This page has only limited features, please log in for full access.
Sclerotinia sclerotiorum ourmiavirus 17 (SsOV17) was isolated from the hypovirulent strain GF3 of Sclerotinia sclerotiorum. The genome of SsOV17 is 2,802 nt in length and contains a single long open reading frame (ORF) flanked by a short structured 5'-untranslated region (5'-UTR) (28 nt) and a long 3'-UTR (788 nt), respectively. The ORF encodes a protein with 663 amino acids and a predicted molecular mass of 75.0 kDa. A BLASTp search indicated that the protein encoded by SsOV17 is closely related to the putative RNA-dependent RNA polymerase (RdRp) of Sclerotinia sclerotiorum ourmiavirus 13 (71% identity). A multiple sequence alignment indicated that eight conserved amino acid motifs were present in the RdRp conserved region of SsOV17. Phylogenetic analysis demonstrated that SsOV17 clustered with members of the genus Botoulivirus.
Fan Mu; Jichun Jia; Yunxiang Xue; Daohong Jiang; Yanping Fu; Jiasen Cheng; Yang Lin; Jiatao Xie. Characterization of a novel botoulivirus isolated from the phytopathogenic fungus Sclerotinia sclerotiorum. Archives of Virology 2021, 1 .
AMA StyleFan Mu, Jichun Jia, Yunxiang Xue, Daohong Jiang, Yanping Fu, Jiasen Cheng, Yang Lin, Jiatao Xie. Characterization of a novel botoulivirus isolated from the phytopathogenic fungus Sclerotinia sclerotiorum. Archives of Virology. 2021; ():1.
Chicago/Turabian StyleFan Mu; Jichun Jia; Yunxiang Xue; Daohong Jiang; Yanping Fu; Jiasen Cheng; Yang Lin; Jiatao Xie. 2021. "Characterization of a novel botoulivirus isolated from the phytopathogenic fungus Sclerotinia sclerotiorum." Archives of Virology , no. : 1.
The infection by a single-stranded DNA virus, Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1), causes hypovirulence, a reduced growth rate, and other colony morphological changes in its host Sclerotinia sclerotiorum strain DT-8. However, the mechanisms of the decline are still unclear. Using digital RNA sequencing, a transcriptome analysis was conducted to elucidate the phenotype-related genes with expression changes in response to SsHADV-1 infection. A total of 3110 S. sclerotiorum differentially expressed genes (DEGs) were detected during SsHADV-1 infection, 1741 of which were up-regulated, and 1369 were down-regulated. The identified DEGs were involved in several important pathways. DNA replication, DNA damage response, carbohydrate and lipid metabolism, ribosomal assembly, and translation were the affected categories in S. sclerotiorum upon SsHADV-1 infection. Moreover, the infection of SsHADV-1 also suppressed the expression of antiviral RNA silencing and virulence factor genes. These results provide further detailed insights into the effects of SsHADV-1 infection on the whole genome transcription in S. sclerotiorum.
Zheng Qu; Yanping Fu; Yang Lin; Zhenzhen Zhao; Xuekun Zhang; Jiasen Cheng; Jiatao Xie; Tao Chen; Bo Li; Daohong Jiang. Transcriptional Responses of Sclerotinia sclerotiorum to the Infection by SsHADV-1. Journal of Fungi 2021, 7, 493 .
AMA StyleZheng Qu, Yanping Fu, Yang Lin, Zhenzhen Zhao, Xuekun Zhang, Jiasen Cheng, Jiatao Xie, Tao Chen, Bo Li, Daohong Jiang. Transcriptional Responses of Sclerotinia sclerotiorum to the Infection by SsHADV-1. Journal of Fungi. 2021; 7 (7):493.
Chicago/Turabian StyleZheng Qu; Yanping Fu; Yang Lin; Zhenzhen Zhao; Xuekun Zhang; Jiasen Cheng; Jiatao Xie; Tao Chen; Bo Li; Daohong Jiang. 2021. "Transcriptional Responses of Sclerotinia sclerotiorum to the Infection by SsHADV-1." Journal of Fungi 7, no. 7: 493.
Rice sheath blight caused by Rhizoctonia solani is the major disease of rice that seriously threatens food security worldwide. Efficient and eco‐friendly biological approaches are urgently needed since no resistant cultivars are available. In this study, fallow and paddy soils were initially subjected to microbiome analyses, and the results showed that Talaromyces spp. were significantly more abundant in the paddy soil, while Trichoderma spp. were more abundant in the fallow soil, suggesting that Talaromyces spp. could live and survive better in the paddy soil. Five isolates, namely TF‐04, TF‐03, TF‐02, TF‐01, and TA‐02, were isolated from the paddy soil using sclerotia of R. solani as baits and were further evaluated for their activity against rice sheath blight. These isolates efficiently parasitized the hyphae and rotted the sclerotia even at higher water contents in sterilized sand and the soil. Isolate TF‐04 significantly promoted rice growth, reduced the severity of rice sheath blight, and increased rice yield under outdoor conditions. Defense related genes were upregulated and enzyme activities were enhanced in rice treated with isolate TF‐04. Our research supplies a microbiome guided approach to screen biological control agents, provides Talaromyces isolates to biologically control rice sheath blight.
Aqleem Abbas; Yanping Fu; Zheng Qu; Huizhang Zhao; Yongjian Sun; Yang Lin; Jiatao Xie; Jiasen Cheng; Daohong Jiang. Isolation and evaluation of the biocontrol potential of Talaromyces spp. against rice sheath blight guided by soil microbiome. Environmental Microbiology 2021, 1 .
AMA StyleAqleem Abbas, Yanping Fu, Zheng Qu, Huizhang Zhao, Yongjian Sun, Yang Lin, Jiatao Xie, Jiasen Cheng, Daohong Jiang. Isolation and evaluation of the biocontrol potential of Talaromyces spp. against rice sheath blight guided by soil microbiome. Environmental Microbiology. 2021; ():1.
Chicago/Turabian StyleAqleem Abbas; Yanping Fu; Zheng Qu; Huizhang Zhao; Yongjian Sun; Yang Lin; Jiatao Xie; Jiasen Cheng; Daohong Jiang. 2021. "Isolation and evaluation of the biocontrol potential of Talaromyces spp. against rice sheath blight guided by soil microbiome." Environmental Microbiology , no. : 1.
Nidovirales, which accommodates viruses with the largest RNA genomes, includes the notorious coronaviruses; however, the evolutionary route for nidoviruses is not well understood. We have characterized a positive-sense (+) single-stranded (ss) RNA mycovirus, Rhizoctonia solani hypovirus 2 (RsHV2), from the phytopathogenic fungus Rhizoctonia solani. RsHV2 has the largest RNA genome size of 22,219 nucleotides, excluding the poly(A) tail, in all known mycoviruses, and contains two open reading frames (ORF1 and ORF2). ORF1 encodes a 2009 amino acid (aa) protein that includes a conserved helicase domain belonging to helicase superfamily I (SFI). In contrast, ORF2 encodes a 4459 aa polyprotein containing the hallmark genes of hypoviruses. The latter includes a helicase belonging to SFII. Following phylogenetic analysis, the ORF1-encoded helicase (Hel1) unexpectedly clustered in an independent evolutionary branch together with nidovirus helicases, including coronaviruses, and bacteria helicases. Thus, Hel1 presence indicates the occurrence of horizontal gene transfer between viruses and bacteria. These findings also suggest that RsHV2 is most likely a recombinant arising between hypoviruses and nidoviruses.
Assane Hamidou Abdoulaye; Du Hai; Qing Tang; Daohong Jiang; Yanping Fu; Jiasen Cheng; Yang Lin; Bo Li; Ioly Kotta-Loizou; Jiatao Xie. Two distant helicases in one mycovirus: evidence of horizontal gene transfer between mycoviruses, coronaviruses and other nidoviruses. Virus Evolution 2021, 7, veab043 .
AMA StyleAssane Hamidou Abdoulaye, Du Hai, Qing Tang, Daohong Jiang, Yanping Fu, Jiasen Cheng, Yang Lin, Bo Li, Ioly Kotta-Loizou, Jiatao Xie. Two distant helicases in one mycovirus: evidence of horizontal gene transfer between mycoviruses, coronaviruses and other nidoviruses. Virus Evolution. 2021; 7 (1):veab043.
Chicago/Turabian StyleAssane Hamidou Abdoulaye; Du Hai; Qing Tang; Daohong Jiang; Yanping Fu; Jiasen Cheng; Yang Lin; Bo Li; Ioly Kotta-Loizou; Jiatao Xie. 2021. "Two distant helicases in one mycovirus: evidence of horizontal gene transfer between mycoviruses, coronaviruses and other nidoviruses." Virus Evolution 7, no. 1: veab043.
Considering the huge economic loss caused by postharvest diseases, the identification and prevention of citrus postharvest diseases is vital to the citrus industry. In 2018, 16 decayed citrus fruit from four citrus varieties—Satsuma mandarin (Citrus unshiu), Ponkan (Citrus reticulata Blanco cv. Ponkan), Nanfeng mandarin (Citrus reticulata cv. nanfengmiju), and Sugar orange (Citrus reticulata Blanco)—showing soft rot and sogginess on their surfaces and covered with white mycelia were collected from storage rooms in seven provinces. The pathogens were isolated and the pathogenicity of the isolates was tested. The fungal strains were identified as Lasiodiplodia pseudotheobromae based on their morphological characteristics and phylogenetic analyses using the internal transcribed spacer regions (ITS), translation elongation factor 1-α gene (TEF), and beta-tubulin (TUB) gene sequences. The strains could infect wounded citrus fruit and cause decay within two days post inoculation, but could not infect unwounded fruit. To our knowledge, this is the first report of citrus fruit decay caused by L. pseudotheobromae in China.
Jianghua Chen; Zihang Zhu; Yanping Fu; Jiasen Cheng; Jiatao Xie; Yang Lin. Identification of Lasiodiplodia pseudotheobromae Causing Fruit Rot of Citrus in China. Plants 2021, 10, 202 .
AMA StyleJianghua Chen, Zihang Zhu, Yanping Fu, Jiasen Cheng, Jiatao Xie, Yang Lin. Identification of Lasiodiplodia pseudotheobromae Causing Fruit Rot of Citrus in China. Plants. 2021; 10 (2):202.
Chicago/Turabian StyleJianghua Chen; Zihang Zhu; Yanping Fu; Jiasen Cheng; Jiatao Xie; Yang Lin. 2021. "Identification of Lasiodiplodia pseudotheobromae Causing Fruit Rot of Citrus in China." Plants 10, no. 2: 202.
Mycovirus diversity is generally analyzed from isolates of fungal culture isolates at a single point in time as a snapshot. The stability of mycovirus composition within the same geographical location over time remains unclear. Not knowing how the population fluctuates in the field can be a source of unpredictability in the successful application of virocontrol. To better understand the changes over time, we monitored the interannual dynamics and abundance of mycoviruses infecting Sclerotinia sclerotiorum at a rapeseed-growing field for three years. We found that the virome in S. sclerotiorum harbors unique mycovirus compositions each year. In total, sixty-eight mycoviruses were identified, among which twenty-four were detected in all three successive years. These twenty-four mycoviruses can be classified as the members of the core virome in this S. sclerotiorum population, which show persistence and relatively high transmissibility under field conditions. Nearly two-thirds of the mycoviruses have positive-sense, single-stranded RNA genomes and were found consistently across all three years. Moreover, twenty-eight mycoviruses are newly described, including four novel, multi-segmented narnaviruses, and four unique bunyaviruses. Overall, the newly discovered mycoviruses in this study belong to as many as twenty families, into which eight were first identified in S. sclerotiorum, demonstrating evolutionarily diverse viromes. Our findings not only shed light on the annual variation of mycovirus diversity but also provide important virus evolutionary clues.
Jichun Jia; Yanping Fu; Daohong Jiang; Fan Mu; Jiasen Cheng; Yang Lin; Bo Li; Shin-Yi Lee Marzano; Jiatao Xie. Interannual dynamics, diversity and evolution of the virome in Sclerotinia sclerotiorum from a single crop field. Virus Evolution 2021, 7, veab032 .
AMA StyleJichun Jia, Yanping Fu, Daohong Jiang, Fan Mu, Jiasen Cheng, Yang Lin, Bo Li, Shin-Yi Lee Marzano, Jiatao Xie. Interannual dynamics, diversity and evolution of the virome in Sclerotinia sclerotiorum from a single crop field. Virus Evolution. 2021; 7 (1):veab032.
Chicago/Turabian StyleJichun Jia; Yanping Fu; Daohong Jiang; Fan Mu; Jiasen Cheng; Yang Lin; Bo Li; Shin-Yi Lee Marzano; Jiatao Xie. 2021. "Interannual dynamics, diversity and evolution of the virome in Sclerotinia sclerotiorum from a single crop field." Virus Evolution 7, no. 1: veab032.
Via virome sequencing, six viruses were detected from Magnaporthe oryzae strains YC81-2, including one virus in the family Tombusviridae, one virus in the family Narnaviridae and four viruses in the family Botourmiaviridae. Since the RNA-dependent RNA polymerase (RdRp) of one botourmiavirus show the highest identity (79%) with Magnaporthe oryzae ourmia-like virus 1 (MOLV1), the virus that was grouped into the genus Magoulivirus was designated as Magnaporthe oryzae botourmiavirus 2 (MOBV2). The three other novel botourmiaviruses were selected for further study. The complete nucleotide sequences of the three botourmiaviruses were determined. Sequence analysis showed that virus 1, virus 2, and virus 3 were 2598, 2385, and 2326 nts in length, respectively. The variable 3′ untranslated region (3′-UTR) and 5′-UTR of each virus could be folded into a stable stem-loop secondary structure. Each virus consisted of a unique ORF encoding a putative RdRp. The putative proteins with a conserved GDD motif of RdRp showed the highest sequence similarity to RdRps of viruses in the family Botourmiaviridae. Phylogenetic analysis demonstrated that these viruses were three distinct novel botourmiaviruses, clustered into the Botourmiaviridae family but not belonging to any known genera of this family. Thus, virus 1, virus 2, and virus 3 were designated as Magnaporthe oryzae botourmiavirus 5, 6, and 7 (MOBV5, MOBV6, and MOBV7), respectively. Our results suggest that four distinct botourmiaviruses, MOBV2, MOBV5, MOBV6, and MOBV7, co-infect a single strain of Magnaporthe oryzae, and MOBV5, MOBV6, and MOBV7 are members of three unclassified genera in the family Botourmiaviridae.
Yang Liu; Liyan Zhang; Ahmed Esmael; Jie Duan; Xuefeng Bian; Jichun Jia; Jiatao Xie; Jiasen Cheng; Yanping Fu; Daohong Jiang; Yang Lin. Four Novel Botourmiaviruses Co-Infecting an Isolate of the Rice Blast Fungus Magnaporthe oryzae. Viruses 2020, 12, 1383 .
AMA StyleYang Liu, Liyan Zhang, Ahmed Esmael, Jie Duan, Xuefeng Bian, Jichun Jia, Jiatao Xie, Jiasen Cheng, Yanping Fu, Daohong Jiang, Yang Lin. Four Novel Botourmiaviruses Co-Infecting an Isolate of the Rice Blast Fungus Magnaporthe oryzae. Viruses. 2020; 12 (12):1383.
Chicago/Turabian StyleYang Liu; Liyan Zhang; Ahmed Esmael; Jie Duan; Xuefeng Bian; Jichun Jia; Jiatao Xie; Jiasen Cheng; Yanping Fu; Daohong Jiang; Yang Lin. 2020. "Four Novel Botourmiaviruses Co-Infecting an Isolate of the Rice Blast Fungus Magnaporthe oryzae." Viruses 12, no. 12: 1383.
We previously identified Sclerotinia sclerotiorum negative-stranded virus 1 (SsNSRV-1), the first (−) ssRNA mycovirus, associated with hypovirulence of its fungal host Sclerotinia sclerotiorum. In this study, functional analysis of Open Reading Frame Ι (ORF Ι) of SsNSRV-1 was performed. The integration and expression of ORF Ι led to defects in hyphal tips, vegetative growth, and virulence of the mutant strains of S. sclerotiorum. Further, differentially expressed genes (DEGs) responding to the expression of ORF Ι were identified by transcriptome analysis. In all, 686 DEGs consisted of 267 up-regulated genes and 419 down-regulated genes. DEGs reprogramed by ORF Ι were relevant to secretory proteins, pathogenicity, transcription, transmembrane transport, protein biosynthesis, modification, and metabolism. Alternative splicing was also detected in all mutant strains, but not in hypovirulent strain AH98, which was co-infected by SsNSRV-1 and Sclerotinia sclerotiorum hypovirus 1 (SsHV-1). Thus, the integrity of SsNSRV-1 genome may be necessary to protect viral mRNA from splicing and inactivation by the host. Taken together, the results suggested that protein ORF Ι could regulate the transcription, translation, and modification of host genes in order to facilitate viral proliferation and reduce the virulence of the host. Therefore, ORF Ι may be a potential gene used for the prevention of S. sclerotiorum.
Zhixiao Gao; Junyan Wu; Daohong Jiang; Jiatao Xie; Jiasen Cheng; Yang Lin. ORF Ι of Mycovirus SsNSRV-1 is Associated with Debilitating Symptoms of Sclerotinia sclerotiorum. Viruses 2020, 12, 456 .
AMA StyleZhixiao Gao, Junyan Wu, Daohong Jiang, Jiatao Xie, Jiasen Cheng, Yang Lin. ORF Ι of Mycovirus SsNSRV-1 is Associated with Debilitating Symptoms of Sclerotinia sclerotiorum. Viruses. 2020; 12 (4):456.
Chicago/Turabian StyleZhixiao Gao; Junyan Wu; Daohong Jiang; Jiatao Xie; Jiasen Cheng; Yang Lin. 2020. "ORF Ι of Mycovirus SsNSRV-1 is Associated with Debilitating Symptoms of Sclerotinia sclerotiorum." Viruses 12, no. 4: 456.
A novel virus, Botryosphaeria dothidea fusarivirus 1 (BdFV1), was isolated from a fungal strain, SDAU11-86 of Botryosphaeria dothidea, and its complete genome sequence was determined. BdFV1 has a single-stranded positive-sense (+ssRNA) genome with 6,179 nucleotides, excluding the poly(A) tail. The genome of BdFV1 contains two putative open reading frames (ORFs). The first ORF encodes a large polyprotein of 1,544 amino acids (aa) with conserved RNA-dependent RNA polymerase and viral helicase domains. The second ORF encodes a putative 481-aa protein with unknown function. Sequence comparisons and phylogenetic analysis suggested that BdFV1 is a novel mycovirus belonging to the newly proposed family “Fusariviridae”. This is the first report of a +ssRNA mycovirus in B. dothidea.
Wenyi Liu; Du Hai; Fan Mu; Xiaojing Yu; Yingtong Zhao; Bangling He; Jiatao Xie; Daohong Jiang; Huixiang Liu. Molecular characterization of a novel fusarivirus infecting the plant-pathogenic fungus Botryosphaeria dothidea. Archives of Virology 2020, 165, 1033 -1037.
AMA StyleWenyi Liu, Du Hai, Fan Mu, Xiaojing Yu, Yingtong Zhao, Bangling He, Jiatao Xie, Daohong Jiang, Huixiang Liu. Molecular characterization of a novel fusarivirus infecting the plant-pathogenic fungus Botryosphaeria dothidea. Archives of Virology. 2020; 165 (4):1033-1037.
Chicago/Turabian StyleWenyi Liu; Du Hai; Fan Mu; Xiaojing Yu; Yingtong Zhao; Bangling He; Jiatao Xie; Daohong Jiang; Huixiang Liu. 2020. "Molecular characterization of a novel fusarivirus infecting the plant-pathogenic fungus Botryosphaeria dothidea." Archives of Virology 165, no. 4: 1033-1037.
Botrytis cinerea, a ubiquitous necrotrophic plant-pathogenic fungus, is responsible for grey mold and rot disease in a very wide range of plant species. Subtilisin-like proteases (or subtilases) are a very diverse family of serine proteases present in many organisms and are reported to have a broad spectrum of biological functions. Here, we identified two genes encoding subtilisin-like proteases (Bcser1 and Bcser2) in the genome of B. cinerea, both of which contain an inhibitor I9 domain and a peptidase S8 domain. The expression levels of Bcser1 and Bcser2 increased during the sclerotial forming stage, as well as during a later stage of hyphal infection on Arabidopsis thaliana leaves, but the up-regulation of Bcser1 was significantly higher than that of Bcser2. Interestingly, deletion of Bcser1 had no effect on the fungal development or virulence of B. cinerea. However, deletion of Bcser2 or double deletion of Bcser1 and Bcser2 severely impaired the hyphal growth, sclerotial formation and conidiation of B. cinerea. We also found that ∆Bcser2 and ∆Bcser1/2 could not form complete infection cushions and then lost the ability to infect intact plant leaves of Arabidopsis and tomato but could infect wounded plant tissues. Taken together, our results indicate that the subtilisin-like protease Bcser2 is crucial for the sclerotial formation, conidiation, and virulence of B. cinerea.
Xinqiang Liu; Jiatao Xie; Yanping Fu; Daohong Jiang; Tao Chen; Jiasen Cheng. The Subtilisin-Like Protease Bcser2 Affects the Sclerotial Formation, Conidiation and Virulence of Botrytis cinerea. International Journal of Molecular Sciences 2020, 21, 603 .
AMA StyleXinqiang Liu, Jiatao Xie, Yanping Fu, Daohong Jiang, Tao Chen, Jiasen Cheng. The Subtilisin-Like Protease Bcser2 Affects the Sclerotial Formation, Conidiation and Virulence of Botrytis cinerea. International Journal of Molecular Sciences. 2020; 21 (2):603.
Chicago/Turabian StyleXinqiang Liu; Jiatao Xie; Yanping Fu; Daohong Jiang; Tao Chen; Jiasen Cheng. 2020. "The Subtilisin-Like Protease Bcser2 Affects the Sclerotial Formation, Conidiation and Virulence of Botrytis cinerea." International Journal of Molecular Sciences 21, no. 2: 603.
Infection by diverse mycoviruses is a common phenomenon in Sclerotinia sclerotiorum. In this study, the full genome of a single-stranded RNA mycovirus, tentatively named Hubei sclerotinia RNA virus 1 (HuSRV1), was determined in the hypovirulent strain 277 of S. sclerotiorum. The HuSRV1 genome is 4492 nucleotides (nt) long and lacks a poly (A) tail at the 3'- terminus. Sequence analyses showed that the HuSRV1 genome contains four putative open reading frames (ORFs). ORF1a was presumed to encode a protein with a conserved protease domain and a transmembrane domain. This protein is 27% identical to the P2a protein encoded by the subterranean clover mottle virus. ORF1b encodes a protein containing a conserved RNA-dependent RNA polymerase (RdRp) domain, which may be translated into a fusion protein by a -1 ribosome frameshift. This protein is 45.9% identical to P2b encoded by the sowbane mosaic virus. ORF2 was found to encode a putative coat protein, which shares 23% identical to the coat protein encoded by the olive mild mosaic virus. ORF3 was presumed to encode a putative protein with an unknown function. Evolutionary relation analyses indicated that HuSRV1 is related to members within Sobemovirus, but forms a unique phylogenetic branch, suggesting that HuSRV1 represents a new member within Solemoviridae. HuSRV1 virions, approximately 30 nm in diameter, were purified from strain 277. The purified virions were successfully introduced into virulent strain Ep-1PNA367, resulting in a new hypovirulent strain, which confirmed that HuSRV1 confers hypovirulence on S. sclerotiorum.
Ayesha Azhar; Fan Mu; Huang Huang; Jiasen Cheng; Yanping Fu; Muhammad Rizwan Hamid; Daohong Jiang; Jiatao Xie; Mu; Fu; Xie. A Novel RNA Virus Related to Sobemoviruses Confers Hypovirulence on the Phytopathogenic Fungus Sclerotinia sclerotiorum. Viruses 2019, 11, 759 .
AMA StyleAyesha Azhar, Fan Mu, Huang Huang, Jiasen Cheng, Yanping Fu, Muhammad Rizwan Hamid, Daohong Jiang, Jiatao Xie, Mu, Fu, Xie. A Novel RNA Virus Related to Sobemoviruses Confers Hypovirulence on the Phytopathogenic Fungus Sclerotinia sclerotiorum. Viruses. 2019; 11 (8):759.
Chicago/Turabian StyleAyesha Azhar; Fan Mu; Huang Huang; Jiasen Cheng; Yanping Fu; Muhammad Rizwan Hamid; Daohong Jiang; Jiatao Xie; Mu; Fu; Xie. 2019. "A Novel RNA Virus Related to Sobemoviruses Confers Hypovirulence on the Phytopathogenic Fungus Sclerotinia sclerotiorum." Viruses 11, no. 8: 759.
Sclerotinia sclerotiorum, an important phytopathogenic fungus, harbors rich diversity of mycoviruses. Lately, more mycoviruses can be successfully and accurately discovered by deep sequencing, especially those that could not be detected by traditional double-stranded RNA (dsRNA) extraction. Previously, we reported that the hypovirulent S. sclerotiorum strain SZ-150 is coinfected by Sclerotinia sclerotiorum hypovirus 1 (SsHV1) and its related satellite RNA. Here, aside from SsHV1, we detected two other mycoviruses, Sclerotinia sclerotiorum botybirnavirus 3 (SsBV3/SZ-150) and Sclerotinia sclerotiorum mycotymovirus 1 (SsMTV1/SZ-150), coinfecting strain SZ-150, by deep sequencing and assembly of mycovirus-derived small RNAs and determined their full-length genomes. The genome of SsBV3/SZ-150 was found to be composed of two linear dsRNA segments, 6,212, and 5,880 bp in size, respectively. Each dsRNA segment of SsBV3/SZ-150 contains a large open reading frame (ORF) encoding RNA-dependent RNA polymerase (RdRp) and a hypothetical protein. The whole genome of SsBV3/SZ-150 shares more than 95% sequence identity with Botrytis porri botybirnavirus 1 (BpBV1) at the nucleotide (nt) or amino acid level. Thus, SsBV3/SZ-150 was assumed to be a strain of BpBV1. The genome of SsMTV1/SZ-150 consists of 6,391 nt excluding the poly(A) tail. SsMTV1/SZ-150 was predicted to contain a large ORF that encodes a putative replication-associated polyprotein (RP) with three conserved domains of viral RNA methyltransferase, viral RNA helicase, and RdRp. Phylogenetic analyses suggest that SsMTV1/SZ-150 is related, albeit distantly, to members of the family Tymoviridae. Analysis of the small RNAs derived from SsBV3/SZ-150 and SsMTV1/SZ-150 revealed that small-RNA lengths mainly range from 20 to 24 nt, with a peak at 22 nt, and the most abundant 5′-terminal nucleotide is uridine, suggesting that the Dicer 2 and Argonaute 1, two key components in the RNA inference pathway, may play important roles in the resistance to mycoviral infection in S. sclerotiorum. Neither SsBV3/SZ-150 nor SsMTV1/SZ-150 is a causal agent of hypovirulence in strain SZ-150.
Qianqian Wang; Shufen Cheng; Xueqiong Xiao; Jiasen Cheng; Yanping Fu; Tao Chen; Daohong Jiang; Jiatao Xie. Discovery of Two Mycoviruses by High-Throughput Sequencing and Assembly of Mycovirus-Derived Small Silencing RNAs From a Hypovirulent Strain of Sclerotinia sclerotiorum. Frontiers in Microbiology 2019, 10, 1415 .
AMA StyleQianqian Wang, Shufen Cheng, Xueqiong Xiao, Jiasen Cheng, Yanping Fu, Tao Chen, Daohong Jiang, Jiatao Xie. Discovery of Two Mycoviruses by High-Throughput Sequencing and Assembly of Mycovirus-Derived Small Silencing RNAs From a Hypovirulent Strain of Sclerotinia sclerotiorum. Frontiers in Microbiology. 2019; 10 ():1415.
Chicago/Turabian StyleQianqian Wang; Shufen Cheng; Xueqiong Xiao; Jiasen Cheng; Yanping Fu; Tao Chen; Daohong Jiang; Jiatao Xie. 2019. "Discovery of Two Mycoviruses by High-Throughput Sequencing and Assembly of Mycovirus-Derived Small Silencing RNAs From a Hypovirulent Strain of Sclerotinia sclerotiorum." Frontiers in Microbiology 10, no. : 1415.
We previously determined that virions of Sclerotinia sclerotiorum hypovirulence associated DNA virus 1 (SsHADV-1) could directly infect hyphae of Sclerotinia sclerotiorum, resulting in hypovirulence of the fungal host. However, the molecular mechanisms of SsHADV-1 virions disruption of the fungal cell wall barrier and entrance into the host cell are still unclear. To investigate the early response of S. sclerotiorum to SsHADV-1 infection, S. sclerotiorum hyphae were inoculated with purified SsHADV-1 virions. The pre- and post-infection hyphae were collected at one⁻three hours post-inoculation for transcriptome analysis. Further, bioinformatic analysis showed that differentially expressed genes (DEGs) regulated by SsHADV-1 infection were identified in S. sclerotiorum. In total, 187 genes were differentially expressed, consisting of more up-regulated (114) than down-regulated (73) genes. The identified DEGs were involved in several important pathways. Metabolic processes, biosynthesis of antibiotics, and secondary metabolites were the most affected categories in S. sclerotiorum upon SsHADV-1 infection. Cell structure analysis suggested that 26% of the total DEGs were related to membrane tissues. Furthermore, 10 and 27 DEGs were predicted to be located in the cell membrane and mitochondria, respectively. Gene ontology enrichment analyses of the DEGs were performed, followed by functional annotation of the genes. Interestingly, one third of the annotated functional DEGs could be involved in the Ras-small G protein signal transduction pathway. These results revealed that SsHADV-1 virions may be able to bind host membrane proteins and influence signal transduction through Ras-small G protein-coupled receptors during early infection, providing new insight towards the molecular mechanisms of virions infection in S. sclerotiorum.
Feng Ding; Jiasen Cheng; Yanping Fu; Tao Chen; Bo Li; Daohong Jiang; Jiatao Xie. Early Transcriptional Response to DNA Virus Infection in Sclerotinia sclerotiorum. Viruses 2019, 11, 278 .
AMA StyleFeng Ding, Jiasen Cheng, Yanping Fu, Tao Chen, Bo Li, Daohong Jiang, Jiatao Xie. Early Transcriptional Response to DNA Virus Infection in Sclerotinia sclerotiorum. Viruses. 2019; 11 (3):278.
Chicago/Turabian StyleFeng Ding; Jiasen Cheng; Yanping Fu; Tao Chen; Bo Li; Daohong Jiang; Jiatao Xie. 2019. "Early Transcriptional Response to DNA Virus Infection in Sclerotinia sclerotiorum." Viruses 11, no. 3: 278.
Mycovirus infection is a universal phenomenon in the major fungus groups. So far, however, mycoviruses have not been described in the phytopathogenic fungus Leptosphaeria biglobosa, the causal agent of phoma stem canker (blackleg). Here, we report the complete genome sequence of a novel mitovirus, Leptosphaeria biglobosa mitovirus 1 (LbMV1), isolated from the strain H3-38 of L. biglobosa. The LbMV1 genome comprises 2568 nucleotides with a low G+C content of 30%. Using the mitochondrial genetic code, the genome of LbMV1 was found to contain a single large open reading frame that encodes a predicted protein of 756 amino acids. Multiple alignment of the predicted protein sequence showed the highest similarity (51% identity) to the putative RNA-dependent RNA polymerase of Ophiostoma mitovirus 4. In addition, phylogenetic analysis grouped LbMV1 within the genus Mitovirus of the family Narnaviridae. Our results suggest that the double-stranded RNA found in strain H3-38 is the replicating genome of the mitovirus LbMV1, its deletion represents the first evidence of a mycovirus infecting L. biglobosa.
Yong Wang; Zhiyong Xu; Jiasen Cheng; Yanping Fu; Daohong Jiang; Jiatao Xie. Characterization of a novel RNA virus from the phytopathogenic fungus Leptosphaeria biglobosa related to members of the genus Mitovirus. Archives of Virology 2019, 164, 913 -916.
AMA StyleYong Wang, Zhiyong Xu, Jiasen Cheng, Yanping Fu, Daohong Jiang, Jiatao Xie. Characterization of a novel RNA virus from the phytopathogenic fungus Leptosphaeria biglobosa related to members of the genus Mitovirus. Archives of Virology. 2019; 164 (3):913-916.
Chicago/Turabian StyleYong Wang; Zhiyong Xu; Jiasen Cheng; Yanping Fu; Daohong Jiang; Jiatao Xie. 2019. "Characterization of a novel RNA virus from the phytopathogenic fungus Leptosphaeria biglobosa related to members of the genus Mitovirus." Archives of Virology 164, no. 3: 913-916.
Mitogen-activated protein kinase (MAPK) cascades play a central role in cellular growth, proliferation, and survival. MAPK cascade genes have been extensively investigated in model plants, mammals, yeast, and fungi but are not characterized in Plasmodiophora brassicae, which causes clubroot disease in cruciferous plants. Here, we identified 7 PbMAPK, 3 PbMAPKK, and 9 PbMAPKKK genes in the P. brassicae genome. Transcriptional profiling analysis demonstrated that several MAPK, MAPK kinase (MAPKK), and MAPK kinase kinase (MAPKKK) genes were preferentially expressed in three different zoosporic stages. Based on yeast two-hybrid assays, PbMAKKK7 interacted with PbMAKK3 and PbMAKK3 interacted with PbMAK1/PbMAK3. The PbMAKKK7-PbMAKK3-PbMAK1/PbMAK3 cascade may be present in P. brassicae. U0126, a potent and specific inhibitor of MAPKK, could inhibit the germination of P. brassicae resting spores. U0126 was used to treat the resting spores of P. brassicae and coinoculate rapeseed, and was proven to significantly relieve the severity of clubroot symptoms in the host plant and delay the life cycle of P. brassicae. These results suggest that MAPK signaling pathways may play important roles in P. brassicae growth, development, and pathogenicity.
Tao Chen; Kai Bi; Yanli Zhao; Xueliang Lyu; Zhixiao Gao; Ying Zhao; Yanping Fu; Jiasen Cheng; Jiatao Xie; Daohong Jiang. MAPKK Inhibitor U0126 Inhibits Plasmodiophora brassicae Development. Phytopathology® 2018, 108, 711 -720.
AMA StyleTao Chen, Kai Bi, Yanli Zhao, Xueliang Lyu, Zhixiao Gao, Ying Zhao, Yanping Fu, Jiasen Cheng, Jiatao Xie, Daohong Jiang. MAPKK Inhibitor U0126 Inhibits Plasmodiophora brassicae Development. Phytopathology®. 2018; 108 (6):711-720.
Chicago/Turabian StyleTao Chen; Kai Bi; Yanli Zhao; Xueliang Lyu; Zhixiao Gao; Ying Zhao; Yanping Fu; Jiasen Cheng; Jiatao Xie; Daohong Jiang. 2018. "MAPKK Inhibitor U0126 Inhibits Plasmodiophora brassicae Development." Phytopathology® 108, no. 6: 711-720.
Various mycoviruses have been isolated from Sclerotinia sclerotiorum. Here, we identified a viral RNA sequence contig, representing a novel virus, Sclerotinia sclerotiorum deltaflexivirus 2 (SsDFV2), from an RNA_Seq database. We found that SsDFV2 was harbored in the hypovirulent strain, 228, which grew slowly on potato dextrose agar, produced a few sclerotia, and could not induce typical lesions on detached rapeseed (Brassica napus) leaves. Strain 228 was also infected by Botrytis porri RNA Virus 1 (BpRV1), a virus originally isolated from Botrytis porri. The genome of SsDFV2 comprised 6711 nucleotides, excluding the poly (A) tail, and contained a single large predicted open reading frame encoding a putative viral RNA replicase. Phylogenetic analysis demonstrated that SsDFV2 is closely related to viruses in the family Deltaflexiviridae; however, it also differs significantly from members of this family, suggesting that it may represent a new species. Further we determined that SsDFV2 could be efficiently transmitted to host vegetative incompatible individuals by dual culture. To our best knowledge, this is the first report that a (+) ssRNA mycovirus can overcome the transmission limitations of the vegetative incompatibility system, a phenomenon that may facilitate the potential use of mycoviruses for the control of crop fungal diseases.
Muhammad Rizwan Hamid; Jiatao Xie; Songsong Wu; Shahzeen Kanwal Maria; Dan Zheng; Abdoulaye Assane Hamidou; Qihua Wang; Jiasen Cheng; Yanping Fu; Daohong Jiang. A Novel Deltaflexivirus that Infects the Plant Fungal Pathogen, Sclerotinia sclerotiorum, Can Be Transmitted Among Host Vegetative Incompatible Strains. Viruses 2018, 10, 295 .
AMA StyleMuhammad Rizwan Hamid, Jiatao Xie, Songsong Wu, Shahzeen Kanwal Maria, Dan Zheng, Abdoulaye Assane Hamidou, Qihua Wang, Jiasen Cheng, Yanping Fu, Daohong Jiang. A Novel Deltaflexivirus that Infects the Plant Fungal Pathogen, Sclerotinia sclerotiorum, Can Be Transmitted Among Host Vegetative Incompatible Strains. Viruses. 2018; 10 (6):295.
Chicago/Turabian StyleMuhammad Rizwan Hamid; Jiatao Xie; Songsong Wu; Shahzeen Kanwal Maria; Dan Zheng; Abdoulaye Assane Hamidou; Qihua Wang; Jiasen Cheng; Yanping Fu; Daohong Jiang. 2018. "A Novel Deltaflexivirus that Infects the Plant Fungal Pathogen, Sclerotinia sclerotiorum, Can Be Transmitted Among Host Vegetative Incompatible Strains." Viruses 10, no. 6: 295.
The Hypoviridae, comprising one genus, Hypovirus, is a family of capsidless viruses with positive-sense, ssRNA genomes of 9.1-12.7 kb that possess either a single large ORF or two ORFs. The ORFs appear to be translated from genomic RNA by non-canonical mechanisms, i.e. internal ribosome entry site-mediated and stop/restart translation. Hypoviruses have been detected in ascomycetous or basidiomycetous filamentous fungi, and are considered to be replicated in host Golgi-derived, lipid vesicles that contain their dsRNA as a replicative form. Some hypoviruses induce hypovirulence to host fungi, while others do not. This is a summary of the current ICTV report on the taxonomy of the Hypoviridae, which is available at www.ictv.global/report/hypoviridae.
Nobuhiro Suzuki; Said A. Ghabrial; Kook-Hyung Kim; Michael Pearson; Shin-Yi L. Marzano; Hajime Yaegashi; Jiatao Xie; Lihua Guo; Hideki Kondo; Igor Koloniuk; Bradley I. Hillman; ICTV Report Consortium. ICTV Virus Taxonomy Profile: Hypoviridae. Journal of General Virology 2018, 99, 615 -616.
AMA StyleNobuhiro Suzuki, Said A. Ghabrial, Kook-Hyung Kim, Michael Pearson, Shin-Yi L. Marzano, Hajime Yaegashi, Jiatao Xie, Lihua Guo, Hideki Kondo, Igor Koloniuk, Bradley I. Hillman, ICTV Report Consortium. ICTV Virus Taxonomy Profile: Hypoviridae. Journal of General Virology. 2018; 99 (5):615-616.
Chicago/Turabian StyleNobuhiro Suzuki; Said A. Ghabrial; Kook-Hyung Kim; Michael Pearson; Shin-Yi L. Marzano; Hajime Yaegashi; Jiatao Xie; Lihua Guo; Hideki Kondo; Igor Koloniuk; Bradley I. Hillman; ICTV Report Consortium. 2018. "ICTV Virus Taxonomy Profile: Hypoviridae." Journal of General Virology 99, no. 5: 615-616.
Seven dsRNA segments were detected from a single Rhizoctonia solani strain HG81. From the full-length cDNA sequences of four smaller dsRNA segments, the genomes of two related partitiviruses, designated as Rhizoctonia solani partitivirus 3 (RsPV3) and RsPV4, were determined. The genomes of RsPV3 and RsPV4 are both composed of two separate dsRNA segments, with each segment possessing a single open reading frame (ORF). ORF1 from RsPV3 and RsPV4 encodes a putative RNA-dependent RNA polymerase, while ORF2 of RsPV3 and RsPV4 encodes a putative capsid protein. RsPV3 and RsPV4 share high sequence identity with viruses classified within the genus Alphapartitivirus, family Partitiviridae.
Ruiling Lyu; Yi Zhang; Qing Tang; Lyu Ruiling; Jiasen Cheng; Yanping Fu; Tao Chen; Daohong Jiang; Jiatao Xie. Two alphapartitiviruses co-infecting a single isolate of the plant pathogenic fungus Rhizoctonia solani. Archives of Virology 2017, 163, 515 -520.
AMA StyleRuiling Lyu, Yi Zhang, Qing Tang, Lyu Ruiling, Jiasen Cheng, Yanping Fu, Tao Chen, Daohong Jiang, Jiatao Xie. Two alphapartitiviruses co-infecting a single isolate of the plant pathogenic fungus Rhizoctonia solani. Archives of Virology. 2017; 163 (2):515-520.
Chicago/Turabian StyleRuiling Lyu; Yi Zhang; Qing Tang; Lyu Ruiling; Jiasen Cheng; Yanping Fu; Tao Chen; Daohong Jiang; Jiatao Xie. 2017. "Two alphapartitiviruses co-infecting a single isolate of the plant pathogenic fungus Rhizoctonia solani." Archives of Virology 163, no. 2: 515-520.
Cerato-platanin proteins (CPs), which are secreted by filamentous fungi, are phytotoxic to host plants, but their functions have not been well defined to date. Here we characterized a CP (SsCP1) from the necrotrophic phytopathogen Sclerotinia sclerotiorum. Sscp1 transcripts accumulated during plant infection, and deletion of Sscp1 significantly reduced virulence. SsCP1 could induce significant cell death when expressed in Nicotiana benthamiana. Using yeast two-hybrid, GST pull-down, co-immunoprecipitation and bimolecular florescence complementation, we found that SsCP1 interacts with PR1 in the apoplast to facilitate infection by S. sclerotiorum. Overexpressing PR1 enhanced resistance to the wild-type strain, but not to the Sscp1 knockout strain of S. sclerotiorum. Sscp1-expressing transgenic plants showed increased concentrations of salicylic acid (SA) and higher levels of resistance to several plant pathogens (namely Botrytis cinerea, Alternaria brassicicola and Golovinomyces orontii). Our results suggest that SsCP1 is important for virulence of S. sclerotiorum and that it can be recognized by plants to trigger plant defense responses. Our results also suggest that the SA signaling pathway is involved in CP-mediated plant defense .
Guogen Yang; Liguang Tang; Yingdi Gong; Jiatao Xie; Yanping Fu; Daohong Jiang; Tang Liguang; David B. Collinge; Weidong Chen; Jiasen Cheng. A cerato-platanin protein SsCP1 targets plant PR1 and contributes to virulence ofSclerotinia sclerotiorum. New Phytologist 2017, 217, 739 -755.
AMA StyleGuogen Yang, Liguang Tang, Yingdi Gong, Jiatao Xie, Yanping Fu, Daohong Jiang, Tang Liguang, David B. Collinge, Weidong Chen, Jiasen Cheng. A cerato-platanin protein SsCP1 targets plant PR1 and contributes to virulence ofSclerotinia sclerotiorum. New Phytologist. 2017; 217 (2):739-755.
Chicago/Turabian StyleGuogen Yang; Liguang Tang; Yingdi Gong; Jiatao Xie; Yanping Fu; Daohong Jiang; Tang Liguang; David B. Collinge; Weidong Chen; Jiasen Cheng. 2017. "A cerato-platanin protein SsCP1 targets plant PR1 and contributes to virulence ofSclerotinia sclerotiorum." New Phytologist 217, no. 2: 739-755.
We performed molecular cloning and complete genome sequencing of a novel mycoreovirus, Sclerotinia sclerotiorumreovirus 1 (SsReV1), isolated from an isolate of the phytopathogenic fungus Sclerotinia sclerotiorum. SsReV1 has a genome of 28 055 bp and is composed of 11 double-stranded RNA segments. With a combination of unique molecular features, virion shape and composition, and phylogenetic analysis, SsReV1 is significantly distinct from all known reoviruses and defines a novel genus in the family Reoviridae. Interestingly, two conserved domains, double-stranded RNA binding motif (dsRBM, Pfam 00 035) and reovirus sigma C capsid protein (Reo_σC, pfam04 582), were identified in the genome of SsReV1, which are widespread in diverse virus lineages. Sequence comparison and phylogenetic analysis revealed that multiple cross-family horizontal gene transfer (HGT) events could occur between reoviruses and double-stranded DNA viruses, single-stranded RNA viruses and even cellular organisms. Interestingly, the dsRBM of SsReV1 was phylogenetically related to dsRNA-binding proteins of some insects, but not reoviruses. These results indicated that SsReV1 is a new taxonomic representative in Reoviridae, which provides new insights into the diversity and global ecology of reoviruses and other segmented double-stranded RNA viruses. More importantly, the present results provided evidence indicating that reoviruses indeed have HGT events with other virus lineages on a large scale and that HGT may serve as an important driving factor that plays a key role in the evolution of reoviruses.
Lijiang Liu; Jiasen Cheng; Yanping Fu; Huiquan Liu; Daohong Jiang; Jiatao Xie. New insights into reovirus evolution: implications from a newly characterized mycoreovirus. Journal of General Virology 2017, 98, 1132 -1141.
AMA StyleLijiang Liu, Jiasen Cheng, Yanping Fu, Huiquan Liu, Daohong Jiang, Jiatao Xie. New insights into reovirus evolution: implications from a newly characterized mycoreovirus. Journal of General Virology. 2017; 98 (5):1132-1141.
Chicago/Turabian StyleLijiang Liu; Jiasen Cheng; Yanping Fu; Huiquan Liu; Daohong Jiang; Jiatao Xie. 2017. "New insights into reovirus evolution: implications from a newly characterized mycoreovirus." Journal of General Virology 98, no. 5: 1132-1141.