatment with L. sakei as well as the co-inoculation of L. sakei with S. equorum effectively reduced the L. monocytogenes counts to undetectable levels. Sausages co-inoculated with S. equorum S2M7/L. sakei CV3C2 showed a significant reduction in the content of vasoactive amines, namely tryptamine (26.21 to 15.70) and β-phenylethylamine (4.80 to 3.69). Regarding texture, control PA showed higher hardness values, and the starters promoted the cohesiveness of the batter while reducing chewiness. The studied starters did not compromise the sensory characteristics of PA.

Edible insect powder, particularly from the cricket Acheta domesticus L., is a promising sustainable alternative to traditional livestock-derived protein. Insects provide high protein content, fibre, and essential minerals, making them suitable for food applications. This study investigates the viability of alternative diets for rearing A. domesticus. Two experimental diets were tested: RI [50% horticultural by-products (HP) + 50% commercial diet (CD)] and RII (33% HP + 33% CD + 33% fish by-products). The results demonstrated that both diets were suitable for cricket rearing. Crickets reared on diets RI and RII produced, respectively, insect powders FI and FII, which were evaluated for their nutritional, bioactive, and microbiological attributes. Both powders exhibited high protein content (≈60%), all essential amino acids, higher mineral content than traditional protein sources, and met European Union food safety standards. Diet composition influenced powder characteristics: FI showed higher antioxidant activity and saturated fat content, while FII contained more protein, ash, minerals, and monounsaturated fatty acids. These findings underscore the potential of using industrial by-products to promote a circular economy in insect farming and suggest pathways for further research. However, since insects can bioaccumulate toxic elements, such as Hg, from diets, caution should be taken when considering fish by-products.