This study examines the effect of dietary supplementation with Lactobacillus acidophilus (LA) on the cholesterol levels, immune response, and productive performance of laying hens. A total of 216, 40-week-old, commercial Hy-Line brown chicken layers were randomly assigned into four treatment groups (18 birds × three replicates per group) and fed diet supplemented with 0 (control), 1 × 10⁹, 21 × 10⁹, and 31 × 10⁹ colony forming units (CFUs) of Lactobacillus acidophilus (LA) per kg of feed for six consecutive weeks. Results show that plasma triglycerides, low-density lipoprotein (LDL) and total cholesterols became lesser, while high-density lipoprotein (HDL) cholesterol became higher in LA-supplemented groups compared to the control. In addition, a significant reduction occurred in the liver and egg yolk cholesterol by LA supplementation. Moreover, the immunological parameters including antibody titer against sheep red blood cells (SRBCs), phytohemagglutinin (PHA)-wattle swelling test, and T- & B-lymphocyte proliferation were enhanced in laying hens supplemented with LA compared to the control hens. While the heterophil to lymphocyte (H/L) ratio decreased with LA supplementation, indicating low stress conditions in the treated hens. These positive effects for LA were further reflected on the productive performance of laying hens and improved egg production, egg weight, egg mass, and feed efficiency. Our findings indicate that LA probiotic could be recommended in laying hens’ diets for lowering egg yolk cholesterol with positive impacts on health and performance.

The modern broiler industry faces huge challenges to keep high production quality and quantity, especially under environmental heat stress conditions. The negative effect of heat stress on broiler productivity is mediated by oxidative stress induction. The blue-green alga (Spirulina platensis) has many applications in poultry nutrition with the high levels of bioactive antioxidant compounds, which can alleviate the oxidative stress damage induced by high ambient temperature. The current study was designed to investigate the effects of dietary Spirulina inclusion at different levels on growth performance, redox status, carcass traits, meat quality, blood hematology, and metabolites profile of broilers subjected to cyclic heat stress. A total of 300 one-day-old Cobb-500 broiler chicks were recruited. Starting from day 21 to 42 of age, birds were randomly divided into five treatment groups with 6 replicates × 10 birds per group, where the first one was provided with the basal diet and reared under normal thermal conditions (23 ± 1 °C) to serve as a negative control. Meanwhile, the other four groups were exposed to cyclic heat stress (34 ± 1 °C for 8 h per day) and were fed a basal diet supplemented with Spirulina at a concentration of 0, 0.5, 1 or 1.5%. Spirulina supplementation to heat-stressed broilers was able to alleviate the negative impacts of heat stress on the final average daily gain, body weight and feed conversion ratio, with the best impact observed among the chickens fed 1% Spirulina. Hematological results indicate increasing hemoglobin and hematocrit levels with Spirulina supplementation compared to the non-supplemented stressed group. Further, Spirulina supplementation significantly influenced blood lipid metabolites marked by reduced serum cholesterol and low-density lipoprotein (LDL), and increased high-density lipoprotein (HDL) levels. The lipid peroxidation level was reduced (p < 0.05), while the antioxidant enzyme activity was increased with Spirulina supplementation to the heat-stressed group. Spirulina supplementation at 0.5 or 1% improved carcass dressing, breast and leg percentages. It can be concluded that dietary Spirulina supplementation at 0.5 or 1% to broiler reared under heat stress conditions can effectively improve broiler production performance and balance the redox status.

Propolis (PR) is a resin product of bee colonies that has rich bioactive antioxidant and bactericidal compounds. Endotoxin, a byproduct of bacterial growth, is reported to cause progressive induction of endogenous oxidative stress and has negative impacts on individual health and wellbeing. Hereby, we investigated the ability of PR to alleviate the oxidative stress and immunosuppression imposed by avian pathogenic Escherichia coli using laying hen as a based model. In this study, PR was dietary supplemented to hens for 4 weeks at a concentration of 0.1%. At the beginning of the 4th week of the experiment, hens from control and PR treatment were injected with E. coli (O157:H7; 10⁷ colonies/hen) or saline. The results showed significant (p < 0.05) negative impact of E. coli challenge on antioxidant status, immune response and productive performance. PR supplementation reduced (p < 0.05) inflammation markers levels (tumor necrosis factor α (TNFα) and interleukin 1β (IL-1β)) and plasma corticosterone concentration. The antioxidant status was ameliorated with dietary PR supplementation to challenged hens, showing significant (p < 0.05) reduction in malondialdehyde (MDA) levels and increasing total antioxidant capacity (TAC) concentrations. Cell mediated, as well as, humeral immune response improved significantly (p < 0.05) with dietary PR verified by the enhancement of T- and B-lymphocyte proliferation and the positive respond to phytohemagglutinin (PHA). Leucocyte cells viability increased significantly and the apoptotic factor forkhead box O3 (Foxo3) was reduced with PR supplementation. The current study revealed that dietary PR supplementation can effectively be used as an organic feed additive to overcome the endogenous oxidative stress induced by endotoxins challenge.

Heat stress (HS) induces deleterious effects on the performance of laying hens and causes economic losses for poultry industry. This study was carried out to investigate the organic effect of selenium-enriched yeast (SY) on relieving the performance, immunity and physiological deterioration induced by heat stress in laying hens. A total of 324, 28-week-old, Hy-Line Brown commercial chicken layers were randomly distributed into 4 treatments according to a 2 × 2 factorial design, with 9 hens × 9 replicates per treatment (n = 81). From 30 to 34 weeks of age, layers were exposed to 2 temperature treatments (the HS treatment groups): a thermoneutral temperature at 24°C and a heat stress at 35°C. Layers were further assigned into the 2 subgroups according to dietary supplementation with organic selenium-enriched yeast (the SY treatment groups) at either 0 or 0.4 mg/kg diet. Results indicated that all the aspects of the layer performance during the experimental period were impaired by exposure to HS, while SY supplementation improved the layer performance in both the HS and non-HS layers. Intestinal villi disruptions and liver necrotic hepatocytes were observed in the layers exposed to HS, while villi integrity and hepatocytic normality were enhanced by SY treatment. A significant (P < 0.05) decrease in the total leukocyte count, sheep red blood cell (SRBC) antibody titer, and T- and B-lymphocyte proliferation along with an increase in the heterophils/lymphocytes (H/L) ratio were observed in the HS layers compared to non-HS layers. On the contrary, SY treatment significantly (P < 0.05) improved the immune function traits in both the HS layers and non-HS layers. Furthermore, the SY treatment plays an important role in mitigating the oxidative stress and inflammation induced by HS, displaying lower levels of plasma corticosterone, lipid peroxidation, interleukin-1β, and tumor necrosis factor-α in HS layers supplemented with SY compared to HS layers without SY supplementation. These results conclude that addition of SY to the diet of laying hens could be applied as a potential nutritional approach to relieve the deterioration effects of heat stress on the immunity, physiological status, and productive performance of laying hens.

(1) Background: Propolis and bee pollen have natural bioactive compounds that may support the performance and immunological response of broilers. (2) Methods: The study included 300 1 d old Cobb-500 broiler chicks. Starting from 22–42 d of age, chicks were divided according to a 2 × 2 factorial design into one of the four treatment groups (5 replicates × 15 chicks per replicate); a basal diet without supplementation (CONT) or supplemented with 1 g/kg of propolis (PR) or bee pollen (BP) separately or in an even combination (PR + BP). (3) Results: A significant (p < 0.05) increase was obtained in the body-weight gain of broilers treated with PR, BP, and PR + BP compared to the CONT. The total antioxidant capacity and superoxide dismutase were highly (p < 0.05) activated in all treated groups compared to the CONT. Immunological parameters, especially the leukocyte cell viability, T- and B-lymphocyte proliferation, immunoglobulins (IgA and IgM), antibody titers, and wattle-swelling test were significantly (p < 0.05) enhanced in the treated broilers with PR and/or BP compared to the CONT. (4) Conclusions: The dietary supplementation of PR and/or BP could be beneficial for broiler growth through maximizing the antioxidant- and immune-system defenses.

Poultry meat production breeds are distinguished by their rapid growth, prolific output, and excellent nutritional efficiency when raised in optimal environmental conditions to realize their genetic potential. Climate change, characterized by elevated environmental temperatures, undermines optimal conditions for meat production, leading to diminished growth performance, reduced feed efficiency, and increased mortality rates, thereby resulting in substantial economic losses and critical threats to global food security. This project aims to investigate the obstacles to sustainable chicken meat production and improve economic resilience in the face of climate change, focusing on the formulation of strategies that promote sustainable food security. The global production of poultry meat rose by 1.63 million metric tons in 2023/2024 relative to 2022/2023 and is projected to grow by about 2% in 2025, attaining a new high of 104.9 million tons. Heat stress results in an annual economic loss of $2.36 billion in the U.S. chicken industry due to diminished production performance, elevated mortality rates, increased feed costs, and heightened disease resistance. Consequently, initiatives to mitigate the detrimental impacts of climate change on poultry production may include the adoption of heat-resistant breeds and the application of sustainable agricultural practices, in addition to formulating comprehensive legislation to address these issues In light of current climate change projections and rising temperatures, the research recommends the adoption of comprehensive measures to improve sustainable poultry production and ensure food security at both local and global levels.