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In March 2021, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by four families (Aliusviridae, Crepuscuviridae, Myriaviridae, and Natareviridae), three subfamilies (Alpharhabdovirinae, Betarhabdovirinae, and Gammarhabdovirinae), 42 genera, and 200 species. Thirty-nine species were renamed and/or moved and seven species were abolished. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV.
Jens H. Kuhn; Scott Adkins; Bernard R. Agwanda; Rim Al Kubrusli; Sergey V. Alkhovsky; Gaya K. Amarasinghe; Tatjana Avšič-Županc; María A. Ayllón; Justin Bahl; Anne Balkema-Buschmann; Matthew J. Ballinger; Christopher F. Basler; Sina Bavari; Martin Beer; Nicolas Bejerman; Andrew J. Bennett; Dennis A. Bente; Éric Bergeron; Brian H. Bird; Carol D. Blair; Kim R. Blasdell; Dag-Ragnar Blystad; Jamie Bojko; Wayne B. Borth; Steven Bradfute; Rachel Breyta; Thomas Briese; Paul A. Brown; Judith K. Brown; Ursula J. Buchholz; Michael J. Buchmeier; Alexander Bukreyev; Felicity Burt; Carmen Büttner; Charles H. Calisher; Mengji Cao; Inmaculada Casas; Kartik Chandran; Rémi N. Charrel; Qi Cheng; Yuya Chiaki; Marco Chiapello; Il-Ryong Choi; Marina Ciuffo; J. Christopher S. Clegg; Ian Crozier; Elena Dal Bó; Juan Carlos de la Torre; Xavier de Lamballerie; Rik L. de Swart; Humberto Debat; Nolwenn M. Dheilly; Emiliano Di Cicco; Nicholas Di Paola; Francesco Di Serio; Ralf G. Dietzgen; Michele Digiaro; Olga Dolnik; Michael A. Drebot; J. Felix Drexler; William G. Dundon; W. Paul Duprex; Ralf Dürrwald; John M. Dye; Andrew J. Easton; Hideki Ebihara; Toufic Elbeaino; Koray Ergünay; Hugh W. Ferguson; Anthony R. Fooks; Marco Forgia; Pierre B. H. Formenty; Jana Fránová; Juliana Freitas-Astúa; Jingjing Fu; Stephanie Fürl; Selma Gago-Zachert; George Fú Gāo; María Laura García; Adolfo García-Sastre; Aura R. Garrison; Thomas Gaskin; Jean-Paul J. Gonzalez; Anthony Griffiths; Tony L. Goldberg; Martin H. Groschup; Stephan Günther; Roy A. Hall; John Hammond; Tong Han; Jussi Hepojoki; Roger Hewson; Jiang Hong; Ni Hong; Seiji Hongo; Masayuki Horie; John S. Hu; Tao Hu; Holly R. Hughes; Florian Hüttner; Timothy H. Hyndman; M. Ilyas; Risto Jalkanen; Dàohóng Jiāng; Gilda B. Jonson; Sandra Junglen; Fujio Kadono; Karia H. Kaukinen; Michael Kawate; Boris Klempa; Jonas Klingström; Gary Kobinger; Igor Koloniuk; Hideki Kondō; Eugene V. Koonin; Mart Krupovic; Kenji Kubota; Gael Kurath; Lies Laenen; Amy J. Lambert; Stanley L. Langevin; Benhur Lee; Elliot J. Lefkowitz; Eric M. Leroy; Shaorong Li; Longhui Li; Jiànróng Lǐ; Huazhen Liu; Igor S. Lukashevich; Piet Maes; William Marciel de Souza; Marco Marklewitz; Sergio H. Marshall; Shin-Yi L. Marzano; Sebastien Massart; John W. McCauley; Michael Melzer; Nicole Mielke-Ehret; Kristina M. Miller; Tobi J. Ming; Ali Mirazimi; Gideon J. Mordecai; Hans-Peter Mühlbach; Elke Mühlberger; Rayapati Naidu; Tomohide Natsuaki; José A. Navarro; Sergey V. Netesov; Gabriele Neumann; Norbert Nowotny; Márcio R. T. Nunes; Alejandro Olmedo-Velarde; Gustavo Palacios; Vicente Pallás; Bernadett Pályi; Anna Papa; Sofia Paraskevopoulou; Adam C. Park; Colin R. Parrish; David A. Patterson; Alex Pauvolid-Corrêa; Janusz T. Pawęska; Susan Payne; Carlotta Peracchio; Daniel R. Pérez; Thomas S. Postler; Liying Qi; Sheli R. Radoshitzky; Renato O. Resende; Carina A. Reyes; Bertus K. Rima; Gabriel Robles Luna; Víctor Romanowski; Paul Rota; Dennis Rubbenstroth; Luisa Rubino; Jonathan A. Runstadler; Sead Sabanadzovic; Amadou Alpha Sall; Maria S. Salvato; Rosemary Sang; Takahide Sasaya; Angela D. Schulze; Martin Schwemmle; Mang Shi; Xiǎohóng Shí; Zhènglì Shí; Yoshifumi Shimomoto; Yukio Shirako; Stuart G. Siddell; Peter Simmonds; Manuela Sironi; Guy Smagghe; Sophie Smither; Jin-Won Song; Kirsten Spann; Jessica R. Spengler; Mark D. Stenglein; David M. Stone; Jari Sugano; Curtis A. Suttle; Amy Tabata; Ayato Takada; Shigeharu Takeuchi; David P. Tchouassi; Amy Teffer; Robert B. Tesh; Natalie J. Thornburg; Yasuhiro Tomitaka; Keizō Tomonaga; Noël Tordo; Baldwyn Torto; Jonathan S. Towner; Shinya Tsuda; Changchun Tu; Massimo Turina; Ioannis E. Tzanetakis; Janice Uchida; Tomio Usugi; Anna Maria Vaira; Marta Vallino; Bernadette Van Den Hoogen; Arvind Varsani; Nikos Vasilakis; Martin Verbeek; Susanne von Bargen; Jiro Wada; Victoria Wahl; Peter J. Walker; Lin-Fa Wang; Guoping Wang; Yanxiang Wang; Yaqin Wang; Muhammad Waqas; Tàiyún Wèi; Shaohua Wen; Anna E. Whitfield; John V. Williams; Yuri I. Wolf; Jiangxiang Wu; Lei Xu; Hironobu Yanagisawa; Caixia Yang; Zuokun Yang; F. Murilo Zerbini; Lifeng Zhai; Yong-Zhen Zhang; Song Zhang; Jinguo Zhang; Zhe Zhang; Xueping Zhou. 2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. Archives of Virology 2021, 1 -54.
AMA StyleJens H. Kuhn, Scott Adkins, Bernard R. Agwanda, Rim Al Kubrusli, Sergey V. Alkhovsky, Gaya K. Amarasinghe, Tatjana Avšič-Županc, María A. Ayllón, Justin Bahl, Anne Balkema-Buschmann, Matthew J. Ballinger, Christopher F. Basler, Sina Bavari, Martin Beer, Nicolas Bejerman, Andrew J. Bennett, Dennis A. Bente, Éric Bergeron, Brian H. Bird, Carol D. Blair, Kim R. Blasdell, Dag-Ragnar Blystad, Jamie Bojko, Wayne B. Borth, Steven Bradfute, Rachel Breyta, Thomas Briese, Paul A. Brown, Judith K. Brown, Ursula J. Buchholz, Michael J. Buchmeier, Alexander Bukreyev, Felicity Burt, Carmen Büttner, Charles H. Calisher, Mengji Cao, Inmaculada Casas, Kartik Chandran, Rémi N. Charrel, Qi Cheng, Yuya Chiaki, Marco Chiapello, Il-Ryong Choi, Marina Ciuffo, J. Christopher S. Clegg, Ian Crozier, Elena Dal Bó, Juan Carlos de la Torre, Xavier de Lamballerie, Rik L. de Swart, Humberto Debat, Nolwenn M. Dheilly, Emiliano Di Cicco, Nicholas Di Paola, Francesco Di Serio, Ralf G. Dietzgen, Michele Digiaro, Olga Dolnik, Michael A. Drebot, J. Felix Drexler, William G. Dundon, W. Paul Duprex, Ralf Dürrwald, John M. Dye, Andrew J. Easton, Hideki Ebihara, Toufic Elbeaino, Koray Ergünay, Hugh W. Ferguson, Anthony R. Fooks, Marco Forgia, Pierre B. H. Formenty, Jana Fránová, Juliana Freitas-Astúa, Jingjing Fu, Stephanie Fürl, Selma Gago-Zachert, George Fú Gāo, María Laura García, Adolfo García-Sastre, Aura R. Garrison, Thomas Gaskin, Jean-Paul J. Gonzalez, Anthony Griffiths, Tony L. Goldberg, Martin H. Groschup, Stephan Günther, Roy A. Hall, John Hammond, Tong Han, Jussi Hepojoki, Roger Hewson, Jiang Hong, Ni Hong, Seiji Hongo, Masayuki Horie, John S. Hu, Tao Hu, Holly R. Hughes, Florian Hüttner, Timothy H. Hyndman, M. Ilyas, Risto Jalkanen, Dàohóng Jiāng, Gilda B. Jonson, Sandra Junglen, Fujio Kadono, Karia H. Kaukinen, Michael Kawate, Boris Klempa, Jonas Klingström, Gary Kobinger, Igor Koloniuk, Hideki Kondō, Eugene V. Koonin, Mart Krupovic, Kenji Kubota, Gael Kurath, Lies Laenen, Amy J. Lambert, Stanley L. Langevin, Benhur Lee, Elliot J. Lefkowitz, Eric M. Leroy, Shaorong Li, Longhui Li, Jiànróng Lǐ, Huazhen Liu, Igor S. Lukashevich, Piet Maes, William Marciel de Souza, Marco Marklewitz, Sergio H. Marshall, Shin-Yi L. Marzano, Sebastien Massart, John W. McCauley, Michael Melzer, Nicole Mielke-Ehret, Kristina M. Miller, Tobi J. Ming, Ali Mirazimi, Gideon J. Mordecai, Hans-Peter Mühlbach, Elke Mühlberger, Rayapati Naidu, Tomohide Natsuaki, José A. Navarro, Sergey V. Netesov, Gabriele Neumann, Norbert Nowotny, Márcio R. T. Nunes, Alejandro Olmedo-Velarde, Gustavo Palacios, Vicente Pallás, Bernadett Pályi, Anna Papa, Sofia Paraskevopoulou, Adam C. Park, Colin R. Parrish, David A. Patterson, Alex Pauvolid-Corrêa, Janusz T. Pawęska, Susan Payne, Carlotta Peracchio, Daniel R. Pérez, Thomas S. Postler, Liying Qi, Sheli R. Radoshitzky, Renato O. Resende, Carina A. Reyes, Bertus K. Rima, Gabriel Robles Luna, Víctor Romanowski, Paul Rota, Dennis Rubbenstroth, Luisa Rubino, Jonathan A. Runstadler, Sead Sabanadzovic, Amadou Alpha Sall, Maria S. Salvato, Rosemary Sang, Takahide Sasaya, Angela D. Schulze, Martin Schwemmle, Mang Shi, Xiǎohóng Shí, Zhènglì Shí, Yoshifumi Shimomoto, Yukio Shirako, Stuart G. Siddell, Peter Simmonds, Manuela Sironi, Guy Smagghe, Sophie Smither, Jin-Won Song, Kirsten Spann, Jessica R. Spengler, Mark D. Stenglein, David M. Stone, Jari Sugano, Curtis A. Suttle, Amy Tabata, Ayato Takada, Shigeharu Takeuchi, David P. Tchouassi, Amy Teffer, Robert B. Tesh, Natalie J. Thornburg, Yasuhiro Tomitaka, Keizō Tomonaga, Noël Tordo, Baldwyn Torto, Jonathan S. Towner, Shinya Tsuda, Changchun Tu, Massimo Turina, Ioannis E. Tzanetakis, Janice Uchida, Tomio Usugi, Anna Maria Vaira, Marta Vallino, Bernadette Van Den Hoogen, Arvind Varsani, Nikos Vasilakis, Martin Verbeek, Susanne von Bargen, Jiro Wada, Victoria Wahl, Peter J. Walker, Lin-Fa Wang, Guoping Wang, Yanxiang Wang, Yaqin Wang, Muhammad Waqas, Tàiyún Wèi, Shaohua Wen, Anna E. Whitfield, John V. Williams, Yuri I. Wolf, Jiangxiang Wu, Lei Xu, Hironobu Yanagisawa, Caixia Yang, Zuokun Yang, F. Murilo Zerbini, Lifeng Zhai, Yong-Zhen Zhang, Song Zhang, Jinguo Zhang, Zhe Zhang, Xueping Zhou. 2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. Archives of Virology. 2021; ():1-54.
Chicago/Turabian StyleJens H. Kuhn; Scott Adkins; Bernard R. Agwanda; Rim Al Kubrusli; Sergey V. Alkhovsky; Gaya K. Amarasinghe; Tatjana Avšič-Županc; María A. Ayllón; Justin Bahl; Anne Balkema-Buschmann; Matthew J. Ballinger; Christopher F. Basler; Sina Bavari; Martin Beer; Nicolas Bejerman; Andrew J. Bennett; Dennis A. Bente; Éric Bergeron; Brian H. Bird; Carol D. Blair; Kim R. Blasdell; Dag-Ragnar Blystad; Jamie Bojko; Wayne B. Borth; Steven Bradfute; Rachel Breyta; Thomas Briese; Paul A. Brown; Judith K. Brown; Ursula J. Buchholz; Michael J. Buchmeier; Alexander Bukreyev; Felicity Burt; Carmen Büttner; Charles H. Calisher; Mengji Cao; Inmaculada Casas; Kartik Chandran; Rémi N. Charrel; Qi Cheng; Yuya Chiaki; Marco Chiapello; Il-Ryong Choi; Marina Ciuffo; J. Christopher S. Clegg; Ian Crozier; Elena Dal Bó; Juan Carlos de la Torre; Xavier de Lamballerie; Rik L. de Swart; Humberto Debat; Nolwenn M. Dheilly; Emiliano Di Cicco; Nicholas Di Paola; Francesco Di Serio; Ralf G. Dietzgen; Michele Digiaro; Olga Dolnik; Michael A. Drebot; J. Felix Drexler; William G. Dundon; W. Paul Duprex; Ralf Dürrwald; John M. Dye; Andrew J. Easton; Hideki Ebihara; Toufic Elbeaino; Koray Ergünay; Hugh W. Ferguson; Anthony R. Fooks; Marco Forgia; Pierre B. H. Formenty; Jana Fránová; Juliana Freitas-Astúa; Jingjing Fu; Stephanie Fürl; Selma Gago-Zachert; George Fú Gāo; María Laura García; Adolfo García-Sastre; Aura R. Garrison; Thomas Gaskin; Jean-Paul J. Gonzalez; Anthony Griffiths; Tony L. Goldberg; Martin H. Groschup; Stephan Günther; Roy A. Hall; John Hammond; Tong Han; Jussi Hepojoki; Roger Hewson; Jiang Hong; Ni Hong; Seiji Hongo; Masayuki Horie; John S. Hu; Tao Hu; Holly R. Hughes; Florian Hüttner; Timothy H. Hyndman; M. Ilyas; Risto Jalkanen; Dàohóng Jiāng; Gilda B. Jonson; Sandra Junglen; Fujio Kadono; Karia H. Kaukinen; Michael Kawate; Boris Klempa; Jonas Klingström; Gary Kobinger; Igor Koloniuk; Hideki Kondō; Eugene V. Koonin; Mart Krupovic; Kenji Kubota; Gael Kurath; Lies Laenen; Amy J. Lambert; Stanley L. Langevin; Benhur Lee; Elliot J. Lefkowitz; Eric M. Leroy; Shaorong Li; Longhui Li; Jiànróng Lǐ; Huazhen Liu; Igor S. Lukashevich; Piet Maes; William Marciel de Souza; Marco Marklewitz; Sergio H. Marshall; Shin-Yi L. Marzano; Sebastien Massart; John W. McCauley; Michael Melzer; Nicole Mielke-Ehret; Kristina M. Miller; Tobi J. Ming; Ali Mirazimi; Gideon J. Mordecai; Hans-Peter Mühlbach; Elke Mühlberger; Rayapati Naidu; Tomohide Natsuaki; José A. Navarro; Sergey V. Netesov; Gabriele Neumann; Norbert Nowotny; Márcio R. T. Nunes; Alejandro Olmedo-Velarde; Gustavo Palacios; Vicente Pallás; Bernadett Pályi; Anna Papa; Sofia Paraskevopoulou; Adam C. Park; Colin R. Parrish; David A. Patterson; Alex Pauvolid-Corrêa; Janusz T. Pawęska; Susan Payne; Carlotta Peracchio; Daniel R. Pérez; Thomas S. Postler; Liying Qi; Sheli R. Radoshitzky; Renato O. Resende; Carina A. Reyes; Bertus K. Rima; Gabriel Robles Luna; Víctor Romanowski; Paul Rota; Dennis Rubbenstroth; Luisa Rubino; Jonathan A. Runstadler; Sead Sabanadzovic; Amadou Alpha Sall; Maria S. Salvato; Rosemary Sang; Takahide Sasaya; Angela D. Schulze; Martin Schwemmle; Mang Shi; Xiǎohóng Shí; Zhènglì Shí; Yoshifumi Shimomoto; Yukio Shirako; Stuart G. Siddell; Peter Simmonds; Manuela Sironi; Guy Smagghe; Sophie Smither; Jin-Won Song; Kirsten Spann; Jessica R. Spengler; Mark D. Stenglein; David M. Stone; Jari Sugano; Curtis A. Suttle; Amy Tabata; Ayato Takada; Shigeharu Takeuchi; David P. Tchouassi; Amy Teffer; Robert B. Tesh; Natalie J. Thornburg; Yasuhiro Tomitaka; Keizō Tomonaga; Noël Tordo; Baldwyn Torto; Jonathan S. Towner; Shinya Tsuda; Changchun Tu; Massimo Turina; Ioannis E. Tzanetakis; Janice Uchida; Tomio Usugi; Anna Maria Vaira; Marta Vallino; Bernadette Van Den Hoogen; Arvind Varsani; Nikos Vasilakis; Martin Verbeek; Susanne von Bargen; Jiro Wada; Victoria Wahl; Peter J. Walker; Lin-Fa Wang; Guoping Wang; Yanxiang Wang; Yaqin Wang; Muhammad Waqas; Tàiyún Wèi; Shaohua Wen; Anna E. Whitfield; John V. Williams; Yuri I. Wolf; Jiangxiang Wu; Lei Xu; Hironobu Yanagisawa; Caixia Yang; Zuokun Yang; F. Murilo Zerbini; Lifeng Zhai; Yong-Zhen Zhang; Song Zhang; Jinguo Zhang; Zhe Zhang; Xueping Zhou. 2021. "2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales." Archives of Virology , no. : 1-54.
Sclerotinia sclerotiorum and Botrytis cinerea are typical necrotrophic pathogens that can attack more than 700 and 3000 plant species, respectively, and cause huge economic losses across numerous crops. In particular, the absence of resistant cultivars makes the stem rot because of S. sclerotiorum the major threat of rapeseed (Brassica napus) worldwide along with Botrytis. Previously, we identified an effector-like protein (SsSSVP1) from S. sclerotiorum and a homologue of SsSSVP1 on B. cinerea genome and found that SsSSVP1 could interact with BnQCR8 of rapeseed, a subunit of the cytochrome b-c1 complex. In this study, we found that BnQCR8 has eight homologous copies in rapeseed cultivar Westar and reduced the copy number of BnQCR8 using CRISPR/Cas9 to improve rapeseed resistance against S. sclerotiorum. Mutants with one or more copies of BnQCR8 edited showed strong resistance against S. sclerotiorum and B. cinerea. BnQCR8-edited mutants did not show significant difference from Westar in terms of respiration and agronomic traits tested, including the plant shape, flowering time, silique size, seed number, thousand seed weight and seed oil content. These traits make it possible to use these mutants directly for commercial production. Our study highlights a common gene for breeding of rapeseed to unravel the key hindrance of rapeseed production caused by S. sclerotiorum and B. cinerea. In contrast to previously established methodologies, our findings provide a novel strategy to develop crops with high resistance against multiple pathogens by editing only a single gene that encodes the common target of pathogen effectors.
Xuekun Zhang; Jiasen Cheng; Yang Lin; Yanping Fu; Jiatao Xie; Bo Li; Xuefeng Bian; Yanbo Feng; Weibo Liang; Qian Tang; Hongxiang Zhang; Xiaofan Liu; Yu Zhang; Changxing Liu; Daohong Jiang. Editing homologous copies of an essential gene affords crop resistance against two cosmopolitan necrotrophic pathogens. Plant Biotechnology Journal 2021, 1 .
AMA StyleXuekun Zhang, Jiasen Cheng, Yang Lin, Yanping Fu, Jiatao Xie, Bo Li, Xuefeng Bian, Yanbo Feng, Weibo Liang, Qian Tang, Hongxiang Zhang, Xiaofan Liu, Yu Zhang, Changxing Liu, Daohong Jiang. Editing homologous copies of an essential gene affords crop resistance against two cosmopolitan necrotrophic pathogens. Plant Biotechnology Journal. 2021; ():1.
Chicago/Turabian StyleXuekun Zhang; Jiasen Cheng; Yang Lin; Yanping Fu; Jiatao Xie; Bo Li; Xuefeng Bian; Yanbo Feng; Weibo Liang; Qian Tang; Hongxiang Zhang; Xiaofan Liu; Yu Zhang; Changxing Liu; Daohong Jiang. 2021. "Editing homologous copies of an essential gene affords crop resistance against two cosmopolitan necrotrophic pathogens." Plant Biotechnology Journal , no. : 1.
The infection by a single-stranded DNA virus, Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1), causes hypovirulence, a reduced growth rate, and other colony morphological changes in its host Sclerotinia sclerotiorum strain DT-8. However, the mechanisms of the decline are still unclear. Using digital RNA sequencing, a transcriptome analysis was conducted to elucidate the phenotype-related genes with expression changes in response to SsHADV-1 infection. A total of 3110 S. sclerotiorum differentially expressed genes (DEGs) were detected during SsHADV-1 infection, 1741 of which were up-regulated, and 1369 were down-regulated. The identified DEGs were involved in several important pathways. DNA replication, DNA damage response, carbohydrate and lipid metabolism, ribosomal assembly, and translation were the affected categories in S. sclerotiorum upon SsHADV-1 infection. Moreover, the infection of SsHADV-1 also suppressed the expression of antiviral RNA silencing and virulence factor genes. These results provide further detailed insights into the effects of SsHADV-1 infection on the whole genome transcription in S. sclerotiorum.
Zheng Qu; Yanping Fu; Yang Lin; Zhenzhen Zhao; Xuekun Zhang; Jiasen Cheng; Jiatao Xie; Tao Chen; Bo Li; Daohong Jiang. Transcriptional Responses of Sclerotinia sclerotiorum to the Infection by SsHADV-1. Journal of Fungi 2021, 7, 493 .
AMA StyleZheng Qu, Yanping Fu, Yang Lin, Zhenzhen Zhao, Xuekun Zhang, Jiasen Cheng, Jiatao Xie, Tao Chen, Bo Li, Daohong Jiang. Transcriptional Responses of Sclerotinia sclerotiorum to the Infection by SsHADV-1. Journal of Fungi. 2021; 7 (7):493.
Chicago/Turabian StyleZheng Qu; Yanping Fu; Yang Lin; Zhenzhen Zhao; Xuekun Zhang; Jiasen Cheng; Jiatao Xie; Tao Chen; Bo Li; Daohong Jiang. 2021. "Transcriptional Responses of Sclerotinia sclerotiorum to the Infection by SsHADV-1." Journal of Fungi 7, no. 7: 493.
Rice sheath blight caused by Rhizoctonia solani is the major disease of rice that seriously threatens food security worldwide. Efficient and eco‐friendly biological approaches are urgently needed since no resistant cultivars are available. In this study, fallow and paddy soils were initially subjected to microbiome analyses, and the results showed that Talaromyces spp. were significantly more abundant in the paddy soil, while Trichoderma spp. were more abundant in the fallow soil, suggesting that Talaromyces spp. could live and survive better in the paddy soil. Five isolates, namely TF‐04, TF‐03, TF‐02, TF‐01, and TA‐02, were isolated from the paddy soil using sclerotia of R. solani as baits and were further evaluated for their activity against rice sheath blight. These isolates efficiently parasitized the hyphae and rotted the sclerotia even at higher water contents in sterilized sand and the soil. Isolate TF‐04 significantly promoted rice growth, reduced the severity of rice sheath blight, and increased rice yield under outdoor conditions. Defense related genes were upregulated and enzyme activities were enhanced in rice treated with isolate TF‐04. Our research supplies a microbiome guided approach to screen biological control agents, provides Talaromyces isolates to biologically control rice sheath blight.
Aqleem Abbas; Yanping Fu; Zheng Qu; Huizhang Zhao; Yongjian Sun; Yang Lin; Jiatao Xie; Jiasen Cheng; Daohong Jiang. Isolation and evaluation of the biocontrol potential of Talaromyces spp. against rice sheath blight guided by soil microbiome. Environmental Microbiology 2021, 1 .
AMA StyleAqleem Abbas, Yanping Fu, Zheng Qu, Huizhang Zhao, Yongjian Sun, Yang Lin, Jiatao Xie, Jiasen Cheng, Daohong Jiang. Isolation and evaluation of the biocontrol potential of Talaromyces spp. against rice sheath blight guided by soil microbiome. Environmental Microbiology. 2021; ():1.
Chicago/Turabian StyleAqleem Abbas; Yanping Fu; Zheng Qu; Huizhang Zhao; Yongjian Sun; Yang Lin; Jiatao Xie; Jiasen Cheng; Daohong Jiang. 2021. "Isolation and evaluation of the biocontrol potential of Talaromyces spp. against rice sheath blight guided by soil microbiome." Environmental Microbiology , no. : 1.
Eggplant (Solanum melongena L.), which belongs to the Solanaceae family, is an important vegetable crop. However, its production is severely threatened by root-knot nematodes (RKNs) in many countries. Solanum torvum, a wild relative of eggplant, is employed worldwide as rootstock for eggplant cultivation due to its resistance to soil-borne diseases such as RKNs. In this study, to identify the RKN defense mechanisms, the transcriptomic profiles of eggplant and Solanum torvum were compared. A total of 5360 differentially expressed genes (DEGs) were identified for the response to RKN infection. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that these DEGs are mainly involved in the processes of response to stimulus, protein phosphorylation, hormone signal transduction, and plant-pathogen interaction pathways. Many phytohormone-related genes and transcription factors (MYB, WRKY, and NAC) were differentially expressed at the four time points (ck, 7, 14, and 28 days post-infection). The abscisic acid signaling pathway might be involved in plant-nematode interactions. qRT-PCR validated the expression levels of some of the DEGs in eggplant. These findings demonstrate the nematode-induced expression profiles and provide some insights into the nematode resistance mechanism in eggplant.
Min Zhang; Hongyuan Zhang; Jie Tan; Shuping Huang; Xia Chen; Daohong Jiang; Xueqiong Xiao. Transcriptome Analysis of Eggplant Root in Response to Root-Knot Nematode Infection. Pathogens 2021, 10, 470 .
AMA StyleMin Zhang, Hongyuan Zhang, Jie Tan, Shuping Huang, Xia Chen, Daohong Jiang, Xueqiong Xiao. Transcriptome Analysis of Eggplant Root in Response to Root-Knot Nematode Infection. Pathogens. 2021; 10 (4):470.
Chicago/Turabian StyleMin Zhang; Hongyuan Zhang; Jie Tan; Shuping Huang; Xia Chen; Daohong Jiang; Xueqiong Xiao. 2021. "Transcriptome Analysis of Eggplant Root in Response to Root-Knot Nematode Infection." Pathogens 10, no. 4: 470.
Fusarium graminearum is an important worldwide pathogen that causes Fusarium head blight (FHB) in wheat, barley, maize, and other grains. LncRNAs play important roles in many biological processes, but little is known about their functions and mechanisms in filamentous fungi. Here, we report that a natural antisense RNA, GzmetE‐AS, is transcribed from the opposite strand of GzmetE. GzmetE encodes a homoserine O‐acetyltransferase, which is important for sexual development and plant infection. The expression of GzmetE‐AS was increased significantly during the conidiation stage, while GzmetE was upregulated in the late stage of sexual reproduction. Overexpression of GzmetE‐AS inhibited the transcription of GzmetE. In contrast, the expression of GzmetE was significantly increased in GzmetE‐AS transcription termination strain GzmetE‐AS‐T. Furthermore, GzmetE‐AS‐T produced more perithecia and facilitated the ascospore discharge, resembling the phenotype of GzmetE overexpressing strains. However, overexpression of GzmetE‐AS in ∆dcl1/2 strain cannot inhibit the expression of GzmetE, and the GzmetE nat‐siRNA is also significantly reduced in ∆dcl1/2 mutant. Taken together, we have identified a novel antisense lncRNA GzmetE‐AS, which is involved in asexual and sexual reproduction by regulating its antisense gene GzmetE through RNAi pathway. Our findings reveal that the lncRNA plays critical roles in the development in F. graminearum.
Jie Wang; Wenping Zeng; Jiatao Xie; Yanping Fu; Daohong Jiang; Yang Lin; Weidong Chen; Jiasen Cheng. A novel antisense long non‐coding RNA participates in asexual and sexual reproduction by regulating the expression of GzmetE in Fusarium graminearum. Environmental Microbiology 2021, 1 .
AMA StyleJie Wang, Wenping Zeng, Jiatao Xie, Yanping Fu, Daohong Jiang, Yang Lin, Weidong Chen, Jiasen Cheng. A novel antisense long non‐coding RNA participates in asexual and sexual reproduction by regulating the expression of GzmetE in Fusarium graminearum. Environmental Microbiology. 2021; ():1.
Chicago/Turabian StyleJie Wang; Wenping Zeng; Jiatao Xie; Yanping Fu; Daohong Jiang; Yang Lin; Weidong Chen; Jiasen Cheng. 2021. "A novel antisense long non‐coding RNA participates in asexual and sexual reproduction by regulating the expression of GzmetE in Fusarium graminearum." Environmental Microbiology , no. : 1.
Via virome sequencing, six viruses were detected from Magnaporthe oryzae strains YC81-2, including one virus in the family Tombusviridae, one virus in the family Narnaviridae and four viruses in the family Botourmiaviridae. Since the RNA-dependent RNA polymerase (RdRp) of one botourmiavirus show the highest identity (79%) with Magnaporthe oryzae ourmia-like virus 1 (MOLV1), the virus that was grouped into the genus Magoulivirus was designated as Magnaporthe oryzae botourmiavirus 2 (MOBV2). The three other novel botourmiaviruses were selected for further study. The complete nucleotide sequences of the three botourmiaviruses were determined. Sequence analysis showed that virus 1, virus 2, and virus 3 were 2598, 2385, and 2326 nts in length, respectively. The variable 3′ untranslated region (3′-UTR) and 5′-UTR of each virus could be folded into a stable stem-loop secondary structure. Each virus consisted of a unique ORF encoding a putative RdRp. The putative proteins with a conserved GDD motif of RdRp showed the highest sequence similarity to RdRps of viruses in the family Botourmiaviridae. Phylogenetic analysis demonstrated that these viruses were three distinct novel botourmiaviruses, clustered into the Botourmiaviridae family but not belonging to any known genera of this family. Thus, virus 1, virus 2, and virus 3 were designated as Magnaporthe oryzae botourmiavirus 5, 6, and 7 (MOBV5, MOBV6, and MOBV7), respectively. Our results suggest that four distinct botourmiaviruses, MOBV2, MOBV5, MOBV6, and MOBV7, co-infect a single strain of Magnaporthe oryzae, and MOBV5, MOBV6, and MOBV7 are members of three unclassified genera in the family Botourmiaviridae.
Yang Liu; Liyan Zhang; Ahmed Esmael; Jie Duan; Xuefeng Bian; Jichun Jia; Jiatao Xie; Jiasen Cheng; Yanping Fu; Daohong Jiang; Yang Lin. Four Novel Botourmiaviruses Co-Infecting an Isolate of the Rice Blast Fungus Magnaporthe oryzae. Viruses 2020, 12, 1383 .
AMA StyleYang Liu, Liyan Zhang, Ahmed Esmael, Jie Duan, Xuefeng Bian, Jichun Jia, Jiatao Xie, Jiasen Cheng, Yanping Fu, Daohong Jiang, Yang Lin. Four Novel Botourmiaviruses Co-Infecting an Isolate of the Rice Blast Fungus Magnaporthe oryzae. Viruses. 2020; 12 (12):1383.
Chicago/Turabian StyleYang Liu; Liyan Zhang; Ahmed Esmael; Jie Duan; Xuefeng Bian; Jichun Jia; Jiatao Xie; Jiasen Cheng; Yanping Fu; Daohong Jiang; Yang Lin. 2020. "Four Novel Botourmiaviruses Co-Infecting an Isolate of the Rice Blast Fungus Magnaporthe oryzae." Viruses 12, no. 12: 1383.
Plant disease is one of the most important causes of crop losses worldwide. The effective control of plant disease is related to food security. Sclerotinia stem rot (SSR) caused by Sclerotinia sclerotiorum leads to serious yield losses in rapeseed (Brassica napus) production. Hypovirulent strain DT-8 of S. sclerotiorum, infected with Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1), has the potential to control SSR. In this study, we found rapeseed bio-priming with strain DT-8 could significantly decrease the disease severity of SSR and increase yield in the field. After bio-priming, strain DT-8 could be detected on the aerial part of the rapeseed plant. By 16S rRNA gene and internal transcribed spacer (ITS) sequencing technique, the microbiome on different parts of the SSR lesion on bioprimed and non-bioprimed rapeseed stem was determined. The results indicated that SSR and bio-priming treatment could influence the structure and composition of fungal and bacterial communities. Bio-priming treatment could reduce the total abundance of possible plant pathogens and enhance the connectivity and robustness of the interaction network at the genus level. This might be one of the mechanisms that rapeseed bioprimed with strain DT-8 had excellent tolerance on SSR. It might be another possible mechanism of biocontrol and will provide a theoretical guide for agricultural practical production.
Zheng Qu; Huizhang Zhao; Hongxiang Zhang; Qianqian Wang; Yao Yao; Jiasen Cheng; Yang Lin; Jiatao Xie; Yanping Fu; Daohong Jiang. Bio-priming with a hypovirulent phytopathogenic fungus enhances the connection and strength of microbial interaction network in rapeseed. npj Biofilms and Microbiomes 2020, 6, 1 -13.
AMA StyleZheng Qu, Huizhang Zhao, Hongxiang Zhang, Qianqian Wang, Yao Yao, Jiasen Cheng, Yang Lin, Jiatao Xie, Yanping Fu, Daohong Jiang. Bio-priming with a hypovirulent phytopathogenic fungus enhances the connection and strength of microbial interaction network in rapeseed. npj Biofilms and Microbiomes. 2020; 6 (1):1-13.
Chicago/Turabian StyleZheng Qu; Huizhang Zhao; Hongxiang Zhang; Qianqian Wang; Yao Yao; Jiasen Cheng; Yang Lin; Jiatao Xie; Yanping Fu; Daohong Jiang. 2020. "Bio-priming with a hypovirulent phytopathogenic fungus enhances the connection and strength of microbial interaction network in rapeseed." npj Biofilms and Microbiomes 6, no. 1: 1-13.
Mycoviruses are viruses that infect fungi, and hypovirulence-associated mycoviruses have the potential to control fungal diseases. However, it is unclear how mycovirus-mediated hypovirulent strains live and survive in the field, and no mycovirus has been applied for field crop protection. In this study, we found that a previously identified small DNA mycovirus (SsHADV-1) can convert its host, Sclerotinia sclerotiorum, from a typical necrotrophic pathogen to a beneficial endophytic fungus. SsHADV-1 downregulates the expression of key pathogenicity factor genes in S. sclerotiorum during infection. When growing in rapeseed, the SsHADV-1-infected strain DT-8 significantly regulates the expression of rapeseed genes involved in defense, hormone signaling, and circadian rhythm pathways. As a result, plant growth is promoted and disease resistance is enhanced. Field experiments showed that spraying DT-8 at the early flowering stage can reduce the disease severity of rapeseed stem rot by 67.6% and improve yield by 14.9%. Moreover, we discovered that SsHADV-1 could also infect other S. sclerotiorum strains on DT-8-inoculated plants and that DT-8 could be recovered from dead plants. These findings suggest that the mycoviruses may have the ability to shape the origin of endophytism. Our discoveries suggest that mycoviruses may influence the origin of endophytism and may also offer a novel strategy for disease control in which mycovirus-infected strains are used to improve crop health and release mycoviruses into the field.
Hongxiang Zhang; Jiatao Xie; Yanping Fu; Jiasen Cheng; Zheng Qu; Zhenzhen Zhao; Shufen Cheng; Tao Chen; Bo Li; Qianqian Wang; Xinqiang Liu; Binnian Tian; David B. Collinge; Daohong Jiang. A 2-kb Mycovirus Converts a Pathogenic Fungus into a Beneficial Endophyte for Brassica Protection and Yield Enhancement. Molecular Plant 2020, 13, 1420 -1433.
AMA StyleHongxiang Zhang, Jiatao Xie, Yanping Fu, Jiasen Cheng, Zheng Qu, Zhenzhen Zhao, Shufen Cheng, Tao Chen, Bo Li, Qianqian Wang, Xinqiang Liu, Binnian Tian, David B. Collinge, Daohong Jiang. A 2-kb Mycovirus Converts a Pathogenic Fungus into a Beneficial Endophyte for Brassica Protection and Yield Enhancement. Molecular Plant. 2020; 13 (10):1420-1433.
Chicago/Turabian StyleHongxiang Zhang; Jiatao Xie; Yanping Fu; Jiasen Cheng; Zheng Qu; Zhenzhen Zhao; Shufen Cheng; Tao Chen; Bo Li; Qianqian Wang; Xinqiang Liu; Binnian Tian; David B. Collinge; Daohong Jiang. 2020. "A 2-kb Mycovirus Converts a Pathogenic Fungus into a Beneficial Endophyte for Brassica Protection and Yield Enhancement." Molecular Plant 13, no. 10: 1420-1433.
In March 2020, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. At the genus rank, 20 new genera were added, two were deleted, one was moved, and three were renamed. At the species rank, 160 species were added, four were deleted, ten were moved and renamed, and 30 species were renamed. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV.
Jens H. Kuhn; Scott Adkins; Daniela Alioto; Sergey V. Alkhovsky; Gaya K. Amarasinghe; Simon J. Anthony; Tatjana Avšič-Županc; María A. Ayllón; Justin Bahl; Anne Balkema-Buschmann; Matthew J. Ballinger; Tomáš Bartonička; Christopher Basler; Sina Bavari; Martin Beer; Dennis A. Bente; Éric Bergeron; Brian H. Bird; Carol Blair; Kim R. Blasdell; Steven B. Bradfute; Rachel Breyta; Thomas Briese; Paul A. Brown; Ursula J. Buchholz; Michael J. Buchmeier; Alexander Bukreyev; Felicity Burt; Nihal Buzkan; Charles H. Calisher; Mengji Cao; Inmaculada Casas; John Chamberlain; Kartik Chandran; Rémi N. Charrel; Biao Chen; Michela Chiumenti; Il-Ryong Choi; J. Christopher S. Clegg; Ian Crozier; John V. Da Graça; Elena Dal Bó; Alberto M. R. Dávila; Juan Carlos De La Torre; Xavier De Lamballerie; Rik L. De Swart; Patrick L. Di Bello; Nicholas Di Paola; Francesco Di Serio; Ralf G. Dietzgen; Michele Digiaro; Valerian V. Dolja; Olga Dolnik; Michael A. Drebot; Jan Felix Drexler; Ralf Dürrwald; Lucie Dufkova; William G. Dundon; W. Paul Duprex; John M. Dye; Andrew J. Easton; Hideki Ebihara; Toufic Elbeaino; Koray Ergünay; Jorlan Fernandes; Anthony R. Fooks; Pierre B. H. Formenty; Leonie F. Forth; Ron A. M. Fouchier; Juliana Freitas-Astúa; Selma Gago-Zachert; George Fú Gāo; María Laura García; Adolfo García-Sastre; Aura R. Garrison; Aiah Gbakima; Tracey Goldstein; Jean-Paul J. Gonzalez; Anthony Griffiths; Martin H. Groschup; Stephan Günther; Alexandro Guterres; Roy A. Hall; John Hammond; Mohamed Hassan; Jussi Hepojoki; Satu Hepojoki; Udo Hetzel; Roger Hewson; Bernd Hoffmann; Seiji Hongo; Dirk Höper; Masayuki Horie; Holly R. Hughes; Timothy H. Hyndman; Amara Jambai; Rodrigo Jardim; Dàohóng Jiāng; Qi Jin; Gilda B. Jonson; Sandra Junglen; Serpil Karadağ; Karen E. Keller; Boris Klempa; Jonas Klingström; Gary Kobinger; Hideki Kondō; Eugene V. Koonin; Mart Krupovic; Gael Kurath; Ivan V. Kuzmin; Lies Laenen; Robert A. Lamb; Amy J. Lambert; Stanley L. Langevin; Benhur Lee; Elba R. S. Lemos; Eric M. Leroy; Dexin Li; Jiànróng Lǐ; Mifang Liang; Wénwén Liú; Yàn Liú; Igor S. Lukashevich; Piet Maes; William Marciel De Souza; Marco Marklewitz; Sergio H. Marshall; Giovanni P. Martelli; Robert R. Martin; Shin-Yi L. Marzano; Sébastien Massart; John W. McCauley; Nicole Mielke-Ehret; Angelantonio Minafra; Maria Minutolo; Ali Mirazimi; Hans-Peter Mühlbach; Elke Mühlberger; Rayapati Naidu; Tomohide Natsuaki; Beatriz Navarro; José A. Navarro; Sergey V. Netesov; Gabriele Neumann; Norbert Nowotny; Márcio R. T. Nunes; Are Nylund; Arnfinn L. Økland; Renata C. Oliveira; Gustavo Palacios; Vicente Pallas; Bernadett Pályi; Anna Papa; Colin R. Parrish; Alex Pauvolid-Corrêa; Janusz T. Pawęska; Susan Payne; Daniel R. Pérez; Florian Pfaff; Sheli R. Radoshitzky; Aziz-Ul Rahman; Pedro L. Ramos-González; Renato O. Resende; Carina A. Reyes; Bertus K. Rima; Víctor Romanowski; Gabriel Robles Luna; Paul Rota; Dennis Rubbenstroth; Jonathan A. Runstadler; Daniel Ruzek; Sead Sabanadzovic; Jiří Salát; Amadou Alpha Sall; Maria S. Salvato; Kamil Sarpkaya; Takahide Sasaya; Martin Schwemmle; Muhammad Z. Shabbir; Xiǎohóng Shí; Zhènglì Shí; Yukio Shirako; Peter Simmonds; Jana Širmarová; Manuela Sironi; Sophie Smither; Teemu Smura; Jin-Won Song; Kirsten M. Spann; Jessica R. Spengler; Mark D. Stenglein; David M. Stone; Petra Straková; Ayato Takada; Robert B. Tesh; Natalie J. Thornburg; Keizō Tomonaga; Noël Tordo; Jonathan S. Towner; Massimo Turina; Ioannis Tzanetakis; Rainer G. Ulrich; Anna Maria Vaira; Bernadette Van Den Hoogen; Arvind Varsani; Nikos Vasilakis; Martin Verbeek; Victoria Wahl; Peter J. Walker; Hui Wang; Jianwei Wang; Xifeng Wang; Lin-Fa Wang; Tàiyún Wèi; Heather Wells; Anna E. Whitfield; John V. Williams; Yuri I. Wolf; Zhìqiáng Wú; Xin Yang; Xīnglóu Yáng; Xuejie Yu; Natalya Yutin; F. Murilo Zerbini; Tong Zhang; Yong-Zhen Zhang; Guohui Zhou; Xueping Zhou. 2020 taxonomic update for phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. Archives of Virology 2020, 165, 3023 -3072.
AMA StyleJens H. Kuhn, Scott Adkins, Daniela Alioto, Sergey V. Alkhovsky, Gaya K. Amarasinghe, Simon J. Anthony, Tatjana Avšič-Županc, María A. Ayllón, Justin Bahl, Anne Balkema-Buschmann, Matthew J. Ballinger, Tomáš Bartonička, Christopher Basler, Sina Bavari, Martin Beer, Dennis A. Bente, Éric Bergeron, Brian H. Bird, Carol Blair, Kim R. Blasdell, Steven B. Bradfute, Rachel Breyta, Thomas Briese, Paul A. Brown, Ursula J. Buchholz, Michael J. Buchmeier, Alexander Bukreyev, Felicity Burt, Nihal Buzkan, Charles H. Calisher, Mengji Cao, Inmaculada Casas, John Chamberlain, Kartik Chandran, Rémi N. Charrel, Biao Chen, Michela Chiumenti, Il-Ryong Choi, J. Christopher S. Clegg, Ian Crozier, John V. Da Graça, Elena Dal Bó, Alberto M. R. Dávila, Juan Carlos De La Torre, Xavier De Lamballerie, Rik L. De Swart, Patrick L. Di Bello, Nicholas Di Paola, Francesco Di Serio, Ralf G. Dietzgen, Michele Digiaro, Valerian V. Dolja, Olga Dolnik, Michael A. Drebot, Jan Felix Drexler, Ralf Dürrwald, Lucie Dufkova, William G. Dundon, W. Paul Duprex, John M. Dye, Andrew J. Easton, Hideki Ebihara, Toufic Elbeaino, Koray Ergünay, Jorlan Fernandes, Anthony R. Fooks, Pierre B. H. Formenty, Leonie F. Forth, Ron A. M. Fouchier, Juliana Freitas-Astúa, Selma Gago-Zachert, George Fú Gāo, María Laura García, Adolfo García-Sastre, Aura R. Garrison, Aiah Gbakima, Tracey Goldstein, Jean-Paul J. Gonzalez, Anthony Griffiths, Martin H. Groschup, Stephan Günther, Alexandro Guterres, Roy A. Hall, John Hammond, Mohamed Hassan, Jussi Hepojoki, Satu Hepojoki, Udo Hetzel, Roger Hewson, Bernd Hoffmann, Seiji Hongo, Dirk Höper, Masayuki Horie, Holly R. Hughes, Timothy H. Hyndman, Amara Jambai, Rodrigo Jardim, Dàohóng Jiāng, Qi Jin, Gilda B. Jonson, Sandra Junglen, Serpil Karadağ, Karen E. Keller, Boris Klempa, Jonas Klingström, Gary Kobinger, Hideki Kondō, Eugene V. Koonin, Mart Krupovic, Gael Kurath, Ivan V. Kuzmin, Lies Laenen, Robert A. Lamb, Amy J. Lambert, Stanley L. Langevin, Benhur Lee, Elba R. S. Lemos, Eric M. Leroy, Dexin Li, Jiànróng Lǐ, Mifang Liang, Wénwén Liú, Yàn Liú, Igor S. Lukashevich, Piet Maes, William Marciel De Souza, Marco Marklewitz, Sergio H. Marshall, Giovanni P. Martelli, Robert R. Martin, Shin-Yi L. Marzano, Sébastien Massart, John W. McCauley, Nicole Mielke-Ehret, Angelantonio Minafra, Maria Minutolo, Ali Mirazimi, Hans-Peter Mühlbach, Elke Mühlberger, Rayapati Naidu, Tomohide Natsuaki, Beatriz Navarro, José A. Navarro, Sergey V. Netesov, Gabriele Neumann, Norbert Nowotny, Márcio R. T. Nunes, Are Nylund, Arnfinn L. Økland, Renata C. Oliveira, Gustavo Palacios, Vicente Pallas, Bernadett Pályi, Anna Papa, Colin R. Parrish, Alex Pauvolid-Corrêa, Janusz T. Pawęska, Susan Payne, Daniel R. Pérez, Florian Pfaff, Sheli R. Radoshitzky, Aziz-Ul Rahman, Pedro L. Ramos-González, Renato O. Resende, Carina A. Reyes, Bertus K. Rima, Víctor Romanowski, Gabriel Robles Luna, Paul Rota, Dennis Rubbenstroth, Jonathan A. Runstadler, Daniel Ruzek, Sead Sabanadzovic, Jiří Salát, Amadou Alpha Sall, Maria S. Salvato, Kamil Sarpkaya, Takahide Sasaya, Martin Schwemmle, Muhammad Z. Shabbir, Xiǎohóng Shí, Zhènglì Shí, Yukio Shirako, Peter Simmonds, Jana Širmarová, Manuela Sironi, Sophie Smither, Teemu Smura, Jin-Won Song, Kirsten M. Spann, Jessica R. Spengler, Mark D. Stenglein, David M. Stone, Petra Straková, Ayato Takada, Robert B. Tesh, Natalie J. Thornburg, Keizō Tomonaga, Noël Tordo, Jonathan S. Towner, Massimo Turina, Ioannis Tzanetakis, Rainer G. Ulrich, Anna Maria Vaira, Bernadette Van Den Hoogen, Arvind Varsani, Nikos Vasilakis, Martin Verbeek, Victoria Wahl, Peter J. Walker, Hui Wang, Jianwei Wang, Xifeng Wang, Lin-Fa Wang, Tàiyún Wèi, Heather Wells, Anna E. Whitfield, John V. Williams, Yuri I. Wolf, Zhìqiáng Wú, Xin Yang, Xīnglóu Yáng, Xuejie Yu, Natalya Yutin, F. Murilo Zerbini, Tong Zhang, Yong-Zhen Zhang, Guohui Zhou, Xueping Zhou. 2020 taxonomic update for phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. Archives of Virology. 2020; 165 (12):3023-3072.
Chicago/Turabian StyleJens H. Kuhn; Scott Adkins; Daniela Alioto; Sergey V. Alkhovsky; Gaya K. Amarasinghe; Simon J. Anthony; Tatjana Avšič-Županc; María A. Ayllón; Justin Bahl; Anne Balkema-Buschmann; Matthew J. Ballinger; Tomáš Bartonička; Christopher Basler; Sina Bavari; Martin Beer; Dennis A. Bente; Éric Bergeron; Brian H. Bird; Carol Blair; Kim R. Blasdell; Steven B. Bradfute; Rachel Breyta; Thomas Briese; Paul A. Brown; Ursula J. Buchholz; Michael J. Buchmeier; Alexander Bukreyev; Felicity Burt; Nihal Buzkan; Charles H. Calisher; Mengji Cao; Inmaculada Casas; John Chamberlain; Kartik Chandran; Rémi N. Charrel; Biao Chen; Michela Chiumenti; Il-Ryong Choi; J. Christopher S. Clegg; Ian Crozier; John V. Da Graça; Elena Dal Bó; Alberto M. R. Dávila; Juan Carlos De La Torre; Xavier De Lamballerie; Rik L. De Swart; Patrick L. Di Bello; Nicholas Di Paola; Francesco Di Serio; Ralf G. Dietzgen; Michele Digiaro; Valerian V. Dolja; Olga Dolnik; Michael A. Drebot; Jan Felix Drexler; Ralf Dürrwald; Lucie Dufkova; William G. Dundon; W. Paul Duprex; John M. Dye; Andrew J. Easton; Hideki Ebihara; Toufic Elbeaino; Koray Ergünay; Jorlan Fernandes; Anthony R. Fooks; Pierre B. H. Formenty; Leonie F. Forth; Ron A. M. Fouchier; Juliana Freitas-Astúa; Selma Gago-Zachert; George Fú Gāo; María Laura García; Adolfo García-Sastre; Aura R. Garrison; Aiah Gbakima; Tracey Goldstein; Jean-Paul J. Gonzalez; Anthony Griffiths; Martin H. Groschup; Stephan Günther; Alexandro Guterres; Roy A. Hall; John Hammond; Mohamed Hassan; Jussi Hepojoki; Satu Hepojoki; Udo Hetzel; Roger Hewson; Bernd Hoffmann; Seiji Hongo; Dirk Höper; Masayuki Horie; Holly R. Hughes; Timothy H. Hyndman; Amara Jambai; Rodrigo Jardim; Dàohóng Jiāng; Qi Jin; Gilda B. Jonson; Sandra Junglen; Serpil Karadağ; Karen E. Keller; Boris Klempa; Jonas Klingström; Gary Kobinger; Hideki Kondō; Eugene V. Koonin; Mart Krupovic; Gael Kurath; Ivan V. Kuzmin; Lies Laenen; Robert A. Lamb; Amy J. Lambert; Stanley L. Langevin; Benhur Lee; Elba R. S. Lemos; Eric M. Leroy; Dexin Li; Jiànróng Lǐ; Mifang Liang; Wénwén Liú; Yàn Liú; Igor S. Lukashevich; Piet Maes; William Marciel De Souza; Marco Marklewitz; Sergio H. Marshall; Giovanni P. Martelli; Robert R. Martin; Shin-Yi L. Marzano; Sébastien Massart; John W. McCauley; Nicole Mielke-Ehret; Angelantonio Minafra; Maria Minutolo; Ali Mirazimi; Hans-Peter Mühlbach; Elke Mühlberger; Rayapati Naidu; Tomohide Natsuaki; Beatriz Navarro; José A. Navarro; Sergey V. Netesov; Gabriele Neumann; Norbert Nowotny; Márcio R. T. Nunes; Are Nylund; Arnfinn L. Økland; Renata C. Oliveira; Gustavo Palacios; Vicente Pallas; Bernadett Pályi; Anna Papa; Colin R. Parrish; Alex Pauvolid-Corrêa; Janusz T. Pawęska; Susan Payne; Daniel R. Pérez; Florian Pfaff; Sheli R. Radoshitzky; Aziz-Ul Rahman; Pedro L. Ramos-González; Renato O. Resende; Carina A. Reyes; Bertus K. Rima; Víctor Romanowski; Gabriel Robles Luna; Paul Rota; Dennis Rubbenstroth; Jonathan A. Runstadler; Daniel Ruzek; Sead Sabanadzovic; Jiří Salát; Amadou Alpha Sall; Maria S. Salvato; Kamil Sarpkaya; Takahide Sasaya; Martin Schwemmle; Muhammad Z. Shabbir; Xiǎohóng Shí; Zhènglì Shí; Yukio Shirako; Peter Simmonds; Jana Širmarová; Manuela Sironi; Sophie Smither; Teemu Smura; Jin-Won Song; Kirsten M. Spann; Jessica R. Spengler; Mark D. Stenglein; David M. Stone; Petra Straková; Ayato Takada; Robert B. Tesh; Natalie J. Thornburg; Keizō Tomonaga; Noël Tordo; Jonathan S. Towner; Massimo Turina; Ioannis Tzanetakis; Rainer G. Ulrich; Anna Maria Vaira; Bernadette Van Den Hoogen; Arvind Varsani; Nikos Vasilakis; Martin Verbeek; Victoria Wahl; Peter J. Walker; Hui Wang; Jianwei Wang; Xifeng Wang; Lin-Fa Wang; Tàiyún Wèi; Heather Wells; Anna E. Whitfield; John V. Williams; Yuri I. Wolf; Zhìqiáng Wú; Xin Yang; Xīnglóu Yáng; Xuejie Yu; Natalya Yutin; F. Murilo Zerbini; Tong Zhang; Yong-Zhen Zhang; Guohui Zhou; Xueping Zhou. 2020. "2020 taxonomic update for phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales." Archives of Virology 165, no. 12: 3023-3072.
Fungal pathogens are seriously threatening food security and natural ecosystems; efficient and environmentally friendly control methods are essential to help safeguard such resources for increasing human populations on a global scale. Here, we find that Sclerotinia sclerotiorum, a widespread pathogen of dicotyledons, can grow endophytically in wheat, rice, barley, maize, and oat, providing protection against Fusarium head blight, stripe rust, and rice blast. Protection is also provided by disabled S. sclerotiorum strains harboring a hypovirulence virus. The disabled strain DT-8 promoted wheat yields by 4–18% in the field and consistently reduced Fusarium disease by 40–60% across multiple field trials. We term the host-dependent trophism of S. sclerotiorum, destructively pathogenic or mutualistically endophytic, as schizotrophism. As a biotroph, S. sclerotiorum modified the expression of wheat genes involved in disease resistance and photosynthesis and increased the level of IAA. Our study shows that a broad-spectrum pathogen of one group of plants may be employed as a biocontrol agent in a different group of plants where they can be utilized as beneficial microorganisms while avoiding the risk of in-field release of pathogens. Our study also raises provocative questions about the potential role of schizotrophic endophytes in natural ecosystems.
Binnian Tian; Jiatao Xie; Yanping Fu; Jiasen Cheng; Bo Li; Tao Chen; Ying Zhao; Zhixiao Gao; Puyun Yang; Martin J. Barbetti; Brett M. Tyler; Daohong Jiang. A cosmopolitan fungal pathogen of dicots adopts an endophytic lifestyle on cereal crops and protects them from major fungal diseases. The ISME Journal 2020, 14, 3120 -3135.
AMA StyleBinnian Tian, Jiatao Xie, Yanping Fu, Jiasen Cheng, Bo Li, Tao Chen, Ying Zhao, Zhixiao Gao, Puyun Yang, Martin J. Barbetti, Brett M. Tyler, Daohong Jiang. A cosmopolitan fungal pathogen of dicots adopts an endophytic lifestyle on cereal crops and protects them from major fungal diseases. The ISME Journal. 2020; 14 (12):3120-3135.
Chicago/Turabian StyleBinnian Tian; Jiatao Xie; Yanping Fu; Jiasen Cheng; Bo Li; Tao Chen; Ying Zhao; Zhixiao Gao; Puyun Yang; Martin J. Barbetti; Brett M. Tyler; Daohong Jiang. 2020. "A cosmopolitan fungal pathogen of dicots adopts an endophytic lifestyle on cereal crops and protects them from major fungal diseases." The ISME Journal 14, no. 12: 3120-3135.
We previously identified Sclerotinia sclerotiorum negative-stranded virus 1 (SsNSRV-1), the first (−) ssRNA mycovirus, associated with hypovirulence of its fungal host Sclerotinia sclerotiorum. In this study, functional analysis of Open Reading Frame Ι (ORF Ι) of SsNSRV-1 was performed. The integration and expression of ORF Ι led to defects in hyphal tips, vegetative growth, and virulence of the mutant strains of S. sclerotiorum. Further, differentially expressed genes (DEGs) responding to the expression of ORF Ι were identified by transcriptome analysis. In all, 686 DEGs consisted of 267 up-regulated genes and 419 down-regulated genes. DEGs reprogramed by ORF Ι were relevant to secretory proteins, pathogenicity, transcription, transmembrane transport, protein biosynthesis, modification, and metabolism. Alternative splicing was also detected in all mutant strains, but not in hypovirulent strain AH98, which was co-infected by SsNSRV-1 and Sclerotinia sclerotiorum hypovirus 1 (SsHV-1). Thus, the integrity of SsNSRV-1 genome may be necessary to protect viral mRNA from splicing and inactivation by the host. Taken together, the results suggested that protein ORF Ι could regulate the transcription, translation, and modification of host genes in order to facilitate viral proliferation and reduce the virulence of the host. Therefore, ORF Ι may be a potential gene used for the prevention of S. sclerotiorum.
Zhixiao Gao; Junyan Wu; Daohong Jiang; Jiatao Xie; Jiasen Cheng; Yang Lin. ORF Ι of Mycovirus SsNSRV-1 is Associated with Debilitating Symptoms of Sclerotinia sclerotiorum. Viruses 2020, 12, 456 .
AMA StyleZhixiao Gao, Junyan Wu, Daohong Jiang, Jiatao Xie, Jiasen Cheng, Yang Lin. ORF Ι of Mycovirus SsNSRV-1 is Associated with Debilitating Symptoms of Sclerotinia sclerotiorum. Viruses. 2020; 12 (4):456.
Chicago/Turabian StyleZhixiao Gao; Junyan Wu; Daohong Jiang; Jiatao Xie; Jiasen Cheng; Yang Lin. 2020. "ORF Ι of Mycovirus SsNSRV-1 is Associated with Debilitating Symptoms of Sclerotinia sclerotiorum." Viruses 12, no. 4: 456.
Recently, an increasing number of ourmia-like viruses have been found in fungi; however, the features of these viruses remain unknown. Here, we report a novel ourmia-like virus isolated from Sclerotinia sclerotiorum. This virus, named S. sclerotiorum ourmia-like virus 4 (SsOLV4), has a genome 2,982 nt in length with a G-pentamer (GGGGG) at the 5′-terminus and a C-pentamer (CCCCC) at the 3′-terminus. The SsOLV4 genome has only one large putative open reading frame (ORF) predicted with both standard codes and mitochondrial codes and encodes an RNA-dependent RNA polymerase (RdRp). SsOLV4 is closely phylogenetically related to Pyricularia oryzae ourmia-like virus 1, with 42% identity between the RdRp amino acid sequences. We constructed full-length cDNA of SsOLV4 and synthesized RNA in vitro using the T7 RNA polymerase. The synthesized RNA could transfect S. sclerotiorum protoplasts efficiently. We further found that viral RNA could infect mycelia when mixed with PEG buffer. Our study suggests that a novel genus in family Botourmiaviridae should be established for SsOLV4 and other related viruses and demonstrates that one single-stranded RNA segment encoding RdRp is sufficient for ourmia-like viruses in fungi.
Qihua Wang; Fan Mu; Jiatao Xie; Jiasen Cheng; Yanping Fu; Daohong Jiang. A Single ssRNA Segment Encoding RdRp Is Sufficient for Replication, Infection, and Transmission of Ourmia-Like Virus in Fungi. Frontiers in Microbiology 2020, 11, 379 .
AMA StyleQihua Wang, Fan Mu, Jiatao Xie, Jiasen Cheng, Yanping Fu, Daohong Jiang. A Single ssRNA Segment Encoding RdRp Is Sufficient for Replication, Infection, and Transmission of Ourmia-Like Virus in Fungi. Frontiers in Microbiology. 2020; 11 ():379.
Chicago/Turabian StyleQihua Wang; Fan Mu; Jiatao Xie; Jiasen Cheng; Yanping Fu; Daohong Jiang. 2020. "A Single ssRNA Segment Encoding RdRp Is Sufficient for Replication, Infection, and Transmission of Ourmia-Like Virus in Fungi." Frontiers in Microbiology 11, no. : 379.
Botrytis cinerea, a ubiquitous necrotrophic plant-pathogenic fungus, is responsible for grey mold and rot disease in a very wide range of plant species. Subtilisin-like proteases (or subtilases) are a very diverse family of serine proteases present in many organisms and are reported to have a broad spectrum of biological functions. Here, we identified two genes encoding subtilisin-like proteases (Bcser1 and Bcser2) in the genome of B. cinerea, both of which contain an inhibitor I9 domain and a peptidase S8 domain. The expression levels of Bcser1 and Bcser2 increased during the sclerotial forming stage, as well as during a later stage of hyphal infection on Arabidopsis thaliana leaves, but the up-regulation of Bcser1 was significantly higher than that of Bcser2. Interestingly, deletion of Bcser1 had no effect on the fungal development or virulence of B. cinerea. However, deletion of Bcser2 or double deletion of Bcser1 and Bcser2 severely impaired the hyphal growth, sclerotial formation and conidiation of B. cinerea. We also found that ∆Bcser2 and ∆Bcser1/2 could not form complete infection cushions and then lost the ability to infect intact plant leaves of Arabidopsis and tomato but could infect wounded plant tissues. Taken together, our results indicate that the subtilisin-like protease Bcser2 is crucial for the sclerotial formation, conidiation, and virulence of B. cinerea.
Xinqiang Liu; Jiatao Xie; Yanping Fu; Daohong Jiang; Tao Chen; Jiasen Cheng. The Subtilisin-Like Protease Bcser2 Affects the Sclerotial Formation, Conidiation and Virulence of Botrytis cinerea. International Journal of Molecular Sciences 2020, 21, 603 .
AMA StyleXinqiang Liu, Jiatao Xie, Yanping Fu, Daohong Jiang, Tao Chen, Jiasen Cheng. The Subtilisin-Like Protease Bcser2 Affects the Sclerotial Formation, Conidiation and Virulence of Botrytis cinerea. International Journal of Molecular Sciences. 2020; 21 (2):603.
Chicago/Turabian StyleXinqiang Liu; Jiatao Xie; Yanping Fu; Daohong Jiang; Tao Chen; Jiasen Cheng. 2020. "The Subtilisin-Like Protease Bcser2 Affects the Sclerotial Formation, Conidiation and Virulence of Botrytis cinerea." International Journal of Molecular Sciences 21, no. 2: 603.
Infection by diverse mycoviruses is a common phenomenon in Sclerotinia sclerotiorum. In this study, the full genome of a single-stranded RNA mycovirus, tentatively named Hubei sclerotinia RNA virus 1 (HuSRV1), was determined in the hypovirulent strain 277 of S. sclerotiorum. The HuSRV1 genome is 4492 nucleotides (nt) long and lacks a poly (A) tail at the 3'- terminus. Sequence analyses showed that the HuSRV1 genome contains four putative open reading frames (ORFs). ORF1a was presumed to encode a protein with a conserved protease domain and a transmembrane domain. This protein is 27% identical to the P2a protein encoded by the subterranean clover mottle virus. ORF1b encodes a protein containing a conserved RNA-dependent RNA polymerase (RdRp) domain, which may be translated into a fusion protein by a -1 ribosome frameshift. This protein is 45.9% identical to P2b encoded by the sowbane mosaic virus. ORF2 was found to encode a putative coat protein, which shares 23% identical to the coat protein encoded by the olive mild mosaic virus. ORF3 was presumed to encode a putative protein with an unknown function. Evolutionary relation analyses indicated that HuSRV1 is related to members within Sobemovirus, but forms a unique phylogenetic branch, suggesting that HuSRV1 represents a new member within Solemoviridae. HuSRV1 virions, approximately 30 nm in diameter, were purified from strain 277. The purified virions were successfully introduced into virulent strain Ep-1PNA367, resulting in a new hypovirulent strain, which confirmed that HuSRV1 confers hypovirulence on S. sclerotiorum.
Ayesha Azhar; Fan Mu; Huang Huang; Jiasen Cheng; Yanping Fu; Muhammad Rizwan Hamid; Daohong Jiang; Jiatao Xie; Mu; Fu; Xie. A Novel RNA Virus Related to Sobemoviruses Confers Hypovirulence on the Phytopathogenic Fungus Sclerotinia sclerotiorum. Viruses 2019, 11, 759 .
AMA StyleAyesha Azhar, Fan Mu, Huang Huang, Jiasen Cheng, Yanping Fu, Muhammad Rizwan Hamid, Daohong Jiang, Jiatao Xie, Mu, Fu, Xie. A Novel RNA Virus Related to Sobemoviruses Confers Hypovirulence on the Phytopathogenic Fungus Sclerotinia sclerotiorum. Viruses. 2019; 11 (8):759.
Chicago/Turabian StyleAyesha Azhar; Fan Mu; Huang Huang; Jiasen Cheng; Yanping Fu; Muhammad Rizwan Hamid; Daohong Jiang; Jiatao Xie; Mu; Fu; Xie. 2019. "A Novel RNA Virus Related to Sobemoviruses Confers Hypovirulence on the Phytopathogenic Fungus Sclerotinia sclerotiorum." Viruses 11, no. 8: 759.
Rapeseed (Brassica napus L.) is an important cruciferous crop worldwide. In this study, we collected five rapeseed samples with typical symptoms induced by phytoplasmas from Wuhan and Ezhou, Hubei Province, China, and E'erguna, Neimenggu Province, China. We successfully identified phytoplasmas from these samples via nested PCR amplification using phytoplasma-specific primer pairs. We also carried out phylogenetic analyses and grouped these phytoplasmas with the online tool iPhyClassifier. As a result, the phytoplasmas in samples EZ and NMG with witches’-broom and sample WH3 with dwarfing belonged to subgroup 16SrI–B, while the phytoplasmas in sample WH1 with fasciation and sample WH2 with peduncle clustering were members of subgroup 16SrV–H. This is the first report of phytoplasmas infecting rapeseed in China and of group 16SrV infecting rapeseed. We further investigated the host range of groups 16SrI and 16SrV and found group 16SrI in 307 plant species belonging to 230 genera of 83 families, while group 16SrV was identified in 65 species belonging to 47 genera of 30 families. The possible host expansion and host jumping of group 16SrV and the potential risk of phytoplasmas to rapeseed were also discussed.
Qian Tang; Yanping Fu; Ming Ma; Yao Yao; Zheng Qu; Jiatao Xie; Jiasen Cheng; Daohong Jiang. First report of phytoplasma groups 16SrI and 16SrV infecting Brassica napus in China. Crop Protection 2019, 126, 104921 .
AMA StyleQian Tang, Yanping Fu, Ming Ma, Yao Yao, Zheng Qu, Jiatao Xie, Jiasen Cheng, Daohong Jiang. First report of phytoplasma groups 16SrI and 16SrV infecting Brassica napus in China. Crop Protection. 2019; 126 ():104921.
Chicago/Turabian StyleQian Tang; Yanping Fu; Ming Ma; Yao Yao; Zheng Qu; Jiatao Xie; Jiasen Cheng; Daohong Jiang. 2019. "First report of phytoplasma groups 16SrI and 16SrV infecting Brassica napus in China." Crop Protection 126, no. : 104921.
Following the publication of this article [1], the authors noted the following errors.
Kai Bi; Tao Chen; Zhangchao He; Zhixiao Gao; Ying Zhao; Yanping Fu; Jiasen Cheng; Jiatao Xie; Daohong Jiang. Correction to: Proto-oncogenes in a eukaryotic unicellular organism play essential roles in plasmodial growth in host cells. BMC Genomics 2019, 20, 346 .
AMA StyleKai Bi, Tao Chen, Zhangchao He, Zhixiao Gao, Ying Zhao, Yanping Fu, Jiasen Cheng, Jiatao Xie, Daohong Jiang. Correction to: Proto-oncogenes in a eukaryotic unicellular organism play essential roles in plasmodial growth in host cells. BMC Genomics. 2019; 20 (1):346.
Chicago/Turabian StyleKai Bi; Tao Chen; Zhangchao He; Zhixiao Gao; Ying Zhao; Yanping Fu; Jiasen Cheng; Jiatao Xie; Daohong Jiang. 2019. "Correction to: Proto-oncogenes in a eukaryotic unicellular organism play essential roles in plasmodial growth in host cells." BMC Genomics 20, no. 1: 346.
We previously determined that virions of Sclerotinia sclerotiorum hypovirulence associated DNA virus 1 (SsHADV-1) could directly infect hyphae of Sclerotinia sclerotiorum, resulting in hypovirulence of the fungal host. However, the molecular mechanisms of SsHADV-1 virions disruption of the fungal cell wall barrier and entrance into the host cell are still unclear. To investigate the early response of S. sclerotiorum to SsHADV-1 infection, S. sclerotiorum hyphae were inoculated with purified SsHADV-1 virions. The pre- and post-infection hyphae were collected at one⁻three hours post-inoculation for transcriptome analysis. Further, bioinformatic analysis showed that differentially expressed genes (DEGs) regulated by SsHADV-1 infection were identified in S. sclerotiorum. In total, 187 genes were differentially expressed, consisting of more up-regulated (114) than down-regulated (73) genes. The identified DEGs were involved in several important pathways. Metabolic processes, biosynthesis of antibiotics, and secondary metabolites were the most affected categories in S. sclerotiorum upon SsHADV-1 infection. Cell structure analysis suggested that 26% of the total DEGs were related to membrane tissues. Furthermore, 10 and 27 DEGs were predicted to be located in the cell membrane and mitochondria, respectively. Gene ontology enrichment analyses of the DEGs were performed, followed by functional annotation of the genes. Interestingly, one third of the annotated functional DEGs could be involved in the Ras-small G protein signal transduction pathway. These results revealed that SsHADV-1 virions may be able to bind host membrane proteins and influence signal transduction through Ras-small G protein-coupled receptors during early infection, providing new insight towards the molecular mechanisms of virions infection in S. sclerotiorum.
Feng Ding; Jiasen Cheng; Yanping Fu; Tao Chen; Bo Li; Daohong Jiang; Jiatao Xie. Early Transcriptional Response to DNA Virus Infection in Sclerotinia sclerotiorum. Viruses 2019, 11, 278 .
AMA StyleFeng Ding, Jiasen Cheng, Yanping Fu, Tao Chen, Bo Li, Daohong Jiang, Jiatao Xie. Early Transcriptional Response to DNA Virus Infection in Sclerotinia sclerotiorum. Viruses. 2019; 11 (3):278.
Chicago/Turabian StyleFeng Ding; Jiasen Cheng; Yanping Fu; Tao Chen; Bo Li; Daohong Jiang; Jiatao Xie. 2019. "Early Transcriptional Response to DNA Virus Infection in Sclerotinia sclerotiorum." Viruses 11, no. 3: 278.
Plasmodiophora brassicae is an important biotrophic eukaryotic plant pathogen and a member of the rhizarian protists. This biotrophic pathogen causes clubroot in cruciferous plants via novel intracellular mechanisms that are markedly different from those of other biotrophic organisms. To date, genomes from six single spore isolates of P. brassicae have been sequenced. An accurate description of the evolutionary status of this biotrophic protist, however, remains lacking. Here, we determined the draft genome of the P. brassicae ZJ-1 strain. A total of 10,951 protein-coding genes were identified from a 24.1 Mb genome sequence. We applied a comparative genomics approach to prove the Rhizaria supergroup is an independent branch in the eukaryotic evolutionary tree. We also found that the GPCR signaling pathway, the versatile signal transduction to multiple intracellular signaling cascades in response to extracellular signals in eukaryotes, is significantly enriched in P. brassicae-expanded and P. brassicae-specific gene sets. Additionally, treatment with a GPCR inhibitor relieved the symptoms of clubroot and significantly suppressed the development of plasmodia. Our findings suggest that GPCR signal transduction pathways play important roles in the growth, development, and pathogenicity of P. brassicae.
Kai Bi; Tao Chen; Zhangchao He; Zhixiao Gao; Ying Zhao; Huiquan Liu; Yanping Fu; Jiatao Xie; Jiasen Cheng; Daohong Jiang. Comparative genomics reveals the unique evolutionary status of Plasmodiophora brassicae and the essential role of GPCR signaling pathways. Phytopathology Research 2019, 1, 12 .
AMA StyleKai Bi, Tao Chen, Zhangchao He, Zhixiao Gao, Ying Zhao, Huiquan Liu, Yanping Fu, Jiatao Xie, Jiasen Cheng, Daohong Jiang. Comparative genomics reveals the unique evolutionary status of Plasmodiophora brassicae and the essential role of GPCR signaling pathways. Phytopathology Research. 2019; 1 (1):12.
Chicago/Turabian StyleKai Bi; Tao Chen; Zhangchao He; Zhixiao Gao; Ying Zhao; Huiquan Liu; Yanping Fu; Jiatao Xie; Jiasen Cheng; Daohong Jiang. 2019. "Comparative genomics reveals the unique evolutionary status of Plasmodiophora brassicae and the essential role of GPCR signaling pathways." Phytopathology Research 1, no. 1: 12.
In October 2018, the order Mononegavirales was amended by the establishment of three new families and three new genera, abolishment of two genera, and creation of 28 novel species. This article presents the updated taxonomy of the order Mononegavirales as now accepted by the International Committee on Taxonomy of Viruses (ICTV).
Piet Maes; Gaya K. Amarasinghe; María A. Ayllón; Christopher F. Basler; Sina Bavari; Kim R. Blasdell; Thomas Briese; Paul A. Brown; Alexander Bukreyev; Anne Balkema-Buschmann; Ursula J. Buchholz; Kartik Chandran; Ian Crozier; Rik L. De Swart; Ralf G. Dietzgen; Olga Dolnik; Leslie L. Domier; Jan Felix Drexler; Ralf Dürrwald; William G. Dundon; W. Paul Duprex; John M. Dye; Andrew J. Easton; Anthony R. Fooks; Pierre B. H. Formenty; Ron A. M. Fouchier; Juliana Freitas-Astúa; Elodie Ghedin; Anthony Griffiths; Roger Hewson; Masayuki Horie; Julia L. Hurwitz; Timothy H. Hyndman; Dàohóng Jiāng; Gary P. Kobinger; Hideki Kondō; Gael Kurath; Ivan V. Kuzmin; Robert A. Lamb; Benhur Lee; Eric Leroy; Jiànróng Lǐ; Shin-Yi L. Marzano; Elke Mühlberger; Sergey V. Netesov; Norbert Nowotny; Gustavo Palacios; Bernadett Pályi; Janusz T. Pawęska; Susan L. Payne; Bertus K. Rima; Paul Rota; Dennis Rubbenstroth; Peter Simmonds; Sophie J. Smither; Qisheng Song; Timothy Song; Kirsten Spann; Mark D. Stenglein; David M. Stone; Ayato Takada; Robert B. Tesh; Keizō Tomonaga; Noël Tordo; Jonathan S. Towner; Bernadette Van Den Hoogen; Nikos Vasilakis; Victoria Wahl; Peter J. Walker; David Wang; Lin-Fa Wang; Anna E. Whitfield; John V. Williams; Gōngyín Yè; F. Murilo Zerbini; Yong-Zhen Zhang; Jens H. Kuhn. Taxonomy of the order Mononegavirales: second update 2018. Archives of Virology 2019, 164, 1233 -1244.
AMA StylePiet Maes, Gaya K. Amarasinghe, María A. Ayllón, Christopher F. Basler, Sina Bavari, Kim R. Blasdell, Thomas Briese, Paul A. Brown, Alexander Bukreyev, Anne Balkema-Buschmann, Ursula J. Buchholz, Kartik Chandran, Ian Crozier, Rik L. De Swart, Ralf G. Dietzgen, Olga Dolnik, Leslie L. Domier, Jan Felix Drexler, Ralf Dürrwald, William G. Dundon, W. Paul Duprex, John M. Dye, Andrew J. Easton, Anthony R. Fooks, Pierre B. H. Formenty, Ron A. M. Fouchier, Juliana Freitas-Astúa, Elodie Ghedin, Anthony Griffiths, Roger Hewson, Masayuki Horie, Julia L. Hurwitz, Timothy H. Hyndman, Dàohóng Jiāng, Gary P. Kobinger, Hideki Kondō, Gael Kurath, Ivan V. Kuzmin, Robert A. Lamb, Benhur Lee, Eric Leroy, Jiànróng Lǐ, Shin-Yi L. Marzano, Elke Mühlberger, Sergey V. Netesov, Norbert Nowotny, Gustavo Palacios, Bernadett Pályi, Janusz T. Pawęska, Susan L. Payne, Bertus K. Rima, Paul Rota, Dennis Rubbenstroth, Peter Simmonds, Sophie J. Smither, Qisheng Song, Timothy Song, Kirsten Spann, Mark D. Stenglein, David M. Stone, Ayato Takada, Robert B. Tesh, Keizō Tomonaga, Noël Tordo, Jonathan S. Towner, Bernadette Van Den Hoogen, Nikos Vasilakis, Victoria Wahl, Peter J. Walker, David Wang, Lin-Fa Wang, Anna E. Whitfield, John V. Williams, Gōngyín Yè, F. Murilo Zerbini, Yong-Zhen Zhang, Jens H. Kuhn. Taxonomy of the order Mononegavirales: second update 2018. Archives of Virology. 2019; 164 (4):1233-1244.
Chicago/Turabian StylePiet Maes; Gaya K. Amarasinghe; María A. Ayllón; Christopher F. Basler; Sina Bavari; Kim R. Blasdell; Thomas Briese; Paul A. Brown; Alexander Bukreyev; Anne Balkema-Buschmann; Ursula J. Buchholz; Kartik Chandran; Ian Crozier; Rik L. De Swart; Ralf G. Dietzgen; Olga Dolnik; Leslie L. Domier; Jan Felix Drexler; Ralf Dürrwald; William G. Dundon; W. Paul Duprex; John M. Dye; Andrew J. Easton; Anthony R. Fooks; Pierre B. H. Formenty; Ron A. M. Fouchier; Juliana Freitas-Astúa; Elodie Ghedin; Anthony Griffiths; Roger Hewson; Masayuki Horie; Julia L. Hurwitz; Timothy H. Hyndman; Dàohóng Jiāng; Gary P. Kobinger; Hideki Kondō; Gael Kurath; Ivan V. Kuzmin; Robert A. Lamb; Benhur Lee; Eric Leroy; Jiànróng Lǐ; Shin-Yi L. Marzano; Elke Mühlberger; Sergey V. Netesov; Norbert Nowotny; Gustavo Palacios; Bernadett Pályi; Janusz T. Pawęska; Susan L. Payne; Bertus K. Rima; Paul Rota; Dennis Rubbenstroth; Peter Simmonds; Sophie J. Smither; Qisheng Song; Timothy Song; Kirsten Spann; Mark D. Stenglein; David M. Stone; Ayato Takada; Robert B. Tesh; Keizō Tomonaga; Noël Tordo; Jonathan S. Towner; Bernadette Van Den Hoogen; Nikos Vasilakis; Victoria Wahl; Peter J. Walker; David Wang; Lin-Fa Wang; Anna E. Whitfield; John V. Williams; Gōngyín Yè; F. Murilo Zerbini; Yong-Zhen Zhang; Jens H. Kuhn. 2019. "Taxonomy of the order Mononegavirales: second update 2018." Archives of Virology 164, no. 4: 1233-1244.