Global warming is not only affecting arid and semi-arid regions but also becoming a threat to agriculture in Central and Eastern European countries. The present study analyzes the responses to drought and salinity of two varieties of Lavandula angustifolia cultivated in Romania. Lavender seedlings were subjected to one month of salt stress (100, 200, and 300 mM NaCl) and water deficit (complete withholding of irrigation) treatments. To assess the effects of stress on the plants, several growth parameters and biochemical stress markers (photosynthetic pigments, mono and divalent ions, and different osmolytes) were determined in control and stressed plants after the treatments. Both stress conditions significantly inhibited the growth of the two varieties, but all plants survived the treatments, indicating a relative stress tolerance of the two varieties. The most relevant mechanisms of salt tolerance are based on the maintenance of foliar K⁺ levels and the accumulation of Ca²⁺ and proline as a functional osmolyte in parallel with increasing external salinities. Under water stress, significant increases of Na⁺ and K⁺ concentrations were detected in roots, indicating a possible role of these cations in osmotic adjustment, limiting root dehydration. No significant differences were found when comparing the stress tolerance and stress responses of the two selected lavender varieties.

Ornamental woody shrubs are used in landscape design worldwide. Their propagation can be made generatively and vegetatively. Vegetative propagation methods are mostly used by nurseries, as such methods are quick and the newly propagated plants inherit the genetics of the mother plant. However, rooting in some woody plants is slow and, unfortunately, sometimes produces only a small number of rooted cuttings. In this study, shoot cuttings from six selected ornamental woody shrubs were subjected to different concentrations of rooting stimulators (0.5 (NAA5) and 0.8 (NAA8) % concentrations of 1-Naphthylacetic acid; cuttings without treatment were considered as control) and propagated in two different periods (spring and summer). Our results show that significant changes were obtained in the plants under the different treatments. Most of the plants showed a positive response to both treatments, expect for Cotinus coggygria ‘Royal Purple’, which, compared to control, registered decreases in all the tested parameters under NAA5 treatment. Ilex aquifolium was the species that showed increments in all the parameters when NAA treatments were applied. In conclusion, our research suggests that NAA increases rooting in ornamental woody shrubs, although in some cases rooting could be a species-dependent process.

This research was designed to provide the first protocol to establish an efficient solution for direct organogenesis regeneration in Passiflora quadrangularis using nodal explants from young shoots. Passifloraceae tissue culture has been associated with problems such as recalcitrance, sensitivity to ethylene accumulation and browning of explants due to the presence of phenols in the tissues. Due to the high rate of endogenous contamination of the explants, a preliminary experiment was performed. The best results of surface sterilization were obtained using the pretreatment with 70% EtOH, 1 min and 50% NaOCl, 10 min along with the treatment of Rifampicin 15 µg/mL and Benomyl 2 g/L. The effects of plant growth regulators on the induction of direct organogenesis, multiplication of shoots in subcultures and in vitro rooting were evaluated. Additional compounds such as AgNO₃ and Pluronic F-68 were added to the culture media in order to reduce the effects of phenols and the sudden browning of the explants. Shoot proliferation increased to the sixth subculture after which it decreased. A maximum of 7.17 shoots were obtained from one shoot on Murashige and Skoog (MS) medium supplemented with 2 mg/L 6-benzylaminopurine and 1 mg/L thidiazuron. Supplementation of ½ MS medium with 1 mg/L 1-naphthaleneacetic acid was conducing to root formation in 61.11% of shoots. After acclimatization, the plants showed vigorous growth, green leaves, and well-developed roots. Although this species has previously shown difficulty in in vitro propagation, this protocol established based on the results proved to be efficient and reproducible.

This research paper presents a case study analysis of the behavior of three Sedum varieties and their growth in three different types of substrates without additional watering or fertilizing. The study aims to identify a suitable substrate for propagation and to provide insight into the plant’s growth patterns. By analyzing the growth of the Sedum species and varieties—SS’PW’, SS’CB’, and SS’P’—without intervening in their growth process, we were able to identify factors that play a more crucial role in promoting root growth, plant growth, aesthetic value, and use. Over a 20-month period, various technical tools were employed to conduct observations and measurements for both plants and weather conditions. The type of substrate significantly affected plant growth, with the green roof substrate exhibiting the highest overall average monthly root growth rate (0.92 ± 0.05 d, 1.01 ± 0.05 b, 0.96 ± 0.05 c) while in the case of stem growth, among all three varieties, the best results were obtained in the commercial mix (0.87 ± 0.04 a, 0.40 ± 0.02 c, 0.35 ± 0.02 d). Based on the morphological analyses, all values were significantly lower than the control. Best results for leaf weight and surface area were noticed in the green roof substrate with an average growth of 46%, 53%, 55%, and for stem weight, length, and thickness in the commercial mix with 64%, 61%, and 55% compared to the control, respectively. Leaves had varying morphological characteristics, but the chromatic characteristics were preserved. The plants had an overall poor growth which may not be desirable in landscape designs. The findings of this study are applicable in the planning and execution of eco-friendly infrastructure initiatives, leading to the development of more robust and environmentally friendly urban settings.

Geraniums (pelargoniums or storksbills, officially named as Pelargonium zonale (L.) L'Hér. Ex Aiton) are appreciated worldwide due to their ornamental potential and versatility. The research was conducted to emphasize the evolution of morpho-decorative and qualitative traits of two geranium cultivars of high aesthetic value, namely ‘Tango Salmon’ (Montevideo) and ‘Tango Dark Red’, depending on two different fertilization, conventional-chemical fertilization, and unconventional scheme, with a homeopathic solution respectively. It is excessive repetitiveness and is not the case.) cultivar, unconventional fertilization scheme tested conducted to the best results regarding most morpho-decorative characters investigated (33.40 cm height, 21.10 leaves/plant, 34.75 flowers/inflorescence, 2.35% nitrogen, and 48.34 SPAD). For cultivar ‘Tango Dark Red’, the conventional fertilization conducted to the best results in terms of height (18.90 cm), stems/plant (5.90), leaves/plant (14.95), and flowers/inflorescence (37.55), while unconventional fertilization led to higher nitrogen accumulation (2.18%) and enhanced chlorophyll synthesis (47.08 SPAD). Between nitrogen and chlorophyll contents within geranium leaves, strong positive correlations were identified in ‘Tango Salmon’, and weak to moderate in ‘Tango Dark Red’ cultivar. Higher scores were attributed to aesthetic indicators for ‘Tango Salomon’ compared to ‘Tango Dark Red’. The unconventional fertilization of geranium cultivars is an eco-friendly solution for enhancing their morpho-decorative characteristics.

This research investigated the structural and chemical modifications of Dahlia ‘Kennemerland’ across different technological conditions and throughout the vegetation period. Using FT-IR imaging, this study focused on the changes in the inulin, lignin, and suberin contents of tuberous roots. FT-IR maps were generated to visualize the distribution of these compounds across scanned areas, highlighting variations across cultivation methods and seasonal stages. The key compounds analyzed included inulin, lignin, and suberin, which were identified in different root zones. The results showed that inulin was distributed in all analyzed areas, predominantly in zone 1 (periderm), with a distribution that increased with forced cultivation, while lignin and suberin distributions varied with zone and season. Forced tuberous root lignin was detected in all four areas analyzed, in the fall accumulating mainly in area 4 and in suberin starting from summer until autumn. Based on the evaluation of the maps obtained by representing the area ratios of specific bands (inulin/lignin and inulin/suberin), we established where the inulin was present in the highest quantity and concluded that suberin was the constituent with the lowest concentration in tuberous Dahlia roots. These findings emphasize the influence of technological factors and seasonal changes on the biochemical makeup of tuberous Dahlia roots. This detailed biochemical mapping provides insights for optimizing Dahlia cultivation and storage for various industrial applications. This study concludes that FT-IR spectroscopy is an effective tool for monitoring and understanding the biochemical dynamics of Dahlia roots, aiding their agricultural and industrial utilization.