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Stormwater quality in three urban watersheds in Denver that have been undergoing rapid infill redevelopment for about a decade was evaluated. Sampling was conducted over 18 months, considering 15 storms. Results: (1) The first-flush effect was observed for nutrients and total suspended solids (TSS) but not for total dissolved solids (TDS), conductivity, pH, and fecal indicator bacteria; (2) though no significant differences on event mean concentration (EMC) values were found among the three basins, local-scale EMCs were higher than traditional city-wide standards, particularly some metals and nutrients, most likely because of the significantly higher imperviousness of the studied urban basins compared to city averages; (3) peak rainfall intensity and total rainfall depth showed significant but weak correlations with some nutrients and metals, and TDS; (4) antecedent dry period were not correlated with water quality, except for phosphorus and lead; (5) contrary to what was expected, total coliforms and Escherichia coli were not correlated with TSS; and (6) no significant correlations between water quality and land-use or zoning categories were found. It was concluded that locally focused stormwater monitoring can aid data-driven decision-making by city planners where redevelopment is occurring at local “neighborhood scales”, particularly for the implementation and management related to green infrastructure and water-quality regulations.
Kyle Gustafson; Pablo Garcia-Chevesich; Kimberly Slinski; Jonathan Sharp; John McCray. Quantifying the Effects of Residential Infill Redevelopment on Urban Stormwater Quality in Denver, Colorado. Water 2021, 13, 988 .
AMA StyleKyle Gustafson, Pablo Garcia-Chevesich, Kimberly Slinski, Jonathan Sharp, John McCray. Quantifying the Effects of Residential Infill Redevelopment on Urban Stormwater Quality in Denver, Colorado. Water. 2021; 13 (7):988.
Chicago/Turabian StyleKyle Gustafson; Pablo Garcia-Chevesich; Kimberly Slinski; Jonathan Sharp; John McCray. 2021. "Quantifying the Effects of Residential Infill Redevelopment on Urban Stormwater Quality in Denver, Colorado." Water 13, no. 7: 988.
Projections of increased hydrological extremes due to climate change heighten the need to understand and improve the resilience of our water infrastructure. While constructed natural treatment analogs, such as raingardens, wetlands, and aquifer recharge, hold intuitive promise for variable flows, the impacts of disruption on water treatment processes and outcomes are not well understood and limit widespread adoption. To this end, we studied the impact of desiccation and flooding extremes on demonstration-scale shallow, unit process open water (UPOW) wetlands designed for water treatment. System resilience was evaluated as a function of physical characteristics, nitrate removal, photosynthetic activity, and microbial ecology. Rehydrated biomat that had been naturally desiccated re-established nitrate removal consistent with undisrupted biomat in less than a week; however, a pulse of organic carbon and nitrogen accompanied the initial rehydration phase. Conversely, sediment intrusion due to flooding had a negative impact on the biomat’s photosynthetic activity and decreased nitrate attenuation rates by nearly 50%. Based upon past mechanistic inferences, attenuation potential for trace organics is anticipated to follow similar trends as nitrate removal. While the microbial community was significantly altered in both extremes, our results collectively suggest that UPOW wetlands have potential for seasonal or intermittent use due to their promise of rapid re-establishment after rehydration. Flooding extremes and associated sediment intrusion provide a greater barrier to system resilience indicating a need for proactive designs to prevent this outcome; however, residual treatment potential after disruption could provide operators with time to triage and manage the system should a flood occur again.
Adam Brady; Michael Vega; Kimberly Riddle; Henry Peel; Evelyn Lundeen; Julia Siegmund; Jonathan Sharp. Biomat Resilience to Desiccation and Flooding Within a Shallow, Unit Process Open Water Engineered Wetland. Water 2021, 13, 815 .
AMA StyleAdam Brady, Michael Vega, Kimberly Riddle, Henry Peel, Evelyn Lundeen, Julia Siegmund, Jonathan Sharp. Biomat Resilience to Desiccation and Flooding Within a Shallow, Unit Process Open Water Engineered Wetland. Water. 2021; 13 (6):815.
Chicago/Turabian StyleAdam Brady; Michael Vega; Kimberly Riddle; Henry Peel; Evelyn Lundeen; Julia Siegmund; Jonathan Sharp. 2021. "Biomat Resilience to Desiccation and Flooding Within a Shallow, Unit Process Open Water Engineered Wetland." Water 13, no. 6: 815.
Open-water wetlands are a novel treatment technology for reverse osmosis concentrate that reduced nitrate concentrations in a pilot-scale system.
Rachel C. Scholes; Michael A. Vega; Jonathan O. Sharp; David L. Sedlak. Nitrate removal from reverse osmosis concentrate in pilot-scale open-water unit process wetlands. Environmental Science: Water Research & Technology 2021, 7, 650 -661.
AMA StyleRachel C. Scholes, Michael A. Vega, Jonathan O. Sharp, David L. Sedlak. Nitrate removal from reverse osmosis concentrate in pilot-scale open-water unit process wetlands. Environmental Science: Water Research & Technology. 2021; 7 (3):650-661.
Chicago/Turabian StyleRachel C. Scholes; Michael A. Vega; Jonathan O. Sharp; David L. Sedlak. 2021. "Nitrate removal from reverse osmosis concentrate in pilot-scale open-water unit process wetlands." Environmental Science: Water Research & Technology 7, no. 3: 650-661.
Adam Brady; Carly Oliver; Michael Vega; Jonathan Sharp; Julia Siegmund; Evelyn Lundeen; Dana Sanelli. Resilience of photosynthetic biomat within a unit process open water wetland following extreme events. 2020, 1 .
AMA StyleAdam Brady, Carly Oliver, Michael Vega, Jonathan Sharp, Julia Siegmund, Evelyn Lundeen, Dana Sanelli. Resilience of photosynthetic biomat within a unit process open water wetland following extreme events. . 2020; ():1.
Chicago/Turabian StyleAdam Brady; Carly Oliver; Michael Vega; Jonathan Sharp; Julia Siegmund; Evelyn Lundeen; Dana Sanelli. 2020. "Resilience of photosynthetic biomat within a unit process open water wetland following extreme events." , no. : 1.
There is interest in using locally available, low cost organic materials to attenuate heavy metals such as Cd, Cr, Cu, Hg, Ni, Pb, and Zn found in surface waters in Peru and other developing regions. Here we mesh Spanish language publications, archived theses, and prior globally available literature to provide a tabulated synthesis of organic materials that hold promise for this application in the developing world. In total, nearly 200 materials were grouped into source categories such as algae and seashells, bacteria and fungi, terrestrial plant-derived materials, and other agricultural and processing materials. This curation was complemented by an assessment of removal potential that can serve as a resource for future studies. We also identified a subset of Peruvian materials that hold particular promise for further investigation, including seashell-based mixed media, fungal blends, lignocellulose-based substrates including sawdust, corn and rice husks, and food residuals including peels from potatoes and avocadoes. Many studies reported percent removal and/or lacked consistent protocols for solid to liquid ratios and defined aqueous concentrations, which limits direct application. However, they hold value as an initial screening methodology informed by local knowledge and insights that could enable adoption for agriculture and other non-potable water reuse applications. While underlying removal mechanisms were presumed to rely on sorptive processes, this should be confirmed in promising materials with subsequent experimentation to quantify active sites and capacities by generating sorption isotherms with a focus on environmental conditions and specific contaminated water properties (pH, temperature, ionic strength, etc.). These organics also hold promise for the pairing of sorption to indirect microbial respiratory processes such as biogenic sulfide complexation. Conversely, there is a need to quantify unwanted contaminant release that could include soluble organic matter and nutrients. In addition to local availability and treatment efficacy, social, technical, economic, and environmental applicability of those materials for large-scale application must be considered to further refine material selection.
Pablo Garcia-Chevesich; Vilma García; Gisella Martínez; Julia Zea; Juana Ticona; Francisco Alejo; Johan Vanneste; Sarah Acker; Gary Vanzin; Aaron Malone; Nicole M. Smith; Christopher Bellona; Jonathan O. Sharp. Inexpensive Organic Materials and Their Applications towards Heavy Metal Attenuation in Waters from Southern Peru. Water 2020, 12, 2948 .
AMA StylePablo Garcia-Chevesich, Vilma García, Gisella Martínez, Julia Zea, Juana Ticona, Francisco Alejo, Johan Vanneste, Sarah Acker, Gary Vanzin, Aaron Malone, Nicole M. Smith, Christopher Bellona, Jonathan O. Sharp. Inexpensive Organic Materials and Their Applications towards Heavy Metal Attenuation in Waters from Southern Peru. Water. 2020; 12 (10):2948.
Chicago/Turabian StylePablo Garcia-Chevesich; Vilma García; Gisella Martínez; Julia Zea; Juana Ticona; Francisco Alejo; Johan Vanneste; Sarah Acker; Gary Vanzin; Aaron Malone; Nicole M. Smith; Christopher Bellona; Jonathan O. Sharp. 2020. "Inexpensive Organic Materials and Their Applications towards Heavy Metal Attenuation in Waters from Southern Peru." Water 12, no. 10: 2948.
Microbial genetic potential for the biotransformation of xenobiotics and antibiotic resistance increases with depth during biofiltration.
Dong Li; Jonathan O. Sharp; Jörg E. Drewes. Microbial genetic potential for xenobiotic metabolism increases with depth during biofiltration. Environmental Science: Processes & Impacts 2020, 22, 2058 -2069.
AMA StyleDong Li, Jonathan O. Sharp, Jörg E. Drewes. Microbial genetic potential for xenobiotic metabolism increases with depth during biofiltration. Environmental Science: Processes & Impacts. 2020; 22 (10):2058-2069.
Chicago/Turabian StyleDong Li; Jonathan O. Sharp; Jörg E. Drewes. 2020. "Microbial genetic potential for xenobiotic metabolism increases with depth during biofiltration." Environmental Science: Processes & Impacts 22, no. 10: 2058-2069.
Jonathan Sharp; Lawrence Berkeley National Laboratory. Final Report: Mechanistic and predictive understanding of needle litter decay in semi-arid mountain ecosystems experiencing unprecedented vegetation mortality. Final Report: Mechanistic and predictive understanding of needle litter decay in semi-arid mountain ecosystems experiencing unprecedented vegetation mortality 2020, 1 .
AMA StyleJonathan Sharp, Lawrence Berkeley National Laboratory. Final Report: Mechanistic and predictive understanding of needle litter decay in semi-arid mountain ecosystems experiencing unprecedented vegetation mortality. Final Report: Mechanistic and predictive understanding of needle litter decay in semi-arid mountain ecosystems experiencing unprecedented vegetation mortality. 2020; ():1.
Chicago/Turabian StyleJonathan Sharp; Lawrence Berkeley National Laboratory. 2020. "Final Report: Mechanistic and predictive understanding of needle litter decay in semi-arid mountain ecosystems experiencing unprecedented vegetation mortality." Final Report: Mechanistic and predictive understanding of needle litter decay in semi-arid mountain ecosystems experiencing unprecedented vegetation mortality , no. : 1.
This study investigates the isolated decomposition of spruce and lodgepole conifer needles to enhance our understanding of how needle litter impacts near-surface terrestrial biogeochemical processes. Harvested needles were exported to a subalpine meadow to enable a discrete analysis of the decomposition processes over 2 years. Initial chemistry revealed the lodgepole needles to be less recalcitrant with a lower carbon to nitrogen (C:N) ratio. Total C and N fundamentally shifted within needle species over time with decreased C:N ratios for spruce and increased ratios for lodgepole. Differences in chemistry correlated with CO2 production and soil microbial communities. The most pronounced trends were associated with lodgepole needles in comparison to the spruce and needle-free controls. Increased organic carbon and nitrogen concentrations associated with needle presence in soil extractions further corroborate the results with clear biogeochemical signatures in association with needle chemistry. Interestingly, no clear differentiation was observed as a function of bark beetle impacted spruce needles vs those derived from healthy spruce trees despite initial differences in needle chemistry. These results reveal that the inherent chemistry associated with tree species has a greater impact on soil biogeochemical signatures during isolated needle decomposition. By extension, biogeochemical shifts associated with bark beetle infestation are likely driven more by changes such as the cessation of rhizospheric processes than by needle litter decomposition.
Laura T. Leonard; Kristin Mikkelson; Zhao Hao; Eoin L. Brodie; Kenneth H. Williams; Jonathan O. Sharp. A comparison of lodgepole and spruce needle chemistry impacts on terrestrial biogeochemical processes during isolated decomposition. PeerJ 2020, 8, e9538 .
AMA StyleLaura T. Leonard, Kristin Mikkelson, Zhao Hao, Eoin L. Brodie, Kenneth H. Williams, Jonathan O. Sharp. A comparison of lodgepole and spruce needle chemistry impacts on terrestrial biogeochemical processes during isolated decomposition. PeerJ. 2020; 8 ():e9538.
Chicago/Turabian StyleLaura T. Leonard; Kristin Mikkelson; Zhao Hao; Eoin L. Brodie; Kenneth H. Williams; Jonathan O. Sharp. 2020. "A comparison of lodgepole and spruce needle chemistry impacts on terrestrial biogeochemical processes during isolated decomposition." PeerJ 8, no. : e9538.
Field studies that simulate the effects of climate change are important for a predictive understanding of ecosystem responses to a changing environment. Among many concerns, regional warming can result in advanced timing of spring snowmelt in snowpack dependent ecosystems, which could lead to longer snow-free periods and drier summer soils. Past studies investigating these impacts of climate change have manipulated snowmelt with a variety of techniques that include manual snowpack alteration with a shovel, infrared radiation, black sand and fabric covers. Within these studies however, sufficient documentation of methods is limited, which can make experimental reproduction difficult. Here, we outline a detailed plot-scale protocol that utilizes a permeable black geotextile fabric deployed on top of an isothermal spring snowpack to induce advanced snowmelt. The method offers a reliable and cost-effective approach to induce snowmelt by passively increasing solar radiation absorption at the snow surface. In addition, control configurations with no snowpack manipulation are paired adjacent to the induced snowmelt plot for experimental comparison. Past and ongoing deployments in Colorado subalpine ecosystems indicate that this approach can accelerate snowmelt by 14-23 days, effectively mimicking snowmelt timing at lower elevations. This protocol can be applied to a variety of studies to understand the hydrological, ecological, and geochemical impacts of regional warming in snowpack dependent ecosystems.
Laura T. Leonard; Chelsea Wilmer; Heidi Steltzer; Kenneth H. Williams; Jonathan O. Sharp. Accelerated Snowmelt Protocol to Simulate Climate Change Induced Impacts on Snowpack Dependent Ecosystems. BIO-PROTOCOL 2020, 10, e3557 -e3557.
AMA StyleLaura T. Leonard, Chelsea Wilmer, Heidi Steltzer, Kenneth H. Williams, Jonathan O. Sharp. Accelerated Snowmelt Protocol to Simulate Climate Change Induced Impacts on Snowpack Dependent Ecosystems. BIO-PROTOCOL. 2020; 10 (6):e3557-e3557.
Chicago/Turabian StyleLaura T. Leonard; Chelsea Wilmer; Heidi Steltzer; Kenneth H. Williams; Jonathan O. Sharp. 2020. "Accelerated Snowmelt Protocol to Simulate Climate Change Induced Impacts on Snowpack Dependent Ecosystems." BIO-PROTOCOL 10, no. 6: e3557-e3557.
This paper presents a novel method to couple an environmental bioremediation system with a subsurface renewable energy storage system. This method involves treating unsaturated contaminated soil using in-situ thermally enhanced bioremediation; the thermal system is powered by renewable energy. After remediation goals are achieved, the thermal system can then be used to store renewable energy in the form of heat in the subsurface for later use. This method can be used for enhanced treatment of environmental pollutants for which temperature is considered a limiting factor. For instance, this system can be used at a wide variety of petroleum-related sites that are likely contaminated with hydrocarbons such as oil refineries and facilities with above- and underground storage tanks. In this paper, a case-study example was analyzed using a previously developed numerical model of heat transfer in unsaturated soil. Results demonstrate that coupling energy storage and thermally-enhanced bioremediation systems offer an efficient and sustainable way to achieve desired temperature–moisture distribution in soil that will ultimately enhance the microbial activity.
Ali Moradi; Kathleen M. Smits; Jonathan O. Sharp. Coupled Thermally-Enhanced Bioremediation and Renewable Energy Storage System: Conceptual Framework and Modeling Investigation. Water 2018, 10, 1288 .
AMA StyleAli Moradi, Kathleen M. Smits, Jonathan O. Sharp. Coupled Thermally-Enhanced Bioremediation and Renewable Energy Storage System: Conceptual Framework and Modeling Investigation. Water. 2018; 10 (10):1288.
Chicago/Turabian StyleAli Moradi; Kathleen M. Smits; Jonathan O. Sharp. 2018. "Coupled Thermally-Enhanced Bioremediation and Renewable Energy Storage System: Conceptual Framework and Modeling Investigation." Water 10, no. 10: 1288.
The widespread adoption of engineered wetlands designed for water treatment is hindered by uncertainties in system reliability, resilience and management associated with coupled biological and physical processes. To better understand how shallow unit process open-water wetlands self-colonize and evolve, we analyzed the composition of the microbial community in benthic biomats from system establishment through approximately 3 years of operation. Our analysis was conducted across three parallel demonstration-scale (7500 m) cells located within the Prado Constructed Wetlands in Southern California. They received water from the Santa Ana River (5.9 ± 0.2 mg/L NO-N), a water body where the flow is dominated by municipal wastewater effluent from May to November. Phylogenetic inquiry and microscopy confirmed that diatoms and an associated aerobic bacterial community facilitated early colonization. After approximately nine months of operation, coinciding with late summer, an anaerobic community emerged with the capability for nitrate attenuation. Varying the hydraulic residence time (HRT) from 1 to 4 days the subsequent year resulted in modest ecological changes across the three parallel cells that were most evident in the outlet regions of the cells. The community that established at this time was comparatively stable for the remaining years of operation and converged with one that had previously formed approximately 550 km (350 miles) away in a pilot-scale (400 m) wetland in Northern California. That system received denitrified (20.7 ± 0.7 mg/L NO-N), secondary treated municipal wastewater for 5 years of operation. Establishment of a core microbiome between the two systems revealed a strong overlap of both aerobic and anaerobic taxa with approximately 50% of the analyzed bacterial sequences shared between the two sites. Additionally the same species of diatom, Stauirsa construens var. venter, was prolific in both systems as the putative dominant primary producer. Our results indicate that despite differences in scale, geographic location and source waters, the shallow open-water wetland design can select for a rapid convergence of microbial structure and functionality associated with the self-colonizing benthic biomat. This resulting biomat matures over the first growing season with operational parameters such as HRT further exerting a modest selective bias on community succession.
Zackary L. Jones; Kristin M. Mikkelson; Scott Nygren; David L. Sedlak; Jonathan O. Sharp. Establishment and convergence of photosynthetic microbial biomats in shallow unit process open-water wetlands. Water Research 2018, 133, 132 -141.
AMA StyleZackary L. Jones, Kristin M. Mikkelson, Scott Nygren, David L. Sedlak, Jonathan O. Sharp. Establishment and convergence of photosynthetic microbial biomats in shallow unit process open-water wetlands. Water Research. 2018; 133 ():132-141.
Chicago/Turabian StyleZackary L. Jones; Kristin M. Mikkelson; Scott Nygren; David L. Sedlak; Jonathan O. Sharp. 2018. "Establishment and convergence of photosynthetic microbial biomats in shallow unit process open-water wetlands." Water Research 133, no. : 132-141.
Open-water unit process wetlands host a benthic diatomaceous and bacterial assemblage capable of nitrate removal from treated municipal wastewater with unexpected contributions from anammox processes. In exploring mechanistic drivers of anammox, 16S rRNA gene sequencing profiles of the biomat revealed significant microbial community shifts along the flow path and with depth. Notably, there was an increasing abundance of sulfate reducers ( Desulfococcus and other Deltaproteobacteria ) and anammox microorganisms ( Brocadiaceae ) with depth. Pore water profiles demonstrated that nitrate and sulfate concentrations exhibited a commensurate decrease with biomat depth accompanied by the accumulation of ammonium. Quantitative PCR targeting the anammox hydrazine synthase gene, hzsA , revealed a 3-fold increase in abundance with biomat depth as well as a 2-fold increase in the sulfate reductase gene, dsrA . These microbial and geochemical trends were most pronounced in proximity to the influent region of the wetland where the biomat was thickest and influent nitrate concentrations were highest. While direct genetic queries for dissimilatory nitrate reduction to ammonium (DNRA) microorganisms proved unsuccessful, an increasing depth-dependent dominance of Gammaproteobacteria and diatoms that have previously been functionally linked to DNRA was observed. To further explore this potential, a series of microcosms containing field-derived biomat material confirmed the ability of the community to produce sulfide and reduce nitrate; however, significant ammonium production was observed only in the presence of hydrogen sulfide. Collectively, these results suggest that biogenic sulfide induces DNRA, which in turn can explain the requisite coproduction of ammonium and nitrite from nitrified effluent necessary to sustain the anammox community. IMPORTANCE This study aims to increase understanding of why and how anammox is occurring in an engineered wetland with limited exogenous contributions of ammonium and nitrite. In doing so, the study has implications for how geochemical parameters could potentially be leveraged to impact nutrient cycling and attenuation during the operation of treatment wetlands. The work also contributes to ongoing discussions about biogeochemical signatures surrounding anammox processes and enhances our understanding of the contributions of anammox processes in freshwater environments.
Zackary Jones; Justin T. Jasper; David L. Sedlak; Jonathan O. Sharp. Sulfide-Induced Dissimilatory Nitrate Reduction to Ammonium Supports Anaerobic Ammonium Oxidation (Anammox) in an Open-Water Unit Process Wetland. Applied and Environmental Microbiology 2017, 83, e00782-17 .
AMA StyleZackary Jones, Justin T. Jasper, David L. Sedlak, Jonathan O. Sharp. Sulfide-Induced Dissimilatory Nitrate Reduction to Ammonium Supports Anaerobic Ammonium Oxidation (Anammox) in an Open-Water Unit Process Wetland. Applied and Environmental Microbiology. 2017; 83 (15):e00782-17.
Chicago/Turabian StyleZackary Jones; Justin T. Jasper; David L. Sedlak; Jonathan O. Sharp. 2017. "Sulfide-Induced Dissimilatory Nitrate Reduction to Ammonium Supports Anaerobic Ammonium Oxidation (Anammox) in an Open-Water Unit Process Wetland." Applied and Environmental Microbiology 83, no. 15: e00782-17.
Sulfate-reducing bioreactors (SRBRs) represent a passive, sustainable, and long-term option for mitigating mining influenced water (MIW) during release. Here we investigate spatial zinc precipitation profiles as influenced by substrate differentiation, inorganic ligand availability (inorganic carbon and sulfide), and microbial community structure in pilot-scale SRBR columns fed with sulfate and zinc-rich MIW. Through a combination of aqueous sampling, geochemical digests, electron microscopy and energy-dispersive x-ray spectroscopy, we were able to delineate zones of enhanced zinc removal, identify precipitates of varying stability, and discern the temporal and spatial evolution of zinc, sulfur, and calcium associations. These geochemical insights revealed spatially variable immobilization regimes between SRBR columns that could be further contrasted as a function of labile (alfalfa-dominated) versus recalcitrant (woodchip-dominated) solid-phase substrate content. Both column subsets exhibited initial zinc removal as carbonates; however precipitation in association with labile substrates was more pronounced and dominated by metal-sulfide formation in the upper portions of the down flow columns with micrographs visually suggestive of sphalerite (ZnS). In contrast, a more diffuse and lower mass of zinc precipitation in the presence of gypsum-like precipitates occurred within the more recalcitrant column systems. While removal and sulfide-associated precipitation were spatially variable, whole bacterial community structure (ANOSIM) and diversity estimates were comparatively homogeneous. However, two phyla exhibited a potentially selective relationship with a significant positive correlation between the ratio of Firmicutes to Bacteroidetes and sulfide-bound zinc. Collectively these biogeochemical insights indicate that depths of maximal zinc sulfide precipitation are temporally dynamic, influenced by substrate composition and broaden our understanding of bio-immobilized zinc species, microbial interactions and potential operational and monitoring tools in these types of passive bioreactors.
Dina M. Drennan; Robert Almstrand; Jeffrey Ladderud; Ilsu Lee; Lee Landkamer; Linda Figueroa; Jonathan O. Sharp. Spatial impacts of inorganic ligand availability and localized microbial community structure on mitigation of zinc laden mine water in sulfate-reducing bioreactors. Water Research 2017, 115, 50 -59.
AMA StyleDina M. Drennan, Robert Almstrand, Jeffrey Ladderud, Ilsu Lee, Lee Landkamer, Linda Figueroa, Jonathan O. Sharp. Spatial impacts of inorganic ligand availability and localized microbial community structure on mitigation of zinc laden mine water in sulfate-reducing bioreactors. Water Research. 2017; 115 ():50-59.
Chicago/Turabian StyleDina M. Drennan; Robert Almstrand; Jeffrey Ladderud; Ilsu Lee; Lee Landkamer; Linda Figueroa; Jonathan O. Sharp. 2017. "Spatial impacts of inorganic ligand availability and localized microbial community structure on mitigation of zinc laden mine water in sulfate-reducing bioreactors." Water Research 115, no. : 50-59.
Water treatment combining advanced oxidative processes with subsequent exposure to biological activated carbon (BAC) holds promise for the attenuation of recalcitrant pollutants. Here we contrast oxidation and subsequent biofiltration of treated wastewater effluent employing either ozone or UV/H2O2 followed by BAC during pilot-scale implementation. Both treatment trains largely met target water quality goals by facilitating the removal of a suite of trace organics and bulk water parameters. N-nitrosodimethylamine (NDMA) formation was observed in ozone fed BAC columns during biofiltration and to a lesser extent in UV/H2O2 fed columns and was most pronounced at 20 min of empty bed contact time (EBCT) when compared to shorter EBCTs evaluated. While microbial populations were highly similar in the upper reaches, deeper samples revealed a divergence within and between BAC filtration systems where EBCT was identified to be a significant environmental predictor for shifts in microbial populations. The abundance of Nitrospira in the top samples of both columns provides an explanation for the oxidation of nitrite and corresponding increases in nitrate concentrations during BAC transit and support interplay between nitrogen cycling with nitrosamine formation. The results of this study demonstrate that pretreatments using ozone versus UV/H2O2 impart modest differences to the overall BAC microbial population structural and functional attributes, and further highlight the need to evaluate NDMA formation prior to full-scale implementation of BAC in potable reuse applications.
Dong Li; Ben Stanford; Eric Dickenson; Wendell O. Khunjar; Carissa L. Homme; Erik J. Rosenfeldt; Jonathan O. Sharp. Effect of advanced oxidation on N-nitrosodimethylamine (NDMA) formation and microbial ecology during pilot-scale biological activated carbon filtration. Water Research 2017, 113, 160 -170.
AMA StyleDong Li, Ben Stanford, Eric Dickenson, Wendell O. Khunjar, Carissa L. Homme, Erik J. Rosenfeldt, Jonathan O. Sharp. Effect of advanced oxidation on N-nitrosodimethylamine (NDMA) formation and microbial ecology during pilot-scale biological activated carbon filtration. Water Research. 2017; 113 ():160-170.
Chicago/Turabian StyleDong Li; Ben Stanford; Eric Dickenson; Wendell O. Khunjar; Carissa L. Homme; Erik J. Rosenfeldt; Jonathan O. Sharp. 2017. "Effect of advanced oxidation on N-nitrosodimethylamine (NDMA) formation and microbial ecology during pilot-scale biological activated carbon filtration." Water Research 113, no. : 160-170.
Dina Drennan; Robert Almstrand; Ilsu Lee; Lee Landkamer; Linda Figueroa; Jonathan Sharp. Pilot-scale Columns Equipped with Aqueous and Solid-phase Sampling Ports Enable Geochemical and Molecular Microbial Investigations of Anoxic Biological Processes. BIO-PROTOCOL 2017, 7, 1 .
AMA StyleDina Drennan, Robert Almstrand, Ilsu Lee, Lee Landkamer, Linda Figueroa, Jonathan Sharp. Pilot-scale Columns Equipped with Aqueous and Solid-phase Sampling Ports Enable Geochemical and Molecular Microbial Investigations of Anoxic Biological Processes. BIO-PROTOCOL. 2017; 7 (1):1.
Chicago/Turabian StyleDina Drennan; Robert Almstrand; Ilsu Lee; Lee Landkamer; Linda Figueroa; Jonathan Sharp. 2017. "Pilot-scale Columns Equipped with Aqueous and Solid-phase Sampling Ports Enable Geochemical and Molecular Microbial Investigations of Anoxic Biological Processes." BIO-PROTOCOL 7, no. 1: 1.
The recent bark beetle epidemic across western North America may impact water quality as a result of elevated organic carbon release and hydrologic shifts associated with extensive tree dieback. Analysis of quarterly municipal monitoring data from 2004 to 2014 with discretization of six water treatment facilities in the Rocky Mountains by extent of beetle impact revealed a significant increasing trend in total organic carbon (TOC) and total trihalomethane (TTHM) production within high (≳50% areal infestation) beetle-impacted watersheds while no or insignificant trends were found in watersheds with lower impact levels. Alarmingly, the TTHM concentration trend in the high impact sites exceeded regulatory maximum contaminant levels during the most recent two years of analysis (2013-14). To evaluate seasonal differences, explore the interplay of water quality and hydrologic processes, and eliminate variability associated with municipal reporting, these treatment facilities were targeted for more detailed surface water sampling and characterization. Surface water samples collected from high impact watersheds exhibited significantly higher TOC, aromatic signatures, and disinfection byproduct (DBP) formation potential than watersheds with lower infestation levels. Spectroscopic analyses of surface water samples indicated that these heightened DBP precursor levels are a function of both elevated TOC loading and increased aromatic character. This association was heightened during precipitation and runoff events in high impact sites, supporting the hypothesis that altered hydrologic flow paths resulting from tree mortality mobilize organic carbon and elevate DBP formation potential for several months after runoff ceases. The historical trends found here likely underestimate the full extent of TTHM shifts due to monitoring biases with the extended seasonal release of DBP precursors increasing the potential for human exposure. Collectively, our analysis suggests that while water quality impacts continue to rise nearly one decade after infestation, significant increases in TOC mobilization and DBP precursors are limited to watersheds that experience extensive tree mortality.
Brent M. Brouillard; Eric Dickenson; Kristin M. Mikkelson; Jonathan O. Sharp. Water quality following extensive beetle-induced tree mortality: Interplay of aromatic carbon loading, disinfection byproducts, and hydrologic drivers. Science of The Total Environment 2016, 572, 649 -659.
AMA StyleBrent M. Brouillard, Eric Dickenson, Kristin M. Mikkelson, Jonathan O. Sharp. Water quality following extensive beetle-induced tree mortality: Interplay of aromatic carbon loading, disinfection byproducts, and hydrologic drivers. Science of The Total Environment. 2016; 572 ():649-659.
Chicago/Turabian StyleBrent M. Brouillard; Eric Dickenson; Kristin M. Mikkelson; Jonathan O. Sharp. 2016. "Water quality following extensive beetle-induced tree mortality: Interplay of aromatic carbon loading, disinfection byproducts, and hydrologic drivers." Science of The Total Environment 572, no. : 649-659.
Syntrophic relationships between fermentative and sulfate-reducing bacteria are essential to lignocellulose-based systems applied to the passive remediation of mining-influenced waters. In this study, seven pilot-scale sulfate-reducing bioreactor columns containing varying ratios of alfalfa hay, pine woodchips, and sawdust were analyzed over ∼500 days to investigate the influence of substrate composition on zinc removal and microbial community structure. Columns amended with >10% alfalfa removed significantly more sulfate and zinc than did wood-based columns. Enumeration of sulfate reducers by functional signatures (dsrA) and their putative identification from 16S rRNA genes did not reveal significant correlations with zinc removal, suggesting limitations in this directed approach. In contrast, a strong indicator of zinc removal was discerned in comparing the relative abundance of core microorganisms shared by all reactors (>80% of total community), many of which had little direct involvement in metal or sulfate respiration. The relative abundance of Desulfosporosinus, the dominant putative sulfate reducer within these reactors, correlated to representatives of this core microbiome. A subset of these clades, including Treponema, Weissella, and Anaerolinea, was associated with alfalfa and zinc removal, and the inverse was found for a second subset whose abundance was associated with wood-based columns, including Ruminococcus, Dysgonomonas, and Azospira. The construction of a putative metabolic flowchart delineated syntrophic interactions supporting sulfate reduction and suggests that the production of and competition for secondary fermentation byproducts, such as lactate scavenging, influence bacterial community composition and reactor efficacy.
Dina M. Drennan; Robert Almstrand; Ilsu Lee; Lee Landkamer; Linda Figueroa; Jonathan O. Sharp. Organoheterotrophic Bacterial Abundance Associates with Zinc Removal in Lignocellulose-Based Sulfate-Reducing Systems. Environmental Science & Technology 2015, 50, 378 -387.
AMA StyleDina M. Drennan, Robert Almstrand, Ilsu Lee, Lee Landkamer, Linda Figueroa, Jonathan O. Sharp. Organoheterotrophic Bacterial Abundance Associates with Zinc Removal in Lignocellulose-Based Sulfate-Reducing Systems. Environmental Science & Technology. 2015; 50 (1):378-387.
Chicago/Turabian StyleDina M. Drennan; Robert Almstrand; Ilsu Lee; Lee Landkamer; Linda Figueroa; Jonathan O. Sharp. 2015. "Organoheterotrophic Bacterial Abundance Associates with Zinc Removal in Lignocellulose-Based Sulfate-Reducing Systems." Environmental Science & Technology 50, no. 1: 378-387.
Biologically activated carbon column experiments were run with propane-amended and un-amended influents to determine how the microbial community responds to this form of biostimulation and the implications for contaminant attenuation.
Kristin M. Mikkelson; Carissa L. Homme; Dong Li; Jonathan O. Sharp. Propane biostimulation in biologically activated carbon (BAC) selects for bacterial clades adept at degrading persistent water pollutants. Environmental Science: Processes & Impacts 2015, 17, 1405 -1414.
AMA StyleKristin M. Mikkelson, Carissa L. Homme, Dong Li, Jonathan O. Sharp. Propane biostimulation in biologically activated carbon (BAC) selects for bacterial clades adept at degrading persistent water pollutants. Environmental Science: Processes & Impacts. 2015; 17 (8):1405-1414.
Chicago/Turabian StyleKristin M. Mikkelson; Carissa L. Homme; Dong Li; Jonathan O. Sharp. 2015. "Propane biostimulation in biologically activated carbon (BAC) selects for bacterial clades adept at degrading persistent water pollutants." Environmental Science: Processes & Impacts 17, no. 8: 1405-1414.
Large-scale tree mortality has the potential to alter subsurface metal mobility and accumulation as demonstrated by laboratory column studies and complimentary field sampling.
Kristin M. Mikkelson; Lindsay Bearup; Alexis Sitchler; John McCray; Jonathan O. Sharp. Changes in metal mobility associated with bark beetle-induced tree mortality. Environmental Science: Processes & Impacts 2014, 16, 1318 -1327.
AMA StyleKristin M. Mikkelson, Lindsay Bearup, Alexis Sitchler, John McCray, Jonathan O. Sharp. Changes in metal mobility associated with bark beetle-induced tree mortality. Environmental Science: Processes & Impacts. 2014; 16 (6):1318-1327.
Chicago/Turabian StyleKristin M. Mikkelson; Lindsay Bearup; Alexis Sitchler; John McCray; Jonathan O. Sharp. 2014. "Changes in metal mobility associated with bark beetle-induced tree mortality." Environmental Science: Processes & Impacts 16, no. 6: 1318-1327.
Jonathan O. Sharp; Juan S. Lezama-Pacheco; Eleanor J. Schofield; Pilar Junier; Kai-Uwe Ulrich; Satya Chinni; Harish Veeramani; Camille Margot-Roquier; Samuel M. Webb; Bradley Tebo; Daniel E. Giammar; John R. Bargar; Rizlan Bernier-Latmani. Uranium speciation and stability after reductive immobilization in aquifer sediments. Geochimica et Cosmochimica Acta 2011, 75, 6497 -6510.
AMA StyleJonathan O. Sharp, Juan S. Lezama-Pacheco, Eleanor J. Schofield, Pilar Junier, Kai-Uwe Ulrich, Satya Chinni, Harish Veeramani, Camille Margot-Roquier, Samuel M. Webb, Bradley Tebo, Daniel E. Giammar, John R. Bargar, Rizlan Bernier-Latmani. Uranium speciation and stability after reductive immobilization in aquifer sediments. Geochimica et Cosmochimica Acta. 2011; 75 (21):6497-6510.
Chicago/Turabian StyleJonathan O. Sharp; Juan S. Lezama-Pacheco; Eleanor J. Schofield; Pilar Junier; Kai-Uwe Ulrich; Satya Chinni; Harish Veeramani; Camille Margot-Roquier; Samuel M. Webb; Bradley Tebo; Daniel E. Giammar; John R. Bargar; Rizlan Bernier-Latmani. 2011. "Uranium speciation and stability after reductive immobilization in aquifer sediments." Geochimica et Cosmochimica Acta 75, no. 21: 6497-6510.